Impact of Visitation with Incarcerated Fathers on Behavioral Adjustment Among Children in the Foster Care System

This study sought to examine whether in-person visitation with incarcerated fathers related to less behavioral problems among children in foster care. The sample consisted of 282 youth (M = 10.18, SD = 2.36 years). Data were collected from the Illinois Department of Children and Family Services. Hierarchical Generalized Linear Modeling revealed paternal incarceration was associated with increased externalizing slope trajectories (ß1515 = .18, p = .025), but not internalizing. African American youth had lower externalizing slope trajectories compared to the remainder of the sample (ß20 = -.14, p = .032). The association between paternal incarceration and externalizing was attenuated among youth who visited fathers (ß5 = -.17, p = .008). Findings suggest paternal incarceration is associated with externalizing behaviors among youth in foster care, and visitation may be protective. In addition, African American youth appear more resilient in the face of paternal incarceration compared to youth of other backgrounds

Comparing Emotion Recognition Skills among Children with and without Jailed Parents

Approximately five million children in the United States have experienced a co-resident parent’s incarceration in jail or prison. Parental incarceration is associated with multiple risk factors for maladjustment, which may contribute to the increased likelihood of behavioral problems in this population. Few studies have examined early predictors of maladjustment among children with incarcerated parents, limiting scholars’ understanding about potential points for prevention and intervention. Emotion recognition skills may play a role in the development of maladjustment and may be amenable to intervention. The current study examined whether emotion recognition skills differed between three- to eight-year-old children with and without jailed parents. We hypothesized that children with jailed parents would have a negative bias in processing emotions and less accuracy compared to children without incarcerated parents. Data were drawn from 128 families, including 75 children (53.3% male, M = 5.37 years) with jailed parents and 53 children (39.6% male, M = 5.02 years) without jailed parents. Caregivers in both samples provided demographic information. Children performed an emotion recognition task in which they were asked to produce a label for photos expressing six different emotions (i.e., happy, surprised, neutral, sad, angry, fearful). For scoring, the number of positive and negative labels were totaled; the number of negative labels provided for neutral and positive stimuli were totaled (measuring negative bias/overextension of negative labels); and valence accuracy (i.e., positive, negative, neutral) and label accuracy were calculated. Results indicated a main effect of parental incarceration on the number of positive labels provided; children with jailed parents presented significantly fewer positive emotions than the comparison group. There was also a main effect of parental incarceration on negative bias (the overextension of negative labels); children with jailed parents had a negative bias compared to children without jailed parents. However, these findings did not hold when controlling for child age, race/ethnicity, receipt of special education services, and caregiver education. The results provide some evidence for the effect of the context of parental incarceration in the development of negative emotion recognition biases. Limitations and implications for future research and interventions are discussed

MYB10 and MYB72 are Required for Growth under Iron-Limiting Conditions

Iron is essential for photosynthesis and is often a limiting nutrient for plant productivity. Plants respond to conditions of iron deficiency by increasing transcript abundance of key genes involved in iron homeostasis, but only a few regulators of these genes have been identified. Using genome-wide expression analysis, we searched for transcription factors that are induced within 24 hours after transferring plants to iron-deficient growth conditions. Out of nearly 100 transcription factors shown to be up-regulated, we identified MYB10 and MYB72 as the most highly induced transcription factors. Here, we show that MYB10 and MYB72 are functionally redundant and are required for plant survival in alkaline soil where iron availability is greatly restricted. myb10myb72 double mutants fail to induce transcript accumulation of the nicotianamine synthase gene NAS4. Both myb10myb72 mutants and nas4-1 mutants have reduced iron concentrations, chlorophyll levels, and shoot mass under iron-limiting conditions, indicating that these genes are essential for proper plant growth. The double myb10myb72 mutant also showed nickel and zinc sensitivity, similar to the nas4 mutant. Ectopic expression of NAS4 rescues myb10myb72 plants, suggesting that loss of NAS4 is the primary defect in these plants and emphasizes the importance of nicotianamine, an iron chelator, in iron homeostasis. Overall, our results provide evidence that MYB10 and MYB72 act early in the iron-deficiency regulatory cascade to drive gene expression of NAS4 and are essential for plant survival under iron deficiency

BRUTUS and its paralogs, BTS LIKE1 and BTS LIKE2, encode important negative regulators of the iron deficiency response in Arabidopsis thaliana

Iron (Fe) is required for plant health, but it can also be toxic when present in excess. Therefore, Fe levels must be tightly controlled. The Arabidopsis thaliana E3 ligase BRUTUS (BTS) is involved in the negative regulation of the Fe deficiency response and we show here that the two A. thaliana BTS paralogs, BTS LIKE1 (BTSL1) and BTS LIKE2 (BTSL2) encode proteins that act redundantly as negative regulators of the Fe deficiency response. Loss of both of these E3 ligases enhances tolerance to Fe deficiency. We further generated a triple mutant with loss of both BTS paralogs and a partial loss of BTS expression that exhibits even greater tolerance to Fe deficient conditions and increased Fe accumulation without any resulting Fe toxicity effects. Finally, we identified a mutant carrying a novel missense mutation of BTS that exhibits an Fe deficiency response in the root when grown under both Fe-deficient and Fe-sufficient conditions, leading to Fe toxicity when plants are grown under Fe-sufficient conditions

Hemerythrin E3 ubiquitin ligases as negative regulators of iron homeostasis in plants

Iron (Fe) is an essential nutrient for plants, but at the same time its redox properties can make it a dangerous toxin inside living cells. Homeostasis between uptake, use and storage of Fe must be maintained at all times. A small family of unique hemerythrin E3 ubiquitin ligases found in green algae and plants play an important role in avoiding toxic Fe overload, acting as negative regulators of Fe homeostasis. Protein interaction data showed that they target specific transcription factors for degradation by the 26S proteasome. It is thought that the activity of the E3 ubiquitin ligases is controlled by Fe binding to the N-terminal hemerythrin motifs. Here we discuss what we have learned so far from studies on the HRZ (Hemerythrin RING Zinc finger) proteins in rice, the homologous BTS (BRUTUS) and root-specific BTSL (BRUTUS-LIKE) in Arabidopsis. A mechanistic model is proposed to help focus future research questions towards a full understanding of the regulatory role of these proteins in Fe homeostasis in plants

Elemental profiling of rice FOX lines leads to characterization of a new Zn plasma membrane transporter, OsZIP7

Iron (Fe) and zinc (Zn) are essential micronutrients required for proper development in both humans and plants. Rice (Oryza sativa L.) grains are the staple food for nearly half of the world’s population, but a poor source of metals such as Fe and Zn. Populations that rely on milled cereals are especially prone to Fe and Zn deficiencies, the most prevalent nutritional deficiencies in humans. Biofortification is a cost-effective solution for improvement of the nutritional quality of crops. However, a better understanding of the mechanisms underlying grain accumulation of mineral nutrients is required before this approach can achieve its full potential. Characterization of gene function is more time-consuming in crops than in model species such as Arabidopsis thaliana. Aiming to more quickly characterize rice genes related to metal homeostasis, we applied the concept of high throughput elemental profiling (ionomics) to Arabidopsis lines heterologously expressing rice cDNAs driven by the 35S promoter, named FOX (Full Length Over-eXpressor) lines. We screened lines expressing candidate genes that could be used in the development of biofortified grain. Among the most promising candidates, we identified two lines ovexpressing the metal cation transporter OsZIP7. OsZIP7 expression in Arabidopsis resulted in a 25% increase in shoot Zn concentrations compared to non-transformed plants. We further characterized OsZIP7 and showed that it is localized to the plasma membrane and is able to complement Zn transport defective (but not Fe defective) yeast mutants. Interestingly, we showed that OsZIP7 does not transport Cd, which is commonly transported by ZIP proteins. Importantly, OsZIP7-expressing lines have increased Zn concentrations in their seeds. Our results indicate that OsZIP7 is a good candidate for developing Zn biofortified rice. Moreover, we showed the use of heterologous expression of genes from crops in A. thaliana as a fast method for characterization of crop genes related to the ionome and potentially useful in biofortification strategies

Iron biofortification in rice: an update on quantitative trait loci and candidate genes

ReviewRice is the most versatile model for cereals and also an economically relevant food crop; as a result, it is the most suitable species for molecular characterization of Fe homeostasis and biofortification. Recently there have been significant efforts to dissect genes and quantitative trait loci (QTL) associated with Fe translocation into rice grains; such information is highly useful for Fe biofortification of cereals but very limited in other species, such as maize (Zea mays) and wheat (Triticum aestivum). Given rice’s centrality as a model for Poaceae species, we review the current knowledge on genes playing important roles in Fe transport, accumulation, and distribution in rice grains and QTLs that might explain the variability in Fe concentrations observed in different genotypes. More than 90 Fe QTLs have been identified over the 12 rice chromosomes. From these, 17 were recorded as stable, and 25 harbored Fe-related genes nearby or within the QTL. Among the candidate genes associated with Fe uptake, translocation, and loading into rice grains, we highlight the function of transporters from the YSL and ZIP families; transporters from metal-binding molecules, such as nicotianamine and deoxymugineic acid; vacuolar iron transporters; citrate efflux transporters; and others that were shown to play a role in steps leading to Fe delivery to seeds. Finally, we discuss the application of these QTLs and genes in genomics assisted breeding for fast-tracking Fe biofortification in rice and other cereals in the near futureinfo:eu-repo/semantics/publishedVersio

Ecological Systems in the Contexts of Foster Care and Parental Incarceration

This bundled dissertation sought to advance research about children in the contexts of foster care and/or parental incarceration. The first study, “Developmental Differences in Children\u27s Visits with Their Parents in Jail,†expanded upon previous research of young children\u27s experiences visiting jail by including a sample of 3-17-year-olds. The study described children\u27s visitation through quantitative (measured by the Jail-Prison Observation Checklist) and qualitative (themes gleaned from observations of children\u27s visits with parents in jail) analyses with an ecological systems framework. The second longitudinal study, “Visits with Fathers Involved in the Criminal Justice System and Behavioral Outcomes among Children in Foster Care,†considered individual (i.e., race, gender, emotional and behavioral adjustment) and microsystem factors (i.e., visitation with parents who are incarcerated) to inform recommendations for correctional systems and policymakers in the exosystems, macrosystems, and chronosystems. Finally, few studies consider greater ecological systems and the well-being of children in foster care. As such, the third study, titled Ecological Disruptions and Well-being among Children in Foster Care, examined the impact of multiple ecological disruptions (i.e., changes in or separation from siblings, friends, school, church, community) on children\u27s internalizing symptoms and externalizing behaviors over time. The combination of these three studies increases understanding about the complex, interactive factors that impact the well-being of children in the contexts of child welfare and/or parental incarceration

The Effects of a Montessori-Based Activity on Affect and Engagement in Persons with Dementia

Activities for persons with dementia can provide many benefits such as cognitive and social stimulation and reduced agitation. Montessori-based activities are designed with specific principles in mind such as building on existing skills, repetition, and making tasks that are self-correcting. The purpose of this study was to compare the effects of a Montessori-based group activity to typically-offered activities in terms of the amount of active engagement and positive affect displayed. Eight individuals diagnosed with dementia and who lived in a memory care unit participated in the study. The study used an A-B-A- B experimental design where baseline (A) involved observing activities typically offered in the facility and the intervention (B) involved participants playing a Montessori-based activity called “Memory Bingo”. Memory Bingo consists of four cards containing a picture and word linking to corresponding cards that are called out. If participants have the matching card, they flip it over. The game continues until someone flips over all their cards. During both phases, participant’s affect and engagement were recorded through direct observation. Data collection is 90% complete. Thus far, the Montessori-based activity appears to yield greater levels of active engagement compared to typically-offered activities.Positive affect, however, has remained relatively steady throughout all phases. There is a need for new activity programs that are interesting and engaging to individuals with dementia. Montessori-based activities, such as Memory Bingo, may offer a promising alternative approach to activity programming that may result in increased enjoyment and participation in activities for persons with dementia