Nueva metodología para probar el sistema nervioso autónomo en individuos hipertensos Sistema nervioso autónomo en hipertensos

RESUMEN Introducción y objetivo: Actualmente el Sistema Nervioso Autónomo está siendo apuntado como un factor importante en la génesis y desarrollo de la hipertensión arterial sistémica. Por esto, objetivamos estudiar el Sistema Nervioso Autónomo en esta patología, a través de una nueva metodología que utilice la frecuencia cardiacaMetodología: 45 sujetos fueron seleccionados (12 hipertensos y 33 sanos) para verificación de la presión arterial y frecuencia cardiaca a cada latido (2,5 min en reposo y 2,5 min después del ortostatismo). Falsos valores fueron retirados de las series temporales para posterior interpolación, debido a la falta de periodicidad de los latidos cardiacos. Posterior submuestro a 10Hz fue realizado y un filtro que respeta la frecuencia cardiaca fue aplicado. Análisis espectral en las series temporales fue realizado para posterior estimación de medianas y cuartiles. Finalmente, test de hipótesis con Wilcoxon rank sum test para verificar diferencias estadísticas entre grupos fue realizado.Resultados: Las medianas de las potencias porcentuales en las altas frecuencias, pre y post maniobra, de sanos fueron ligeramente inferiores pero no estadísticamente significativas al compararlas con los hipertensos (sanos: 42,69 y 32,39, hipertensos: 46,91 y 33,99). De otro lado, el mismo estimador en las bajas frecuencias fue levemente superior en sanos (sanos: 57,30 y 67,60 e hipertensos: 53,09 y 66). Adicionalmente, la diferencia en la respuesta autonómica de los hipertensos no fue significativa (p>0,01) mientras que en los sanos  esto si ocurrió.Conclusiones: El método ha demostrado potencial para identificar disfunción del autónomo en hipertensión. También se confirma una activación del simpático disminuida en sujetos hipertensos cuando se realiza una maniobra ortostática. Salud UIS 2010; 42:   Palabras clave: Barorreceptores, hipertensión arterial sistémica, sistema nervioso autónomo, electrofisiología ABSTRACT Introduction and objective: Currently the Autonomous Nervous System has been considered as an important factor in the genesis and the development of Arterial Systemic Hypertension. Because of that, aimed to study the Autonomous Nervous Sistem in this pathology through a new methodology which uses heart rate.Methodology: 45 subjects were selected (12 hypertensive and 31 healthy) to check the arterial pressure and heart rate beat by beat (2.5 min in rest and 2.5 min after the orthostatism) spurious values were retired from temporal series for interpolation because the lack of heart rate periodicity.  Then, sub sampling in 10Hz was realized and a filter that respect the heart rate was applied. Spectral analysis in the temporal series was realized by posterior median and quartile estimation. Finally, hypothesis test with Wilcoxon rank sum test to check statistical differences between groups was performed.Results: The medians of the percentage powers by the high frequencies, pre and post maneuver, in healthy individuals were a few inferior but not with statistical significance when are compared with hypertensive individuals (healthy:42.69 and 32.39; hypertensive:46.91 and 33.99). On the other hand, the same estimator by the low frequencies was a few superior in healthy individuals (healthy: 57.30 and 67.60; hypertensive: 53.09 and 66). Additionally, the difference in the autonomic response between hypertensive individuals was not significative (p>0.01) meanwhile in healthy individuals do.Conclusions: The methodology had demonstrated potential to identify autonomic disfunction in hypertensive. Also, it confirms a lower sympathetic activation in hypertensive individuals when the orthostatic maneuver is done. Salud UIS 2010; 42:   Keywords: Baroreceptors, arterial systemic hypertension, autonomic nervous system, electrophysiolog

Nueva metodología para probar el sistema nervioso autónomo en individuos hipertensos Sistema nervioso autónomo en hipertensos

RESUMEN Introducción y objetivo: Actualmente el Sistema Nervioso Autónomo está siendo apuntado como un factor importante en la génesis y desarrollo de la hipertensión arterial sistémica. Por esto, objetivamos estudiar el Sistema Nervioso Autónomo en esta patología, a través de una nueva metodología que utilice la frecuencia cardiacaMetodología: 45 sujetos fueron seleccionados (12 hipertensos y 33 sanos) para verificación de la presión arterial y frecuencia cardiaca a cada latido (2,5 min en reposo y 2,5 min después del ortostatismo). Falsos valores fueron retirados de las series temporales para posterior interpolación, debido a la falta de periodicidad de los latidos cardiacos. Posterior submuestro a 10Hz fue realizado y un filtro que respeta la frecuencia cardiaca fue aplicado. Análisis espectral en las series temporales fue realizado para posterior estimación de medianas y cuartiles. Finalmente, test de hipótesis con Wilcoxon rank sum test para verificar diferencias estadísticas entre grupos fue realizado.Resultados: Las medianas de las potencias porcentuales en las altas frecuencias, pre y post maniobra, de sanos fueron ligeramente inferiores pero no estadísticamente significativas al compararlas con los hipertensos (sanos: 42,69 y 32,39, hipertensos: 46,91 y 33,99). De otro lado, el mismo estimador en las bajas frecuencias fue levemente superior en sanos (sanos: 57,30 y 67,60 e hipertensos: 53,09 y 66). Adicionalmente, la diferencia en la respuesta autonómica de los hipertensos no fue significativa (p>0,01) mientras que en los sanos  esto si ocurrió.Conclusiones: El método ha demostrado potencial para identificar disfunción del autónomo en hipertensión. También se confirma una activación del simpático disminuida en sujetos hipertensos cuando se realiza una maniobra ortostática. Salud UIS 2010; 42:   Palabras clave: Barorreceptores, hipertensión arterial sistémica, sistema nervioso autónomo, electrofisiología ABSTRACT Introduction and objective: Currently the Autonomous Nervous System has been considered as an important factor in the genesis and the development of Arterial Systemic Hypertension. Because of that, aimed to study the Autonomous Nervous Sistem in this pathology through a new methodology which uses heart rate.Methodology: 45 subjects were selected (12 hypertensive and 31 healthy) to check the arterial pressure and heart rate beat by beat (2.5 min in rest and 2.5 min after the orthostatism) spurious values were retired from temporal series for interpolation because the lack of heart rate periodicity.  Then, sub sampling in 10Hz was realized and a filter that respect the heart rate was applied. Spectral analysis in the temporal series was realized by posterior median and quartile estimation. Finally, hypothesis test with Wilcoxon rank sum test to check statistical differences between groups was performed.Results: The medians of the percentage powers by the high frequencies, pre and post maneuver, in healthy individuals were a few inferior but not with statistical significance when are compared with hypertensive individuals (healthy:42.69 and 32.39; hypertensive:46.91 and 33.99). On the other hand, the same estimator by the low frequencies was a few superior in healthy individuals (healthy: 57.30 and 67.60; hypertensive: 53.09 and 66). Additionally, the difference in the autonomic response between hypertensive individuals was not significative (p>0.01) meanwhile in healthy individuals do.Conclusions: The methodology had demonstrated potential to identify autonomic disfunction in hypertensive. Also, it confirms a lower sympathetic activation in hypertensive individuals when the orthostatic maneuver is done. Salud UIS 2010; 42:   Keywords: Baroreceptors, arterial systemic hypertension, autonomic nervous system, electrophysiolog

Drug release properties of diflunisal from layer-by-layer self-assembled kappa-carrageenan/chitosan nanocapsules: effect of deposited layers

Engineering of multifunctional drug nanocarriers combining stability and good release properties remains a great challenge. In this work, natural polymers kappa-carrageenan (kappa-CAR) and chitosan (CS) were deposited onto olive oil nanoemulsion droplets (NE) via layer-by-layer (LbL) self-assembly to study the release mechanisms of the anti-inflammatory diflunisal (DF) as a lipophilic drug model. The nano-systems were characterized by dynamic light scattering (DLS), zeta potential (zeta-potential) measurements, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray energy dispersive spectroscopy (XEDS) and Fourier transform infrared spectroscopy (FTIR) to confirm the NE-coating with polymer layers. In addition, kinetic release studies of DF were developed by the dialysis diffusion bag technique. Mathematical models were applied to investigate the release mechanisms. The results showed that stable and suitably sized nanocapsules (similar to 300 nm) were formed. Also, the consecutive adsorption of polyelectrolytes by charge reversal was evidenced. More interestingly, the drug release mechanism varied depending on the number of layers deposited. The nanosized systems containing up to two layers showed anomalous transport and first order kinetics. Formulations with three and four layers exhibited Case II transport releasing diflunisal with zero order kinetics

Ice-nucleating particles in a coastal tropical site

Atmospheric aerosol particles that can nucleate ice are referred to as ice-nucleating particles (INPs). Recent studies have confirmed that aerosol particles emitted by the oceans can act as INPs. This very relevant information can be included in climate and weather models to predict the formation of ice in clouds, given that most of them do not consider oceans as a source of INPs. Very few studies that sample INPs have been carried out in tropical latitudes, and there is a need to evaluate their availability to understand the potential role that marine aerosol may play in the hydrological cycle of tropical regions. This study presents results from the first measurements obtained during a field campaign conducted in the tropical village of Sisal, located on the coast of the Gulf of Mexico of the Yucatan Peninsula in Mexico in January–February 2017, and one of the few data sets currently available at such latitudes (i.e., 21∘&thinsp;N). Aerosol particles sampled in Sisal are shown to be very efficient INPs in the immersion freezing mode, with onset freezing temperatures in some cases as high as −3&thinsp;∘C, similarly to the onset temperature from Pseudomonas syringae. The results show that the INP concentration in Sisal was higher than at other locations sampled with the same type of INP counter. Air masses arriving in Sisal after the passage of cold fronts have surprisingly higher INP concentrations than the campaign average, despite their lower total aerosol concentration. The high concentrations of INPs at warmer ice nucleation temperatures (T&gt;-15&thinsp;∘C) and the supermicron size of the INPs suggest that biological particles may have been a significant contributor to the INP population in Sisal during this study. However, our observations also suggest that at temperatures ranging between −20 and −30&thinsp;∘C mineral dust particles are the likely source of the measured INPs.</p

Antisperm Antibody Testing: A Comprehensive Review of Its Role in the Management of Immunological Male Infertility and Results of a Global Survey of Clinical Practices

Antisperm antibodies (ASA), as a cause of male infertility, have been detected in infertile males as early as 1954. Multiple causes of ASA production have been identified, and they are due to an abnormal exposure of mature germ cells to the immune system. ASA testing (with mixed anti-globulin reaction, and immunobead binding test) was described in the WHO manual 5th edition and is most recently listed among the extended semen tests in the WHO manual 6th edition. The relationship between ASA and infertility is somewhat complex. The presence of sperm agglutination, while insufficient to diagnose immunological infertility, may indicate the presence of ASA. However, ASA can also be present in the absence of any sperm agglutination. The andrological management of ASA depends on the etiology and individual practices of clinicians. In this article, we provide a comprehensive review of the causes of ASA production, its role in immunological male infertility, clinical indications of ASA testing, and the available therapeutic options. We also provide the details of laboratory procedures for assessment of ASA together with important measures for quality control. Additionally, laboratory and clinical scenarios are presented to guide the reader in the management of ASA and immunological male infertility. Furthermore, we report the results of a recent worldwide survey, conducted to gather information about clinical practices in the management of immunological male infertility

Technical aspects and clinical limitations of sperm DNA fragmentation testing in male infertility: a global survey, current guidelines, and expert recommendations.

PURPOSE: Sperm DNA fragmentation (SDF) is a functional sperm abnormality that can impact reproductive potential, for which four assays have been described in the recently published sixth edition of the WHO laboratory manual for the examination and processing of human semen. The purpose of this study was to examine the global practices related to the use of SDF assays and investigate the barriers and limitations that clinicians face in incorporating these tests into their practice. MATERIALS AND METHODS: Clinicians managing male infertility were invited to complete an online survey on practices related to SDF diagnostic and treatment approaches. Their responses related to the technical aspects of SDF testing, current professional society guidelines, and the literature were used to generate expert recommendations via the Delphi method. Finally, challenges related to SDF that the clinicians encounter in their daily practice were captured. RESULTS: The survey was completed by 436 reproductive clinicians. Overall, terminal deoxynucleotidyl transferase deoxyuridine triphosphate Nick-End Labeling (TUNEL) is the most commonly used assay chosen by 28.6%, followed by the sperm chromatin structure assay (24.1%), and the sperm chromatin dispersion (19.1%). The choice of the assay was largely influenced by availability (70% of respondents). A threshold of 30% was the most selected cut-off value for elevated SDF by 33.7% of clinicians. Of respondents, 53.6% recommend SDF testing after 3 to 5 days of abstinence. Although 75.3% believe SDF testing can provide an explanation for many unknown causes of infertility, the main limiting factors selected by respondents are a lack of professional society guideline recommendations (62.7%) and an absence of globally accepted references for SDF interpretation (50.3%). CONCLUSIONS: This study represents the largest global survey on the technical aspects of SDF testing as well as the barriers encountered by clinicians. Unified global recommendations regarding clinician implementation and standard laboratory interpretation of SDF testing are crucial

Constraints on jet quenching in p-Pb collisions at root s(NN)=5.02 TeV measured by the event-activity dependence of semi-inclusive hadron-jet distributions

CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFINEP - FINANCIADORA DE ESTUDOS E PROJETOSFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOThe ALICE Collaboration reports the measurement of semi-inclusive distributions of charged-particle jets recoiling from a high-transverse momentum trigger hadron in p-Pb collisions at root s(NN) = 5.02TeV. Jets are reconstructed from charged-particle tracks using the anti-k(T) algorithm with resolution parameter R = 0.2 and 0.4. A data-driven statistical approach is used to correct the uncorrelated background jet yield. Recoil jet distributions are reported for jet transverse momentum 15 < p(T,jet)(ch) < 50 GeV/c and are compared in various intervals of p-Pb event activity, based on charged-particle multiplicity and zero-degree neutral energy in the forward (Pb-going) direction. The semi-inclusive observable is self-normalized and such comparisons do not require the interpretation of p-Pb event activity in terms of collision geometry, in contrast to inclusive jet observables. These measurements provide new constraints on the magnitude of jet quenching in small systems at the LHC. In p-Pb collisions with high event activity, the average medium-induced out-of-cone energy transport for jets with R = 0.4 and 15 < p(T,jet)(ch) < 50 GeV/c is measured to be less than 0.4 GeV/c at 90% confidence, which is over an order of magnitude smaller than a similar measurement for central Pb-Pb collisions at root s(NN) = 2.76 TeV. Comparison is made to theoretical calculations of jet quenching in small systems, and to inclusive jet measurements in p-Pb collisions selected by event activity at the LHC and in d-Au collisions at RHIC.78395113CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFINEP - FINANCIADORA DE ESTUDOS E PROJETOSFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFINEP - FINANCIADORA DE ESTUDOS E PROJETOSFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOSem informaçãoSem informaçãoSem informaçãoAgências de fomento estrangeiras apoiaram essa pesquisa, mais informações acesse artig

Measurement of Z(0)-boson production at large rapidities in Pb-Pb collisions at root s(NN)=5.02 TeV

The production of Z(0) bosons at large rapidities in Pb-Pb collisions at root s(NN) = 5.02 TeV is reported. Z(0) candidates are reconstructed in the dimuon decay channel (Z(0) -> mu(+) mu(-)), based on muons selected with pseudo-rapidity -4.0 20 GeV/c. The invariant yield and the nuclear modification factor, RAA, are presented as a function of rapidity and collision centrality. The value of R-AA for the 0-20% central Pb-Pb collisions is 0.67 +/- 0.11(stat.) +/- 0.03 (syst.) f 0.06(corr. syst.), exhibiting a deviation of 2.6 sigma from unity. The results are well-described by calculations that include nuclear modifications of the parton distribution functions, while the predictions using vacuum PDFs deviate from data by 2.3 sigma in the 0-90% centrality class and by 3 sigma in the 0-20% central collisions. (C) 2018 The Author(s). Published by Elsevier B.V.Peer reviewe

Measurement of deuteron spectra and elliptic flow in Pb-Pb collisions at root s(NN)=2.76 TeV at the LHC

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFINANCIADORA DE ESTUDOS E PROJETOS - FINEPFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPThe transverse momentum (p(T)) spectra and elliptic flow coefficient (v(2)) of deuterons and anti-deuterons at mid-rapidity (|y| < 0.5) are measured with the ALICE detector at the LHC in Pb-Pb collisions at root s(NN) = 2.76 TeV. The measurement of the p(T) spectra of (anti-)deuterons is done up to 8 GeV/c in 0-10% centrality class and up to 6 GeV/c in 10-20% and 20-40% centrality classes. The v(2) is measured in the 0.8 < p(T) < 5 GeV/c interval and in six different centrality intervals (0-5, 5-10, 10-20, 20-30, 30-40 and 40-50%) using the scalar product technique. Measured pi(+/-), K-+/- and p+(p) over bar transverse-momentum spectra and v(2) are used to predict the deuteron p(T) spectra and v(2) within the Blast-Wave model. The predictions are able to reproduce the v(2) coefficient in the measured p(T) range and the transverse-momentum spectra for p(T) gt; 1.8 GeV/c within the experimental uncertainties. The measurement of the coalescence parameter B-2 is performed, showing a p(T) dependence in contrast with the simplest coalescence model, which fails to reproduce also the measured v(2) coefficient. In addition, the coalescence parameter B-2 and the elliptic flow coefficient in the 20-40% centrality interval are compared with the AMPT model which is able, in its version without string melting, to reproduce the measured v(2)(p(T)) and the B-2(p(T)) trend.7710120CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFINANCIADORA DE ESTUDOS E PROJETOS - FINEPFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFINANCIADORA DE ESTUDOS E PROJETOS - FINEPFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informaçãoSem informaçãoSem informaçãoThe ALICE Collaboration would like to thank all its engineers and technicians for their invaluable contributions to the construction of the experiment and the CERN accelerator teams for the outstanding performance of the LHC complex. The ALICE Collaboration gratefully acknowledges the resources and support provided by all Grid centres and the Worldwide LHC Computing Grid (WLCG) collaboration. The ALICE Collaboration acknowledges the following funding agencies for their support in building and running the ALICE detector: A. I. Alikhanyan National Science Laboratory (Yerevan Physics Institute) Foundation (ANSL), State Committee of Science and World Federation of Scientists (WFS), Armenia; Austrian Academy of Sciences and Nationalstiftung für Forschung, Technologie und Entwicklung, Austria; Ministry of Communications and High Technologies, National Nuclear Research Center, Azerbaijan; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Universidade Federal do Rio Grande do Sul (UFRGS), Financiadora de Estudos e Projetos (Finep) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Brazil; Ministry of Science and Technology of China (MSTC), National Natural Science Foundation of China (NSFC) and Ministry of Education of China (MOEC), China; Ministry of Science, Education and Sport and Croatian Science Foundation, Croatia; Ministry of Education, Youth and Sports of the Czech Republic, Czech Republic; The Danish Council for Independent Research|Natural Sciences, the Carlsberg Foundation and Danish National Research Foundation (DNRF), Denmark; Helsinki Institute of Physics (HIP), Finland; Commissariat à l’Energie Atomique (CEA) and Institut National de Physique Nucléaire et de Physique des Particules (IN2P3) and Centre National de la Recherche Scientifique (CNRS), France; Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie (BMBF) and GSI Helmholtzzentrum für Schwerionenforschung GmbH, Germany; General Secretariat for Research and Technology, Ministry of Education, Research and Religions, Greece; National Research, Development and Innovation Office, Hungary; Department of Atomic Energy Government of India (DAE) and Council of Scientific and Industrial Research (CSIR), New Delhi, India; Indonesian Institute of Science, Indonesia; Centro Fermi-Museo Storico della Fisica e Centro Studi e Ricerche Enrico Fermi and Istituto Nazionale di Fisica Nucleare (INFN), Italy; Institute for Innovative Science and Technology, Nagasaki Institute of Applied Science (IIST), Japan Society for the Promotion of Science (JSPS) KAKENHI and Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan; Consejo Nacional de Ciencia (CONACYT) y Tecnología, through Fondo de Cooperación Internacional en Ciencia y Tecnología (FONCICYT) and Dirección General de Asuntos del Personal Academico (DGAPA), Mexico; Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO), Netherlands; The Research Council of Norway, Norway; Commission on Science and Technology for Sustainable Development in the South (COMSATS), Pakistan; Pontificia Universidad Católica del Perú, Peru; Ministry of Science and Higher Education and National Science Centre, Poland; Korea Institute of Science and Technology Information and National Research Foundation of Korea (NRF), Republic of Korea; Ministry of Education and Scientific Research, Institute of Atomic Physics and Romanian National Agency for Science, Technology and Innovation, Romania; Joint Institute for Nuclear Research (JINR), Ministry of Education and Science of the Russian Federation and National Research Centre Kurchatov Institute, Russia; Ministry of Education, Science, Research and Sport of the Slovak Republic, Slovakia; National Research Foundation of South Africa, South Africa; Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), Cubaenergía, Cuba, Ministerio de Ciencia e Innovacion and Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT), Spain; Swedish Research Council (VR) and Knut and Alice Wallenberg Foundation (KAW), Sweden; European Organization for Nuclear Research, Switzerland; National Science and Technology Development Agency (NSDTA), Suranaree University of Technology (SUT) and Office of the Higher Education Commission under NRU project of Thailand, Thailand; Turkish Atomic Energy Agency (TAEK), Turkey; National Academy of Sciences of Ukraine, Ukraine; Science and Technology Facilities Council (STFC), United Kingdom; National Science Foundation of the United States of America (NSF) and United States Department of Energy, Office of Nuclear Physics (DOE NP), United States of America

First measurement of Ξc0 production in pp collisions at s=7 TeV

The production of the charm-strange baryon Csi0c is measured for the first time at the LHC via its semileptonic decay into e+Csi-νe in pp collisions at sqrt(s) = 7 TeV with the ALICE detector. The transverse momentum (pT) differential cross section multiplied by the branching ratio is presented in the interval 1 &lt; pT &lt; 8 GeV/c at mid-rapidity, |y| &lt; 0.5. The transverse momentum dependence of the Csi0c baryon production relative to the D0 meson production is compared to predictions of event generators with various tunes of the hadronisation mechanism, which are found to underestimate the measured cross- section ratio.The production of the charm-strange baryon Csi0c is measured for the first time at the LHC via its semileptonic decay into e+Csi-νe in pp collisions at sqrt(s) = 7 TeV with the ALICE detector. The transverse momentum (pT) differential cross section multiplied by the branching ratio is presented in the interval 1 &lt; pT &lt; 8 GeV/c at mid-rapidity, |y| &lt; 0.5. The transverse momentum dependence of the Csi0c baryon production relative to the D0 meson production is compared to predictions of event generators with various tunes of the hadronisation mechanism, which are found to underestimate the measured cross- section ratio