5 research outputs found

    Accelerated in vitro propagation of elite oil palm genotypes (Elaeis guineensis Jacq.) by substituting cytokinin with putrescine

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    Clonal multiplication of oil palm enables the formation of commercial plantations with higher yield, which is possible through somatic embryogenesis. However, due to different modifications made in the existing protocols to avoid the appearance of the “mantled flower”, the success of this technique has been limited. Thus, this study aimed to apply a cloning protocol, using somatic embryogenesis, to 32 elite oil palm genotypes from a commercial plantation on which cytokinin was substituted with putrescine. All tested genotypes responded positively to callus induction. Moreover, the percentages of responses were differentiated: 65.62% of these genotypes produced embryogenic lines, and 40.62% out of that percentage presented lines with moderate or high multiplication capacity, which is the main factor that enabled the obtaining of clones to form a commercial clonal plantation. The somatic embryogenesis was efficient, making this protocol applicable at a commercial scale, since it allows the obtaining of clones up to 360 days. However, future evaluations on these clones are needed to investigate the appearance of the “mantled flower”.Keywords: Cloning, polyamines, tissue culture, commercial plantatio

    Outcomes from elective colorectal cancer surgery during the SARS-CoV-2 pandemic

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    This study aimed to describe the change in surgical practice and the impact of SARS-CoV-2 on mortality after surgical resection of colorectal cancer during the initial phases of the SARS-CoV-2 pandemic

    Elective Cancer Surgery in COVID-19–Free Surgical Pathways During the SARS-CoV-2 Pandemic: An International, Multicenter, Comparative Cohort Study

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    Delaying surgery for patients with a previous SARS-CoV-2 infection

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    The impact of surgical delay on resectability of colorectal cancer: An international prospective cohort study

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    AimThe SARS-CoV-2 pandemic has provided a unique opportunity to explore the impact of surgical delays on cancer resectability. This study aimed to compare resectability for colorectal cancer patients undergoing delayed versus non-delayed surgery.MethodsThis was an international prospective cohort study of consecutive colorectal cancer patients with a decision for curative surgery (January-April 2020). Surgical delay was defined as an operation taking place more than 4 weeks after treatment decision, in a patient who did not receive neoadjuvant therapy. A subgroup analysis explored the effects of delay in elective patients only. The impact of longer delays was explored in a sensitivity analysis. The primary outcome was complete resection, defined as curative resection with an R0 margin.ResultsOverall, 5453 patients from 304 hospitals in 47 countries were included, of whom 6.6% (358/5453) did not receive their planned operation. Of the 4304 operated patients without neoadjuvant therapy, 40.5% (1744/4304) were delayed beyond 4 weeks. Delayed patients were more likely to be older, men, more comorbid, have higher body mass index and have rectal cancer and early stage disease. Delayed patients had higher unadjusted rates of complete resection (93.7% vs. 91.9%, P = 0.032) and lower rates of emergency surgery (4.5% vs. 22.5%, P ConclusionOne in 15 colorectal cancer patients did not receive their planned operation during the first wave of COVID-19. Surgical delay did not appear to compromise resectability, raising the hypothesis that any reduction in long-term survival attributable to delays is likely to be due to micro-metastatic disease
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