Ligand-dependent Inhibition of CD1d-restricted NKT Cell Development in Mice Transgenic for the Activating Receptor Ly49D

In addition to their CD1d-restricted T cell receptor (TCR), natural killer T (NKT) cells express various receptors normally associated with NK cells thought to act, in part, as modulators of TCR signaling. Immunoreceptor-tyrosine activation (ITAM) and inhibition (ITIM) motifs associated with NK receptors may augment or attenuate perceived TCR signals respectively, potentially influencing NKT cell development and function. ITIM-containing Ly49 family receptors expressed by NKT cells are proposed to play a role in their development and function. We have produced mice transgenic for the ITAM-associated Ly49D and ITIM-containing Ly49A receptors and their common ligand H2-Dd to determine the importance of these signaling interplays in NKT cell development. Ly49D/H2-Dd transgenic mice had selectively and severely reduced numbers of thymic and peripheral NKT cells, whereas both ligand and Ly49D transgenics had normal numbers of NKT cells. CD1d tetramer staining revealed a blockade of NKT cell development at an early precursor stage. Coexpression of a Ly49A transgene partially rescued NKT cell development in Ly49D/H2-Dd transgenics, presumably due to attenuation of ITAM signaling. Thus, Ly49D-induced ITAM signaling is incompatible with the early development of cells expressing semi-invariant CD1d-restricted TCRs and appropriately harmonized ITIM–ITAM signaling is likely to play an important role in the developmental program of NKT cells

Permafrost Thaw and Liberation of Inorganic Nitrogen in Eastern Siberia

The currently observed climate warming will lead to widespread degradation of near-surface permafrost, which may release substantial amounts of inorganic nitrogen (N) into arctic ecosystems. We studied 11 soil profiles at three different sites in arctic eastern Siberia to assess the amount of inorganic N stored in arctic permafrost soils. We modelled the potential thickening of the active layer for these sites using the CryoGrid2 permafrost model and representative concentration pathways (RCPs) 4.5 (a stabilisation scenario) and 8.5 (a business as usual emission scenario, with increasing carbon emissions). The modelled increases in active-layer thickness (ALT) were used to estimate potential annual liberation of inorganic N from permafrost soils during the course of climate change. We observed significant stores of inorganic ammonium in permafrost, up to 40-fold higher than in the active layer. The modelled increase in ALT under the RCP8.5 scenario can result in substantial liberation of N, reaching values up to the order of magnitude of annual fixation of atmospheric N in arctic soils. However, the thaw-induced liberation of N represents only a small flux in comparison with the overall ecosystem N cycling

Simulation assisted process chain design for the manufacturing of bulk hybrid shafts with tailored properties

To manufacture semi-finished hybrid workpieces with tailored properties, a finite element simulation assisted process chain design was investigated. This includes the process steps of cross wedge rolling, hot geometry inspection, induction hardening, and fatigue testing. The process chain allows the utilisation of material combinations such as high-strength steels with low-cost and easy to process steels. Here, plasma transferred arc welding is applied to supply the process chain with hybrid specimen featuring different steel grades. An overview of the numerical approaches to consider the various physical phenomena in each of the process steps is presented. The properties of the component behaviour were investigated via the finite element method (FEM) and theoretical approaches. At first, the manufacturing of a hybrid workpiece featuring a near net shape geometry with improved mechanical properties due to recrystallising the weld was computed, using the example of a cross wedge rolling process. The rolling process was designed by means of FEM to determine suitable process parameters and to reduce experimental testing. An optical multi-scale geometry inspection of the hot workpiece is meant to be carried out after each manufacturing step to detect potential undesired forming or cooling-induced deformations. Due to the heat transfer from the hot component to the ambient medium, an optical measurement is affected by the developing inhomogeneous refractive index field in air. To gain a basic understanding of the refractive index field and induced light deflection effects, computations were conducted using heat transfer and ray tracing simulations. According to the proposed process route, a subsequent local heat treatment of the hybrid component is required to adapt the mechanical properties by a spray cooling assisted induction hardening. The heat treatment step was computed via a 2D FEM calculation. After finishing by machining, the hybrid material shafts are examined in fatigue tests under load conditions. To predict the component’s lifetime under rolling contact fatigue, a damage accumulation model was combined with an FE simulation. The resulting residual stress state after quenching and the geometry after the finishing process were used as input data for the fatigue life calculations

The nitrogen stock of the ice-rich yedoma domain

Recent studies on permafrost organic matter (OM) suggest that a portion of previously frozen carbon will enter the active carbon cycle as high latitudes warm. Less is known about the fate of other OM components, including nutrients such as nitrogen (N). The abundance and availability of N following permafrost thaw will regulate the ability of plants to offset carbon losses. Additionally, lateral N losses could alter aquatic food webs. There is growing evidence that some N is lost vertically as N2O, a greenhouse gas 300 times stronger than CO2 over 100 years. Despite broad recognition of its role regulating both carbon and non-carbon aspects of the permafrost climate feedback, estimates of permafrost N remain uncertain. To address this knowledge gap, we quantified N content for different stratigraphic units, including yedoma, Holocene cover deposits, refrozen thermokarst deposits, taberal sediments, and active layer soils. The resulting N estimates from this one permafrost region were similar in magnitude to previous estimates for the entire permafrost zone. We conclude that the permafrost N pool is much larger than currently appreciated and a substantial pool of permafrost N could be mobilized after thaw, with continental-scale consequences for biogeochemical budgets and global-scale consequences

Permafrost thaw and release of inorganic nitrogen from polygonal tundra soils in eastern Siberia

The currently observed climate warming will lead to substantial permafrost degradation and mobilization of formerly freeze-locked matter. Based on recent findings, we assume that there are substantial stocks of inorganic nitrogen (N) within the perennially frozen ground of arctic ecosystems. We studied eleven soil profiles down to one meter depth below surface at three different sites in arctic eastern Siberia, covering polygonal tundra and river floodplains, to assess the amount of inorganic N stores in arctic permafrost-affected soils. Furthermore, we modeled the potential thickening of the seasonally unfrozen uppermost soil (active) layer for these sites, using the CryoGrid2 permafrost model and representation concentration pathway (RCP) 4.5 and 8.5 scenarios. The first scenario, RCP4.5, is a stabilization pathway that reaches plateau atmospheric carbon concentrations early in the 21st century; the second, RCP8.5, is a business as usual emission scenario with increasing carbon emissions. The modeled increases in active layer thickness (ALT) were used to estimate potential annual N mobilization from permafrost-affected soils in the course of climate-induced active-layer deepening. We observed significant stores of inorganic ammonium in the perennially frozen ground of all investigated soils, up to 40-fold higher than in the active layer. The modeled ALT increase until 2100 under the RCP8.5 scenario was between 19 ± 3 cm and 35 ± 6 cm, depending on the location. Under the RCP4.5 scenario, the ALT remained stable in all investigated soils. Our estimated mean annual N release under the RCP8.5 scenario is between 8 ± 3 mg m−2 and 81 ± 14 mg m−2 for the different locations, which reaches values up to the order of magnitude of annual fixation of atmospheric N in arctic soils. However, the thawing induced release of N represents only a small flux in comparison with the overall ecosystem N cycling

Miteinander reden

Landnutzungskonflikte entwickeln sich typischerweise zwischen verschiedenen Nutzergruppen. Klassische Konfliktlinien verlaufen zum Beispiel zwischen Landwirtschaft und Naturschutz, zwischen Tourismus und Energiewende, zwischen Siedlungsdruck und Erholungsraum. Und nicht selten sind mehrere dieser Bereiche und ihre Ansprüche an Land und Landschaft involviert. Die klassischen Konfliktlinien haben über die Jahre zu einem – zugespitzt formuliert – routinierten Schlagabtausch mit eingeübten Argumenten zwischen bekannten Gegnerinnen geführt. Häufig genug reden sie gar nicht miteinander – man kennt ja die Positionen und Argumente. Eine Situation, die die Lösung von Landnutzungskonflikten verlässlich verhinder

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements