146 research outputs found
Evidence from GC-TRFLP that Bacterial Communities in Soil Are Lognormally Distributed
The Species Abundance Distribution (SAD) is a fundamental property of ecological communities and the form and formation of SADs have been examined for a wide range of communities including those of microorganisms. Progress in understanding microbial SADs, however, has been limited by the remarkable diversity and vast size of microbial communities. As a result, few microbial systems have been sampled with sufficient depth to generate reliable estimates of the community SAD. We have used a novel approach to characterize the SAD of bacterial communities by coupling genomic DNA fractionation with analysis of terminal restriction fragment length polymorphisms (GC-TRFLP). Examination of a soil microbial community through GC-TRFLP revealed 731 bacterial operational taxonomic units (OTUs) that followed a lognormal distribution. To recover the same 731 OTUs through analysis of DNA sequence data is estimated to require analysis of 86,264 16S rRNA sequences. The approach is examined and validated through construction and analysis of simulated microbial communities in silico. Additional simulations performed to assess the potential effects of PCR bias show that biased amplification can cause a community whose distribution follows a power-law function to appear lognormally distributed. We also show that TRFLP analysis, in contrast to GC-TRFLP, is not able to effectively distinguish between competing SAD models. Our analysis supports use of the lognormal as the null distribution for studying the SAD of bacterial communities as for plant and animal communities
Robust estimation of microbial diversity in theory and in practice
Quantifying diversity is of central importance for the study of structure,
function and evolution of microbial communities. The estimation of microbial
diversity has received renewed attention with the advent of large-scale
metagenomic studies. Here, we consider what the diversity observed in a sample
tells us about the diversity of the community being sampled. First, we argue
that one cannot reliably estimate the absolute and relative number of microbial
species present in a community without making unsupported assumptions about
species abundance distributions. The reason for this is that sample data do not
contain information about the number of rare species in the tail of species
abundance distributions. We illustrate the difficulty in comparing species
richness estimates by applying Chao's estimator of species richness to a set of
in silico communities: they are ranked incorrectly in the presence of large
numbers of rare species. Next, we extend our analysis to a general family of
diversity metrics ("Hill diversities"), and construct lower and upper estimates
of diversity values consistent with the sample data. The theory generalizes
Chao's estimator, which we retrieve as the lower estimate of species richness.
We show that Shannon and Simpson diversity can be robustly estimated for the in
silico communities. We analyze nine metagenomic data sets from a wide range of
environments, and show that our findings are relevant for empirically-sampled
communities. Hence, we recommend the use of Shannon and Simpson diversity
rather than species richness in efforts to quantify and compare microbial
diversity.Comment: To be published in The ISME Journal. Main text: 16 pages, 5 figures.
Supplement: 16 pages, 4 figure
Defending Our Public Biological Databases as a Global Critical Infrastructure
Progress in modern biology is being driven, in part, by the large amounts of freely available data in public resources such as the International Nucleotide Sequence Database Collaboration (INSDC), the world's primary database of biological sequence (and related) information. INSDC and similar databases have dramatically increased the pace of fundamental biological discovery and enabled a host of innovative therapeutic, diagnostic, and forensic applications. However, as high-value, openly shared resources with a high degree of assumed trust, these repositories share compelling similarities to the early days of the Internet. Consequently, as public biological databases continue to increase in size and importance, we expect that they will face the same threats as undefended cyberspace. There is a unique opportunity, before a significant breach and loss of trust occurs, to ensure they evolve with quality and security as a design philosophy rather than costly âretrofittedâ mitigations. This Perspective surveys some potential quality assurance and security weaknesses in existing open genomic and proteomic repositories, describes methods to mitigate the likelihood of both intentional and unintentional errors, and offers recommendations for risk mitigation based on lessons learned from cybersecurity
Knowledge Acquisition in Information System Development: A Case Study of System Developers in an International Bank
© 2016 John Wiley & Sons, Ltd. System developers quickly acquire knowledge of the benefits and limitations of system development approaches and this knowledge enables them to adopt or adapt methodologies according to the scope and scale of projects
The Solution Chemistry of Cu2+âtren Complexes Revisited: Exploring the Role of Species That Are Not Trigonal Bipyramidal
Potentiometric and spectrophotometric titrations indicate that aqueous solutions that contain equimolar amounts of
Cu2+ and tren contain the HCuL3+, CuL2+ and CuL(OH)+ species and that their relative concentrations depend on the pH of the solution. The stability constants and the UV/Vis and EPR spectra of the three species have been determined. The position of the absorption maximum clearly corresponds to a trigonal bipyramidal (tbp) geometry for CuL2+, whereas for HCuL3+ and CuL(OH)+ there are also bands that could correspond to square pyramidal (sp) complexes, but the EPR spectra indicate that only HCuL3+ can be considered to be sp. When any of these species is mixed with an excess of acid, an intermediate is formed within the mixing time of the stopped-flow technique. This intermediate undergoes complete decomposition in a second slower step. Interestingly, the spectrum of this intermediate is typical of sp geometry. Kinetic studies on complex formation in general indicate that complexation occurs in a single step, although under certain conditions an additional step has been observed that probably corresponds to the conversion of CuL2+ to HCuL3+, and the spectral changes indicate that the process involves structural reorganization from tbp to sp geometry. DFT and TDDFT calculations have been carried out for the three stable species, as well as for species in a higher protonation state. The results indicate that CuL2+ exists as a species with tetradentate tren and tbp geometry, although a wide range of distortions between the ideal tbp geometry and a geometry closer to sp is possible with a very modest energy cost. The energy change associated with hydrolysis of one of the Cuâ N bonds to give a species with tridentate tren was found to be slightly higher than that previously found for a related ligand, which contains a substituent at one of the terminal amino groups. For CuL(OH)+, the calculations suggest that an equilibrium exists between species with essentially the same energy but different geometries, each one of the species is closer to one of the ideal tbp and sp limits. For HCuL3+, the relevance of the sp geometry was confirmed by the calculations
Amplification by PCR Artificially Reduces the Proportion of the Rare Biosphere in Microbial Communities
The microbial world has been shown to hold an unimaginable diversity. The use of rRNA genes and PCR amplification to assess microbial community structure and diversity present biases that need to be analyzed in order to understand the risks involved in those estimates. Herein, we show that PCR amplification of specific sequence targets within a community depends on the fractions that those sequences represent to the total DNA template. Using quantitative, real-time, multiplex PCR and specific Taqman probes, the amplification of 16S rRNA genes from four bacterial species within a laboratory community were monitored. Results indicate that the relative amplification efficiency for each bacterial species is a nonlinear function of the fraction that each of those taxa represent within a community or multispecies DNA template. Consequently, the low-proportion taxa in a community are under-represented during PCR-based surveys and a large number of sequences might need to be processed to detect some of the bacterial taxa within the ârare biosphereâ. The structure of microbial communities from PCR-based surveys is clearly biased against low abundant taxa which are required to decipher the complete extent of microbial diversity in nature
The phylogenetic composition and structure of soil microbial communities shifts in response to elevated carbon dioxide
http://www.nature.com/ismej/journal/v6/n2/full/ismej201199a.htmlOne of the major factors associated with global change is the ever-increasing concentration of atmospheric CO2. Although the stimulating effects of elevated CO2 (eCO2) on plant growth and primary productivity have been established, its impacts on the diversity and function of soil microbial communities are poorly understood. In this study, phylogenetic microarrays (PhyloChip) were used to comprehensively survey the richness, composition and structure of soil microbial communities in a grassland experiment subjected to two CO2 conditions (ambient, 368 p.p.m., versus elevated, 560 p.p.m.) for 10 years. The richness based on the detected number of operational taxonomic units (OTUs) significantly decreased under eCO2. PhyloChip detected 2269 OTUs derived from 45 phyla (including two from Archaea), 55 classes, 99 orders, 164 families and 190 subfamilies. Also, the signal intensity of five phyla (Crenarchaeota, Chloroflexi, OP10, OP9/JS1, Verrucomicrobia) significantly decreased at eCO2, and such significant effects of eCO2 on microbial composition were also observed at the class or lower taxonomic levels for most abundant phyla, such as Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes and Acidobacteria, suggesting a shift in microbial community composition at eCO2. Additionally, statistical analyses showed that the overall taxonomic structure of soil microbial communities was altered at eCO2. Mantel tests indicated that such changes in species richness, composition and structure of soil microbial communities were closely correlated with soil and plant properties. This study provides insights into our understanding of shifts in the richness, composition and structure of soil microbial communities under eCO2 and environmental factors shaping the microbial community structure
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