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    6243 research outputs found

    Challenges and potential for detecting and quantifying titanium dioxide in food

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    Titanium dioxide (TiO2) is banned in some countries but its use is still permitted in others. The global food supply chain is therefore challenged with the need to use rapid and reliable testing methods to either detect the presence of TiO2 or to quantify its concentration. The goal of this study was to determine the feasibility of using color, texture profile analysis, Raman microscopy, and X-ray fluorescence (XRF) spectroscopy to detect and quantify TiO2 in fillings used in the pastry and confectionery industry. In this study, two types of fillings were investigated: vanilla based and chocolate based. All fillings were prepared in four different variations – without TiO2 and with three concentrations as follows: 0.25 g*kg-1, 0.5 g*kg-1, or 0.75 g*kg-1 TiO2 per sample. The methods were selected for their ability to analyze the samples in a short period of time. All of the methods showed moderate to high potential for detecting TiO2 in the samples. The results reveal how TiO2 affects the food matrix color and texture. Use of Raman microscopy confirms its detectability, although concentrations of TiO2 do not follow a pattern. X-ray fluorescence spectroscopy showed the greatest potential as it can not only detect TiO2 but can also quantify its concentration in the samples. The highest potential for quantifying the concentration of this food additive was achieved with XRF.This is the peer-reviewed version of the article: Đekić, I., Lević, S., Šmigić, N., Bouleau, A., Ilijević, K., Roganović, J.,& Rakić, V.. (2024). Challenges and potential for detecting and quantifying titanium dioxide in food. in Journal of the Science of Food and Agriculture, n/a. [https://doi.org/10.1002/jsfa.13356

    Blue-light-driven photoactivity of L-cysteine-modified graphene quantum dots and their antibacterial effects

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    The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure

    Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties

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    Research data for the unpublished paper: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. Densitometry analysis of ovalbumin band intensity after digestion in the presence of microplastics on SDS-PAGE gels.Related to research data no. 1: [https://cherry.chem.bg.ac.rs/handle/123456789/6465]This data set belongs to unpublished manuscript "Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties"Related to published version: [https://cherry.chem.bg.ac.rs/handle/123456789/6510

    Do post-translational and processing-born food protein modifications affect protein digestibility and their immune properties?

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    Protein post-translational modifications and those induced by food processing (PTMs) occur in many forms and can widely influence protein structure and behaviour. Yet, their structural and functional aspects in protein architecture are mainly overlooked. Until recently, this was mostly a consequence of insurmountable obstacles related to their global proteome identification and quantification via mass spectrometry-based proteomics. However, recent advancement in high-resolution tandem mass spectrometry, coupled with dedicated software, such as PEAKS Studio for an unspecified identification of PTMs, enabled their confident mapping. In our recent works we have established a method for global, open and relative quantitative profiling of PTMs without enrichment step, demonstrating its usefulness in environmental (1), biomedical (2) and food technology (3) sciences. PTMs could influence enzyme hydrolytic efficiency. This was a starting point to grow the idea of porcine trypsin being used in proteomics to serve as a probe to decipher differences in scissile bond hydrolysis caused by PTMs, based on steric and charge changes introduced as a possible hindrance or facilitation to its active site. We further hypothesized that the effects observed would be even more pronounced with human trypsin, since it is less efficient compared to the porcine counterpart. Therefore, we have reassessed our porcine-derived trypsin-generated proteomic data of the major peanut allergen Ara h 1 from the raw and roasted peanut, to look for possible facilitating/hindrance effects on trypsin digestion efficacy caused by PTMs positioned on K/R residues, by developing a manual method to analyse the extent of trypsin hydrolytic efficiency on modified and unmodified sequences. The algorithm based on machine learning of the big proteomic data in public repositories could be made to determine enzyme cleavage efficiency in relation to PTMs presence based on our developed manual method and it could be applied in other life science fields. This topic is important for understanding of peanut (food) allergy and human gastrointestinal digestion of proteins and PTMs introduced by food processin

    Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study

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    Simple and scalable synthetic approach was used for the preparation of thirteen novel tacrine derivatives consisting of tacrine and N-aryl-piperidine-4-carboxamide moiety connected by a five-methylene group linker. An anti-Alzheimer disease (AD) potential of newly designed tacrine derivatives was evaluated against two important AD targets, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In vitro pharmacological evaluation showed strong ChE inhibitory activity of all compounds, with IC50 values ranging from 117.5 to 455 nM for AChE and 34 to 324 nM for BuChE. As a representative of the series with the best cytotoxicity / ChE inhibitory activity ratio, expressed as the selectivity index (SI), 2-chlorobenzoyl derivative demonstrated mixed-type inhibition on AChE and BuChE, suggesting binding to both CAS and PAS of the enzymes. It also exhibited antioxidant capacity and neuroprotective potential against amyloid-β (Aβ) toxicity in the culture of neuron-like cells. In-depth computational analysis corroborated well with in vitro ChE inhibition, illuminating that all compounds exhibit significant potential in targeting both enzymes. Molecular dynamics (MD) simulations revealed that 2-chlorobenzoyl derivative, created complexes with AChE and BuChE that demonstrated sufficient stability throughout the observed MD simulation. Computationally predicted ADME properties indicated that these compounds should have good blood–brain barrier (BBB) permeability, an important factor for CNS-targeting drugs. Overall, all tested compounds showed promising pharmacological behavior, highlighting the multi-target potential of 2- chlorobenzoyl derivative which should be further investigated as a new lead in the drug development process

    Blue-light-driven photoactivity of L-cysteine-modified graphene quantum dots and their antibacterial effects

    No full text
    The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L-cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure

    Primeri interdisciplinarnih aktivnosti za unapređenje ekološke pismenosti učenika

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    Razumevanje ekosistema i procesa koji se dešavaju u životnoj sredini, predviđeno ekološkim obrazovanjem učenika, zahteva saradnju i timski rad nastavnika iz različitih disciplina. To podrazumeva primenu modela interdisciplinarnog pristupa učenju i nastavi. U okviru projekta ELIPS procenjeno je trenutno stanje nivoa ekološke pismenosti učenika sedmog razreda osnovne škole u Srbiji, uz određivanje faktora koji doprinose razvoju ekološkog identiteta učenika. Od mogućih 240 poena na međunarodnom upitniku koji meri nivo ekološke pismenosti, MSELS, učenici iz Srbije (n=877) ostvarili su prosečno 148 poena. Kako bi nastavnici prirodnih predmeta u osnovnoj školi imali gotove alate za razvoj pro-ekološkog ponašanja i brige o životnoj sredini, članice tima projekta ELIPS razvijaju nastavni materijal u funkciji poboljšanja ekoloških promišljanja, kao i razvoja ekološkog senzibiliteta svih učesnika u procesu obrazovanja. U ovom radu su data uputstva za različite jednostavne aktivnosti u nastavi biologije i hemije, kojim bi učenici bolje razumeli ekološke probleme. Jedna od predloženih aktivnosti se može realizovati upotrebom lako dostupnog pribora i materijala, kao što su latice crvene ruže, sumpor u prahu, providna staklena posuda, odgovarajući poklopac i šibice. U eksperimentu kojim se pokazuje efekat oksida sumpora iz kiselih kiša na pigmente u cveću, učenici mogu da na razumljiv način shvate negativan efekat kiselih kiša na prirodu, uz podsticanje diskusije o značaju pigmenata u biljkama. Ova aktivnost može inspirisati učenike da sami prepoznaju druge primere zagađenja životne sredine, kao što su ispuštanje neprečišćenih otpadnih voda ili uticaj nepravilno odloženog opasnog otpada na kvalitet zemljišta. Horizontalna i vertikalna integracija sadržaja prirodnih nauka, kroz navođenje primera ekoloških problema i izvođenje jednostavnih eksperimenata, može olakšati učenicima razumevanje prirode i uloge čoveka u njoj, što je ključno za negovanje njihovog ekološkog identiteta.Presentation: [https://cherry.chem.bg.ac.rs/handle/123456789/6495

    Challenges and potential for detecting and quantifying titanium dioxide in food

    No full text
    Titanium dioxide (TiO2) is banned in some countries but its use is still permitted in others. The global food supply chain is therefore challenged with the need to use rapid and reliable testing methods to either detect the presence of TiO2 or to quantify its concentration. The goal of this study was to determine the feasibility of using color, texture profile analysis, Raman microscopy, and X-ray fluorescence (XRF) spectroscopy to detect and quantify TiO2 in fillings used in the pastry and confectionery industry. In this study, two types of fillings were investigated: vanilla based and chocolate based. All fillings were prepared in four different variations – without TiO2 and with three concentrations as follows: 0.25 g*kg-1, 0.5 g*kg-1, or 0.75 g*kg-1 TiO2 per sample. The methods were selected for their ability to analyze the samples in a short period of time. All of the methods showed moderate to high potential for detecting TiO2 in the samples. The results reveal how TiO2 affects the food matrix color and texture. Use of Raman microscopy confirms its detectability, although concentrations of TiO2 do not follow a pattern. X-ray fluorescence spectroscopy showed the greatest potential as it can not only detect TiO2 but can also quantify its concentration in the samples. The highest potential for quantifying the concentration of this food additive was achieved with XRF.Peer-reviewed manuscript: [https://cherry.chem.bg.ac.rs/handle/123456789/6508

    Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals

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    Y0.8−xGdxF3:Yb/Er mesocrystals with a biocompatible surface and diverse morphological characteristics were successfully synthesized using chitosan-assisted solvothermal processing. Their structural properties, studied using X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning and transmission electron microscopy and energy dispersive X-ray analysis, were further correlated with the up-conversion emission (λexc = 976 nm) recorded in function of temperature. Based on the change in the visible green emissions originating from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, the corresponding LIR was acquired in the physiologically relevant range of temperatures (25–50 °C). The detected absolute sensitivity of about 0.0042 °C−1, along with the low cytotoxicity toward both normal human lung fibroblasts (MRC-5) and cancerous lung epithelial (A549) cells, indicate a potential for use in temperature sensing in biomedicine. Additionally, their enhanced internalization in cells, without suppression of cell viability, enabled in vitro labeling of cancer and healthy cells upon 976 nm laser irradiation

    Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets

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    The experimental data for the results shown in the manuscript "Microplastic contamination of edible parts of commercially important mussels, clams and shrimps in different markets". Statistical investigation of the presence of microplastics in bivalve species using microFTIR. This dataset includes samples from various origins, including Sebia, South Korea, Croatia and Belgium, and includes species as diverse as shrimp, mussels and clams

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    Faculty of Chemistry Repository - Cherry
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