Experimental measurement of the Melnikov function

International audienceWe study the transport properties of a genuine two-dimensional flow with a large mean velocity perturbed periodically in time by means of an original experimental technique. The flow, generated by the co-rotation of two cylinders is both stratified with a linear density gradient using salted water, and viscous in order to prevent Ekman pumping and centrifugal instabilities. Thus, the mean flow contains a hy-perbolic point with a homoclinic streamline, which we perturb periodically by an extra oscillation. A blob of scalar injected close to the stagnation point contracts on the stable manifold, and stretches in the unstable direction. The distance between the stable and the unstable manifolds is measured as the distance between the maximum and the minimum of the dye undulating pattern, and is recorded as a function of the perturbation frequency. This distance, also called the Melnikov function, presents a maximum when the residence time of a fluid particle in the mean flow is about half a perturbation period. This resonance criterion is recovered with good quantitative agreement by the theoretical prediction of the Melnikov function computed for this flow

Emodin Prevents Intrahepatic Fat Accumulation, Inflammation and Redox Status Imbalance During Diet-Induced Hepatosteatosis in Rats

High-fat and/or high-carbohydrate diets may predispose to several metabolic disturbances including liver fatty infiltration (hepatosteatosis) or be associated with necro-inflammation and fibrosis (steatohepatitis). Several studies have emphasized the hepatoprotective effect of some natural agents. In this study, we investigated the potential therapeutic effects of the treatment with emodin, an anthraquinone derivative with anti-oxidant and anti-cancer abilities, in rats developing diet-induced hepatosteatosis and steatohepatitis. Sprague-Dawley rats were fed a standard diet (SD) for 15 weeks, or a high-fat/high-fructose diet (HFD/HF). After 5 weeks, emodin was added to the drinking water of some of the SD and HFD/HF rats. The experiment ended after an additional 10 weeks. Emodin-treated HFD/HF rats were protected from hepatosteatosis and metabolic derangements usually observed in HFD/HF animals. Furthermore, emodin exerted anti-inflammatory activity by inhibiting the HFD/HF-induced increase of tumor necrosis factor (TNF)-α. Emodin also affected the hepatocytes glutathione homeostasis and levels of the HFD/HF-induced increase of glutathionylated/phosphorylated phosphatase and tensin homolog (PTEN). In conclusion, we demonstrated that a natural agent such as emodin can prevent hepatosteatosis, preserving liver from pro-inflammatory and pro-oxidant damage caused by HFD/HF diet. These findings are promising, proposing emodin as a possible hindrance to progression of hepatosteatosis into steatohepatitis

Resonances and instabilities in a tilted rotating annulus

The flow inside a rotating annulus tilted with respect to the gravity is characterized experimentally and theoretically. As in the case of a tilted rotating cylinder the flow is forced by the free surface, maintained flat by gravity. It leads to resonances of global inertial modes (Kelvin modes) when the height of fluid is a multiple of half the wavelength of the mode. The divergence of the mode is saturated by viscous effects at the resonance. The maximum amplitude scales as the Ekman number to the power −1/2 when surface Ekman pumping is dominant and to the power −1 when volumic damping is dominant. An analytical prediction is given with no fitting parameter, in excellent agreement with experimental results. At lower Ekman numbers, the flow destabilises with respect to a triadic resonance instability, as already observed by Xu and Harlander, Phys. Rev. Fluids 2020. We provide here a linear stability analysis leading to the viscous threshold of the instability for small tilt angles. For large tilt angles, a centrifugal instability is observed due to the acceleration of the flow by the inner cylinder. Finally, the features of the turbulent flow and its mixing efficiency are characterized experimentally. We underline the potential interest of this configuration for bioreactors

Tilted rotating annular bioreactors

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Gold–phosphine–porphyrin as potential metal-based theranostics

Two new gold-phosphine-porphyrin derivatives were synthesized and fully characterized, and their photophysical properties investigated along a water-soluble analog. The cytotoxicity of the compounds was tested on cancer cells (HCT116 and SW480), and their cell uptake was followed by fluorescence microscopy in vitro (on SW480). The proof that the water-soluble gold-phosphine-porphyrin is a biologically active compound that can be tracked in vitro was clearly established, especially concerning the water-soluble analog. Some preliminary photodynamic therapy (PDT) experiments were also performed. They highlight a dramatic increase of the cytotoxicity when the cells were illuminated for 30 min with white light