Burkholderia lata Infections from Intrinsically Contaminated Chlorhexidine Mouthwash, Australia, 2016

"Emerging Infectious Diseases is an open access journal in the public domain"Burkholderia lata was isolated from 8 intensive care patients at 2 tertiary hospitals in Australia. Whole-genome sequencing demonstrated that clinical and environmental isolates originated from a batch of contaminated commercial chlorhexidine mouthwash. Genomic analysis identified efflux pump–encoding genes as potential facilitators of bacterial persistence within this biocide

Fire regulates the abundance of alien plant species around roads and settlements in the Serengeti National Park

A large portion of East African ecosystems are officially protected, yet increasing wildlife tourism and infrastructural development are exposing these areas to invasion by alien plant species. To date there has been little quantification of alien plant species in the Serengeti National Park, Tanzania. In this study, we aimed to: (1) establish a list of common alien plant species; (2) quantify the frequencies of alien species near roads and settlements (i.e. tourist lodges and a campsite), and (3) estimate the abundance (plant cover) of alien plant species in relation to source activities (i.e. gardening) and park management (i.e. fire). In total, we detected 15 alien plant species in our surveys with an 80% probability of encountering an alien species within the first 50 m from a road or settlement. Overall, we found no difference in the presence of alien species near roads or settlements, but did find a significant decline in species presence with distance from these sources. Cumulative fire frequency was the most important factor influencing the abundance of alien species with the highest alien plant cover in areas with infrequent or no fires over the last 13 years. There was no difference in alien plant cover in relation to other commonly cited source activities, which may be due to the stronger influence of fire. Although the abundance of the majority of alien plant species was negatively related to fire, some species, notably Tagetes minuta, had higher cover with more frequent fires. Our results contradict findings from other African savannahs that suggest fire promotes invasive species and this is likely due to the species-specific interactions with fire. In the Serengeti, fire will be difficult to use as a management tool due to variable species response. Thus, we highlight that other management approaches such as physical removal and biological control agents can be implemented; however future work with these techniques should also consider the interaction of alien plant species with fire

Establishment of a large semi-field system for experimental study of African malaria vector ecology and control in Tanzania

<b>Background:</b> Medical entomologists increasingly recognize that the ability to make inferences between laboratory experiments of vector biology and epidemiological trends observed in the field is hindered by a conceptual and methodological gap occurring between these approaches which prevents hypothesis-driven empirical research from being conducted on relatively large and environmentally realistic scales. The development of Semi-Field Systems (SFS) has been proposed as the best mechanism for bridging this gap. Semi-field systems are defined as enclosed environments, ideally situated within the natural ecosystem of a target disease vector and exposed to ambient environmental conditions, in which all features necessary for its life cycle completion are present. Although the value of SFS as a research tool for malaria vector biology is gaining recognition, only a few such facilities exist worldwide and are relatively small in size (< 100 m<sup>2</sup>). <b>Methods:</b> The establishment of a 625 m<sup>2</sup> state-of-the-art SFS for large-scale experimentation on anopheline mosquito ecology and control within a rural area of southern Tanzania, where malaria transmission intensities are amongst the highest ever recorded, is described. <b>Results:</b> A greenhouse frame with walls of mosquito netting and a polyethylene roof was mounted on a raised concrete platform at the Ifakara Health Institute. The interior of the SFS was divided into four separate work areas that have been set up for a variety of research activities including mass-rearing for African malaria vectors under natural conditions, high throughput evaluation of novel mosquito control and trapping techniques, short-term assays of host-seeking behaviour and olfaction, and longer-term experimental investigation of anopheline population dynamics and gene flow within a contained environment that simulates a local village domestic setting. <b>Conclusion:</b> The SFS at Ifakara was completed and ready for use in under two years. Preliminary observations indicate that realistic and repeatable observations of anopheline behaviour are obtainable within the SFS, and that habitat and climatic features representative of field conditions can be simulated within it. As work begins in the SFS in Ifakara and others around the world, the major opportunities and challenges to the successful application of this tool for malaria vector research and control are discussed