Dodatak supranutritivnih doza cinkova sulfata i bakterija Bacillus firmus ubijenih toplinom u obrok rano odbijenih svinja: utjecaj na rast, funkciju neutrofila i upalne citokine

The objective of the study was to investigate the effect of supplementation of a supranutritional dose of zinc sulfate (ZnSO4) and Bacillus firmus derived bio-response modifier (BRM) on growth, blood neutrophil functions, pro-inflammatory and anti-inflammatory cytokine responses in early weanling piglets. In total, 45 piglets (age of 19.25 ± 0.84 days) were randomly divided into five groups: I (basal diet only), II (basal diet supplemented with ZnSO4), III (basal diet supplemented with BRM), IV (basal diet supplemented with ZnSO4 plus BRM) and V (basal diet without weaning from dam). The production of myeloperoxidase (MPO) and superoxide anion (O2-), and the concentration of transforming growth factor-β1 (TGF-β1) were markedly reduced, whereas the concentrations of intercellular adhesion molecule 1 (ICAM1), and monocyte chemoattractant protein 1 (MCP1) were significantly (P0.05) but a marked increase (P0,05), ali kod prasadi iz skupine II. i III. je 14. i 7. dana opaženo znakovito povišenje (P<0,05) ICAM1, MCP1, te sniženje koncentracije TGF-β1. U prasadi iz skupine IV dodavanje BRM i ZnSO4 u osnovnom obroku poboljšalo je MPO (2. dan) i O2- (7. dan), bez znakovitih promjena u rastu i koncentraciji citokina. Na kraju, zaključeno je da dodavanje kombinacije BRM i ZnSO4 u obroku potiče urođenu imunost prasadi što nije slučaj kada se ZnSO4 ili BRM dodaju zasebno. Rezultati ovog istraživanja pomoći će u formuliranju učinkovitog upravljanja hranidbom kod rano odbijene prasadi u krdu svinja

Genetic manipulation of endosymbionts to control vector and vector borne diseases

Vector borne diseases (VBD) are on the rise because of failure of the existing methods of control of vector and vector borne diseases and the climate change. A steep rise of VBDs are due to several factors like selection of insecticide resistant vector population, drug resistant parasite population and lack of effective vaccines against the VBDs. Environmental pollution, public health hazard and insecticide resistant vector population indicate that the insecticides are no longer a sustainable control method of vector and vector-borne diseases. Amongst the various alternative control strategies, symbiont based approach utilizing endosymbionts of arthropod vectors could be explored to control the vector and vector borne diseases. The endosymbiont population of arthropod vectors could be exploited in different ways viz., as a chemotherapeutic target, vaccine target for the control of vectors. Expression of molecules with antiparasitic activity by genetically transformed symbiotic bacteria of disease-transmitting arthropods may serve as a powerful approach to control certain arthropod-borne diseases. Genetic transformation of symbiotic bacteria of the arthropod vector to alter the vector&amp;#8217;s ability to transmit pathogen is an alternative means of blocking the transmission of VBDs. In Indian scenario, where dengue, chikungunya, malaria and filariosis are prevalent, paratransgenic based approach can be used effectively.&lt;i&gt;&lt;/i&gt; [Vet World 2012; 5(9.000): 571-576

Metabolism of aceclofenac to diclofenac in the domestic water buffalo Bubalus bubalis confirms it as a threat to Critically Endangered Gyps vultures in South Asia

Vulture declines in South Asia were caused by accidental poisoning by the veterinary non-steroidal anti-inflammatory drug (NSAID) diclofenac. Although veterinary use of diclofenac has been banned, other vulture-toxic NSAIDs are legally available, including aceclofenac, which has been shown to metabolise into diclofenac in domestic cattle. We gave nine domestic water buffalo the recommended dose of aceclofenac (2 mg kg−1 body weight), collected blood at intervals up to 48 h, and carried out a pharmacokinetic analysis of aceclofenac and its metabolite diclofenac in plasma. Aceclofenac was rapidly converted to diclofenac, and was barely detectable in plasma at any sampling time. Diclofenac was present within 20 min, and peaked 4–8 h after dosing. Aceclofenac is a prodrug of diclofenac, and behaves similarly in domestic water buffalo as it did in domestic cattle, posing the same risk to vultures. We recommend an immediate ban on the veterinary use of aceclofenac across vulture-range countries.The Haryana Forest Development Corporation.https://www.elsevier.com/locate/etap2023-09-30hj2023Paraclinical Science

Metabolism of aceclofenac to diclofenac in the domestic water buffalo Bubalus bubalis confirms it as a threat to Critically Endangered Gyps vultures in South Asia.

Vulture declines in South Asia were caused by accidental poisoning by the veterinary non-steroidal anti-inflammatory drug (NSAID) diclofenac. Although veterinary use of diclofenac has been banned, other vulture-toxic NSAIDs are legally available, including aceclofenac, which has been shown to metabolise into diclofenac in domestic cattle. We gave nine domestic water buffalo the recommended dose of aceclofenac (2 mg kg-1 body weight), collected blood at intervals up to 48 h, and carried out a pharmacokinetic analysis of aceclofenac and its metabolite diclofenac in plasma. Aceclofenac was rapidly converted to diclofenac, and was barely detectable in plasma at any sampling time. Diclofenac was present within 20 min, and peaked 4-8 h after dosing. Aceclofenac is a prodrug of diclofenac, and behaves similarly in domestic water buffalo as it did in domestic cattle, posing the same risk to vultures. We recommend an immediate ban on the veterinary use of aceclofenac across vulture-range countries.Funding was provided by the Haryana Forest Development Corporation