Aspectos dos tempos verbais

In this paper I present two tenses of the German verbal system, the so called Doppelperfekt and Doppelplusquamperfekt. Although these tenses have only been marginally dealt with in the grammars, more studies have recently been made on them within the field of Linguistics. In order to describe these tenses, I will concentrate on the following authors: Hauser-Suida & Hoppe-Beugel (1972), Eroms (1984), Thieroff (1992) und Vater (1994). The tenses will be analysed formally and their meaning and usage illustrated with examples taken from the articles above.In diesem Aufsatz werde ich zwei Tempora der Vergangenheit im Deutschen behandeln. das Doppelperfekt und das Doppelplusquamperfekt. Ein Grund dafür, gerade diese beiden Formen zu beschreiben, liegt darin, daß sie einerseits in den Grammatiken kaum berücksichtigt werden, in entsprechenden linguistischen Arbeiten aber einen neuen Aufschwung bekommen haben. Für die Beschreibung beziehe ich mich überwiegend auf die Arbeiten von Hauser-Suida & Hoppe-Beugel (1992), Eroms (1984), Thieroff (1992) und Vater (1994). Die Formen werden zuerst rein formal und dann in ihrer Bedeutung und Anwendung beschrieben, ergänzt durch Beispiele, die den oben genannten Arbeiten entnommen wurden

O futuro existe?

From Vater's Thesis, I bring the discussion about the future verbal tense to the centre of discussion. The aim is to prove hat the future verbal tenses can also express time in German, and, therefore, they have to be included in the verbal System and, secondly, that the expression of modality can also be expressed by the future forms, and that it usually overlaps the notion of time.Ausgehend von Heinz Vaters These, das Futur der deutschen Verben habe keine temporale Bedeutung, stelle ich die Frage der Futurtempora erneut zur Diskussion. Meine Absicht ist es, zunächst zu beweisen, dass auch im Deutschen die Futurtempora Zeit ausdrücken und daher als Tempora in das Verbalsystem aufgenommen werden können, aber dass der modale Charakter bei den Futurtempora auch eine wichtige Rolle spielt

Aktionsart

This paper presents a discussion on aspect and 'Aktionsart' as categories of verbal forms in German and Portuguese. The distinction between the two categories is based on the assumption that 'Aktionsart' is more important in the German verb system, whereas aspect plays a more significant role in Portuguese. Aspect as a morpho-semantic category may be further specified by 'Akrionsart', which is a lexical semantic category and belongs to the meaning of the verb.In diesem Aufsatz werde ich eine Unterscheidung zwischen Aspekt und 'Aktionsart' vornehmen. Für die Unterscheidung gehe ich davon aus, daß die Aktionsart eine wichtigere Rolle im deutschen Verbalsystem spielt, während im portugiesischen Verbalsystem der Aspekt wichtiger ist. Aspekt ist eine morphosemantische Kategorie und kann durch die Aktionsart weiter bestimmt werden. Diese ihrerseits gehört als lexikal semantische Kategorie zur Bedeutung des Verbs

Os verbos "ser" e "estar" em oposição ao verbo "sein" do alemão

People who learn Portuguese usually have difficulties in using two of the most frequent verbs of the Portuguese verbal system: ser and estar. Native speakers of German for example fail to easily identify the differences between these verbs, which are compared with the German verb sein. Our purpose is to describe these verbs, their meaning and function, and also to attempt to find criteria to help learners to identify the differences to use these verbs. Some of the differences can be explained by the speakers experiences and the context.Lerner des Portugiesischen als Fremdsprache haben oft Schwierigkeiten, die zwei häufig gebrauchten Verben des portugiesischen Verbalsystems ser und estar zu verwenden. Deutschsprachige erkennen kaum die Unterschiede zwischen diesen Verben, die im Deutschen oft mit dem Verb sein gleichgesetzt werden, das auch in ähnlichen Strukturen und Situationen vorkommt. In dieser Arbeit beschäftigen wir uns damit, diese Verben in ihrer Funktion und Bedeutung zu beschreiben und versuchen, Kriterien aufzustellen, die dem Lernenden helfen können, diese Unterschiede besser zu verstehen und dadurch die Verben besser anzuwenden. Einige Unterschiede können durch die Erfahrungen des Sprechers und durch den Kontext erklärt werden

Dosimetric response of radiochromic films to protons of low energies in the Bragg peak region

One of the major advantages of proton or ion beams, applied in cancer treatment, is their excellent depth-dose profile exhibiting a low dose in the entrance channel and a distinct dose maximum (Bragg peak) near the end of range in tissue. In the region of the Bragg peak, where the protons or ions are almost stopped, experimental studies with low-energy particle beams and thin biological samples may contribute valuable information on the biological effectiveness in the stopping region. Such experiments, however, require beam optimization and special dosimetry techniques for determining the absolute dose and dose homogeneity for very thin biological samples. At the National Centre of Accelerators in Seville, one of the beam lines at the 3 MV Tandem Accelerator was equipped with a scattering device, a special parallel-plate ionization chamber with very thin electrode foils and target holders for cell cultures. In this work, we present the calibration in absolute dose of EBT3 films [Gafchromic radiotherapy films, http://www.ashland.com/products/gafchromic-radiotherapy-films] for proton energies in the region of the Bragg peak, where the linear energy transfer increases and becomes more significant for radiobiology studies, as well as the response of the EBT3 films for different proton energy values. To irradiate the films in the Bragg peak region, the energy of the beam was degraded passively, by interposing Mylar foils of variable thickness to place the Bragg peak inside the active layer of the film. The results obtained for the beam degraded in Mylar foils are compared with the dose calculated by means of the measurement of the beam fluence with an ionization chamber and the energy loss predicted by srim2008 code.EU Initial Training Marie Curie Network FP7-PEOPLE-2011-ITN-289485Spanish Research Projects No. FPA2014-53290-C2-2-PSpanish Research Projects No. FPA2013-47327-C2-1-RAndalusian Research Project No. P12-FQM-160

Rapamycin Combined with Anti-CD45RB mAb and IL-10 or with G-CSF Induces Tolerance in a Stringent Mouse Model of Islet Transplantation

Background: A large pool of preexisting alloreactive effector T cells can cause allogeneic graft rejection following transplantation. However, it is possible to induce transplant tolerance by altering the balance between effector and regulatory T (Treg) cells. Among the various Treg-cell types, Foxp3 +Treg and IL-10-producing T regulatory type 1 (Tr1) cells have frequently been associated with tolerance following transplantation in both mice and humans. Previously, we demonstrated that rapamycin+IL-10 promotes Tr1-cell-associated tolerance in Balb/c mice transplanted with C57BL/6 pancreatic islets. However, this same treatment was unsuccessful in C57BL/6 mice transplanted with Balb/c islets (classified as a stringent transplant model). We accordingly designed a protocol that would be effective in the latter transplant model by simultaneously depleting effector T cells and fostering production of Treg cells. We additionally developed and tested a clinically translatable protocol that used no depleting agent. Methodology/Principal Findings: Diabetic C57BL/6 mice were transplanted with Balb/c pancreatic islets. Recipient mice transiently treated with anti-CD45RB mAb+rapamycin+IL-10 developed antigen-specific tolerance. During treatment, Foxp3 +Treg cells were momentarily enriched in the blood, followed by accumulation in the graft and draining lymph node, whereas CD4 +IL-10 +IL-4 - T (i.e., Tr1) cells localized in the spleen. In long-term tolerant mice, only CD4 +IL-10 +IL-4 - T cells remained enriched in the spleen and IL-10 was key in the maintenance of tolerance. Alternatively, recipient mice were treated with two compounds routinely used in the clinic (namely, rapamycin and G-CSF); this drug combination promoted tolerance associated with CD4 +IL-10 +IL-4 - T cells. Conclusions/Significance: The anti-CD45RB mAb+rapamycin+IL-10 combined protocol promotes a state of tolerance that is IL-10 dependent. Moreover, the combination of rapamycin+G-CSF induces tolerance and such treatment could be readily translatable into the clinic. © 2011 Gagliani et al

Sinonasal mucosal melanoma: Molecular profile and therapeutic implications from a series of 32 cases

BACKGROUND: Primary sinonasal mucosal melanomas are aggressive tumors with a poor clinical control by current treatments, raising the urgent need of novel strategies. METHODS: By fluorescence in situ hybridization (FISH), direct sequencing, and immunohistochemistry, we investigate the spectrum of molecular abnormalities in a cohort of 32 cases of primary sinonasal mucosal melanomas. RESULTS: We found that all primary sinonasal mucosal melanomas lack BRAF V600E mutation; in addition, they are characterized by somatic mutations of NRAS (22%) and KIT (12.5%), together with amplification of RREB1 (100%) and loss of MYB (76%). The large majority of cases showed KIT protein expression (96.9%). Among tumor suppressor genes, primary sinonasal mucosal melanomas showed loss of PTEN (48.1%) and p16/INK4a (55.2%). All tested cases showed expression of pAkt and pErk, suggesting a combined activation of PI3K/Akt and RAS-mitogen-activated protein kinase (MAPK) pathways. CONCLUSIONS: This molecular fingerprint strongly argues against the clinical efficacy of BRAF-inhibitors, but could candidate primary sinonasal mucosal melanomas to therapeutic strategies targeting RAS and KIT mutations or inhibiting PI3K-Akt-mTOR pathway

The HIV Tat protein affects processing of ribosomal RNA precursor

<p>Abstract</p> <p>Background</p> <p>Inside the cell, the HIV Tat protein is mainly found in the nucleus and nucleolus. The nucleolus, the site of ribosome biogenesis, is a highly organized, non-membrane-bound sub-compartment where proteins with a high affinity for nucleolar components are found. While it is well known that Tat accumulates in the nucleolus via a specific nucleolar targeting sequence, its function in this compartment it still unknown.</p> <p>Results</p> <p>To clarify the significance of the Tat nucleolar localization, we induced the expression of the protein during oogenesis in <it>Drosophila melanogaster </it>strain transgenic for HIV-<it>tat </it>gene. Here we show that Tat localizes in the nucleoli of <it>Drosophila </it>oocyte nurse cells, where it specifically co-localizes with fibrillarin. Tat expression is accompanied by a significant decrease of cytoplasmic ribosomes, which is apparently related to an impairment of ribosomal rRNA precursor processing. Such an event is accounted for by the interaction of Tat with fibrillarin and U3 snoRNA, which are both required for pre-rRNA maturation.</p> <p>Conclusion</p> <p>Our data contribute to understanding the function of Tat in the nucleolus, where ribosomal RNA synthesis and cell cycle control take place. The impairment of nucleolar pre-rRNA maturation through the interaction of Tat with fibrillarin-U3snoRNA complex suggests a process by which the virus modulates host response, thus contributing to apoptosis and protein shut-off in HIV-uninfected cells.</p

Measurement of the cross-section and charge asymmetry of WW bosons produced in proton-proton collisions at s=8\sqrt{s}=8 TeV with the ATLAS detector

This paper presents measurements of the $W^+ \rightarrow \mu^+\nu$ and $W^- \rightarrow \mu^-\nu$ cross-sections and the associated charge asymmetry as a function of the absolute pseudorapidity of the decay muon. The data were collected in proton--proton collisions at a centre-of-mass energy of 8 TeV with the ATLAS experiment at the LHC and correspond to a total integrated luminosity of 20.2~\mbox{fb^{-1}}. The precision of the cross-section measurements varies between 0.8% to 1.5% as a function of the pseudorapidity, excluding the 1.9% uncertainty on the integrated luminosity. The charge asymmetry is measured with an uncertainty between 0.002 and 0.003. The results are compared with predictions based on next-to-next-to-leading-order calculations with various parton distribution functions and have the sensitivity to discriminate between them.Comment: 38 pages in total, author list starting page 22, 5 figures, 4 tables, submitted to EPJC. All figures including auxiliary figures are available at https://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/STDM-2017-13

Search for chargino-neutralino production with mass splittings near the electroweak scale in three-lepton final states in √s=13 TeV pp collisions with the ATLAS detector

A search for supersymmetry through the pair production of electroweakinos with mass splittings near the electroweak scale and decaying via on-shell W and Z bosons is presented for a three-lepton final state. The analyzed proton-proton collision data taken at a center-of-mass energy of √s=13  TeV were collected between 2015 and 2018 by the ATLAS experiment at the Large Hadron Collider, corresponding to an integrated luminosity of 139  fb−1. A search, emulating the recursive jigsaw reconstruction technique with easily reproducible laboratory-frame variables, is performed. The two excesses observed in the 2015–2016 data recursive jigsaw analysis in the low-mass three-lepton phase space are reproduced. Results with the full data set are in agreement with the Standard Model expectations. They are interpreted to set exclusion limits at the 95% confidence level on simplified models of chargino-neutralino pair production for masses up to 345 GeV