C/EBPβ regulates homeostatic and oncogenic gastric cell proliferation

Cancer of the stomach is among the leading causes of death from cancer worldwide. The transcription factor C/EBPβ is frequently overexpressed in gastric cancer and associated with the suppression of the differentiation marker TFF1. We show that the murine C/EBP{beta} knockout stomach displays unbalanced homeostasis and reduced cell proliferation and that tumorigenesis of human gastric cancer xenograft is inhibited by knockdown of C/EBPβ. Cross-species comparison of gene expression profiles between C/EBPβ-deficient murine stomach and human gastric cancer revealed a subset of tumors with a C/EBPβ signature. Within this signature, the RUNX1t1 tumor suppressor transcript was down-regulated in 38% of gastric tumor samples. The RUNX1t1 promoter was frequently hypermethylated and ectopic expression of RUNX1t1 in gastric cancer cells inhibited proliferation and enhanced TFF1 expression. These data suggest that the tumor suppressor activity of both RUNX1t1 and TFF1 are mechanistically connected to C/EBPβ and that cross-regulation between C/EBPβ-RUNX1t1-TFF1 plays an important role in gastric carcinogenesis

COMPOSIÇÃO CORPORAL DE MACROELEMENTOS MINERAIS EM BOVINOS E BUBALINOS EM CONFINAMENTO

A pesquisa foi conduzida na Universidade Federal de Viçosa(MG), no período entre dezembro de 1994 e julho de 1995 Objetivou-se com a pesquisa estimar o conteúdo corporal de macroelementos minerais de bovinos Nelore, mestiços europeu-zebu e bubalinos, abatidos em diferentes estádios de maturidade. Foram utilizados 35 bovinos machos não-castrados sendo 12 Nelore (NEL), 12 F1 Holandês-Nelore (HN), 11 bimestiços 1/4 Fleckvieh-5/16 Angus-7/16 Nelore (BM) e 11 bubalinos mestiços Mediterrâneo (BUF), com pesos vivos médios iniciais de 294; 404; 358; e 353 Kg, respectivamente, e idade média de 24 meses. Os animais de cada grupo genético foram, aleatoriamente, divididos em três grupos experimentais, abate inicial ou referência (AB) e dois grupos de alimentação ad libitum (AL1 e AL2). No grupo AB foram alocados quatro animais NEL, quatro HN, três BM e três BUF e nos grupos AL1 e AL2 quatro animais, de cada grupo genético. Após o período de adaptação de 60 dias, onde os animais receberam o mesmo tratamento, aqueles do grupo AB foram abatidos. Os animais dos grupos (AL1 e AL2) eram abatidos ao atingirem pesos equivalentes a 100 e 110 % do peso à maturidade da vaca do grupo racial correspondente. Estes pesos eram, respectivamente, 450 e 500 Kg para NEL e BUF e 500 e 550 Kg para HN e BM. A ração continha milho desintegrado com palha e sabugo, farelo de soja, uréia, minerais e feno de braquiária decumbens, numa proporção de concentrado:volumoso 50:50 e 13% de proteína bruta, na matéria seca. Após o abate, utilizando-se amostras representativas da carcaça e dos diferentes órgãos e tecidos corporais, foram determinadas as quantidades do macroelementos minerais (cálcio, fósforo, magnésio, sódio e potássio) contidas no corpo vazio dos animais. Para se estimar as mudanças na composição corporal nos intervalos de pesos incluídos na pesquisa, foram ajustadas equações de regressão dos logarítmos dos conteúdos de macroelementos minerais (Kg), em função do peso corporal vazio (PCV), em Kg. Verificou-se efeito (

Measurement of the polarisation of W bosons produced with large transverse momentum in pp collisions at sqrt(s) = 7 TeV with the ATLAS experiment

This paper describes an analysis of the angular distribution of W->enu and W->munu decays, using data from pp collisions at sqrt(s) = 7 TeV recorded with the ATLAS detector at the LHC in 2010, corresponding to an integrated luminosity of about 35 pb^-1. Using the decay lepton transverse momentum and the missing transverse energy, the W decay angular distribution projected onto the transverse plane is obtained and analysed in terms of helicity fractions f0, fL and fR over two ranges of W transverse momentum (ptw): 35 < ptw < 50 GeV and ptw > 50 GeV. Good agreement is found with theoretical predictions. For ptw > 50 GeV, the values of f0 and fL-fR, averaged over charge and lepton flavour, are measured to be : f0 = 0.127 +/- 0.030 +/- 0.108 and fL-fR = 0.252 +/- 0.017 +/- 0.030, where the first uncertainties are statistical, and the second include all systematic effects.Comment: 19 pages plus author list (34 pages total), 9 figures, 11 tables, revised author list, matches European Journal of Physics C versio

Observation of a new chi_b state in radiative transitions to Upsilon(1S) and Upsilon(2S) at ATLAS

The chi_b(nP) quarkonium states are produced in proton-proton collisions at the Large Hadron Collider (LHC) at sqrt(s) = 7 TeV and recorded by the ATLAS detector. Using a data sample corresponding to an integrated luminosity of 4.4 fb^-1, these states are reconstructed through their radiative decays to Upsilon(1S,2S) with Upsilon->mu+mu-. In addition to the mass peaks corresponding to the decay modes chi_b(1P,2P)->Upsilon(1S)gamma, a new structure centered at a mass of 10.530+/-0.005 (stat.)+/-0.009 (syst.) GeV is also observed, in both the Upsilon(1S)gamma and Upsilon(2S)gamma decay modes. This is interpreted as the chi_b(3P) system.Comment: 5 pages plus author list (18 pages total), 2 figures, 1 table, corrected author list, matches final version in Physical Review Letter