35 research outputs found
The microbiotest battery as an important component in the assessment of snowmelt toxicity in urban watercourses—preliminary studies
The aim of the study was to use a battery of
biotests composed of producers (Selenastrum
capricornutum, Sorghum saccharatum, Lepidium
sativum, and Sinapis alba), consumers (Thamnocephalus
platyurus), and decomposers (Tetrahymena thermophila)
to evaluate the toxicity of snowmelt and winter storm
water samples. The toxicity of the samples collected in
the winter period December to February (2010–2011), in
one of the largest agglomerations in Poland, the city of
Lodz, was compared to that of storm water samples taken
under similar conditions in June. The most toxic snowmelt
samples were found to be high acute hazard (class IV),
while the remaining samples were rated as slight acute
hazard (class II). L. sativum (in the Phytotox test) was the
most sensitive test organism, giving 27 % of all toxic
responses, followed by S. capricornutum with 23 % of
all responses. T. thermophila was the least sensitive, with
only 2 % of all toxic responses. The greatest range of
toxicity was demonstrated by samples from the single
family house catchment: no acute hazard (class I) to high
acute hazard (class IV
Bacteria homologus to Aeromonas capable of microcystin degradation
Water blooms dominated by cyanobacteria
are capable of producing hepatotoxins known as
microcystins. These toxins are dangerous to people and
to the environment. Therefore, for a better understanding
of the biological termination of this increasingly
common phenomenon, bacteria with the potential to
degrade cyanobacteria-derived hepatotoxins and the
degradative activity of culturable bacteria were studied.
Based on the presence of the mlrA gene, bacteria with a
homology to the Sphingopyxis and Stenotrophomonas
genera were identified as those presenting potential for
microcystins degradation directly in the water samples
from the Sulejów Reservoir (SU, Central Poland). However,
this biodegrading potential has not been confirmed in in
vitro experiments. The degrading activity of the culturable
isolates from the water studied was determined in more
than 30 bacterial mixes. An analysis of the biodegradation
of the microcystin-LR (MC-LR) together with an analysis of
the phylogenetic affiliation of bacteria demonstrated for
the first time that bacteria homologous to the Aeromonas
genus were able to degrade the mentioned hepatotoxin,
although the mlrA gene was not amplified. The maximal
removal efficiency of MC-LR was 48%. This study
demonstrates a new aspect of interactions between the
microcystin-containing cyanobacteria and bacteria from
the Aeromonas genus.The authors would like to
acknowledge the European Cooperation in Science
and Technology, COST Action ES 1105 “CYANOCOST -
Cyanobacterial blooms and toxins in water resources:
Occurrence, impacts and management” for adding value
to this study through networking and knowledge sharing
with European experts and researchers in the field. The
Sulejów Reservoir is a part of the Polish National Long-
Term Ecosystem Research Network and the European
LTER site
The microbiotest battery as an important component in the assessment of snowmelt toxicity in urban watercourses—preliminary studies
Toxic cyanobacteria in reservoirs in northeastern Brazil: detection using a molecular method
Aphanizomenon gracile (Nostocales), a cylindrospermopsin-producing cyanobacterium in Polish lakes
Data Descriptor : A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins
Under ongoing climate change and increasing anthropogenic activity, which continuously challenge ecosystem resilience, an in-depth understanding of ecological processes is urgently needed. Lakes, as providers of numerous ecosystem services, face multiple stressors that threaten their functioning. Harmful cyanobacterial blooms are a persistent problem resulting from nutrient pollution and climate-change induced stressors, like poor transparency, increased water temperature and enhanced stratification. Consistency in data collection and analysis methods is necessary to achieve fully comparable datasets and for statistical validity, avoiding issues linked to disparate data sources. The European Multi Lake Survey (EMLS) in summer 2015 was an initiative among scientists from 27 countries to collect and analyse lake physical, chemical and biological variables in a fully standardized manner. This database includes in-situ lake variables along with nutrient, pigment and cyanotoxin data of 369 lakes in Europe, which were centrally analysed in dedicated laboratories. Publishing the EMLS methods and dataset might inspire similar initiatives to study across large geographic areas that will contribute to better understanding lake responses in a changing environment.Peer reviewe
A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins
Assessment of heavy metals mobility and toxicity in contaminated sediments by sequential extraction and a battery of bioassays
Stratification strength and light climate explain variation in chlorophyll a at the continental scale in a European multilake survey in a heatwave summer
To determine the drivers of phytoplankton biomass, we collected standardized morphometric, physical, and biological data in 230 lakes across the Mediterranean, Continental, and Boreal climatic zones of the European continent. Multilinear regression models tested on this snapshot of mostly eutrophic lakes (median total phosphorus [TP] = 0.06 and total nitrogen [TN] = 0.7 mg L−1), and its subsets (2 depth types and 3 climatic zones), show that light climate and stratification strength were the most significant explanatory variables for chlorophyll a (Chl a) variance. TN was a significant predictor for phytoplankton biomass for shallow and continental lakes, while TP never appeared as an explanatory variable, suggesting that under high TP, light, which partially controls stratification strength, becomes limiting for phytoplankton development. Mediterranean lakes were the warmest yet most weakly stratified and had significantly less Chl a than Boreal lakes, where the temperature anomaly from the long-term average, during a summer heatwave was the highest (+4°C) and showed a significant, exponential relationship with stratification strength. This European survey represents a summer snapshot of phytoplankton biomass and its drivers, and lends support that light and stratification metrics, which are both affected by climate change, are better predictors for phytoplankton biomass in nutrient-rich lakes than nutrient concentrations and surface temperature