Development and evaluation of plastic based solar still for production of distilled water

Solar still was developed and evaluated at Department of Renewable Energy Engineering, College of Agricultural Engineering and Technology, Dediapada. The average yield of distilled water in developed solar still varied from 1055-1498 ml/m 2 -day during winter and summer where as in already developed still varied 1350 to 1550 ml /m 2 day . Thermal efficiency of developed solar still was found as 20 per cent .The physicochemical analysis was carried out to examine the effect of distillation on tap water. A drastic reduction in the TDS, Chlorides, Calcium hardness and magnesium hardness, dissolved silica was observed through solar distillation. The payback period of the unit was only 6 months and after that period the unit produced net profit. The manufacturing cost of this developed solar still was only Rs. 1640/- which was totally manufactured in plastic material so no any corrosive material came in contact for changing the property of distilled water as output. Cleaning of solar still could be easily carried out by just removing the w shape dome of developed device which cannot be possible in available solar still in market

Bivariate tt-distribution for transition matrix elements in Breit-Wigner to Gaussian domains of interacting particle systems

Interacting many-particle systems with a mean-field one body part plus a chaos generating random two-body interaction having strength $\lambda$, exhibit Poisson to GOE and Breit-Wigner (BW) to Gaussian transitions in level fluctuations and strength functions with transition points marked by $\lambda=\lambda_c$ and $\lambda=\lambda_F$, respectively; $\lambda_F >> \lambda_c$. For these systems theory for matrix elements of one-body transition operators is available, as valid in the Gaussian domain, with $\lambda > \lambda_F$, in terms of orbitals occupation numbers, level densities and an integral involving a bivariate Gaussian in the initial and final energies. Here we show that, using bivariate $t$-distribution, the theory extends below from the Gaussian regime to the BW regime up to $\lambda=\lambda_c$. This is well tested in numerical calculations for six spinless fermions in twelve single particle states.Comment: 7 pages, 2 figure

The possible significance of trisomy 8 in acute myeloid leukemia

Background: Acute myeloid leukemia (AML) is a heterogeneous disorder that results from a block in the differentiation of haematopoietic progenitor cells along with uncontrolled proliferation. Trisomy 8 is the most common recurring numerical chromosomal aberrations in acute myeloid leukemia (AML). It occurs either as a sole anomaly or together with other additional chromosomal aberrations. The prognostic significance of trisomy 8 in presence of other additional chromosomal abnormality depends on clonal cytogenetic changes. The patients with trisomy 8 had shorter survival with significantly increased risk with other chromosomal abnormality.Methods: Total 139 patients were screened between January 2016 to November 2016 who were suspected of AML cases. Bone marrow cultures were set up using conventional cytogenetic methods. Chromosomal preparation was made and subjected to GTG banding technique. Banded metaphases were analysed and karyotyped for further analysis.Results: Cytogenetic evaluation of karyotyped of 139 suspected AML patients showed 52 with t(8;21)(q22;q22), 36 with t(15;17)(q22;q12), and 11 with inv(16)(p13;q22). The rest 40 cases found with additional chromosomal abnormalities, of which 16 were sole trisomy 8 and 24 cases were found with other chromosomal abnormalities In addition, only one person found with t(8;21) and trisomy 8, while  three person having t(15;17) with trisomy 8.Conclusions: AML is considered to be one of the most important cytogenetic prognostic determinants. Recurrent chromosomal translocation with trisomy 8 varying 1.9% for t(8;21) and 8.3% for t(15;17). In the present study trisomy 8 in AML with known favourable anomalies is very small. Therefore, it cannot be taken as a prognostic marker

Random matrix ensemble with random two-body interactions in presence of a mean-field for spin one boson systems

For $m$ number of bosons, carrying spin ($S$=1) degree of freedom, in $\Omega$ number of single particle orbitals, each triply degenerate, we introduce and analyze embedded Gaussian orthogonal ensemble of random matrices generated by random two-body interactions that are spin (S) scalar [BEGOE(2)-$S1$]. The embedding algebra is $U(3) \supset G \supset G1 \otimes SO(3)$ with SO(3) generating spin $S$. A method for constructing the ensembles in fixed-($m$, $S$) space has been developed. Numerical calculations show that the form of the fixed-($m$, $S$) density of states is close to Gaussian and level fluctuations follow GOE. Propagation formulas for the fixed-($m$, $S$) space energy centroids and spectral variances are derived for a general one plus two-body Hamiltonian preserving spin. In addition to these, we also introduce two different pairing symmetry algebras in the space defined by BEGOE(2)-$S1$ and the structure of ground states is studied for each paring symmetry.Comment: 22 pages, 6 figure

Wigner--Dyson statistics for a class of integrable models

We construct an ensemble of second--quantized Hamiltonians with two bosonic degrees of freedom, whose members display with probability one GOE or GUE statistics. Nevertheless, these Hamiltonians have a second integral of motion, namely the boson number, and thus are integrable. To construct this ensemble we use some ``reverse engineering'' starting from the fact that $n$--bosons in a two--level system with random interactions have an integrable classical limit by the old Heisenberg association of boson operators to actions and angles. By choosing an $n$--body random interaction and degenerate levels we end up with GOE or GUE Hamiltonians. Ergodicity of these ensembles completes the example.Comment: 3 pages, 1 figur

Influence of peripartum dietary supplementation of choline and fat in protected form on production performance of Gir cows

In this experiment, the effects of supplementing choline and fat in rumen protected form during peripartum period on feed intake, milk production and composition of Gir cows were studied. Twenty four Gir cows of 1st to 3rd parity were used from 30 days pre-partum through 60 days post-partum and randomly assigned to four equal treatment groups (n=6) on the basis of their parity, body weight and previous lactation yield. Control diet was fed to cows in group T1 (control). Additionally, rumen protected choline (RPC) @45 g/d in group T2; rumen protected fat (RPF) 80 g/d in group T3 and RPC @45 g/d + RPF @80 g/d in group T4 were supplemented along with control diet. The treatments significantly affected dry mater intake (DMI) and milk production of cows. DMI was increased in the cows fed with RPC as compared to control. Milk yield, 4% fat-corrected milk, solid-corrected milk and energy- corrected milk were higher in the cows fed with RPC and RPF alone or in combination, as compared to control. No synergistic effect was observed with these supplements on DMI or milk production. None of these supplements influenced the milk composition significantly, however yield of milk fat, protein and lactose were higher in all the nutrient supplemented cows compared to control. Net return over feed cost was higher in supplemented cows compared to control. Results indicated that supplementation of RPC or RPF can improve feed intake and productive performance of Gir cows for overall economic benefits

Increased Immune-Regulatory Receptor Expression on Effector T Cells as Early Indicators of Relapse Following Autologous Stem Cell Transplantation for Multiple Myeloma

The benefit of autologous stem cell transplantation (ASCT) in newly diagnosed myeloma patients, apart from supporting high dose chemotherapy, may include effects on T cell function in the bone marrow (BM). We report our exploratory findings on marrow infiltrating T cells early post-ASCT (day+100), examining phenotype and T cell receptor (TCR) repertoire, seeking correlations with timing of relapse. Compared to healthy donors (HD), we observed an increase in regulatory T cells (CD4+FoxP3+, Tregs) with reduction in CD4 T cells, leading to lower CD4:8 ratios. Compared to paired pre-treatment marrow, both CD4 and CD8 compartments showed a reduction in naïve, and increase in effector memory subsets, suggestive of a more differentiated phenotype. This was supported by increased levels of several immune-regulatory and activation proteins (ICOS, PD-1, LAG-3, CTLA-4 and GzmB) when compared with HD. Unsupervised analysis identified a patient subgroup with shorter PFS (p=0.031) whose BM contained increased Tregs, and higher immune-regulatory markers (ICOS, PD-1, LAG-3) on effector T cells. Using single feature analysis, higher frequencies of marrow PD-1+ on CD4+FoxP3- cells and Ki67+ on CD8 cells were independently associated with early relapse. Finally, studying paired pre-treatment and post-ASCT BM (n=5), we note reduced abundance of TCR sequences at day+100, with a greater proportion of expanded sequences indicating a more focused persistent TCR repertoire. Our findings indicate that, following induction chemotherapy and ASCT, marrow T cells demonstrate increased activation and differentiation, with TCR repertoire focusing. Pending confirmation in larger series, higher levels of immune-regulatory proteins on T cell effectors at day+100 may indicate early relapse

Expression of Regulatory Platelet MicroRNAs in Patients with Sickle Cell Disease

Background: Increased platelet activation in sickle cell disease (SCD) contributes to a state of hypercoagulability and confers a risk of thromboembolic complications. The role for post-transcriptional regulation of the platelet transcriptome by microRNAs (miRNAs) in SCD has not been previously explored. This is the first study to determine whether platelets from SCD exhibit an altered miRNA expression profile. Methods and Findings: We analyzed the expression of miRNAs isolated from platelets from a primary cohort (SCD = 19, controls = 10) and a validation cohort (SCD = 7, controls = 7) by hybridizing to the Agilent miRNA microarrays. A dramatic difference in miRNA expression profiles between patients and controls was noted in both cohorts separately. A total of 40 differentially expressed platelet miRNAs were identified as common in both cohorts (p-value 0.05, fold change>2) with 24 miRNAs downregulated. Interestingly, 14 of the 24 downregulated miRNAs were members of three families - miR-329, miR-376 and miR-154 - which localized to the epigenetically regulated, maternally imprinted chromosome 14q32 region. We validated the downregulated miRNAs, miR-376a and miR-409-3p, and an upregulated miR-1225-3p using qRT-PCR. Over-expression of the miR-1225-3p in the Meg01 cells was followed by mRNA expression profiling to identify mRNA targets. This resulted in significant transcriptional repression of 1605 transcripts. A combinatorial approach using Meg01 mRNA expression profiles following miR-1225-3p overexpression, a computational prediction analysis of miRNA target sequences and a previously published set of differentially expressed platelet transcripts from SCD patients, identified three novel platelet mRNA targets: PBXIP1, PLAGL2 and PHF20L1. Conclusions: We have identified significant differences in functionally active platelet miRNAs in patients with SCD as compared to controls. These data provide an important inventory of differentially expressed miRNAs in SCD patients and an experimental framework for future studies of miRNAs as regulators of biological pathways in platelets. © 2013 Jain et al