224 research outputs found
Direct mapping rather than motor prediction subserves modulation of corticospinal excitability during observation of actions in real time
Motor facilitation refersto the specific increment in corticospinal excitability (CSE)elicited by the observation of actions performed by others. To date,the precise nature of the mechanism at the basis of this phenomenonis unknown. One possibility is that motor facilitation is driven by apredictive process reminiscent of the role of forward models in motorcontrol. Alternatively, motor facilitation may result from a model-freemechanism by which the basic elements of the observed action aredirectly mapped onto their cortical representations. Our study wasdesigned to discern these alternatives. To this aim, we recorded thetime course of CSE for the first dorsal interosseous (FDI) and theabductor digiti minimi (ADM) during observation of three graspingactions in real time, two of which strongly diverged in kinematicsfrom their natural (invariant) form. Although artificially slow movementsused in most action observation studies might enhance theobserver?s discrimination performance, the use of videos in real timeis crucial to maintain the time course of CSE within the physiologicalrange of daily actions. CSE was measured at 4 time points within a240-ms window that best captured the kinematic divergence from theinvariant form. Our results show that CSE of the FDI, not the ADM,closely follows the functional role of the muscle despite the mismatchbetween the natural and the divergent kinematics. We propose thatmotor facilitation during observation of actions performed in real timereflects the model-free coding of perceived movement following adirect mapping mechanism.Fil: Gueugneau, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Mc Cabe, Sofia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Villalta, Jorge Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Grafton, Scott T.. University of California. Department of Psychology; Estados UnidosFil: Della Maggiore, Valeria Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentin
Modelling pyroclastic density currents of the April 2021 La Soufrière St. Vincent eruption: from rapid invasion maps to field-constrained numerical simulations
Abstract
The April 2021 La Soufrière of St. Vincent eruption generated several pyroclastic density currents (PDCs) during the two weeks of the crisis, from April 9
th
to April 22
nd
. To support the hazard assessment team during this eruption, numerical simulations were performed in real time and generated rapid scenario-based PDC invasion maps with the two-phase version of the code VolcFlow, which was able to simulate both the concentrated and dilute regime of PDCs. To generate the maps, only the source properties (shape and location) and the initial volume used to generate the PDCs were varied, all other input parameters were kept constant and estimated from previous simulations. New simulations were then performed based on the field-based deposit map to assess the code's ability to simulate such PDCs. Results show that the syn-crisis invasion maps satisfactorily mimic the observed valley-confined PDCs, while unconfined dilute PDCs were overestimated. The result also highlight that simulation results are greatly improved with additional field-based data, which help constrain the PDC sequence. Numerous lessons were learned, including: (i) how to choose the most critical input parameters, (ii) the importance of syn-eruptive radar imagery, and (iii) the potential of this two-phase model for rapid hazard assessment purposes.
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The Role of Muscle Perfusion in the Age-Associated Decline of Mitochondrial Function in Healthy Individuals
Maximum oxidative capacity of skeletal muscle measured by in vivo phosphorus magnetic resonance spectroscopy (31P-MRS) declines with age, and negatively affects whole-body aerobic capacity. However, it remains unclear whether the loss of oxidative capacity is caused by reduced volume and function of mitochondria or limited substrate availability secondary to impaired muscle perfusion. Therefore, we sought to elucidate the role of muscle perfusion on the age-related decline of muscle oxidative capacity and ultimately whole-body aerobic capacity. Muscle oxidative capacity was assessed by 31P-MRS post-exercise phosphocreatine recovery time (τPCr), with higher τPCr reflecting lower oxidative capacity, in 75 healthy participants (48 men, 22-89 years) of the Genetic and Epigenetic Signatures of Translational Aging Laboratory Testing study. Muscle perfusion was characterized as an index of blood volume at rest using a customized diffusion-weighted MRI technique and analysis method developed in our laboratory. Aerobic capacity (peak-VO2) was also measured during a graded treadmill exercise test in the same visit. Muscle oxidative capacity, peak-VO2, and resting muscle perfusion were significantly lower at older ages independent of sex, race, and body mass index (BMI). τPCr was significantly associated with resting muscle perfusion independent of age, sex, race, and BMI (p-value = 0.004, β = -0.34). τPCr was also a significant independent predictor of peak-VO2 and, in a mediation analysis, significantly attenuated the association between muscle perfusion and peak-VO2 (34% reduction for β in perfusion). These findings suggest that the age-associated decline in muscle oxidative capacity is partly due to impaired muscle perfusion and not mitochondrial dysfunction alone. Furthermore, our findings show that part of the decline in whole-body aerobic capacity observed with aging is also due to reduced microvascular blood volume at rest, representing a basal capacity of the microvascular system, which is mediated by muscle oxidative capacity. This finding suggests potential benefit of interventions that target an overall increase in muscle perfusion for the restoration of energetic capacity and mitochondrial function with aging
Estrogenic regulation of skeletal muscle proteome : a study of premenopausal women and postmenopausal MZ cotwins discordant for hormonal therapy
Female middle age is characterized by a decline in skeletal muscle mass and performance, predisposing women to sarcopenia, functional limitations, and metabolic dysfunction as they age. Menopausal loss of ovarian function leading to low circulating level of 17-estradiol has been suggested as a contributing factor to aging-related muscle deterioration. However, the underlying molecular mechanisms remain largely unknown and thus far androgens have been considered as a major anabolic hormone for skeletal muscle. We utilized muscle samples from 24 pre- and postmenopausal women to establish proteome-wide profiles, associated with the difference in age (30-34 years old vs. 54-62 years old), menopausal status (premenopausal vs. postmenopausal), and use of hormone replacement therapy (HRT; user vs. nonuser). None of the premenopausal women used hormonal medication while the postmenopausal women were monozygotic (MZ) cotwin pairs of whom the other sister was current HRT user or the other had never used HRT. Label-free proteomic analyses resulted in the quantification of 797 muscle proteins of which 145 proteins were for the first time associated with female aging using proteomics. Furthermore, we identified 17-estradiol as a potential upstream regulator of the observed differences in muscle energy pathways. These findings pinpoint the underlying molecular mechanisms of the metabolic dysfunction accruing upon menopause, thus having implications for understanding the complex functional interactions between female reproductive hormones and health.Peer reviewe
Eye gaze metrics reflect a shared motor representation for action observation and movement imagery
Action observation (AO) and movement imagery (MI) have been reported to share similar neural networks. This study investigated the congruency between AO and MI using the eye gaze metrics, dwell time and fixation number. A simple reach-grasp-place arm movement was observed and, in a second condition, imagined where the movement was presented from the first person perspective (1PP) and the third person perspective (3PP). Dwell time and number of fixations were calculated for whole scene and regions of interest (ROIs). For whole scene, no significant differences were found in the number of fixations for condition (AO, MI) or perspective. Dwell time, however, was significantly longer in AO than MI. For ROIs, the number of fixations was significantly greater in 1PP than 3PP. The data provide support for congruence between motor simulation states but also indicate some functional differences
Active Vision during Action Execution, Observation and Imagery: Evidence for Shared Motor Representations
The concept of shared motor representations between action execution and various covert conditions has been demonstrated through a number of psychophysiological modalities over the past two decades. Rarely, however, have
researchers considered the congruence of physical, imaginary and observed movement markers in a single paradigm and never in a design where eye movement metrics are the markers. In this study, participants were required to perform a forward reach and point Fitts’ Task on a digitizing tablet whilst wearing an eye movement system. Gaze metrics were used to compare behaviour congruence between action execution, action observation, and guided and unguided movement imagery conditions. The data showed that participants attended the same task-related visual cues between conditions but the strategy was different. Specifically, the number of fixations was significantly different between action execution and all covert conditions. In addition, fixation duration was congruent between action execution and action observation only, and
both conditions displayed an indirect Fitts’ Law effect. We therefore extend the understanding of the common motor representation by demonstrating, for the first time, common spatial eye movement metrics across simulation conditions
and some specific temporal congruence for action execution and action observation. Our findings suggest that action
observation may be an effective technique in supporting motor processes. The use of video as an adjunct to physical
techniques may be beneficial in supporting motor planning in both performance and clinical rehabilitation environments
Modulation of spinal excitability following neuromuscular electrical stimulation superimposed to voluntary contraction
Purpose. Neuromuscular electrical stimulation (NMES) superimposed on voluntary muscle contraction has been recently
shown as an innovative training modality within sport and rehabilitation, but its effects on the neuromuscular system are
still unclear. The aim of this study was to investigate acute responses in spinal excitability, as measured by the Hoffmann
(H) reflex, and in maximal voluntary contraction (MVIC) following NMES superimposed to voluntary isometric
contractions (NMES+ISO) compared to passive NMES only and to voluntary isometric contractions only (ISO). Method.
Fifteen young adults were required to maintain an ankle plantar-flexor torque of 20% MVC for 20 repetitions during each
experimental condition (NMES+ISO, NMES and ISO). Surface electromyography was used to record peak-to-peak Hreflex and motor waves following percutaneous stimulation of the posterior tibial nerve in the dominant limb. An
isokinetic dynamometer was used to assess maximal voluntary contraction output of the ankle plantar flexor muscles.
Results. H-reflex amplitude was increased by 4.5% after the NMES+ISO condition (p < 0.05), while passive NMES and
ISO conditions showed a decrease by 7.8% (p < 0.05) and no change in reflex responses, respectively. There was no
change in amplitude of maximal motor wave and in MVIC torque during each experimental condition. Conclusion. The
reported facilitation of spinal excitability following NMES+ISO could be due to a combination of greater motor neuronal
and corticospinal excitability, thus suggesting that NMES superimposed onto isometric voluntary contractions may
provide a more effective neuromuscular stimulus and, hence, training modality compared to NMES alone
Physical activity and exercise: Strategies to manage frailty
Frailty, a consequence of the interaction of the aging process and certain chronic diseases, compromises functional
outcomes in the elderly and substantially increases their risk for developing disabilities and other adverse
outcomes. Frailty follows from the combination of several impaired physiological mechanisms affecting multiple
organs and systems. And, though frailty and sarcopenia are related, they are two different conditions. Thus,
strategies to preserve or improve functional status should consider systemic function in addition to muscle
conditioning. Physical activity/exercise is considered one of the main strategies to counteract frailty-related
physical impairment in the elderly. Exercise reduces age-related oxidative damage and chronic inflammation,
increases autophagy, and improves mitochondrial function, myokine profile, insulin-like growth factor-1 (IGF-1)
signaling pathway, and insulin sensitivity. Exercise interventions target resistance (strength and power), aerobic,
balance, and flexibility work. Each type improves different aspects of physical functioning, though they could be
combined according to need and prescribed as a multicomponent intervention. Therefore, exercise intervention
programs should be prescribed based on an individual's physical functioning and adapted to the ensuing response.pre-print2.493 K
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