PenQuest Volume 3, Number 2

Table of Contents for this Volume: Untitled by Joe Avanzini Woodcutting on Lost Mountain by Tess Gallagher Untitled by Judith Mizrahi Women I have loved by Dottie Fletcher Untitled by Rick Wagner Untold Stories by William Slaughter Untitled by Steve Balunan Two German Women by Dottie Fletcher Untitled by Anne Calloway Tourists by Carol Grimes Untitled by Win Lyons Rollin\u27 Bones by Barbara Ritchey Untitled by Steve Balunan Hannukah Harbor by Jerry Nelson Night-letter by William Slaughter Untitled by Bruce Abbey Domestic by Carol Grimes Untitled by Steve Balunan The Storm Pit by Howard Denson Untitled by Judith Mizrahi Hattie by Dottie Fletcher Untitled by Linda Willcox Untitled by Helen Hagador

PenQuest Volume 3, Number 1

The Table of Contents for this Volume: Untitled by R. Bruce Warner Seeing the Unseen by Sharon Gresham Untitled by Helen Hagadorn Untitled by Steve Balunan Happy Holidays by Donna Kaluzniak River-walking, Night-talking by William Slaughter Ribbon of Light by Judith Mizrahi Love in Parentheses by Sharon Gresham Untitled by Steve Balunan Protohistory by Patricia Kraft Untitled by Bruce Abbey Untitled by Rick Wagner Thanatopsis by Pat Kraft Untitled by Sue Hightower Untitled by Rick Wagner Conversations of a Woman by Sharon Gresham Thur, Fri, Sat, at Mr. B\u27s by Patricia Kraft Untitled by Rick Wagner Untitled by Cindy Carlisle Untitled by Win Lyons Untitled by Cindy Carlisle Untitled by Modesta Matthews Untitled by James Tutten A Light at Mill Pond Crossing by Joe Palmer Untitled by Rick Wagner Two People by Kathleen Gay Untitled by Rick Wagner American Dream (Russian Version) by William Slaughter Untitled by Judith Mizrahi Untitled by Linda Willco

A host KH RNA-binding protein is a susceptibility factor targeted by an RXLR effector to promote late blight disease

Plant pathogens deliver effector proteins that alter host processes to create an environment conducive to colonization. Attention has focused on identifying the targets of effectors and how their manipulation facilitates disease. RXLR effector Pi04089 from the potato blight pathogen Phytophthora infestans accumulates in the host nucleus and enhances colonization when transiently expressed in planta. Its nuclear localization is required for enhanced P. infestans colonization. Pi04089 interacts in yeast and in planta with a putative potato K-homology (KH) RNA-binding protein, StKRBP1. Co-localization of Pi04089 and StKRBP1, and bimolecular fluorescence complementation between them, indicate they associate at nuclear speckles. StKRBP1 protein levels increased when it was co-expressed with Pi04089. Indeed, such accumulation of StKRBP1 was observed also on the first day of leaf colonization by the pathogen. Remarkably, overexpression of StKRBP1 significantly enhances P. infestans infection. Mutation of the nucleotide-binding motif GxxG to GDDG in all three KH domains of StKRBP1 abolishes its interaction with Pi04089, its localization to nuclear speckles, and its increased accumulation when co-expressed with the effector. Moreover, the mutant StKRBP1 protein no longer enhances leaf colonization by P. infestans, implying that nucleotide binding is likely required for this activity. We thus argue that StKRBP1 can be regarded as a susceptibility factor, as its activity is beneficial to the pathogen

Metaphors we die by? Geoengineering, metaphors and the argument from catastrophe

Geoeengineering the climate by reflecting sunlight or extracting carbon dioxide from the atmosphere has attracted increasing attention from natural scientists, social scientists, policy makers and the media. This article examines promotional discourse related to geoengineering from the 1980s to 2010. It asks in particular how this option for dealing with the problems posed by climate change were framed through the use of conceptual and discourse metaphors and whether one can argue that these are metaphors we ‘live by’ or metaphors we might ‘die by’. Findings show that an overarching argument from catastrophe was bolstered by three conceptual master-metaphors, namely The Planet is a body, The Planet is a machine and The planet is a patient/addict, linked to a variety of discourse metaphors, older conceptual metaphors and clichés. This metaphorical landscape began to shift while the article was being written and will have to be closely monitored in the future

Testing associations between cannabis use and subcortical volumes in two large population-based samples

Background and aims Disentangling the putative impact of cannabis on brain morphology from other comorbid substance use is critical. After controlling for the effects of nicotine, alcohol and multi‐substance use, this study aimed to determine whether frequent cannabis use is associated with significantly smaller subcortical grey matter volumes. Design Exploratory analyses using mixed linear models, one per region of interest (ROI), were performed whereby individual differences in volume (outcome) at seven subcortical ROIs were regressed onto cannabis and comorbid substance use (predictors). Setting Two large population‐based twin samples from the United States and Australia. Participants A total of 622 young Australian adults [66% female; μage = 25.9, standard deviation SD) = 3.6] and 474 middle‐aged US males (μage = 56.1SD = 2.6) of predominately Anglo‐Saxon ancestry with complete substance use and imaging data. Subjects with a history of stroke or traumatic brain injury were excluded. Measurements Magnetic resonance imaging (MRI) and volumetric segmentation methods were used to estimate volume in seven subcortical ROIs: thalamus, caudate nucleus, putamen, pallidum, hippocampus, amygdala and nucleus accumbens. Substance use measurements included maximum nicotine and alcohol use, total life‐time multi‐substance use, maximum cannabis use in the young adults and regular cannabis use in the middle‐aged males. Findings After correcting for multiple testing (P = 0.007), cannabis use was unrelated to any subcortical ROI. However, maximum nicotine use was associated with significantly smaller thalamus volumes in middle‐aged males. Conclusions In exploratory analyses based on young adult and middle‐aged samples, normal variation in cannabis use is unrelated statistically to individual differences in brain morphology as measured by subcortical volume