Sorption properties of light hydrocarbon-contaminated clay under defined conditions

Abstract Risk assessments and remediation plans of hydrocarbon-contaminated sites usually have been managed based on total petroleum hydrocarbon (TPH) content. Gasoline is one of the most mobile pollution agents, which consist mainly of light hydrocarbons. Measuring of TPH does not give enough information about the individual compounds. Another problem is using solvents such as n-hexane for extraction. In some cases we would like to know about the hydrocarbon-adsorption properties of contaminated samples. The article describes a laboratory model for hydrocarbon contamination modeling, and measuring hydrocarbon sorption properties. For modeling the authors have chosen gasoline type hydrocarbon, homogeneous sand and clay samples from the Kiscell Clay Formation. Results of the modeling indicate that the referenced ATD-GC/MS method can be a very effective tool in the risk assessment of hydrocarbon-contaminated areas

Protection of aouts monkeys after immunization with recombinant antigens of Plasmodium falciparum

The genus Aotus spp. (owl monkey) is one of the WHO recommended experimental models for Plasmodium falciparum blood stage infection, especially relevant for vaccination studies with asexual blood stage antigens of this parasite. For several immunization trials with purified recombinant merozoite/schizont antigens, the susceptible Aouts kenotypes II, III, IV and VI were immunized with Escherichia coli derived fusion proteins containg partial sequences of the proteins MSAI (merozoite surface antigen I), SERP (serine-strech protein) and HRPII (histidine alanine rich protein II) as well as with a group of recombinant antigens obtained by an antiserum raised against a protective 41 kD protein band. The subcutaneous application (3x) of the antigen preparations was carried out in intact animals followed by splenectomy prior to challange, in order to increase the susceptibility of the experimental hosts to the parasite. A partial sequence of HRPII, the combination of three different fusion proteins of the 41 kD group and mixture of two sequences of SERP in the presence of the modified Al(OH)3 adjuvant conferred significant protection against a challange infection with P. falciparum blood stages (2-5 x 10 (elevado a sexta potência) i. RBC). Monkey immunized with the MS2-fusion protein carrying the N-terminal part of the 195 kD precursor of the major merozoite surface antigens induced only marginal protection showing some correlation between antibody titer and degree of parasitaemia. Based on the protective capacity of these recombinant antigens we have expressed two hybrid proteins (MS2/SERP/HRPII and SERP/MSAI/HRPII) in E. coli containing selected partial sequences of SERP, HRPII and MSAI. Antibodies raised against both hybrid proteins in rabbits and Aotus monkeys recognize the corresponding schizont polypeptides. In two independent immunization trials using 13 animals (age 7 months to 3 years) we could show that immunization of Aotus monkeys with either of the two hybrid proteins administered in an oil-based well tolerated formulation protected the animals frm a severe experimental P. falciparum (strain Palo Alto) infection

Examining the Attitudes and Knowledge of Pregnant Teens on the Topic of SIDS

Background: Norfolk has the second highest teen pregnancy rate and third highest infant mortality rate in Southeastern Virginia. SIDS is the third leading cause of infant death in Virginia. Providing a group education intervention modeled after centering pregnancy gives teen mothers the opportunity to learn and receive support in a safe space in hopes of making a positive impact on their attitudes and knowledge regarding SIDS. Hypothesis: Do the attitudes and knowledge of pregnant teens and recent teen mothers change positively after a group education intervention on sudden infant death syndrome (SIDS). Method: Quasi-experimental non-randomized group trial. This includes pregnant females ages fifteen to nineteen in Norfolk, Virginia at a city general hospital. The data will be collected from a pretest before and posttest after a group education intervention on SIDS. Data Collection: Hypothesis testing will be conducted using a t-test to determine group differences in attitudes and knowledge after the intervention. Participants will also be completing a PRAMS demographics survey. Goal: To see if the SIDS education intervention has an impact on the knowledge of teen mothers in efforts to reduce the current SIDS rate contributing to Norfolk\u27s high infant mortality rate

Race and gender-based perceptions of older septuagenarian adults

OBJECTIVES: Older adults face racism, sexism, and ageism. As the U.S. population ages, it is important to understand how the current population views older adults. METHODS: Participants recruited through Amazon\u27s Mechanical Turk provided perceptions of older Black and White models\u27 photographs. Using mixed-effect models, we assessed interactions between race and gender of participants and models. RESULTS: Among Participants of Color and White participants ( DISCUSSION: Participants had few biases toward older Black and White models, while gender biases favored men

allodb: An R package for biomass estimation at globally distributed extratropical forest plots

Allometric equations for calculation of tree above-ground biomass (AGB) form the basis for estimates of forest carbon storage and exchange with the atmosphere. While standard models exist to calculate forest biomass across the tropics, we lack a standardized tool for computing AGB across boreal and temperate regions that comprise the global extratropics. Here we present an integrated R package, allodb, containing systematically selected published allometric equations and proposed functions to compute AGB. The data component of the package is based on 701 woody species identified at 24 large Forest Global Earth Observatory (ForestGEO) forest dynamics plots representing a wide diversity of extratropical forests. A total of 570 parsed allometric equations to estimate individual tree biomass were retrieved, checked and combined using a weighting function designed to ensure optimal equation selection over the full tree size range with smooth transitions across equations. The equation dataset can be customized with built-in functions that subset the original dataset and add new equations. Although equations were curated based on a limited set of forest communities and number of species, this resource is appropriate for large portions of the global extratropics and can easily be expanded to cover novel forest types

Revising mtDNA haplotypes of the ancient Hungarian conquerors with next generation sequencing

As part of the effort to create a high resolution representative sequence database of the medieval Hungarian conquerors we have resequenced the entire mtDNA genome of 24 published ancient samples with Next Generation Sequencing, whose haplotypes had been previously determined with traditional PCR based methods. We show that PCR based methods are prone to erroneous haplotype or haplogroup determination due to ambiguous sequence reads, and many of the resequenced samples had been classified inaccurately. The SNaPshot method applied with published ancient DNA authenticity criteria is the most straightforward and cheapest PCR based approach for testing a large number of coding region SNP-s, which greatly facilitates correct haplogroup determination

Search for supersymmetry in events with one lepton and multiple jets in proton-proton collisions at root s=13 TeV

Peer reviewe