Peroxisomal alanine : glyoxylate aminotransferase (AGT1) is a photorespiratory enzyme with multiple substrates in Arabidopsis thaliana

At least two glyoxylate aminotransferases are hypothesized to participate in the steps of photorespiration located in peroxisomes. Until recently, however, genes encoding these enzymes had not been identified. We describe the isolation and characterization of an alanine : glyoxylate aminotransferase ( AGT1 , formerly AGT ) cDNA from Arabidopsis thaliana . Southern blot analysis confirmed that Arabidopsis AGT1 is encoded by a single gene. Homologs of this class IV aminotransferase are also known in other plants, animals, and methylotrophic bacteria, suggesting an ancient evolutionary origin of this enzyme. AGT1 transcripts were present in all tissues of Arabidopsis , but were most abundant in green, leafy tissues. Purified, recombinant Arabidopsis AGT1 expressed in Escherichia coli catalyzed three transamination reactions using the following amino donor : acceptor combinations: alanine : glyoxylate, serine : glyoxylate, and serine : pyruvate. AGT1 had the highest specific activity with the serine : glyoxylate transamination, and apparent K m measurements indicate that this is the preferred in vivo reaction. In vitro import experiments and subcellular fractionations localized AGT1 to peroxisomes. Sequence analysis of the photorespiratory sat mutants revealed a single nucleotide substitution in the AGT1 gene from these plants. This transition mutation is predicted to result in a proline-to-leucine substitution at residue 251 of AGT1. When this mutation was engineered into the recombinant AGT1 protein, enzymatic activity using all three donor : acceptor pairs was abolished. We conclude that Arabidopsis AGT1 is a peroxisomal photorespiratory enzyme that catalyzes transamination reactions with multiple substrates.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73264/1/j.1365-313x.2001.00961.x.pd

Linear/circular spectropolarimetry of diffuse interstellar bands

Context. The identification of the carriers of diffuse interstellar bands (DIBs) remains one of the long-standing mysteries in astronomy. The detection of a polarisation signal in a DIB profile can be used to distinguish between a dust or gas-phase carrier. The polarisation profile can give additional information on the grain or molecular properties of the absorber. In order to detect and measure the linear and circular polarisation of the DIBs we observed reddened lines of sight showing continuum polarisation. For this study we selected two stars HD 197770 and HD 194279. We used high-resolution (R~64.000) spectropolarimetry in the wavelength range from 3700 to 10480 Angstrom with the ESPaDOnS echelle spectrograph mounted at the CFHT. Results. High S/N and high resolution Stokes V (circular), Q and U (linear) spectra were obtained. We constrained upper limits by a factor of 10 for previously observed DIBs. Furthermore, we analysed ~30 additional DIBs for which no spectropolarimetry data has been obtained before. This included the 9577 A DIB and the 8621 A DIB. Conclusions. The lack of polarisation in 45 DIB profiles suggests that none of the absorption lines is induced by a grain-type carrier. The strict upper limits, less than ~0.01%, derived for the observed lines-of-sight imply that if DIBs are due to gas-phase molecules these carriers have polarisation efficiencies which are at least 6 times, and up to 300 times, smaller than those predicted for grain-related carriers.Comment: 6 pages + 13 pages online material, submitted to A&

Nanostructure of cellulose microfibrils in spruce wood

The structure of cellulose microfibrils in wood is not known in detail, despite the abundance of cellulose in woody biomass and its importance for biology, energy, and engineering. The structure of the microfibrils of spruce wood cellulose was investigated using a range of spectroscopic methods coupled to small-angle neutron and wide-angle X-ray scattering. The scattering data were consistent with 24-chain microfibrils and favored a “rectangular” model with both hydrophobic and hydrophilic surfaces exposed. Disorder in chain packing and hydrogen bonding was shown to increase outwards from the microfibril center. The extent of disorder blurred the distinction between the I alpha and I beta allomorphs. Chains at the surface were distinct in conformation, with high levels of conformational disorder at C-6, less intramolecular hydrogen bonding and more outward-directed hydrogen bonding. Axial disorder could be explained in terms of twisting of the microfibrils, with implications for their biosynthesis

Number Counts of Bright Extremely Red Objects: Evolved Massive Galaxies at z~1

We present results on number counts of Extremely Red Objects (EROs) in a 2850 arcmin^2 near-infrared survey performed in European Large Area ISO Survey (ELAIS) fields at K<17.5. Counts of EROs are extended to brighter levels than available previously, giving 0.002 +/- 0.001 arcmin^-2 at K<16.5 and consistent numbers with literature values at fainter magnitudes. Photometric redshifts from HYPERZ as well as GRASIL model SEDs of galaxies imply that our EROs are located in the range z=0.7-1.5, with the bulk of the population at z~1. Taking advantage of the ISO data in the fields, we use mid-IR detections to constrain the number of dusty EROs, and also discuss the superior capabilities of Spitzer Space Telescope to detect dusty EROs. Both the mid-IR data and the use of colour-colour diagrammes indicate that at most 10-20% of the EROs in this bright regime are dusty starbursting systems. The space density of our EROs, interpreted to be counterparts of local >2-3L^star massive galaxies at around z~1, is estimated to be approx. 2x10^-5 Mpc^-3, which is consistent with local values. Furthermore, the cumulative number counts at our bright magnitudes are remarkably well fitted by pure luminosity evolution models.Comment: A&A, accepted, 14 pages and 8 ps-fig

Ionizing Photon Escape Fractions from High Redshift Dwarf Galaxies

It has been argued that low-luminosity dwarf galaxies are the dominant source of ionizing radiation during cosmological reionization. The fraction of ionizing radiation that escapes into the intergalactic medium from dwarf galaxies with masses less than ~10^9.5 solar masses plays a critical role during this epoch. Using an extensive suite of very high resolution (0.1 pc), adaptive mesh refinement, radiation hydrodynamical simulations of idealized and cosmological dwarf galaxies, we characterize the behavior of the escape fraction in galaxies between 3 x 10^6 and 3 x 10^9 solar masses with different spin parameters, amounts of turbulence, and baryon mass fractions. For a given halo mass, escape fractions can vary up to a factor of two, depending on the initial setup of the idealized halo. In a cosmological setting, we find that the time-averaged photon escape fraction always exceeds 25% and reaches up to 80% in halos with masses above 10^8 solar masses with a top-heavy IMF. The instantaneous escape fraction can vary up to an order of magnitude in a few million years and tend to be positively correlated with star formation rate. We find that the mean of the star formation efficiency times ionizing photon escape fraction, averaged over all atomic cooling (T_vir > 8000 K) galaxies, ranges from 0.02 for a normal IMF to 0.03 for a top-heavy IMF, whereas smaller, molecular cooling galaxies in minihalos do not make a significant contribution to reionizing the universe due to a much lower star formation efficiency. These results provide the physical basis for cosmological reionization by stellar sources, predominately atomic cooling dwarf galaxies.Comment: 17 pages, 12 figures, accepted to ApJ, minor change

Gene expression and matrix turnover in overused and damaged tendons

Chronic, painful conditions affecting tendons, frequently known as tendinopathy, are very common types of sporting injury. The tendon extracellular matrix is substantially altered in tendinopathy, and these changes are thought to precede and underlie the clinical condition. The tendon cell response to repeated minor injuries or “overuse” is thought to be a major factor in the development of tendinopathy. Changes in matrix turnover may also be effected by the cellular response to physical load, altering the balance of matrix turnover and changing the structure and composition of the tendon. Matrix turnover is relatively high in tendons exposed to high mechanical demands, such as the supraspinatus and Achilles, and this is thought to represent either a repair or tissue maintenance function. Metalloproteinases are a large family of enzymes capable of degrading all of the tendon matrix components, and these are thought to play a major role in the degradation of matrix during development, adaptation and repair. It is proposed that some metalloproteinase enzymes are required for the health of the tendon, and others may be damaging, leading to degeneration of the tissue. Further research is required to investigate how these enzyme activities are regulated in tendon and altered in tendinopathy. A profile of all the metalloproteinases expressed and active in healthy and degenerate tendon is required and may lead to the development of new drug therapies for these common and debilitating sports injuries

Merger rates of dark matter haloes: a comparison between EPS and N-body results

We calculate merger rates of dark matter haloes using the Extended Press-Schechter approximation (EPS) for the Spherical Collapse (SC) and the Ellipsoidal Collapse (EC) models. Merger rates have been calculated for masses in the range $10^{10}M_{\odot}\mathrm{h}^{-1}$ to $10^{14}M_{\odot}\mathrm{h}^{-1}$ and for redshifts $z$ in the range 0 to 3 and they have been compared with merger rates that have been proposed by other authors as fits to the results of N-body simulations. The detailed comparison presented here shows that the agreement between the analytical models and N-body simulations depends crucially on the mass of the descendant halo. For some range of masses and redshifts either SC or EC models approximate satisfactory the results of N-body simulations but for other cases both models are less satisfactory or even bad approximations. We showed, by studying the parameters of the problem that a disagreement --if it appears-- does not depend on the values of the parameters but on the kind of the particular solution used for the distribution of progenitors or on the nature of EPS methods. Further studies could help to improve our understanding about the physical processes during the formation of dark matter haloes.Comment: 29 pages, 9 figure

Herschel-ATLAS: Multi-wavelength SEDs and physical properties of 250 micron-selected galaxies at z < 0.5

We present a pan-chromatic analysis of an unprecedented sample of 1402 250 micron-selected galaxies at z < 0.5 (mean z = 0.24) from the Herschel-ATLAS survey. We complement our Herschel 100-500 micron data with UV-K-band photometry from the Galaxy And Mass Assembly (GAMA) survey and apply the MAGPHYS energy-balance technique to produce pan-chromatic SEDs for a representative sample of 250 micron selected galaxies spanning the most recent 5 Gyr of cosmic history. We derive estimates of physical parameters, including star formation rates, stellar masses, dust masses and infrared luminosities. The typical H-ATLAS galaxy at z < 0.5 has a far-infrared luminosity in the range 10^10 - 10^12 Lsolar (SFR: 1-50 Msolar/yr) thus is broadly representative of normal star forming galaxies over this redshift range. We show that 250 micron-selected galaxies contain a larger mass of dust at a given infra-red luminosity or star formation rate than previous samples selected at 60 micron from IRAS. We derive typical SEDs for H-ATLAS galaxies, and show that the emergent SED shape is most sensitive to specific star formation rate. The optical-UV SEDs also become more reddened due to dust at higher redshifts. Our template SEDs are significantly cooler than existing infra-red templates. They may therefore be most appropriate for inferring total IR luminosities from moderate redshift submillimetre selected samples and for inclusion in models of the lower redshift submillimetre galaxy populations.Comment: 26 pages, 24 figures, Accepted by MNRA

Neural correlates of sexual cue reactivity in individuals with and without compulsive sexual behaviours

Although compulsive sexual behaviour (CSB) has been conceptualized as a "behavioural" addiction and common or overlapping neural circuits may govern the processing of natural and drug rewards, little is known regarding the responses to sexually explicit materials in individuals with and without CSB. Here, the processing of cues of varying sexual content was assessed in individuals with and without CSB, focusing on neural regions identified in prior studies of drug-cue reactivity. 19 CSB subjects and 19 healthy volunteers were assessed using functional MRI comparing sexually explicit videos with non-sexual exciting videos. Ratings of sexual desire and liking were obtained. Relative to healthy volunteers, CSB subjects had greater desire but similar liking scores in response to the sexually explicit videos. Exposure to sexually explicit cues in CSB compared to non-CSB subjects was associated with activation of the dorsal anterior cingulate, ventral striatum and amygdala. Functional connectivity of the dorsal anterior cingulate-ventral striatum-amygdala network was associated with subjective sexual desire (but not liking) to a greater degree in CSB relative to non-CSB subjects. The dissociation between desire or wanting and liking is consistent with theories of incentive motivation underlying CSB as in drug addictions. Neural differences in the processing of sexual-cue reactivity were identified in CSB subjects in regions previously implicated in drug-cue reactivity studies. The greater engagement of corticostriatal limbic circuitry in CSB following exposure to sexual cues suggests neural mechanisms underlying CSB and potential biological targets for interventions