Human chorionic gonadotropin and GnRH effects on pregnancy survival in pregnant cows and resynchronized pregnancy rates

Dairy Research, 2007 is known as Dairy Day, 2007Experiments have shown human chorionic gonadotropin (hCG) to be more effective than gondadotropin releasing hormone (GnRH) as a means to ovulate follicles. Experiment 1 determined the effects of replacing the first injection of GnRH (day 7) with hCG or saline in a Resynch-Ovsynch protocol on pregnancy rates in cows subsequently diagnosed not pregnant and pregnancy survival in cows subsequently diagnosed pregnant (day 0). A second study determined the ovulation potential of hCG compared with GnRH and saline (Exp. 2). In Exp. 1, cows in 4 herds were assigned randomly based on lactation number, number of previous AI, and last test-day milk yield to treatments of 1,000 IU of hCG, 100 μg of GnRH, or left as untreated controls 7 days before pregnancy diagnosis. Cows found not pregnant were given PGF2α (day 0), then inseminated 72 hours later, concurrent with a GnRH injection (3 herds) or given GnRH 16 to 24 hours before AI at 72 hours (1 herd). Timed AI pregnancy rates tended (P = 0.08) to be reduced by saline (12.9%; n = 505) compared with GnRH (17.9%; n = 703) but not hCG (16.5%; n = 541). Among pregnant cows treated, pregnancy survival 4 to 9 weeks after initial pregnancy diagnosis differed among herds (P < 0.001); but in 1 herd, GnRH reduced pregnancy survival, whereas hCG seemed to increase survival compared with control. Only small differences were detected in the other 3 herds, except for a slight negative effect of hCG compared with control in 1 herd. Ovarian structures were monitored in herd 1 by using transrectal ultrasonography 0 and 7 days after treatment with hCG, GnRH, or saline (Exp. 2). A tendency for a treatment × pregnancy status interaction (P = 0.07) was detected. Incidences of ovulation in nonpregnant cows were: hCG (51.6%; n = 126), GnRH (46.1%; n = 102), and control (28.1%; n = 96), whereas those in pregnant cows were: hCG (59.3%; n = 59), GnRH (24.5%; n = 49), and saline (6.9%; n = 58). We concluded that: 1) initiating a Resynch-Ovsynch protocol 7 days before pregnancy diagnosis with saline reduced timed AI pregnancy rates (Exp. 1); 2) in pregnant cows treated with GnRH, pregnancy survival was slightly reduced in 1 of 4 herds (Exp. 1); and 3) incidence of new corpus luteum (CL) was greater after hCG than GnRH in pregnant cows but not in nonpregnant cows (Exp. 2)

Evaluation of human chorionic gonadotropin as a replacement for GnRH in an ovulation synchronization protocol before fixed-time insemination

Two experiments were conducted to evaluate the difference between gonadotropinreleasing hormone (GnRH) and human chorionic gonadotropin (hCG) given at the beginning of a timed AI protocol and their effects on fertility. In Experiment 1, beef cows (n = 672) at six different locations were assigned randomly to treatments based on age, body condition, and days postpartum. On day −10, cattle were treated with GnRH or hCG and a progesterone-releasing controlled internal drug release (CIDR) insert was placed in the vagina. An injection of PGF2α was given and CIDR inserts were removed on day −3. Cows were inseminated at one fixed timed at 62 hr (day 0) after CIDR insert removal. Pregnancy was diagnosed at 33 days (range of 32 to 35) after insemination to determine pregnancy rates. For cows that were pregnant after the first insemination, a second pregnancy diagnosis was conducted 35 days (range of 33 to 37) after the first diagnosis to determine pregnancy survival. Pregnancy rates were reduced by the hCG injection compared with the GnRH injection (39.1 vs. 53.5%). In Experiment 2, cattle were assigned randomly to three treatments, balanced evenly across the two treatments (GnRH vs. hCG) applied in Experiment 1. Cows were injected with GnRH, hCG, or saline seven days before the first pregnancy diagnosis of cows inseminated in Experiment 1. At the time of pregnancy diagnosis, cattle found not pregnant (n = 328) were given PGF2α and inseminated 56 hours later. A second pregnancy diagnosis was conducted 35 days (range of 33 to 37) after the second insemination to determine pregnancy rate at the second AI. Injections of GnRH, hCG, or saline had no effect on pregnancy rates of cows already pregnant to the first insemination. Pregnancy rates after second insemination in cows given an injection of hCG or GnRH, however, tended to be reduced. Percentage of cows pregnant after two timed inseminations exceeded 60% without any need to detect estrus

The Footprint of F-theory at the LHC

Recent work has shown that compactifications of F-theory provide a potentially attractive phenomenological scenario. The low energy characteristics of F-theory GUTs consist of a deformation away from a minimal gauge mediation scenario with a high messenger scale. The soft scalar masses of the theory are all shifted by a stringy effect which survives to low energies. This effect can range from 0 GeV up to ~ 500 GeV. In this paper we study potential collider signatures of F-theory GUTs, focussing in particular on ways to distinguish this class of models from other theories with an MSSM spectrum. To accomplish this, we have adapted the general footprint method developed recently for distinguishing broad classes of string vacua to the specific case of F-theory GUTs. We show that with only 5 fb^(-1) of simulated LHC data, it is possible to distinguish many mSUGRA models and low messenger scale gauge mediation models from F-theory GUTs. Moreover, we find that at 5 fb^(-1), the stringy deformation away from minimal gauge mediation produces observable consequences which can also be detected to a level of order ~ +/- 80 GeV. In this way, it is possible to distinguish between models with a large and small stringy deformation. At 50 fb^(-1), this improves to ~ +/- 10 GeV.Comment: 85 pages, 37 figure

Sapling size influences shade tolerance ranking among southern boreal tree species

1 Traditional rankings of shade tolerance of trees make little reference to individual size. However, greater respiratory loads with increasing sapling size imply that larger individuals will be less able to tolerate shade than smaller individuals of the same species and that there may be shifts among species in shade tolerance with size. 2 We tested this hypothesis using maximum likelihood estimation to develop individual-tree-based models of the probability of mortality as a function of recent growth rate for seven species: trembling aspen, paper birch, yellow birch, mountain maple, white spruce, balsam fir and eastern white cedar. 3 Shade tolerance of small individuals, as quantified by risk of mortality at low growth, was mostly consistent with traditional shade tolerance rankings such that cedar > balsam fir > white spruce > yellow birch > mountain maple = paper birch > aspen. 4 Differences in growth-dependent mortality were greatest between species in the smallest size classes. With increasing size, a reduced tolerance to shade was observed for all species except trembling aspen and thus species tended to converge in shade tolerance with size. At a given level of radial growth larger trees, apart from aspen, had a higher probability of mortality than smaller trees. 5 Successional processes associated with shade tolerance may thus be most important in the seedling stage and decrease with ontogeny

Comprehensive analysis of epigenetic clocks reveals associations between disproportionate biological ageing and hippocampal volume

The concept of age acceleration, the difference between biological age and chronological age, is of growing interest, particularly with respect to age-related disorders, such as Alzheimer’s Disease (AD). Whilst studies have reported associations with AD risk and related phenotypes, there remains a lack of consensus on these associations. Here we aimed to comprehensively investigate the relationship between five recognised measures of age acceleration, based on DNA methylation patterns (DNAm age), and cross-sectional and longitudinal cognition and AD-related neuroimaging phenotypes (volumetric MRI and Amyloid-β PET) in the Australian Imaging, Biomarkers and Lifestyle (AIBL) and the Alzheimer’s Disease Neuroimaging Initiative (ADNI). Significant associations were observed between age acceleration using the Hannum epigenetic clock and cross-sectional hippocampal volume in AIBL and replicated in ADNI. In AIBL, several other findings were observed cross-sectionally, including a significant association between hippocampal volume and the Hannum and Phenoage epigenetic clocks. Further, significant associations were also observed between hippocampal volume and the Zhang and Phenoage epigenetic clocks within Amyloid-β positive individuals. However, these were not validated within the ADNI cohort. No associations between age acceleration and other Alzheimer’s disease-related phenotypes, including measures of cognition or brain Amyloid-β burden, were observed, and there was no association with longitudinal change in any phenotype. This study presents a link between age acceleration, as determined using DNA methylation, and hippocampal volume that was statistically significant across two highly characterised cohorts. The results presented in this study contribute to a growing literature that supports the role of epigenetic modifications in ageing and AD-related phenotypes

Measurements of differential production cross sections for a Z boson in association with jets in pp collisions at root s=8 TeV

Peer reviewe

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data