Dynamic star formation in the massive DR21 filament

The formation of massive stars is a highly complex process in which it is not clear whether the star-forming gas is in global gravitational collapse or in an equilibrium state, supported by turbulence. By studying one of the most massive and dense star-forming regions in the Galaxy at a distance of less than 3 kpc, the filament containing the well-known sources DR21 and DR21(OH), we expect to find observational signatures that allow to discriminate between the two views. We use molecular line data from our 13CO 1-0, CS 2-1, and N2H+ 1-0 survey of the Cygnus X region obtained with the FCRAO and high-angular resolution observations of CO, CS, HCO+, N2H+, and H2CO, obtained with the IRAM 30m telescope. We observe a complex velocity field and velocity dispersion in the DR21 filament in which regions of highest column-density, i.e. dense cores, have a lower velocity dispersion than the surrounding gas and velocity gradients that are not (only) due to rotation. Infall signatures in optically thick line profiles of HCO+ and 12CO are observed along and across the whole DR21 filament. From modelling the observed spectra, we obtain a typical infall speed of 0.6 km/s and mass accretion rates of the order of a few 10^-3 Msun/yr for the two main clumps constituting the filament. These massive (4900 and 3300 Msun) clumps are both gravitationally contracting. All observed kinematic features in the DR21 filament can be explained if it is formed by the convergence of flows at large scales and is now in a state of global gravitational collapse. Whether this convergence of flows originated from self-gravity at larger scales or from other processes can not be settled with the present study. The observed velocity field and velocity dispersion are consistent with results from (magneto)-hydrodynamic simulations where the cores lie at the stagnation points of convergent turbulent flows.Comment: Astronomy and Astrophysics, in pres

Blood pressure–associated polymorphism controls ARHGAP42 expression via serum response factor DNA binding

We recently demonstrated that selective expression of the Rho GTPase-activating protein ARHGAP42 in smooth muscle cells (SMCs) controls blood pressure by inhibiting RhoA-dependent contractility, providing a mechanism for the blood pressure–associated locus within the ARHGAP42 gene. The goals of the current study were to identify polymorphisms that affect ARHGAP42 expression and to better assess ARHGAP42’s role in the development of hypertension. Using DNase I hypersensitivity methods and ENCODE data, we have identified a regulatory element encompassing the ARHGAP42 SNP rs604723 that exhibits strong SMC-selective, allele-specific activity. Importantly, CRISPR/Cas9–mediated deletion of this element in cultured human SMCs markedly reduced endogenous ARHGAP42 expression. DNA binding and transcription assays demonstrated that the minor T allele variation at rs604723 increased the activity of this fragment by promoting serum response transcription factor binding to a cryptic cis-element. ARHGAP42 expression was increased by cell stretch and sphingosine 1-phosphate in a RhoA-dependent manner, and deletion of ARHGAP42 enhanced the progression of hypertension in mice treated with DOCA-salt. Our analysis of a well-characterized cohort of untreated borderline hypertensive patients suggested that ARHGAP42 genotype has important implications in regard to hypertension risk. Taken together, our data add insight into the genetic mechanisms that control blood pressure and provide a potential target for individualized antihypertensive therapies

First Results from High Angular Resolution ALMA Observations Toward the HL Tau Region

We present Atacama Large Millimeter/submillimeter Array (ALMA) observations from the 2014 Long Baseline Campaign in dust continuum and spectral line emission from the HL Tau region. The continuum images at wavelengths of 2.9, 1.3, and 0.87 mm have unprecedented angular resolutions of 0.075 arcseconds (10 AU) to 0.025 arcseconds (3.5 AU), revealing an astonishing level of detail in the circumstellar disk surrounding the young solar analogue HL Tau, with a pattern of bright and dark rings observed at all wavelengths. By fitting ellipses to the most distinct rings, we measure precise values for the disk inclination (46.72pm0.05 degrees) and position angle (+138.02pm0.07 degrees). We obtain a high-fidelity image of the 1.0 mm spectral index ($\alpha$), which ranges from $\alpha\sim2.0$ in the optically-thick central peak and two brightest rings, increasing to 2.3-3.0 in the dark rings. The dark rings are not devoid of emission, we estimate a grain emissivity index of 0.8 for the innermost dark ring and lower for subsequent dark rings, consistent with some degree of grain growth and evolution. Additional clues that the rings arise from planet formation include an increase in their central offsets with radius and the presence of numerous orbital resonances. At a resolution of 35 AU, we resolve the molecular component of the disk in HCO+ (1-0) which exhibits a pattern over LSR velocities from 2-12 km/s consistent with Keplerian motion around a ~1.3 solar mass star, although complicated by absorption at low blue-shifted velocities. We also serendipitously detect and resolve the nearby protostars XZ Tau (A/B) and LkHa358 at 2.9 mm.Comment: 11 pages, 5 figures, 2 tables, accepted for publication in the Astrophysical Journal Letter

Kinetic temperature of massive star-forming molecular clumps measured with formaldehyde IV. The ALMA view of N113 and N159W in the LMC

We mapped the kinetic temperature structure of two massive star-forming regions, N113 and N159W, in the Large Magellanic Cloud (LMC). We have used ~1.′′6 (~0.4 pc) resolution measurements of the para-H2CO JKaKc = 303–202, 322–221, and 321–220 transitions near 218.5 GHz to constrain RADEX non local thermodynamic equilibrium models of the physical conditions. The gas kinetic temperatures derived from the para-H2CO line ratios 322–221/303–202 and 321–220/303–202 range from 28 to 105 K in N113 and 29 to 68 K in N159W. Distributions of the dense gas traced by para-H2CO agree with those of the 1.3 mm dust and Spitzer 8.0 μm emission, but they do not significantly correlate with the Hα emission. The high kinetic temperatures (Tkin ≳ 50 K) of the dense gas traced by para-H2CO appear to be correlated with the embedded infrared sources inside the clouds and/or young stellar objects in the N113 and N159W regions. The lower temperatures (Tkin < 50 K) were measured at the outskirts of the H2CO-bearing distributions of both N113 and N159W. It seems that the kinetic temperatures of the dense gas traced by para-H2CO are weakly affected by the external sources of the Hα emission. The non thermal velocity dispersions of para-H2CO are well correlated with the gas kinetic temperatures in the N113 region, implying that the higher kinetic temperature traced by para-H2CO is related to turbulence on a ~0.4 pc scale. The dense gas heating appears to be dominated by internal star formation activity, radiation, and/or turbulence. It seems that the mechanism heating the dense gas of the star-forming regions in the LMC is consistent with that in Galactic massive star-forming regions located in the Galactic plane

Probing Episodic Accretion in Very Low Luminosity Objects

Episodic accretion has been proposed as a solution to the long-standing luminosity problem in star formation; however, the process remains poorly understood. We present observations of line emission from N2H+ and CO isotopologues using the Atacama Large Millimeter/submillimeter Array (ALMA) in the envelopes of eight very low luminosity objects (VeLLOs). In five of the sources the spatial distribution of emission from N2H+ and CO isotopologues shows a clear anticorrelation. It is proposed that this is tracing the CO snow line in the envelopes: N2H+ emission is depleted toward the center of these sources, in contrast to the CO isotopologue emission, which exhibits a peak. The positions of the CO snow lines traced by the N2H+ emission are located at much larger radii than those calculated using the current luminosities of the central sources. This implies that these five sources have experienced a recent accretion burst because the CO snow line would have been pushed outward during the burst because of the increased luminosity of the central star. The N2H+ and CO isotopologue emission from DCE161, one of the other three sources, is most likely tracing a transition disk at a later evolutionary stage. Excluding DCE161, five out of seven sources (i.e., ~70%) show signatures of a recent accretion burst. This fraction is larger than that of the Class 0/I sources studied by Jørgensen et al. and Frimann et al., suggesting that the interval between accretion episodes in VeLLOs is shorter than that in Class 0/I sources

Mesenchymal cell survival in airway and interstitial pulmonary fibrosis

Fibrotic reactions in the airways of the lung or the pulmonary interstitium are a common pathologic outcome after exposure to a wide variety of toxic agents, including metals, particles or fibers. The survival of mesenchymal cells (fibroblasts and myofibroblasts) is a key factor in determining whether a fibroproliferative response that occurs after toxic injury to the lung will ultimately resolve or progress to a pathologic state. Several polypeptide growth factors, including members of the platelet-derived growth factor (PDGF) family and the epidermal growth factor (EGF) family, are prosurvival factors that stimulate a replicative and migratory mesenchymal cell phenotype during the early stages of lung fibrogenesis. This replicative phenotype can progress to a matrix synthetic phenotype in the presence of transforming growth factor-β1 (TGF-β1). The resolution of a fibrotic response requires growth arrest and apoptosis of mesenchymal cells, whereas progressive chronic fibrosis has been associated with mesenchymal cell resistance to apoptosis. Mesenchymal cell survival or apoptosis is further influenced by cytokines secreted during Th1 inflammation (e.g., IFN-γ) or Th2 inflammation (e.g., IL-13) that modulate the expression of growth factor activity through the STAT family of transcription factors. Understanding the mechanisms that regulate the survival or death of mesenchymal cells is central to ultimately developing therapeutic strategies for lung fibrosis

ATLASGAL - Ammonia observations towards the southern Galactic Plane

Context: The initial conditions of molecular clumps in which high-mass stars form are poorly understood. In particular, a more detailed study of the earliest evolutionary phases is needed. The APEX Telescope Large Area Survey of the whole inner Galactic disk at 870 μm, ATLASGAL, has therefore been conducted to discover high-mass star-forming regions at different evolutionary phases. Aims: We derive properties such as velocities, rotational temperatures, column densities, and abundances of a large sample of southern ATLASGAL clumps in the fourth quadrant. Methods: Using the Parkes telescope, we observed the NH3 (1, 1) to (3, 3) inversion transitions towards 354 dust clumps detected by ATLASGAL within a Galactic longitude range between 300° and 359° and a latitude within ± 1.5°. For a subsample of 289 sources, the N2H+ (1–0) line was measured with the Mopra telescope. Results: We measured a median NH3 (1, 1) line width of ~ 2 km s-1, rotational temperatures from 12 to 28 K with a mean of 18 K, and source-averaged NH3 abundances from 1.6 × 10-6 to 10-8. For a subsample with detected NH3 (2, 2) hyperfine components, we found that the commonly used method to compute the (2, 2) optical depth from the (1, 1) optical depth and the (2, 2) to (1, 1) main beam brightness temperature ratio leads to an underestimation of the rotational temperature and column density. A larger median virial parameter of ~ 1 is determined using the broader N2H+ line width than is estimated from the NH3 line width of ~ 0.5 with a general trend of a decreasing virial parameter with increasing gas mass. We obtain a rising NH3 (1, 1)/N2H+ line-width ratio with increasing rotational temperature. Conclusions: A comparison of NH3 line parameters of ATLASGAL clumps to cores in nearby molecular clouds reveals smaller velocity dispersions in low-mass than high-mass star-forming regions and a warmer surrounding of ATLASGAL clumps than the surrounding of low-mass cores. The NH3 (1, 1) inversion transition of 49% of the sources shows hyperfine structure anomalies. The intensity ratio of the outer hyperfine structure lines with a median of 1.27 ± 0.03 and a standard deviation of 0.45 is significantly higher than 1, while the intensity ratios of the inner satellites with a median of 0.9 ± 0.02 and standard deviation of 0.3 and the sum of the inner and outer hyperfine components with a median of 1.06 ± 0.02 and standard deviation of 0.37 are closer to 1

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

The 2014 ALMA Long Baseline Campaign: First Results from High Angular Resolution Observations toward the HL Tau Region

Interstellar matter and star formatio