Loyola eCommons

    Lactobacillus crispatus Produces a Bacteridical Molecule That Kills Uropathogenic E. Coli

    Get PDF
    As many as 1 in 2 women will have at least one urinary tract infection (UTI) in their lifetime. UTIs can cause complications in pregnancy and decrease quality of life, and their treatment and prevention are expensive. Uropathogenic E. coli (UPEC) is the primary cause of UTI. The probiotic and bactericidal capacities of gut and vaginal Lactobacillus isolates have been studied, but the same attention has not been paid to urinary strains. These urinary isolates of L. crispatus appear to have a greater killing capacity against UPEC and this bactericidal activity does not depend on the cells themselves, consistent with the hypothesis that they secrete a molecule with anti-UPEC activity. In the future, this bacterium could be useful as a probiotic and molecules it produces could be used as antibacterial compounds. The SCS of one urinary isolate of L. crispatus killed several logs of UPEC within 2 hours of exposure. This isolate creates a more acidic environment than isolates of other Lactobacillus species, but the killing of UPEC was not due to low pH alone, as buffered of the SCS delayed but did not eliminate the bactericidal effect. This effect became stronger after the SCS was left to sit for 24 hours. The molecule was not heat sensitive. A urinary L. crispatus isolate produces a unique soluble molecule that can kill up to 9 logs of UPEC within 24 hours. The molecule may be an antimicrobial peptide or bacteriocin. Further experiments are required

    Privatizing Laws: Examining the Relationship between Privatization Elements in State Education Laws and Fourth Grade Academic Performance

    Get PDF
    In the midst of a global context tied to expanding market connections, the United States adopted privatized education reform that has only been increasing over the years. Specific states have adopted privatization in varying degrees and forms, including vouchers, charter schools, public-private partnerships, contracting out services, and virtual education. States have implemented privatization forms for different reasons, such as to improve student achievement, save money, or provide for increased student choice. This research study evaluates the impact of privatization reforms on student achievement by examining the relationship between various privatization elements in state laws and student achievement. To do so, this study employed mixed methods to code and obtain privatization data and assess the states’ student achievement levels in math and reading as demonstrated on the NAEP in 2015. Aligning with the generally mixed findings of privatization reform efforts on student achievement, this study produced mixed results. The results mainly showed negative relationships between privatization elements and student achievement, but the results were statistically insignificant. Thus, based on the mixed and insignificant relationship results, privatization may not be an effective solution to improve student achievement on its own. Keywords: Privatization; Reform; Education; Education Policy; School Choice; Charter Schools; School Vouchers; Contracts; Public-Private Partnerships; Virtual Schools; Online Classes; State Laws; Student Achievement Keywords: Privatization; Reform; Education; Education Policy; School Choice; Charter Schools; School Vouchers; Contracts; Public-Private Partnership; Virtual Schools; Online Classes; State Laws; Student Achievemen

    Role of the Interleukin-22 and STAT3 Signaling Pathway in Gut Barrier Maintenance Following Intoxication and Burn Injury

    Get PDF
    More than half of patients admitted to burn centers for treatment have measurable alcohol levels in their blood. Intoxication that precedes a traumatic burn injury leads to worsened patient outcomes, including higher rates of infection and sepsis. The intestines harbor the largest concentration of bacteria in the human host. Any disruption of the intestinal barrier as a result of intoxication and burn injury could contribute to infection and/or sepsis from gut-derived microbes. Previous work has demonstrated that the intestinal barrier becomes leaky, and that administration of interleukin-22 (IL-22) significantly reduces gut barrier leakiness following alcohol and burn injury. However, the cellular and molecular mechanism by which intestinal barrier leakiness occurs, and how IL-22 mediates intestinal barrier protection remain largely unknown. The overall objectives of this dissertation are to identify the mechanisms of intestine epithelial barrier disruption, and to elucidate how IL-22 protects the intestine epithelial barrier following alcohol and burn injury. The hypothesis of this project is IL-22 utilizes STAT3 signaling in intestine epithelial cells to promote barrier regeneration and restore the intestinal microbiome following alcohol and burn injury. Our results demonstrate disruptions to the intestine occur on many levels, including diminished claudin-8 and F-actin tight junction formation between epithelial cells, reduced numbers of proliferating and higher incidence of epithelial cell death, and large overgrowth of bacteria within the lumen of the small intestine. Interleukin-22 is able to restore numbers of proliferating cells to promote intestine barrier regeneration, and significantly elevates antimicrobial peptide (AMP) gene expression while mitigating bacterial overgrowth. Transgenic mice lacking the signal transducer and activator of transcription factor 3 (STAT3) in intestine epithelial cells lose the protective effects of IL-22 administration. Together, our data demonstrate that IL-22 signals through STAT3 to protect the intestine barrier by promoting intestinal epithelial cell proliferation, enhancing AMP gene expression, and preventing bacterial overgrowth following alcohol and burn injury

    Cura Personalis e Pedagogia Inaciana

    No full text

    Reflexion, pedagogia y educacion

    No full text

    Curso Especial de Pedagogia Ignaciana

    No full text
    Loyola eCommons is based in US
    Do you manage Loyola eCommons? Access insider analytics, issue reports and manage access to outputs from your repository in the CORE Dashboard!