Charge distribution in two-dimensional electrostatics

We examine the stability of ringlike configurations of N charges on a plane interacting through the potential $V(z_1,...,z_N)=\sum_i |z_i|^2-\sum_{i<j} ln|z_i-z_j|^2$. We interpret the equilibrium distributions in terms of a shell model and compare predictions of the model with the results of numerical simulations for systems with up to 100 particles.Comment: LaTe

Enrichment and aggregation of topological motifs are independent organizational principles of integrated interaction networks

Topological network motifs represent functional relationships within and between regulatory and protein-protein interaction networks. Enriched motifs often aggregate into self-contained units forming functional modules. Theoretical models for network evolution by duplication-divergence mechanisms and for network topology by hierarchical scale-free networks have suggested a one-to-one relation between network motif enrichment and aggregation, but this relation has never been tested quantitatively in real biological interaction networks. Here we introduce a novel method for assessing the statistical significance of network motif aggregation and for identifying clusters of overlapping network motifs. Using an integrated network of transcriptional, posttranslational and protein-protein interactions in yeast we show that network motif aggregation reflects a local modularity property which is independent of network motif enrichment. In particular our method identified novel functional network themes for a set of motifs which are not enriched yet aggregate significantly and challenges the conventional view that network motif enrichment is the most basic organizational principle of complex networks.Comment: 12 pages, 5 figure

A quantitative polymerase chain reaction-enzyme immunoassay for accurate measurements of human papillomavirus type 16 DNA levels in cervical scrapings

A quantitative polymerase chain reaction-enzyme immunoassay (Q-PCR-EIA) was developed to measure the amount of human papillomavirus (HPV) 16 DNA per genome equivalent in cervical scrapings. The quantitative approach was based on a combined competitive PCR for both HPV 16, using the general primer GP5+/6+ PCR, and β-globin DNA. The two competitive PCRs involve co-amplification of target sequences and exogenously added DNA constructs carrying a rearranged 30 bp sequence in the probe-binding region. The accuracy of quantification by combining the two competitive PCR assays was validated on mixtures of HPV 16 containing cervical cancer cells of CaSki and SiHa cell lines. Comparison of this fully quantitative PCR assay with two semi-quantitative HPV PCR assays on a series of crude cell suspensions from HPV 16 containing cervical scrapings revealed remarkable differences in the calculated relative HPV load between samples. We found evidence that correction for both intertube variations in PCR efficiency and number of input cells/integrity of DNA significantly influence the outcome of studies on viral DNA load in crude cell suspensions of cervical scrapings. Therefore, accurate measurements on viral DNA load in cervical scrapings require corrections for these phenomena, which can be achieved by application of this fully quantitative approach. © 1999 Cancer Research Campaig

Mutations and Deregulation of Ras/Raf/MEK/ERK and PI3K/PTEN/Akt/mTOR Cascades Which Alter Therapy Response

The Ras/Raf/MEK/ERK and PI3K/PTEN/Akt/mTOR cascades are often activated by genetic alterations in upstream signaling molecules such as receptor tyrosine kinases (RTK). Certain components of these pathways, RAS, NF1, BRAF, MEK1, DUSP5, PP2A, PIK3CA, PIK3R1, PIK3R4, PIK3R5, IRS4, AKT, NFKB1, MTOR, PTEN, TSC1, and TSC2 may also be activated/inactivated by mutations or epigenetic silencing. Upstream mutations in one signaling pathway or even in downstream components of the same pathway can alter the sensitivity of the cells to certain small molecule inhibitors. These pathways have profound effects on proliferative, apoptotic and differentiation pathways. Dysregulation of components of these cascades can contribute to: resistance to other pathway inhibitors, chemotherapeutic drug resistance, premature aging as well as other diseases. This review will first describe these pathways and discuss how genetic mutations and epigenetic alterations can result in resistance to various inhibitors

Ejercicio de actualización de objetivos y metas

76 páginas.El presente ejercicio ha sido diseñado con base en un proyecto real, que fue formulado y ejecutado en un país latinoamericano, y ha sido estructurado de tal manera, que presenta una descripción amplia y con relativo nivel de detalle, de un proyecto hipotético que fue formulado años antes del momento en que se iniciara la ejecución. Su objetivo es lograr que los participantes consoliden los fundamentos conceptuales y metodológicos relativos a la fase del validación, del proceso de manejo de programas y proyectos, mediante la aplicación práctica de los mismos a una situación hipotética. Al finalizar el ejercicio, los participantes deberán haber analizado el documento del proyecto Tecnología apropiada para pequeños productores, identificado y reajustado los elementos centrales de la estrategia del mismo y elaborado una matriz de objetivos, con base en la situación descrita en el resumen del diagnóstico y las respectivas redes de pertinencia. Finalmente, se anexa el resumen de un diagnóstico actualizado, una lista de problemas relevantes del mismo y las perspectivas redes de pertinencia, elementos que serán de utilidad para el desarrollo del ejercicio

Alden Park Manor

Detached apartment buildings in garden setting (in contrast to earlier courtyard apartment buildings).general view, driveway, March 198

Alden Park Manor

Detached apartment buildings in garden setting (in contrast to earlier courtyard apartment buildings).general view, entrance, March 198