Cytotoxicity and antiviral activity of palladium(II) and platinum(II) complexes with 2-(diphenylphosphino)benzaldehyde 1-adamantoylhydrazone

Metal coordination compounds have an important role in the development of novel drugs. Using the resazurin microtitration assay we assessed the cytotoxicity and antiviral activity of the ligand 2-(diphenylphosphino) benzaldehyde 1-adamantoylhydrazone and its Pd(II) and Pt(II) complexes. Cytotoxicity was tested in A549 human lung adenocarcinoma epithelial cells. We observed that the ligand displayed a more pronounced cytotoxic activity than the platinum-based drug, carboplatin. Morphological evaluation of A549 cells treated with the ligand by acridine orange and ethidium bromide double staining revealed the presence of signs of apoptosis. Antiviral activity against poliovirus type 1 was assessed by examination of the cytopathic effect (CPE) in Hep-2 cells. Cells that were exposed to the 19 mu M ligand before infection displayed a maximal significant reduction (by 24.42 +/- 1.49%) of the CPE. This was likely due to the inhibition of virus receptors and prevention of viral adsorption. Treatment with 17 mu M Pt(II) complex after viral infection caused a maximal significant reduction (by 30.52 +/- 3.12%) of the CPE, presumably through an effect on viral replication. The results indicate that the ligand should be viewed as a potential anticancer agent. The ligand and the Pt(II) complex show promising results for further investigation of antiviral activity

Radiation Induces Diffusible Feeder Cell Factor(s) That Cooperate with ROCK Inhibitor to Conditionally Reprogram and Immortalize Epithelial Cells

Both feeder cells and Rho kinase inhibition are required for the conditional reprogramming and immortalization of human epithelial cells. In the present study, we demonstrated that the Rho kinase inhibitor Y-27632, significantly suppresses keratinocyte differentiation and extends life span in serum-containing medium but does not lead to immortalization in the absence of feeder cells. Using Transwell culture plates, we further demonstrated that physical contact between the feeder cells and keratinocytes is not required for inducing immortalization and, more importantly, that irradiation of the feeder cells is required for this induction. Consistent with these experiments, conditioned medium was shown to induce and maintain conditionally immortalized cells, which was accompanied by increased telomerase expression. The activity of conditioned medium directly correlated with radiation-induced apoptosis of the feeder cells. Thus, the induction of conditionally reprogrammed cells is mediated by a combination of Y-27632 and a diffusible factor (or factors) released by apoptotic feeder cells

Fertility parameters and grain quality of winter barley

The experiments were performed on samples of biomass and grains of winter barley varieties (Rekord and Zlatnik) for two years. The parameters of fertility and quality (yield, mass of 1000 grains and hectoliter mass, moisture, starch, lipids, ash, cellulose and β-glucan) were monitored. The average grain yield ranged from 3,933 t ha-1 to 5,065 t ha-1. Grain yield differed significantly between years and for all genotypes and on average was higher in the first year compared to the second year of cultivation. Average mass values of 1000 grains ranged from 41.60 to 54.83 g. It was found that there are very significant differences in grain yield compared to the year of testing, while the differences between the studied barley varieties were not significant. The results of the chemical composition of barley grains have shown that the tested parameters deviated between cultivars and years. The starch composition varied from 48.8% to 50.1%, while the composition of the crude protein varied from 10.5% to 11.3%. The average composition of the lipids per dry matter was 1.68% for cultivar Rekord and 1.77% for cultivar Zlatnik, respectively. In all tested samples, the composition of the crude cellulose varied from 3.27% to 3.82%. The obtained results showed that the lowest composition of the crude ashes was recorded in the sample of cultivar Rekord (1.58%), while the highest was detected in the sample of cultivar Zlatnik (1.70%). The composition of moisture ranged from 10.11% to 11.01%, while composition of β-glucan varied from 3.9% to 4.23%

Bioakumulacija i translokacija metala u vrsti Alyssum markgrafii O. E. Schulz

The aim of this study was to determine the concentrations of 7 metals (Ca, Mg, Mn, Fe, Zn, Ni, Cr) in the soil and selected plant species Alyssum markgrafii O. E. Schulz in one serpentinite sites in the foothills of the mountain Goc. The concentrations of Ni and Cr in the investigated soil were above the maximum allowable concentration of substances in the soil, also above limit and remediation values for a given metals in the soil, according to regulation of Republic of Serbia. The great contents of Ni, Cr and Fe in plant tissues of species A. markgrafii were determined. Good translocation of Ca and Zn from under-ground to above-ground organs of species A. markgrafii has also been shown, and it can be considered suitable for the phytoextraction of investigated metals.Cilj ovog rada bio je da se odrede koncentracije nekih metala (Ca, Mg, Mn, Fe, Zn, Ni, Cr) u zemljištu i vrsti Alyssum markgrafii O. E. Schulz na lokalitetu u podnožju planine Goč. Koncentracije Ni i Cr u istraživanom zemljištu prelazile su propisane maksimalno dozvoljene koncentracije, granične vrednosti, kao i remedijacione vrednosti ovih metala u zemljištu, saglasno uredbi i pravilniku Republike Srbije. Listovi vrste A. markgrafii akumulirali su najviše Mg, Mn, Fe, Ni i Cr. Stablo ove vrste je sadržalo najviše Zn, a cvast najviše Ca. Utvrđeni su veliki sadržaji Ni, Cr i Fe u biljnim tkivima vrste A. markgrafii. Pokazana je i dobra translokacija Ca i Zn od podzemnih ka nadzemnim organima vrste A. markgrafii, kao i da se ona može smatrati pogodnom za fitoekstrakciju ispitivanih metala

SADRŽAJ METALA U ZEMLJIŠTU I ODABRANIM BILJKAMA NA JALOVIŠTU FLOTACIJE RUDNIK DOO „RUDNIK"

The aim of this study was to determine the concentration of some metals (Ca, Mg, Mn, Fe, Zn, Ni, Cr, Cd) in the soil and species of Tussilago farfara L. and Clematis vitalba L. on the tailings and flotation of the mine DOO "Rudnik". Concentrations in the soil were: Pb and Cu - higher than the maximum allowed, limit and remediation values; Cd, Cr and Ni - higher than the maximum allowed and limit values, and Zn higher than the limit values (according to the regulations of the Republic of Serbia). Better bioaccumulation of all tested metals was shown by T. farfara species, especially leaves for Zn. The results indicate the possible use of aboveground parts of both plant species in the revitalization of tailings by bioaccumulation of tested metals.Publishe

Liberty County Strategic Plan 2016 - 2036

In the fall of 2015, the County of Liberty and Texas Target Communities partnered to create a task force to represent the community. The task force was integral to the planning process, contributing the thoughts, desires, and opinions of community members—as well as their enthusiasm about Liberty’s future. This fourteen-month planning process ended in August 2016. The result of this collaboration is the County of Liberty Strategic Plan, which is the official policy guide for the community’s growth over the next twenty years.Liberty Strategic Plan 2036 provides a guide for the future growth of the county. This document was developed by Texas Target Communities in partnership with the County of Liberty

Search for High-Mass Resonances Decaying to τν in pp Collisions at √s=13 TeV with the ATLAS Detector

A search for high-mass resonances decaying to τν using proton-proton collisions at √s=13 TeV produced by the Large Hadron Collider is presented. Only τ-lepton decays with hadrons in the final state are considered. The data were recorded with the ATLAS detector and correspond to an integrated luminosity of 36.1 fb−1. No statistically significant excess above the standard model expectation is observed; model-independent upper limits are set on the visible τν production cross section. Heavy W′ bosons with masses less than 3.7 TeV in the sequential standard model and masses less than 2.2–3.8 TeV depending on the coupling in the nonuniversal G(221) model are excluded at the 95% credibility level

Measurement of jet fragmentation in Pb+Pb and pp collisions at √s NN =5.02 TeV with the ATLAS detector

This paper presents a measurement of jet fragmentation functions in 0.49 nb −1 of Pb+Pb collisions and 25 pb −1 of pp collisions at √ sNN =5.02 TeV collected in 2015 with the ATLAS detector at the LHC. These measurements provide insight into the jet quenching process in the quark-gluon plasma created in the aftermath of ultra-relativistic collisions between two nuclei. The modifications to the jet fragmentation functions are quantified by dividing the measurements in Pb+Pb collisions by baseline measurements in pp collisions. This ratio is studied as a function of the transverse momentum of the jet, the jet rapidity, and the centrality of the collision. In both collision systems, the jet fragmentation functions are measured for jets with transverse momentum between 126 GeV and 398 GeV and with an absolute value of jet rapidity less than 2.1. An enhancement of particles carrying a small fraction of the jet momentum is observed, which increases with centrality and with increasing jet transverse momentum. Yields of particles carrying a very large fraction of the jet momentum are also observed to be enhanced. Between these two enhancements of the fragmentation functions a suppression of particles carrying an intermediate fraction of the jet momentum is observed in Pb+Pb collisions. A small dependence of the modifications on jet rapidity is observed