Análisis bioinformático y predicción de genes en secuencias genómicas de clostridium sp. ibun22a

Este trabajo tuvo como propósito identificar secuencias de genes en clones obtenidos en una librería genómica de la cepa colombiana de Clostridium sp. IBUN 22A. Los insertos de nueve clones con tamaæos superiores a 500 pb fueron secuenciados y analizados por medio de bÅ“squedas de los insertos completos o de sus marcos abiertos de lectura (ORFs) traducidos en GenBank 141.0 y Uniprot 6.6 con BLAST 2.2.8. Se identificaron seis genes con alta similaridad a genes de metabolismo bÆsico (housekeeping) en diferentes especies de Clostridium. En el clon pBsIBUN22A-1 se localizó una secuencia de 851 pb con una identidad del 99.74% respecto al gen codificante de la enzima glicerol deshidratasa (DhaBl) de Clostridium butyricum (AY968605) involucrada en la producción de 1,3 Propanodiol (1,3-PD). La identifica­ción de genes presentes en la cepa nativa Clostridium IBUN 22A abre la puerta a la investigación básica y a la ingeniería metabólica para hacer más rentable el proceso de producción de 1,3-PD junto al conocimiento de los genes presentes en la cepa nativa. Palabras clave: análisis de secuencias, librería genómica, Clostridium, glicerol deshidratasa, 1,3-propanodiol.This work was aimed at identifying gene sequences in recombinant clones obtained from a genomic library from the Colombian Clostridium sp. IBUN 22A native strain. Nine clones greater than 500 bp were sequenced and analysed using BLAST 2.2.8 to search for complete inserts or their translated open reading frames (ORFs) in GenBank 141.0 and Uniprot 6.6. Seven genes having high similarity to housekeeping genes from different Clostridium species were identified. An 851 bp sequence was located in the pBsIBUN22A-1 clone, having 99.74% identity with the gene encoding the Clostridium butyricum enzyme glycerol dehydratase (DhaBl) which is involved in 1,3-propanediol (1,3-PD) production. Identifying genes in the native IBUN 22A strain formed the starting point for basic research and metabolic engineering aimed at making 1,3-PD production more profitable and increasing knowledge of genes present in the native strain. Key words: glycerol dehydratase, 1,3-propanediol, genomic library, Clostridium, sequence analysis

Innovación turística y desarrollo regional

Libro que se compila 14 capítulos asociados a las temáticas de innovación y desarrollo tecnológico en el sector turístico, emprendimiento y tecnologías emergentes, políticas estratégicas y tácticas para mejorar la competitividad en las empresas, políticas públicas y educación para el desarrollo regional y responsabilidad social ambientalBook that compiles 14 chapters associated to the themes of innovation and technological development in the tourism sector, entrepreneurship and emerging technologies, strategic and tactical policies to improve competitiveness in companies, public policies and education for regional development and environmental social responsibilityInnovación social como estrategia para el desarrollo de las poblaciones palafíticas de la ciénaga grande de santa marta mediante la oferta de productos turísticos / Gregoria Polo de Lobatón; José Luis Rosenstiehl Martínez; Daulis Lobatón Polo -- Uso de las tic para mejorar la competitividad turística en San Basilio de Palenque / Jesús Llerena Cabrera; Raúl José Martelo Gómez; David Franco Borré -- Plataforma web niu (nuevo, ideas y usados) / Néstor José Ocampo Ardila -- Posicionamiento estratégico del turismo urbano de eventos como atributo de la personalidad del espacio territorial de la ciudad de santa marta / Zuleidy María Ruíz Torres -- Identificación competitiva de la oferta exportadora de la región ariari para fortalecer el desarrollo social y económico en zonas de posconflicto basado en el comercio exterior y logística / Keyla Karina González Martínez -- Tecnologías integradas en gestión sostenible de operadores turísticos en zonas de posconflicto del departamento del meta / Carlos Hernán Cruz Castro; óscar Eduardo Sarmiento Saavedra -- Herramienta online de control de ingresos y facturación: una solución dinámica para las pequeñas y medianas empresas / Esmerlis Camargo Torres; Antonio José González Liñán; Marieth Orcasitas Peñaloza; Yerson Monroy Contreras -- Modelo de cadena productiva dinamizado a través de la educación y las estrategias TIC para el desarrollo regional sostenible en Risaralda / Beatriz Elena Franco Cárdenas; Patricia Henao Montoya; Marco Aurelio Aristizabal Valencia -- Evaluación físico - química de adhesivo de yuca (manihot esculenta) como alternativa comercial para la Orinoquia / Yarithza Molina Caro; Wilfran Hernán Cortes Conde -- Formulaciones nutracéuticas alimenticias para estilos de vida saludables / Daldo Araujo Vidal; Daniel Mendoza Cujia; Maresa Anaya de oro -- Competencias tecnológicas como estrategia formativa en los aprendices de gestión de redes de datos del SENA regional guajira / Carlos Antonio Salas Solano; Alejandro Jesús Osorio Amaya; Alda Pérez Campuzano; Duvan Andrés rondón bravo -- Uso y apropiación del computador como herramienta para las prácticas educativas de los docentes / Alda Pérez Campuzano; Carlos Antonio Salas Solano; Elkin Fuentes Jiménez; Kira Rodríguez Moscote -- El aviturismo como eje transformador de cultura ambiental y desarrollo sustentable / Lina María Gamarra Pineda; Néstor Alejandro Tascón Arias -- Huella ecológica (he), indicador de condición ambiental para evaluar la sostenibilidad en instituciones de educación (ie) / Cristian David Trujillo CardonaPrimera ediciónna169 página

La magia de tus pensamientos : poesías & cuentos

Recopilación de poesías y cuentos realizados por aprendices del SENA de los programas de Mantenimiento de Motores Diesel, Técnico en Sistemas, Automatización Industrial, Electricidad Industrial, Diseño e Integración de Automatismos Mecatrónicos, Mantenimiento de Equipo Biomédico, Animación 3D, Manejo Ambiental, Diseño e integración de Automatismos Mecatrónicos y Mantenimiento mecatrónico automotriz, entre 2015 y 2017.Poesías 2018 -- Dándome tu vida / Brayan Orlando Jaimes Chaparro -- Tierra infinita / Christian Ricardo Caballero Villamizar -- Lamento a la tierra / Jeferson Alexis Montoya Moreno -- Conciencia por favor / Daniel Rincón -- Salvar un hogar llamado tierra / William Gamboa Arguello -- Planeta tierra / Iván Arley jurado -- Salvemos nuestro planeta / Jonathan David Silva -- Tierra bella / Martin Santiago -- Mi planeta / Fabián Moreno -- Generando conciencia / José Alberto Lizarazo -- Añoranza / Javier Casanova -- Sin tierra… / Gustavo Andrés Rodríguez Mendoza -- Planeta vivo y limpio / Yesid Serrano -- Palabras del destino / Osneider David Hernández Otalvaro -- Planeta tierra / Luis Fernando Isidro -- Medio ambiente / Yorgin David Hernández -- Prevenir / Brayan Mauricio Sanmiguel Luengas -- Nada será cómo antes / Jaime Andres Rodriguez Parrado -- Yo…Sol / Jonathan Bautista -- Lamento / Héctor Mauricio Parra Cuesta -- Nuestro hogar / Juan José Amaya -- Te agradecemos / Edwin Andrés Navarro Rozo, Anderson Cardozo Villamizar -- HErmoso milagro / Jhonatan Jaimes Solano -- El creador / Ana Rosa Hernández Santana -- Cuentos 2015 -- Juventud en la zona azul / Jeniffer Solano Ardila -- Los Ucayali y los Cubeo / Oscar Ivan Rueda Quintero -- Vientos de armonía / Ever Edinson Monsalve Salcedo -- El nacimiento de una nueva era / Carlos Leonardo Ardila Alvarado -- Luz de sueños / Hugo Andrés Álvarez -- Sol de medio día / Claudia Ramírez Guarín -- Cuentos 2016 -- Mucha verraquera mano / Héctor Josué Amado Sandoval -- El viaje de irse y nunca volver / Dayana Marcela Fuentes Duran -- “Santander es hermosa” / Kelly Tatiana Pabón Blanco -- Los valores del SENA / Ana Rosa Hernandez Santana -- La fuerza del espiritu y el deseo de cambiar / Pablo Andrés Capera Rodríguez -- SENA, paz y reconciliación / Adrián Benítez R., Rubén Darío Martínez -- Cuentos 2017 -- Cartas a un pasado / Angelita Delgado Pérez -- ¿Por qué así? ¿por qué tan blanco? ¿por qué no de otra manera? / Cristian Felipe Vargas Buenahora -- El gato pacho pacho, el gato más pacho de todos los pachos / Edwin Matajira García -- Freeland “el paraíso perdido” / Dámaso Antonio Morales Palacin -- Un mundo al revés / Fabio Eduardo Contreras Piñeresna91 página

Optimasi Portofolio Resiko Menggunakan Model Markowitz MVO Dikaitkan dengan Keterbatasan Manusia dalam Memprediksi Masa Depan dalam Perspektif Al-Qur`an

Risk portfolio on modern finance has become increasingly technical, requiring the use of sophisticated mathematical tools in both research and practice. Since companies cannot insure themselves completely against risk, as human incompetence in predicting the future precisely that written in Al-Quran surah Luqman verse 34, they have to manage it to yield an optimal portfolio. The objective here is to minimize the variance among all portfolios, or alternatively, to maximize expected return among all portfolios that has at least a certain expected return. Furthermore, this study focuses on optimizing risk portfolio so called Markowitz MVO (Mean-Variance Optimization). Some theoretical frameworks for analysis are arithmetic mean, geometric mean, variance, covariance, linear programming, and quadratic programming. Moreover, finding a minimum variance portfolio produces a convex quadratic programming, that is minimizing the objective function ðð¥with constraintsð ð 𥠥 ðandð´ð¥ = ð. The outcome of this research is the solution of optimal risk portofolio in some investments that could be finished smoothly using MATLAB R2007b software together with its graphic analysis

Search for stop and higgsino production using diphoton Higgs boson decays

Results are presented of a search for a "natural" supersymmetry scenario with gauge mediated symmetry breaking. It is assumed that only the supersymmetric partners of the top-quark (stop) and the Higgs boson (higgsino) are accessible. Events are examined in which there are two photons forming a Higgs boson candidate, and at least two b-quark jets. In 19.7 inverse femtobarns of proton-proton collision data at sqrt(s) = 8 TeV, recorded in the CMS experiment, no evidence of a signal is found and lower limits at the 95% confidence level are set, excluding the stop mass below 360 to 410 GeV, depending on the higgsino mass

Juxtaposing BTE and ATE – on the role of the European insurance industry in funding civil litigation

One of the ways in which legal services are financed, and indeed shaped, is through private insurance arrangement. Two contrasting types of legal expenses insurance contracts (LEI) seem to dominate in Europe: before the event (BTE) and after the event (ATE) legal expenses insurance. Notwithstanding institutional differences between different legal systems, BTE and ATE insurance arrangements may be instrumental if government policy is geared towards strengthening a market-oriented system of financing access to justice for individuals and business. At the same time, emphasizing the role of a private industry as a keeper of the gates to justice raises issues of accountability and transparency, not readily reconcilable with demands of competition. Moreover, multiple actors (clients, lawyers, courts, insurers) are involved, causing behavioural dynamics which are not easily predicted or influenced. Against this background, this paper looks into BTE and ATE arrangements by analysing the particularities of BTE and ATE arrangements currently available in some European jurisdictions and by painting a picture of their respective markets and legal contexts. This allows for some reflection on the performance of BTE and ATE providers as both financiers and keepers. Two issues emerge from the analysis that are worthy of some further reflection. Firstly, there is the problematic long-term sustainability of some ATE products. Secondly, the challenges faced by policymakers that would like to nudge consumers into voluntarily taking out BTE LEI

Differential cross section measurements for the production of a W boson in association with jets in proton–proton collisions at √s = 7 TeV

Measurements are reported of differential cross sections for the production of a W boson, which decays into a muon and a neutrino, in association with jets, as a function of several variables, including the transverse momenta (pT) and pseudorapidities of the four leading jets, the scalar sum of jet transverse momenta (HT), and the difference in azimuthal angle between the directions of each jet and the muon. The data sample of pp collisions at a centre-of-mass energy of 7 TeV was collected with the CMS detector at the LHC and corresponds to an integrated luminosity of 5.0 fb[superscript −1]. The measured cross sections are compared to predictions from Monte Carlo generators, MadGraph + pythia and sherpa, and to next-to-leading-order calculations from BlackHat + sherpa. The differential cross sections are found to be in agreement with the predictions, apart from the pT distributions of the leading jets at high pT values, the distributions of the HT at high-HT and low jet multiplicity, and the distribution of the difference in azimuthal angle between the leading jet and the muon at low values.United States. Dept. of EnergyNational Science Foundation (U.S.)Alfred P. Sloan Foundatio

Penilaian Kinerja Keuangan Koperasi di Kabupaten Pelalawan

This paper describe development and financial performance of cooperative in District Pelalawan among 2007 - 2008. Studies on primary and secondary cooperative in 12 sub-districts. Method in this stady use performance measuring of productivity, efficiency, growth, liquidity, and solvability of cooperative. Productivity of cooperative in Pelalawan was highly but efficiency still low. Profit and income were highly, even liquidity of cooperative very high, and solvability was good

Severe early onset preeclampsia: short and long term clinical, psychosocial and biochemical aspects

Preeclampsia is a pregnancy specific disorder commonly defined as de novo hypertension and proteinuria after 20 weeks gestational age. It occurs in approximately 3-5% of pregnancies and it is still a major cause of both foetal and maternal morbidity and mortality worldwide1. As extensive research has not yet elucidated the aetiology of preeclampsia, there are no rational preventive or therapeutic interventions available. The only rational treatment is delivery, which benefits the mother but is not in the interest of the foetus, if remote from term. Early onset preeclampsia (<32 weeks’ gestational age) occurs in less than 1% of pregnancies. It is, however often associated with maternal morbidity as the risk of progression to severe maternal disease is inversely related with gestational age at onset2. Resulting prematurity is therefore the main cause of neonatal mortality and morbidity in patients with severe preeclampsia3. Although the discussion is ongoing, perinatal survival is suggested to be increased in patients with preterm preeclampsia by expectant, non-interventional management. This temporising treatment option to lengthen pregnancy includes the use of antihypertensive medication to control hypertension, magnesium sulphate to prevent eclampsia and corticosteroids to enhance foetal lung maturity4. With optimal maternal haemodynamic status and reassuring foetal condition this results on average in an extension of 2 weeks. Prolongation of these pregnancies is a great challenge for clinicians to balance between potential maternal risks on one the eve hand and possible foetal benefits on the other. Clinical controversies regarding prolongation of preterm preeclamptic pregnancies still exist – also taking into account that preeclampsia is the leading cause of maternal mortality in the Netherlands5 - a debate which is even more pronounced in very preterm pregnancies with questionable foetal viability6-9. Do maternal risks of prolongation of these very early pregnancies outweigh the chances of neonatal survival? Counselling of women with very early onset preeclampsia not only comprises of knowledge of the outcome of those particular pregnancies, but also knowledge of outcomes of future pregnancies of these women is of major clinical importance. This thesis opens with a review of the literature on identifiable risk factors of preeclampsia