Expression of CD90 and P75NTR stem cell markers in ameloblastomas : a possible role in their biological behavior

Multicystic and unicystic ameloblastomas are benign odontogenic tumors that present distinct biological behavior. The investigation of stem cells has become an important branch of tumor biology, with several studies addressing the possible role of these cells in tumor growth, angiogenesis, progression, infiltration and invasiveness. This study evaluated the immunohistochemical expression of CD90(Thy-1) and P75NTR stem cell markers in multicystic and unicystic ameloblastomas. Seventeen (17) samples of ameloblastomas (multicystic, n = 10; unicystic, n = 7) were submitted to immunohistochemical reactions and graded semi-quantitatively. The Kolmogorov-Smirnov test was used to verify possible differences in CD90 and P75NTR expressions between multicystic and unicystic ameloblastomas (p < 0.05). CD90 immunostaining was observed in all multicystic ameloblastoma specimens (n = 10), in the cytoplasm of the fibroblasts and vascular endothelial cells of the tumor stroma, near the neoplastic odontogenic epithelia. The staining of stromal CD90 was significantly higher in multicystic than in unicystic ameloblastomas (p = 0.003). Nuclear P75NTR immunostaining was observed in all ameloblastoma specimens. A significant difference was seen in the epithelial staining of P75NTR between multicystic and unicystic types (p = 0.007). The increased expression of CD90 and P75NTR found in multicystic ameloblastomas suggests a behavioral biological difference between multicystic and unicystic ameloblastomas, as well as a difference in ameloblastoma development

Experimental behavior of masonry wall-to-timber elements connections strengthened with injection anchors

Out-of-plane failure mechanisms observed in stone masonry buildings subjected to seismic action are often a direct result of poor connections between structural elements. During a seismic event these weak connections become incapable of assuring proper load transmission. Therefore, the need to prevent these phenomena is of critical importance in understanding the behavior of unstrengthened masonry buildings along with the necessity of developing effective strengthening solutions. This paper presents injection anchors as a viable option to improve anchorage between masonry and timber elements on historical buildings, as for example wall-to-timber framed wall or wall-to-timber diaphragm connections. The experimental campaign consisted of quasi-static monotonic and cyclic pullout tests performed on real scale specimens, representative of wall-to-timber framed wall connections found in late 19th century buildings of downtown Lisbon, Portugal. Combined cone-bond failure was obtained in all 7 tests. Boundary conditions of the specimens greatly affected the results in terms of maximum pullout force, dissipated energy, and strength degradation. Displacement ductility of the strengthened connections is high. The force-displacement curves clearly pointed out the influence on the results of the wall's compressive stress state and the contribution of friction in the grout/masonry interface.This work was partially funded by project FP7-ENV-2009-1-244123-NIKER of the 7th Framework Program of the European Commission, which is gratefully acknowledged. Authors would like to thank the technical staff of the Structures Lab. of University of Minho for the assistance provided preparing and carrying out the tests and also, Monumenta, Ltd. for the construction of the masonry walls and Cintec (R) for the installation of the injection anchors

Decision-making of English Netball Superleague umpires: Contextual and dispositional influences

Objectives. The decisions made by officials have a direct bearing on the outcomes of competitive sport contests. In an exploratory study, we examine the interrelationships between the decisions made by elite netball umpires, the potential contextual and environmental influences (e.g., crowd size), and the umpires’ dispositional tendencies – specifically, their propensity to deliberate and ruminate on their decisions. Design/Method. Filmed footage from 60 England Netball Superleague matches was coded using performance analysis software. We measured the number of decisions made overall, and for home and away teams; league position; competition round; match quarter; and crowd size. Additionally, 10 umpires who officiated in the matches completed the Decision-Specific Reinvestment Scale (DSRS). Results. Regression analyses predicted that as home teams’ league position improved the number of decisions against away teams increased. A model comprising competition round and average league position of both teams predicted the number of decisions made in matches, but neither variable emerged as a significant predictor. The umpire analyses revealed that greater crowd size was associated with an increase in decisions against away teams. The Decision Rumination factor was strongly negatively related to the number of decisions in Quarters 1 and 3, this relationship was driven by fewer decisions against home teams by umpires who exhibited higher Rumination subscale scores. Conclusions. These findings strengthen our understanding of contextual, environmental, and dispositional influences on umpires’ decision-making behaviour. The tendency to ruminate upon decisions may explain the changes in decision behaviour in relation to the home team advantage effect

Acquired resistance to oxaliplatin is not directly associated with increased resistance to DNA damage in SK-N-ASrOXALI4000, a newly established oxaliplatin-resistant sub-line of the neuroblastoma cell line SK-N-AS

The formation of acquired drug resistance is a major reason for the failure of anti-cancer therapies after initial response. Here, we introduce a novel model of acquired oxaliplatin resistance, a sub-line of the non-MYCN-amplified neuroblastoma cell line SK-N-AS that was adapted to growth in the presence of 4000 ng/mL oxaliplatin (SK-N-ASrOXALI4000). SK-N-ASrOXALI4000 cells displayed enhanced chromosomal aberrations compared to SK-N-AS, as indicated by 24-chromosome fluorescence in situ hybridisation. Moreover, SK-N-ASrOXALI4000 cells were resistant not only to oxaliplatin but also to the two other commonly used anti-cancer platinum agents cisplatin and carboplatin. SK-N-ASrOXALI4000 cells exhibited a stable resistance phenotype that was not affected by culturing the cells for 10 weeks in the absence of oxaliplatin. Interestingly, SK-N-ASrOXALI4000 cells showed no cross resistance to gemcitabine and increased sensitivity to doxorubicin and UVC radiation, alternative treatments that like platinum drugs target DNA integrity. Notably, UVC-induced DNA damage is thought to be predominantly repaired by nucleotide excision repair and nucleotide excision repair has been described as the main oxaliplatin-induced DNA damage repair system. SK-N-ASrOXALI4000 cells were also more sensitive to lysis by influenza A virus, a candidate for oncolytic therapy, than SK-N-AS cells. In conclusion, we introduce a novel oxaliplatin resistance model. The oxaliplatin resistance mechanisms in SK-N-ASrOXALI4000 cells appear to be complex and not to directly depend on enhanced DNA repair capacity. Models of oxaliplatin resistance are of particular relevance since research on platinum drugs has so far predominantly focused on cisplatin and carboplatin

Bridging gaps in image meme research: A multidisciplinary paradigm for scaling up qualitative analyses

This paper outlines a multidisciplinary framework (Digital Rhetorical Ecosystem or DRE3) for scaling up qualitative analyses of image memes. First, we make a case for applying rhetorical theory to examine image memes as quasi-arguments that promote claims on a variety of political and social issues. Next, we argue for integrating rhetorical analysis of image memes into an ecological framework to trace interaction and evolution of memetic claims as they coalesce into evidence ecosystems that inform public narratives. Finally, we apply a computational framework to address the particular problem of claim identification in memes at large scales. Our integrated framework answers the recent call in information studies to highlight the social, political, and cultural attributes of information phenomena, and bridges the divide between small-scale qualitative analyses and large-scale computational analyses of image memes. We present this theoretical framework to guide the development of research questions, processes, and computational architecture to study the widespread and powerful influence of image memes in shaping consequential public beliefs and sentiments

DISTINCTION BETWEEN COLLEGE STUDENT FROM DIFFERENT COUNTRIES ASSOCIATED TO HOPE, OPTIMISM AND RESILIENCE

The article, a partial result of an international cross-sectional research, aims to introduce the differences between the college students from the participating countries associated to the hope and optimism feelings and the resilient behavior. The data was obtained through a transversal research with virtual data collection in the year of 2019 having the participation of n = 1112 students from n = 10 countries. There were found important distinctions between the countries about the hope and optimism feeling and the resilient behavior, p = 0.000. Further studies on the subject may provide more knowledge about the phenomenon. Keyword: College Student. Psychology of Upper Education. Hope. Optimism. Resilience.O artigo, resultado parcial de pesquisa internacional de cunho transversal, tem como objetivo apresentar diferenças entre estudantes universitários dos países participantes associadas aos sentimentos de esperança e otimismo e ao comportamento resiliente. Os dados foram obtidos por meio de pesquisa transversal com coleta de dados via digital em 2019 contando com participação de n=1112 estudantes de n=10 países. Foram encontradas diferenças significativas entre os países para os sentimentos de otimismo e esperança e comportamento resiliente p=0,000. A realização de outros estudos sobre o tema poderão aportar mais conhecimento sobre o fenômeno. &nbsp;Palavras-chave: Estudantes universitários.&nbsp; Psicologia do Ensino superior. Esperança, Otimismo. Resiliência

Genome-wide analysis identifies 12 loci influencing human reproductive behavior.

The genetic architecture of human reproductive behavior-age at first birth (AFB) and number of children ever born (NEB)-has a strong relationship with fitness, human development, infertility and risk of neuropsychiatric disorders. However, very few genetic loci have been identified, and the underlying mechanisms of AFB and NEB are poorly understood. We report a large genome-wide association study of both sexes including 251,151 individuals for AFB and 343,072 individuals for NEB. We identified 12 independent loci that are significantly associated with AFB and/or NEB in a SNP-based genome-wide association study and 4 additional loci associated in a gene-based effort. These loci harbor genes that are likely to have a role, either directly or by affecting non-local gene expression, in human reproduction and infertility, thereby increasing understanding of these complex traits

Decoding Lua: formal semantics for the developer and the semanticist

We provide formal semantics for a large subset of the Lua programming language, in its version 5.2. We validate our model by mechanizing it and testing it against the test suite of the reference interpreter of Lua, con rming that our model accurately represents the language. In addition, we set us an ambitious goal: to target both a PL semanticist —not necessarily versed in Lua—, and a Lua developer —not necessarily versed in semantic frameworks. To the former, we present the peculiarities of the language, and how we model them in a traditional small-step operational semantics, embedded within Felleisen-Hieb’s reduction semantics with evaluation contexts. e mechanization is, naturally, performed in PLT Redex, the de facto tool for mechanizing reduction semantics. To the reader unfamiliar with such concepts, we provide, to our best possible within the space limitations, a gentle introduction of the model. It is our hope that developers of the di erent Lua implementations and dialects understand the model and consider it both for testing their work and for experimenting with new language features.Fil: Soldevila Raffa, Mallku Ernesto. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Ziliani, Beta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Silvestre, Bruno. Universidade Federal de Goiás; BrasilFil: Fridlender, Daniel Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Mascarenhas, Fabio. Universidade Federal do Rio de Janeiro; Brasi

Elective Discontinuation of Larotrectinib in Pediatric Patients With TRK Fusion Sarcomas and Related Mesenchymal Tumors

Pediatric patients; Sarcomas; Mesenchymal tumorsPacients pediàtrics; Sarcomes; Tumors mesenquimalsPacientes pediátricos; Sarcomas; Tumores mesenquimalesLarotrectinib is a highly selective tropomyosin receptor kinase (TRK) inhibitor with efficacy in children with TRK fusion tumors. We evaluated patient outcomes after elective discontinuation of larotrectinib in the absence of disease progression in a protocol-defined wait-and-see subset analysis of eligible patients where treatment resumption with larotrectinib was allowed if disease progressed. We also assessed the safety and efficacy of larotrectinib in all pediatric patients with sarcoma. This cohort included 91 patients (younger than 18 years) from two clinical trials: infantile fibrosarcoma (49), other soft tissue sarcomas or related mesenchymal tumors (41), and bone sarcoma (1). Treatment-related adverse events were of maximum grade 1 or 2 in 25% and 25% of patients, respectively. The overall response rate was 87% (95% CI, 78 to 93). In the wait-and-see analysis, 47 patients discontinued larotrectinib. Median time from discontinuation to disease progression was not reached. Sixteen patients had tumor progression during the wait-and-see period. All 16 patients resumed larotrectinib, and 15 (94%) achieved disease control, with 11 objective responses. Larotrectinib continues to demonstrate durable responses with favorable safety in children with TRK fusion sarcomas. Treatment discontinuation is feasible in select patients with objective response and clinical benefit noted in those who have disease progression after elective treatment discontinuation.Supported by Bayer Healthcare and Loxo Oncology, Inc, a wholly owned subsidiary of Eli Lilly and Company. S.G.D. was supported by Alex's Lemonade Stand Foundation. J.C. was supported by the Giant Pledge through the Royal Marsden Cancer Charity, and this work represents independent research supported by the National Institute for Health Research Biomedical Research Center at The Royal Marsden NHS Foundation Trust and the Institute of Cancer Research, London

Rising water flow as a factor of organic material importation into caves

The Carlúcio Cave is located on the left bank of the Peruaçu River canyon, in a National Park located in the semiarid zone of Minas Gerais, Southeastern Brazil. Although the Carlúcio Cave is in an unfavorable relief position in terms of material importation into its interior, it harbors an important fossil deposit. This paper sought to investigate such fossil assemblage and describe material importation and deposition events. For that purpose, a survey of the existing fossils was conducted, including a description of the deposition sites and taphonomic signatures. A topographic survey was carried out of representative samples and the deposit chronology was established by means of carbon-14 and uranium-series dating. The fossil assemblage comprises mainly terrestrial epigeal mollusk shells, totaling 1,399 individuals, 70% of which are Drymaeus sp. (Bulimuloidea, Bulimulidae). In addition, 20 bones, 57 vegetal deposition sites, four charcoals, one palm fruit and one corncob were recorded. The cave location and morphology and the deposit’s taphonomic signature indicate that the material was carried upward by the rise in water level (WL) of the Peruaçu River due to episodic barriers to the natural river flow, followed by floating and retention of the material once the WL went gradually down. At least four flood events that reached the Carlúcio Cave were identified in the Peruaçu River canyon during the Holocene. This study provides evidence of a mechanism of material transport and accumulation in the cave caused by the rising water flow in connection with flood events in the river canyon.Key words: Cave deposits, Holocene chronology, fluvial geomorphology, Peruaçu River canyon.Naraščanje nivoja vode kot faktor vnosa organskega materiala v jameJama Carlúcio je na levem bregu kanjona reke Peruaçu v nacionalnem parku na polpuščavskem območju province Minas Gerais v jugovzhodni Braziliji. Čeprav je jama v neugodnem reliefnem položaju glede vnosa materiala, v njej ležijo pomembne fosilne najdbe. Ta članek je poskušal raziskati fosile in opisati dogodke vnosa in odlaganja materiala. Zato smo pregledali obstoječe fosile ter med drugim opisali odlagališča in tafonomske podpise. Izvedli smo topografsko raziskavo reprezentativnih vzorcev ter z določanjem starosti z ogljikom C14 in uranovo serijo vzpostavili depozitno kronologijo. Fosilni ostanki obsegajo predvsem kopenske epigealne mehkužce s 1399 posamezniki, od tega 70 % Drymaeus sp. (Bulimuloidea, Bulimulidae). Poleg tega smo opazili 20 kosti, 57 rastlinskih tališč, štiri oglja, eno palmovo sadje in eno koruzo. Lokacija jame, njena morfologija in tafonomske raziskave sedimentov nakazujejo, da je material odneslo navzgor s porastom nivoja vode reke Peruaçu zaradi epizodnih ovir pri naravnem toku reke. Temu sledi lebdenje in zadrževanje snovi, ko je nivo postopoma upadel. V holocenu smo v kanjonu reke Peruaçu identificirali vsaj štiri poplavne dogodke, ki so segli v jamo Carlúcio. Ta študija dokazuje mehanizem materialnega transporta in akumulacije v jami, ki jo povzroča naraščajoči nivo vode v povezavi s poplavnimi dogodki v rečnem kanjonu.Ključne besede: jamski sedimenti, holocenska kronologija, rečna geomorfologija, kanjon reke Peruçu