457 research outputs found
Conductance of amyloid β based peptide filaments: structure–function relations.
Controlling the morphology of self-assembled peptide nanostructures, particularly those based on
amyloid peptides, has been the focus of intense research. In order to exploit these structures in
electronic applications, further understanding of their electronic behavior is required. In this work, the
role of peptide morphology in determining electronic conduction along self-assembled peptide
nanofilament networks is demonstrated. The peptides used in this work were based on the sequence
AAKLVFF, which is an extension of a core sequence from the amyloid b peptide. We show that the
incorporation of a non-natural amino acid, 2-thienylalanine, instead of phenylalanine improves the
obtained conductance with respect to that obtained for a similar structure based on the native sequence,
which was not the case for the incorporation of 3-thienylalanine. Furthermore, we demonstrate that the
morphology of the self-assembled structures, which can be controlled by the solvent used in the
assembly process, strongly affects the conductance, with larger conduction obtained for a morphology
of long, straight filaments. Our results demonstrate that, similar to natural systems, the assembly and
folding of peptides could be of great importance for optimizing their function as components of
electronic devices. Hence, sequence design and assembly conditions can be used to control the
performance of peptide based structures in such electronic applications
DNA as a universal substrate for chemical kinetics
Molecular programming aims to systematically engineer molecular and chemical systems of autonomous function and ever-increasing complexity. A key goal is to develop embedded control circuitry within a chemical system to direct molecular events. Here we show that systems of DNA molecules can be constructed that closely approximate the dynamic behavior of arbitrary systems of coupled chemical reactions. By using strand displacement reactions as a primitive, we construct reaction cascades with effectively unimolecular and bimolecular kinetics. Our construction allows individual reactions to be coupled in arbitrary ways such that reactants can participate in multiple reactions simultaneously, reproducing the desired dynamical properties. Thus arbitrary systems of chemical equations can be compiled into real chemical systems. We illustrate our method on the Lotka–Volterra oscillator, a limit-cycle oscillator, a chaotic system, and systems implementing feedback digital logic and algorithmic behavior
Substrate-Induced Self-Assembly of Cooperative Catalysts
Dissipative self-assembly processes in Nature rely on chemical fuels that activate proteins for assembly through the formation of a noncovalent complex. The catalytic activity of the assemblies causes fuel degradation, resulting in the formation of an assembly in a high-energy, out-of-equilibrium state. Herein, we apply this concept to a synthetic system and demonstrate that a substrate can induce the formation of vesicular assemblies, which act as cooperative catalysts for cleavage of the same substrate
Primitive selection of the fittest emerging through functional synergy in nucleopeptide networks
Many fundamental cellular and viral functions, including replication and translation, involve complex ensembles hosting synergistic activity between nucleic acids and proteins/peptides. There is ample evidence indicating that the chemical precursors of both nucleic acids and peptides could be efficiently formed in the prebiotic environment. Yet, studies on nonenzymatic replication, a central mechanism driving early chemical evolution, have focused largely on the activity of each class of these molecules separately. We show here that short nucleopeptide chimeras can replicate through autocatalytic and cross-catalytic processes, governed syn-ergistically by the hybridization of the nucleobase motifs and the assembly propensity of the peptide segments. Unequal assembly-dependent replication induces clear selectivity toward the formation of a certain species within small networks of complementary nucleopeptides. The selectivity pattern may be influenced and indeed maximized to the point of almost extinction of the weakest replicator when the system is studied far from equilibrium and manipulated through changes in the physical (flow) and chemical (template and inhibition) conditions. We postulate that similar processes may have led to the emergence of the first functional nucleic-acid-peptide assemblies prior to the origin of life. Furthermore, spontaneous formation of related replicating complexes could potentially mark the initiation point for information transfer and rapid progression in complexity within primitive environments, which would have facilitated the development of a variety of functions found in extant biological assembliesThe research was supported by the H2020 FET-Open (A.d.l.E and G.A.; CLASSY project, Grant Agreement Nº 862081), an NSF-BSF grant (GA; BSF-2015671), and the Spanish Ministry of Economy and Competitivity (A.d.l.E; MINECO: CTQ-2014-53673-P, CTQ-2017-89539-P, and EUIN2017-87022). The European COST Action CM1304 funded a Short-Term Scientific Mission of S.M.R. to BGU. A.K.B. received support from the BGU Kreitmann fellowships progra
Delocalized single-photon Dicke states and the Leggett- Garg inequality in solid state systems
We show how to realize a single-photon Dicke state in a large one-dimensional
array of two- level systems, and discuss how to test its quantum properties.
Realization of single-photon Dicke states relies on the cooperative nature of
the interaction between a field reservoir and an array of two-level-emitters.
The resulting dynamics of the delocalized state can display Rabi-like
oscillations when the number of two-level emitters exceeds several hundred. In
this case the large array of emitters is essentially behaving like a
mirror-less cavity. We outline how this might be realized using a
multiple-quantum-well structure and discuss how the quantum nature of these
oscillations could be tested with the Leggett-Garg inequality and its
extensions.Comment: 29 pages, 5 figures, journal pape
Required Levels of Catalysis for Emergence of Autocatalytic Sets in Models of Chemical Reaction Systems
The formation of a self-sustaining autocatalytic chemical network is a necessary but not sufficient condition for the origin of life. The question of whether such a network could form “by chance” within a sufficiently complex suite of molecules and reactions is one that we have investigated for a simple chemical reaction model based on polymer ligation and cleavage. In this paper, we extend this work in several further directions. In particular, we investigate in more detail the levels of catalysis required for a self-sustaining autocatalytic network to form. We study the size of chemical networks within which we might expect to find such an autocatalytic subset, and we extend the theoretical and computational analyses to models in which catalysis requires template matching
Stochastic Study of the Effect of Ionic Strength on Noncovalent Interactions in Protein Pores
Salt plays a critical role in the physiological activities of cells. We show that ionic strength significantly affects the kinetics of noncovalent interactions in protein channels, as observed in stochastic studies of the transfer of various analytes through pores of wild-type and mutant α-hemolysin proteins. As the ionic strength increased, the association rate constant of electrostatic interactions was accelerated, whereas those of both hydrophobic and aromatic interactions were retarded. Dramatic decreases in the dissociation rate constants, and thus increases in the overall reaction formation constants, were observed for all noncovalent interactions studied. The results suggest that with the increase of salt concentration, the streaming potentials for all the protein pores decrease, whereas the preferential selectivities of the pores for either cations or anions drop. Furthermore, results also show that the salt effect on the rate of association of analytes to a pore differs significantly depending on the nature of the noncovalent interactions occurring in the protein channel. In addition to providing new insights into the nature of analyte-protein pore interactions, the salt-dependence of noncovalent interactions in protein pores observed provides a useful means to greatly enhance the sensitivity of the nanopore, which may find useful application in stochastic sensing
Fabrication and characterization of vacuum deposited fluorescein thin films
Simple vacuum evaporation technique for deposition of dyes on various solid
surfaces has been developed. The method is compatible with conventional
solvent-free nanofabrication processing enabling fabrication of nanoscale
optoelectronic devices. Thin films of fluorescein were deposited on glass,
fluorine-tin-oxide (FTO) coated glass with and without atomically layer
deposited (ALD) nanocrystalline 20 nm thick anatase TiO2 coating. Surface
topology, absorption and emission spectra of the films depends on their
thickness and the material of supporting substrate. On a smooth glass surface
the dye initially formes islands before merging into a uniform layer after 5 to
10 monolayers. On FTO covered glass the absorption spectra are similar to
fluorescein solution in ethanol. Absorption spectra on ALD-TiO2 is red shifted
compared to the film deposited on bare FTO. The corresponding emission spectra
at {\lambda} = 458 nm excitation show various thickness and substrate dependent
features, while the emission of films deposited on TiO2 is quenched due to the
effective electron transfer to the semiconductor conduction band.Comment: 24 pages including 5 figure
Network Analysis of Biochemical Logic for Noise Reduction and Stability: A System of Three Coupled Enzymatic AND Gates
We develop an approach aimed at optimizing the parameters of a network of
biochemical logic gates for reduction of the "analog" noise buildup.
Experiments for three coupled enzymatic AND gates are reported, illustrating
our procedure. Specifically, starch - one of the controlled network inputs - is
converted to maltose by beta-amylase. With the use of phosphate (another
controlled input), maltose phosphorylase then produces glucose. Finally,
nicotinamide adenine dinucleotide (NAD+) - the third controlled input - is
reduced under the action of glucose dehydrogenase to yield the optically
detected signal. Network functioning is analyzed by varying selective inputs
and fitting standardized few-parameters "response-surface" functions assumed
for each gate. This allows a certain probe of the individual gate quality, but
primarily yields information on the relative contribution of the gates to noise
amplification. The derived information is then used to modify our experimental
system to put it in a regime of a less noisy operation.Comment: 31 pages, PD
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