Critical User Journey Test Coverage

In the space of software testing, making sure that all critical user scenarios or journeys (CUJs) in a product have been tested is important. A CUJ is essentially a series of steps in the product taken by users of the product to achieve a desired outcome. Ensuring CUJ coverage during testing is a difficult problem that often requires subject matter experts to identify critical scenarios and expensive manual efforts to track coverage. This disclosure describes automatic extraction of CUJs based on analysis of test logs and production logs, e.g., traffic logs at a website. Data mining techniques are applied to merge various sources of data, deduce critical user journeys, and prioritize them during testing. Code release can be gated based on test coverage of a threshold proportion of the identified CUJ

Lipids Trigger a Conformational Switch That Regulates Signal Recognition Particle (SRP)-mediated Protein Targeting*

Co-translational protein targeting to the membrane is mediated by the signal recognition particle and its receptor (FtsY). Their homologous GTPase domains interact at the membrane and form a heterodimer in which both GTPases are activated. The prerequisite for protein targeting is the interaction of FtsY with phospholipids. However, the mechanism of FtsY regulation by phospholipids remained unclear. Here we show that the N terminus of FtsY (A domain) is natively unfolded in solution and define the complete membrane-targeting sequence. We show that the membrane-targeting sequence is highly dynamic in solution, independent of nucleotides and directly responds to the density of anionic phospholipids by a random coil-helix transition. This conformational switch is essential for tethering FtsY to membranes and activates the GTPase for its subsequent interaction with the signal recognition particle. Our results underline the dynamics of lipid-protein interactions and their importance in the regulation of protein targeting and translocation across biological membranes