Spasmolytic Effects of Aqueous Extract of Mixture from Aframomumum Melegueta (K Schum) – Citrus Aurantifolia (Christm and Panzer) on Isolated Trachea from Rat

The spasmolytic properties of the aqueous extract of Aframomum melegueta (K Schum) and Citrus aurantifolia (Christm and Panzer) (AMCA) mixture were tested on isolated rat trachea. Inhibition of the contraction was observed the in presence of the AMCA (EC 50 = 1.80 ± 0.48 mg/mL) after a pre contraction of the trachea by acetylcholine (10-5 M). With propranolol (10-6 M), the spasmolytic activity of the mixture was inhibited and the concentration-response curve shifted to the right. The EC50 value was then 2.60 ± 0.41 mg/mL. AMCA also inhibited contraction induced by KCl (4.10-2 M) with EC50 value = 1.86 ± 0.65 mg/mL. These results clearly show the relaxing effect of the aqueous extract on the isolated rat trachea. This effect involved some β-adrenergic receptor inhibition.Key words: Aframomum melegueta; Citrus aurantifolia; extract; bronchodilatory; spasmolytic, Rat trachea

Diversité des systèmes d’élevages de bovins de race bovine Borgou dans la zone soudanienne du Bénin

La caractérisation des systèmes d’élevage permet d’identifier la diversité des élevages afin d’élaborer des actions de développement. Le but de cette étude est de caractériser les types d’élevages de bovins de race Borgou dans la zone septentrionale du Bénin. La collecte des données a eu lieu dans 40 élevages dans l’Alibori, 24 dans l’Atacora et 84 dans le Borgou, de juin à décembre 2010. La procédure Proc corresp a été utilisée pour l’analyse factorielle des correspondances (AFC). Les Peulh représentent l’ethnie majoritaire des éleveurs (62,5 à 95,8%). Les élevages de type 1 se rencontrent dans les Communes de Pèrèrè, de Banikoara et chez un groupe d’éleveurs de Tchaourou. Ils sont des éleveurs professionnels ou des agriculteurs et font le plus souvent un élevage de prestige. Ils sont le plus souvent des Peulh ou des Gando. Dans le type 2, les éleveurs de bovins de Kèrou et du second groupe d’éleveurs de Tchaourou sont des éleveurs professionnels et  secondairement des agriculteurs. Ils sont pour la plupart Peulh. Ils vendent du lait, des animaux de trait et des bovins de boucherie élevés en embouche herbagère sur pâturage naturel. Le type 3 concerne les éleveurs de bovins de Parakou qui ont la boucherie ou le commerce comme activité principale et qui produisent de la viande à travers l’engraissement des animaux. Ils sont Bariba ou Dendi. La caractérisation des trois types d’élevage permettra de proposer des actions de développement intégrées. © 2013 International Formulae Group. All rights reserved.Mots clés: Alimentation, économie, habitat, pathologie, système d’élevage

Relationships between carcass traits and offal components in local poultry populations (gallus gallus) of Benin

peer reviewedObjectives : The current work was carried out to determine the relationships between live weight, carcass traits and the offal components traits in Holli, Fulani, Sahoue, North and Southe indigenous chicken ectoypes of Benin

Phenotypic cha- racterization of pig genetic resources in the departments of Oueme and Plateau in Benin

L’élevage porcin est très pratiqué au Sud-Bénin et implique une diversité de races ou de populations. L’objectif de l’étude était de caractériser les différents porcs sur le plan morphométrique et phénotypique. Ainsi, les données phéno- typiques ont été collectées sur 149 porcs, dont 14 améliorés, 91 croisés et 44 locaux. Les porcs de type génétique local ont présenté des mesures morpho- logiques significativement inférieures (p < 0,05) à celles des porcs améliorés et des animaux issus des croisements entre les porcs améliorés et les porcs locaux. Les poils des porcs locaux étaient significativement plus courts (p < 0,05) que ceux des porcs améliorés et des croisés. La couleur de la robe a varié d’un type génétique à l’autre. La couleur la plus rencontrée a été le blanc uniforme, suivi du noir uniforme chez tous les types génétiques. Le profil de la tête était plus rectiligne chez les porcs locaux, et plus concave chez les porcs améliorés et chez les croisés. Les oreilles dressées étaient moins observées chez les croisés. Elles étaient orientées vers l’avant chez les porcs améliorés et chez les croisés alors qu’elles étaient dressées et orientées vers l’arrière chez les porcs locaux. La queue en tire-bouchon a été significativement (p < 0,05) plus présente chez les porcs améliorés que chez les porcs croisés, et elle a été plus présente chez ces derniers que chez les porcs locaux. La ligne dorsale droite a été davantage observée chez les porcs locaux et chez les croisés que chez les porcs amélio- rés chez lesquels la ligne était plus creuse. Les porcs croisés ont présenté une grande similarité avec les porcs améliorés

Phenotypic characterisation and molecular polymorphism of indigenous poultry populations of the species Gallus gallus of Savannah and Forest ecotypes of Benin

The study of the phenotypic characterisation and molecular polymorphism of local chicken populations was carried out in Benin on 326 chickens of the Forest ecological area and 316 of the Savannah ecological area, all were 7 months old at least. The collection of blood for the molecular typing wasachieved on 121 indigenous chickens of which 60 from the Savannah ecological area and 61 from the Forest ecological area. The genotyping was carried out for 22 microsatellite loci. Weight and body measures of the Savannah chickens were significantly higher (P &lt; 0.001) than those of the Forest chickens. In the Savannah ecological area, the most frequent plumage colours were the black (22.15%), the white (19.62%), the coppery black (7.59%) and the golden partridge (7.59%). In the Forest area, thefawn (15.34%), the black (10.43%), the white (6.8%), the silver white (6.8%) and the golden partridge (6.75%) were the dominant feather colours. Thus, phenotypic characterisation showed significant differences between Savannah and Forest local chickens. The FST calculated between the Savannah and Forest populations revealed a low genetic differentiation and the dendogram showed that Savannah and Forest chickens were quite intermingled. In conclusion, local populations from Savannah andForest area may be considered as ecotypes, but not as two distinct breeds

Comparison of growth performance, carcass characteristics and meat quality of Benin indigenous chickens and Label Rouge (T55×SA51)

A study on growth performance, carcass traits and meat quality was carried out on Savannah and Forest  ecotype chicken of Benin, using Label Rouge (T55 X SA51) as a control genotype. All the animals were fed ad  libitum with three diets (starter, grower and layer feed). They were individually weighed at hatching and during the growing stage, and the daily feed intake was recorded. A sample of 12 males of each genetic type was  slaughtered for carcass characteristics. Sensory analysis was done on each genetic type after boiling or  roasting. The Label Rouge chickens were heavier than the local chickens at hatch (P&lt;0.001). At the end of 40  weeks, the weight of the Label Rouge was double that of the Savannah ecotype and 2.8 times that of the Forest ecotype. The feed efficiency of the Label Rouge was higher than that of the local chickens (P&lt;0.001). The  genetic type influenced tenderness and juiciness, with the local Savannah chicken being the most tender and  juiciest (P&lt;0.001). However, the cooking method and the carcass cut influenced tenderness only. The overall assessment of the meat of the Label Rouge chickens was similar to that of the local chickens, whereas the  assessment of the meat was significantly lower for local chickens of Forest ecotype compared to the Savannah ecotype (P&lt;0.001). The local chickens would therefore be suitable for improving traditional poultry production, whereas controlled crossbreeding programmes using Label Rouge could be recommended to improve local chicken weight.Key words: Growth, carcass, sensory characters, indigenous chicken, Label Rouge

Use of chips and cassava leaves in finishing guinea (Numida meleagris, L): animal performance, costs of production, Aspect of the carcass and meat quality

peer reviewedThe effect of feed containing cassava leafs and peels was assessed on 126 local guinea fowls animal performance, production costs and meat quality over a period of 28 weeks in humid tropical climate of the South Benin. After 12 weeks of classical feeding, animals were randomly allotted in three groups of 42 for the finishing period: the group 1 received a control diet while, in group 2, 8% and 35 % of cassava leafs and cossets were respectively incorporated, vs. 6 % and 25 % in group 3. At the end of the trial, eighteen guinea fowls per group were slaughtered in order to determine characteristics of the carcass and meat quality. Individual daily feed intake was higher in the control group than in the groups 2 and 3. Animals from control group shown higher growth rate (5.0 g/d) (P<0.05) than in groups 2 and 3. Consequently, feed conversion ratios were similar in the three groups with values of 7.5, 6.7 and 6.9, in groups 1, 2 and 3, respectively. At the end of the trial, animals in control group were heavier than those in groups 2 and 3 (P<0.05). No negative influence of cassava cossets and leaf meals was observed on carcass quality in the experimental diets. Feed costs per kg live weight were reduced by 24.6 and 21.0% in groups 2 and 3, respectively, when compared to control group. Guinea fowl production appears thus more profitable with feed containing cassava leaves and cossets. Consequently, these ingredients could be alternative sources of energy and protein, with emphasis during the finishing period

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Global economic burden of unmet surgical need for appendicitis

Background: There is a substantial gap in provision of adequate surgical care in many low-and middle-income countries. This study aimed to identify the economic burden of unmet surgical need for the common condition of appendicitis. Methods: Data on the incidence of appendicitis from 170 countries and two different approaches were used to estimate numbers of patients who do not receive surgery: as a fixed proportion of the total unmet surgical need per country (approach 1); and based on country income status (approach 2). Indirect costs with current levels of access and local quality, and those if quality were at the standards of high-income countries, were estimated. A human capital approach was applied, focusing on the economic burden resulting from premature death and absenteeism. Results: Excess mortality was 4185 per 100 000 cases of appendicitis using approach 1 and 3448 per 100 000 using approach 2. The economic burden of continuing current levels of access and local quality was US $92 492 million using approach 1 and$73 141 million using approach 2. The economic burden of not providing surgical care to the standards of high-income countries was $95 004 million using approach 1 and$75 666 million using approach 2. The largest share of these costs resulted from premature death (97.7 per cent) and lack of access (97.0 per cent) in contrast to lack of quality. Conclusion: For a comparatively non-complex emergency condition such as appendicitis, increasing access to care should be prioritized. Although improving quality of care should not be neglected, increasing provision of care at current standards could reduce societal costs substantially

Pooled analysis of WHO Surgical Safety Checklist use and mortality after emergency laparotomy

Background The World Health Organization (WHO) Surgical Safety Checklist has fostered safe practice for 10 years, yet its place in emergency surgery has not been assessed on a global scale. The aim of this study was to evaluate reported checklist use in emergency settings and examine the relationship with perioperative mortality in patients who had emergency laparotomy. Methods In two multinational cohort studies, adults undergoing emergency laparotomy were compared with those having elective gastrointestinal surgery. Relationships between reported checklist use and mortality were determined using multivariable logistic regression and bootstrapped simulation. Results Of 12 296 patients included from 76 countries, 4843 underwent emergency laparotomy. After adjusting for patient and disease factors, checklist use before emergency laparotomy was more common in countries with a high Human Development Index (HDI) (2455 of 2741, 89.6 per cent) compared with that in countries with a middle (753 of 1242, 60.6 per cent; odds ratio (OR) 0.17, 95 per cent c.i. 0.14 to 0.21, P <0001) or low (363 of 860, 422 per cent; OR 008, 007 to 010, P <0.001) HDI. Checklist use was less common in elective surgery than for emergency laparotomy in high-HDI countries (risk difference -94 (95 per cent c.i. -11.9 to -6.9) per cent; P <0001), but the relationship was reversed in low-HDI countries (+121 (+7.0 to +173) per cent; P <0001). In multivariable models, checklist use was associated with a lower 30-day perioperative mortality (OR 0.60, 0.50 to 073; P <0.001). The greatest absolute benefit was seen for emergency surgery in low- and middle-HDI countries. Conclusion Checklist use in emergency laparotomy was associated with a significantly lower perioperative mortality rate. Checklist use in low-HDI countries was half that in high-HDI countries.Peer reviewe