An Energy Potential Estimation Methodology and Novel Prototype Design for Building-Integrated Wind Turbines

ROSEO-BIWT is a new Building-Integrated Wind Turbine (BIWT) intended for installation on the edge of buildings. It consists of a Savonius wind turbine and guiding vanes to accelerate the usual horizontal wind, together with the vertical upward air stream on the wall. This edge effect improves the performance of the wind turbine, and its architectural integration is also beneficial. The hypothetical performance and design configuration were studied for a university building in Eibar city using wind data from the ERA5 reanalysis (European Centre for Medium-Range Weather Forecasts’ reanalysis), an anemometer to calibrate the data, and the actual small-scale behavior in a wind tunnel. The data acquired by the anemometer show high correlations with the ERA5 data in the direction parallel to the valley, and the calibration is therefore valid. According to the results, a wind speed augmentation factor of three due to the edge effect and concentration vanes would lead to a increase in working hours at the rated power, resulting annually in more than 2000 h.The research leading to these results was carried out in the framework of the Programme Campus Bizia Lab EHU (Campus Living Lab) with a financial grant from the Office of Sustainability of the Vice-Chancellorship for Innovation, Social Outreach and Cultural Activities of the University of the Basque Country (UPV/EHU). This programme is supported by the Basque Government. We acknowledge also the availability given by the School of Engineering of Gipuzkoa-Eibar in the University of Basque Country, the EDP-Renewable awards in which we obtained the main award in September 2017, the Youth Enterprise Grant of UPV/EHU, and the project GIU17/02 of EHU/UPV. All computations and representations of this work were developed using the programming language

Detection of protection benefits for predatory fishes depends on census methodology

Marine protected areas (MPAs) are used as fisheries management and conservation tools. Well-enforced no-take zones allow the rebuilding of natural populations of exploited species; however, there is still controversy on the role of buffer zones. The effectiveness of MPAs could be underestimated, as fish population assessments depend largely on traditional methodologies that have difficulties in detecting predatory fish because of their low abundances, their patchy distribution, and their reaction to the presence of divers. The performance of different census methods was compared in assessing the protection benefits for large predatory fishes under different protection levels (i.e. no-take and buffer zones) in five Mediterranean MPAs. Specifically, conventional strip transects (CSTs, 50 × 5 m2) and tracked roaming transects combined with distance sampling (TRT + DS, variable lengths) were compared, including a series of TRT-derived estimators with variable transect lengths and fixed widths of 20, 10, and 6 m (TRT20, TRT10, and TRT6, respectively). Additionally, the effectiveness of the MPAs studied and protection levels for conserving large predatory species was evaluated. Transects covering larger areas (i.e. TRT + DS and TRT20) allowed the detection of a greater number of species and yielded more accurate estimates of density and biomass than transects of narrower fixed widths, particularly the CSTs, which were associated with the lowest richness detection capability, accuracy, and precision. On average, both no-take zones and buffer zones appeared effective for the conservation of predatory fishes, indicating that multiple protection areas were ecologically effective. Differences between MPAs were also observed, however, probably arising from both local environmental and management factors. We suggest the implementation of methodologies with larger transects for the study of large predatory fish, combined with CSTs for the rest of the fish community, in order to avoid biases in predatory population assessments, which are key indicators of MPA effectiveness

A video guide of five access methods to the splenic flexure: the concept of the splenic flexure box

Aim: The aim of this study was to describe all the possible approaches for laparoscopic splenic flexure mobilization (SFM), each suitable for specific situations, and create an illustrated system to show SFM approaches in an easy and practical way to make it easy to learn and teach. Methods: Two different phases. First part: Cadaver-based study of the colonic splenic flexure anatomy. In order to demonstrate the different approaches, a balloon was placed through the colonic hepatic flexure in the lesser sac without sectioning any of the fixing ligaments of the splenic flexure. Second part: A real case series of laparoscopic SFM. Results: First part: 11 cadavers were dissected. Five potential approaches to SFM were found: anterior, trans-omentum, lateral, medial infra-mesocolic, and medial trans-mesocolic. The illustrative system developed was named: Splenic Flexure “Box”(SFBox). Second part: One of the types of SFM described in first part was used in five patients with colorectal cancer. Each laparoscopic approach to the splenic flexure was illustrated in a video accompanied by illustration aids delineating the access. Conclusion: With the cadaver dissection and subsequent demonstration in real-life laparoscopic surgery, we have shown five types of laparoscopic splenic flexure mobilization. The Splenic Flexure “Box” is a useful way to learn and teach this surgical maneuver

Variability of CD3 membrane expression and T cell activation capacity

αβT cells have a wide distribution of their CD3 membrane density. The aim of this paper was to evaluate the significance of the CD3 differential expression on T cell subsets. Analysis was performed on healthy donors and renal transplant patients by flowcytometry The results obtained are : 1-CD3 expression was widely distributed (CV =38.3±3.1 to (43±2.3%). 2-The CD4, CD8,CD45 and forward scatter were similarly distributed. 3-The diversity of CD3 expression was direcly related to the clonotypes: γ9, non γ9 from γδT cells and Vβ clonotype from αβT cells (e.g.: Vβ3FITC 7980±1628 Vβ8PE: Vβ20-FITC 11768±1510). 4-Using a computer simulation, we could confirm differential kinetics of T cell activation according to the initial parameters. Finally, in vitro activation was significantly higher on Vβ8 and Vβ9 (high CD3) compared to Vβ2 and Vβ3 (low CD3, P=0.040 to 0.0003). In conclusion: T cells have highly heterogeneous CD3 expression, possibly predetermined and with clear functional significance

Fgf10(+) progenitors give rise to the chick hypothalamus by rostral and caudal growth and differentiation

Classical descriptions of the hypothalamus divide it into three rostro-caudal domains but little is known about their embryonic origins. To investigate this we performed targeted fate-mapping, molecular characterisation and cell cycle analyses in the embryonic chick. Presumptive hypothalamic cells derive from the rostral diencephalic ventral midline, lie above the prechordal mesendoderm and express Fgf10Fgf10(+) progenitors undergo anisotropic growth: those displaced rostrally differentiate into anterior cells, then those displaced caudally differentiate into mammillary cells. A stable population of Fgf10(+) progenitors is retained within the tuberal domain, a subset of these give rise to the tuberal infundibulum, the precursor of the posterior pituitary. Pharmacological approaches reveal that Shh signalling promotes the growth and differentiation of anterior progenitors and also orchestrates the development of the infundibulum and Rathke's pouch, the precursor of the anterior pituitary. Together our studies identify a hypothalamic progenitor population defined by Fgf10 and highlight a role for Shh signalling in the integrated development of the hypothalamus and pituitary

Dendritic Cells Exposed to MVA-Based HIV-1 Vaccine Induce Highly Functional HIV-1-Specific CD8+ T Cell Responses in HIV-1-Infected Individuals

Currently, MVA virus vectors carrying HIV-1 genes are being developed as HIV-1/AIDS prophylactic/therapeutic vaccines. Nevertheless, little is known about the impact of these vectors on human dendritic cells (DC) and their capacity to present HIV-1 antigens to human HIV-specific T cells. This study aimed to characterize the interaction of MVA and MVA expressing the HIV-1 genes Env-Gag-Pol-Nef of clade B (referred to as MVA-B) in human monocyte-derived dendritic cells (MDDC) and the subsequent processes of HIV-1 antigen presentation and activation of memory HIV-1-specific T lymphocytes. For these purposes, we performed ex vivo assays with MDDC and autologous lymphocytes from asymptomatic HIV-infected patients. Infection of MDDC with MVA-B or MVA, at the optimal dose of 0.3 PFU/MDDC, induced by itself a moderate degree of maturation of MDDC, involving secretion of cytokines and chemokines (IL1-ra, IL-7, TNF-α, IL-6, IL-12, IL-15, IL-8, MCP-1, MIP-1α, MIP-1β, RANTES, IP-10, MIG, and IFN-α). MDDC infected with MVA or MVA-B and following a period of 48 h or 72 h of maturation were able to migrate toward CCL19 or CCL21 chemokine gradients. MVA-B infection induced apoptosis of the infected cells and the resulting apoptotic bodies were engulfed by the uninfected MDDC, which cross-presented HIV-1 antigens to autologous CD8+ T lymphocytes. MVA-B-infected MDDC co-cultured with autologous T lymphocytes induced a highly functional HIV-specific CD8+ T cell response including proliferation, secretion of IFN-γ, IL-2, TNF-α, MIP-1β, MIP-1α, RANTES and IL-6, and strong cytotoxic activity against autologous HIV-1-infected CD4+ T lymphocytes. These results evidence the adjuvant role of the vector itself (MVA) and support the clinical development of prophylactic and therapeutic anti-HIV vaccines based on MVA-B

Association of Candidate Gene Polymorphisms With Chronic Kidney Disease: Results of a Case-Control Analysis in the Nefrona Cohort

Chronic kidney disease (CKD) is a major risk factor for end-stage renal disease, cardiovascular disease and premature death. Despite classical clinical risk factors for CKD and some genetic risk factors have been identified, the residual risk observed in prediction models is still high. Therefore, new risk factors need to be identified in order to better predict the risk of CKD in the population. Here, we analyzed the genetic association of 79 SNPs of proteins associated with mineral metabolism disturbances with CKD in a cohort that includes 2, 445 CKD cases and 559 controls. Genotyping was performed with matrix assisted laser desorption ionizationtime of flight mass spectrometry. We used logistic regression models considering different genetic inheritance models to assess the association of the SNPs with the prevalence of CKD, adjusting for known risk factors. Eight SNPs (rs1126616, rs35068180, rs2238135, rs1800247, rs385564, rs4236, rs2248359, and rs1564858) were associated with CKD even after adjusting by sex, age and race. A model containing five of these SNPs (rs1126616, rs35068180, rs1800247, rs4236, and rs2248359), diabetes and hypertension showed better performance than models considering only clinical risk factors, significantly increasing the area under the curve of the model without polymorphisms. Furthermore, one of the SNPs (the rs2248359) showed an interaction with hypertension, being the risk genotype affecting only hypertensive patients. We conclude that 5 SNPs related to proteins implicated in mineral metabolism disturbances (Osteopontin, osteocalcin, matrix gla protein, matrix metalloprotease 3 and 24 hydroxylase) are associated to an increased risk of suffering CKD

Mechanistic Investigations into the Application of Sulfoxides in Carbohydrate Synthesis

The utility of sulfoxides in a diverse range of transformations in the field of carbohydrate chemistry has seen rapid growth since the first introduction of a sulfoxide as a glycosyl donor in 1989. Sulfoxides have since developed into more than just anomeric leaving groups, and today have multiple roles in glycosylation reactions. These include as activators for thioglycosides, hemiacetals, and glycals, and as precursors to glycosyl triflates, which are essential for stereoselective β- mannoside synthesis, and bicyclic sulfonium ions that facilitate the stereoselective synthesis of α-glycosides. In this review we highlight the mechanistic investigations undertaken in this area, often outlining strategies employed to differentiate between multiple proposed reaction pathways, and how the conclusions of these investigations have and continue to inform upon the development of more efficient transformations in sulfoxide based carbohydrate synthesis