Data Descriptor : A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins

Under ongoing climate change and increasing anthropogenic activity, which continuously challenge ecosystem resilience, an in-depth understanding of ecological processes is urgently needed. Lakes, as providers of numerous ecosystem services, face multiple stressors that threaten their functioning. Harmful cyanobacterial blooms are a persistent problem resulting from nutrient pollution and climate-change induced stressors, like poor transparency, increased water temperature and enhanced stratification. Consistency in data collection and analysis methods is necessary to achieve fully comparable datasets and for statistical validity, avoiding issues linked to disparate data sources. The European Multi Lake Survey (EMLS) in summer 2015 was an initiative among scientists from 27 countries to collect and analyse lake physical, chemical and biological variables in a fully standardized manner. This database includes in-situ lake variables along with nutrient, pigment and cyanotoxin data of 369 lakes in Europe, which were centrally analysed in dedicated laboratories. Publishing the EMLS methods and dataset might inspire similar initiatives to study across large geographic areas that will contribute to better understanding lake responses in a changing environment.Peer reviewe

Stratification strength and light climate explain variation in chlorophyll a at the continental scale in a European multilake survey in a heatwave summer

To determine the drivers of phytoplankton biomass, we collected standardized morphometric, physical, and biological data in 230 lakes across the Mediterranean, Continental, and Boreal climatic zones of the European continent. Multilinear regression models tested on this snapshot of mostly eutrophic lakes (median total phosphorus [TP] = 0.06 and total nitrogen [TN] = 0.7 mg L−1), and its subsets (2 depth types and 3 climatic zones), show that light climate and stratification strength were the most significant explanatory variables for chlorophyll a (Chl a) variance. TN was a significant predictor for phytoplankton biomass for shallow and continental lakes, while TP never appeared as an explanatory variable, suggesting that under high TP, light, which partially controls stratification strength, becomes limiting for phytoplankton development. Mediterranean lakes were the warmest yet most weakly stratified and had significantly less Chl a than Boreal lakes, where the temperature anomaly from the long-term average, during a summer heatwave was the highest (+4°C) and showed a significant, exponential relationship with stratification strength. This European survey represents a summer snapshot of phytoplankton biomass and its drivers, and lends support that light and stratification metrics, which are both affected by climate change, are better predictors for phytoplankton biomass in nutrient-rich lakes than nutrient concentrations and surface temperature

Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues

Background Gene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly important in biological research due to the high-throughput and accuracy of qRT-PCR. For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes or internal control genes is required. The stability of reference genes has a tremendous effect on the results of relative quantification of gene expression by qRT-PCR. The expression stability of reference genes could vary according to tissues, age of individuals and experimental conditions. In the pig however, very little information is available on the expression stability of reference genes. The aim of this research was therefore to develop a new set of reference genes which can be used for normalization of mRNA expression data of genes expressed in varieties of porcine tissues at different ages. Results The mRNA expression stability of nine commonly used reference genes (B2M, BLM, GAPDH, HPRT1, PPIA, RPL4, SDHA, TBP and YWHAZ) was determined in varieties of tissues collected from newborn, young and adult pigs. geNorm, NormFinder and BestKeeper software were used to rank the genes according to their stability. geNorm software revealed that RPL4, PPIA and YWHAZ showed high stability in newborn and adult pigs, while B2M, YWHAZ and SDHA showed high stability in young pigs. In all cases, GAPDH showed the least stability in geNorm. NormFinder revealed that TBP was the most stable gene in newborn and young pigs, while PPIA was most stable in adult pigs. Moreover, geNorm software suggested that the geometric mean of three most stable gene would be the suitable combination for accurate normalization of gene expression study. Conclusions Although, there was discrepancy in the ranking order of reference genes obtained by different analysing software methods, the geometric mean of the RPL4, PPIA and YWHAZ seems to be the most appropriate combination of housekeeping genes for accurate normalization of gene expression data in different porcine tissues at different ages

Development of obsessive and compulsive behaviors scale of mothers in postpartum period regarding baby care: Validity and reliability

PMID = 3161759

Effect of dietary mannan oligosaccharide with or without oregano essential oil and hop extract supplementation on the performance and slaughter characteristics of male broilers

The effects of some alternative feed additives for antibiotic growth promoters on performance and some slaughter characteristics were examined in broilers fed wheat-soya based basal starter and finisher diets. A total of 2160 one-day-old male broiler chicks were randomly allocated to six groups with six replicate pens per treatment. The treatments were the basal diet (Control), and the basal diet supplemented with an antibiotic growth promoter (AGP); a prebiotic, mannan oligosaccharide (Bio-Mos, MOS); an essential oil of oregano (Herb-Mos Oregano, HMO); a plant extract of hop (Herb-Mos Hops, HMH) or a mixture of Herb-Mos Oregano and Herb-Mos Hops (HMOH). There were significant effects of dietary treatments on body weight and feed conversion ratio in the 0 - 21 d period, and on body weight and feed consumption in the 0 - 42 d period. The addition of all experimental additives to the diet resulted in significantly higher body weights as compared to the control treatment at both 21 and 42 days of age. Feed intakes were significantly different between the treatments at 0 - 21 d and 22 - 42 d periods, but not during the 0 - 42 d period. However, during the 0 - 21 d period the broiler chickens that received diets supplemented with AGP, MOS, HMO, HMH and HMOH had significantly better feed conversion ratios than the control group, but this pattern was not sustained during the finisher period (22 - 42 d). Mortality rate, hot carcass yield and relative weights of the small intestines, pancreas, abdominal fat and bursa of fabricius were not affected by experimental treatments. The HMH supplementation increased relative liver weight. These results showed that AGP, MOS and herbal feed additive (HMO, HMH, HMOH) supplementation to a diet provided significant advantages on broiler growth performance through a 42-d growth period. However, the combined supplementation of HMO and HMH did not exert either synergistic or additive benefits on the live performance of the broilers. These results also proved that MOS, HMO, HMH and HMOH improved broiler live performance as well as an AGP in both the starter and through the grower period. Furthermore, outstanding advantages were evidenced for the HMH treatment in particular. Therefore, the MOS, HMO, HMH and HMOH performance enhancer feed additives of natural origin may be considered as potential substitutes for AGP in broiler diets

The multidimensional nature of food neophobia

People vary in their willingness to try new foods. This variation, which is most frequently measured using the Food Neophobia Scale (FNS; Pliner & Hobden, 1992), has been interpreted as unidimensional. In four studies (N's = 210, 306, 160, and 161), we 1) demonstrate that food neophobia varies across meat and plant dimensions, 2) explore the validity of a measure of meat and plant neophobia, and 3) test whether these food neophobia dimensions predict decisions to eat a novel food item (i.e., a snack bar that contains insects). Mixed-effects model across the four studies indicated that the two dimensions differentially relate to a number of variables, including disgust sensitivity, animal empathy, and masculinity. Women scored higher on meat neophobia than men, but the sexes did not differ on plant neophobia. Only meat neophobia uniquely predicted eating a novel insect-based snack bar. Overall, these results extend knowledge regarding orientations toward novel foods

The effect of single or combined dietary supplementation of prebiotics, organic acid and probiotics on performance and slaughter characteristics of broilers

A study was conducted to investigate the effect of dietary supplementation of an organic acid, a probiotic or a prebiotic alone or the prebiotic combined with the organic acid or the probiotic on the performance and slaughter characteristics of broiler chickens fed a maize-soya based diet. The six dietary treatments were: a basal diet (negative control) and diets containing 0.5 g mannan oligosaccharide/kg (prebiotic), or 1.0 g formic acid/kg (organic acid), or a probiotic at 0.5 g/kg, or 0.5 g prebiotic/kg + 1.0 g organic acid/kg, or 0.5 g prebiotic/kg + 0.5 g probiotic/kg feed. Each treatment consisted of eight pens with 50 birds per pen (25 male + 25 female). All dietary supplements, alone and in combination improved live weight significantly at both 21 and 42 days of age compared with the control. However, combinations of the prebiotic with either the organic acid or the probiotic had no additive benefit at 21 and 42 days of age in comparison with the prebiotic alone. The feed intake of the birds was significantly increased with prebiotic supplementation at day 21, but not at day 42. Organic acid significantly improved feed conversion ratio at day 21. The combination of prebiotic and probiotic significantly improved the feed conversion ratio at both 21 and 42 days in comparison with the control. At days 21 and 42 bird mortality was significantly higher in the treatments containing organic acid and organic acid with the prebiotic. In the female birds no slaughter traits were affected by dietary treatments. However, liver weight as a percentage of live weight in the male birds was significantly lowered with prebiotic and probiotic supplementation. Prebiotic supplementation with organic acid resulted in a significantly lower weight of the small intestines compared with the control. In general, the different feed additive regimens that include the prebiotic, probiotic, organic acid, prebiotic with organic acid and prebiotic with probiotic improved the growth rate of the birds significantly compared to the control treatment. The significant improvement in feed conversion ratio when the prebiotic and probiotic were supplemented together suggests a synergism between them