Retrotransposons are specified as DNA replication origins in the gene-poor regions of Arabidopsis heterochromatin

Genomic stability depends on faithful genome replication. This is achieved by the concerted activity of thousands of DNA replication origins (ORIs) scattered throughout the genome. The DNA and chromatin features determining ORI specification are not presently known. We have generated a high-resolution genome-wide map of 3230 ORIs in cultured Arabidopsis thaliana cells. Here, we focused on defining the features associated with ORIs in heterochromatin. In pericentromeric gene-poor domains ORIs associate almost exclusively with the retrotransposon class of transposable elements (TEs), in particular of the Gypsy family. ORI activity in retrotransposons occurs independently of TE expression and while maintaining high levels of H3K9me2 and H3K27me1, typical marks of repressed heterochromatin. ORI-TEs largely colocalize with chromatin signatures defining GC-rich heterochromatin. Importantly, TEs with active ORIs contain a local GC content higher than the TEs lacking them. Our results lead us to conclude that ORI colocalization with retrotransposons is determined by their transposition mechanism based on transcription, and a specific chromatin landscape. Our detailed analysis of ORIs responsible for heterochromatin replication has implications on the mechanisms of ORI specification in other multicellular organisms in which retrotransposons are major components of heterochromatin and of the entire genome

Neuroepidemiologic study in Colombia based on administrative data (ESENCIA). Preliminary results 2015-2017

Introducción: con la transición demográfica y epidemiológica, se espera que la prevalencia de las patologías neurológicas aumente. En particular, Colombia no dispone de indicadores actualizados sobre la frecuencia de dichas enfermedades. Objetivo: determinar la prevalencia de 10 patologías neurológicas de relevancia (migraña, epilepsia, enfermedad de Alzheimer, ataque cerebrovascular isquémico y hemorrágico, enfermedad de Parkinson, esclerosis múltiple, miastenia gravis, meningitis, síndrome de Guillain-Barré y enfermedad de motoneurona) en pacientes con edad ≥15 años entre 2015-2017. Materiales y métodos: estudio de corte transversal a partir de fuentes administrativas, con base en los Registro Individuales de Prestación de Servicios (RIPS), consolidados por el Ministerio de Salud. Se analizaron las características sociodemográficas disponibles (sexo, grupo etario, régimen de salud, procedencia, etc.) y se ajustaron las prevalencias obtenidas según las distribuciones de la población mundial y nacional. Resultados: para el 2017, las prevalencias ajustadas a la distribución etaria mundial, por 100 000 habitantes, fueron: migraña 2170 (IC95 % 2164-2176); epilepsia 586 (IC95 % 583-589); enfermedad de Alzheimer 387 (IC95 % 384-389); ataque cerebrovascular 263 (IC95 % 260-265), específicamente, isquémico 136 (IC95 % 134-137) y hemorrágico 96 (IC95 % 95-98); enfermedad de Parkinson 91 (IC95 % 90-93), esclerosis múltiple 19 (IC95 % 18-19); miastenia gravis 11 (IC95% 11-11); meningitis 10 (IC95 % 9-10); síndrome de Guillain-Barré 9 (IC95 % 8-9); y enfermedad de motoneurona 6 (IC95 % 5-6). La mediana de variación 2015-2017 fue del 19,38%. Conclusión: Las patologías más prevalentes a nivel nacional, en orden de frecuencia, fueron migraña, epilepsia y enfermedad de Alzheimer.Introduction: With the demographic and epidemiological transition, the prevalence of neurological pathologies is expected to increase. In particular, Colombia does not have updated indicators regarding the frequency of these diseases. Objective: To determine the prevalence of ten relevant neurological pathologies (migraine, epilepsy, Alzheimer›s disease, ischemic and hemorrhagic stroke, Parkinson›s disease, multiple sclerosis, myasthenia gravis, meningitis, Guillain-Barré syndrome and motor neuron disease) in patients aged ≥15 years between 2015-2017. Materials and methods: Cross-sectional study of administrative sources, based on the Individual Service Provision Registries (RIPS) consolidated by the Ministry of Health. The available socio- demographic characteristics (sex, age group, health regime, origin, etc.) were analyzed, and the obtained prevalences were adjusted according to the distributions of the world and national population. Results: For 2017, the prevalences adjusted to the world age distribution, per 100,000 inhabitants, were: migraine 2170 (95%CI 2164-2176); epilepsy 586 (95%CI 583-589); Alzheimer›s disease 387 (95% CI 384-389); stroke 263 (95%CI 260-265), specifically, ischemic 136 (95%CI 134-137) and hemorrhagic (95% CI 95-98); Parkinson’s disease 91 (95%CI 90-93), multiple sclerosis 19 (95%CI 18-19); myasthenia gravis 11 (95%CI 11-11); meningitis 10 (95%CI 9-10); Guillain-Barré syndrome 9 (95%CI 8-9); and motor neuron disease 6 (95%CI 5-6). The 2015-2017 median variation was 19.38%. Conclusion: The most prevalent pathologies nationwide, in order of frequency, were migraine, epilepsy, and Alzheimer›s disease.Introducción: con la transición demográfica y epidemiológica, se espera que la prevalencia de las patologías neurológicas aumente. En particular, Colombia no dispone de indicadores actualizados sobre la frecuencia de dichas enfermedades. Objetivo: determinar la prevalencia de 10 patologías neurológicas de relevancia (migraña, epilepsia, enfermedad de Alzheimer, ataque cerebrovascular isquémico y hemorrágico, enfermedad de Parkinson, esclerosis múltiple, miastenia gravis, meningitis, síndrome de Guillain-Barré y enfermedad de motoneurona) en pacientes con edad ≥15 años entre 2015-2017. Materiales y métodos: estudio de corte transversal a partir de fuentes administrativas, con base en los Registro Individuales de Prestación de Servicios (RIPS), consolidados por el Ministerio de Salud. Se analizaron las características sociodemográficas disponibles (sexo, grupo etario, régimen de salud, procedencia, etc.) y se ajustaron las prevalencias obtenidas según las distribuciones de la población mundial y nacional. Resultados: para el 2017, las prevalencias ajustadas a la distribución etaria mundial, por 100 000 habitantes, fueron: migraña 2170 (IC95 % 2164-2176); epilepsia 586 (IC95 % 583-589); enfermedad de Alzheimer 387 (IC95 % 384-389); ataque cerebrovascular 263 (IC95 % 260-265), específicamente, isquémico 136 (IC95 % 134-137) y hemorrágico 96 (IC95 % 95-98); enfermedad de Parkinson 91 (IC95 % 90-93), esclerosis múltiple 19 (IC95 % 18-19); miastenia gravis 11 (IC95% 11-11); meningitis 10 (IC95 % 9-10); síndrome de Guillain-Barré 9 (IC95 % 8-9); y enfermedad de motoneurona 6 (IC95 % 5-6). La mediana de variación 2015-2017 fue del 19,38%. Conclusión: Las patologías más prevalentes a nivel nacional, en orden de frecuencia, fueron migraña, epilepsia y enfermedad de Alzheimer

COMUNICACIÓN GESTUAL Y GESTOS DE AUTOCONTACTO EN LOS COMERCIANTES DEL CENTRO COMERCIAL “SIETE COLINAS” DE GUARANDA

El trabajo de investigación se planteó como un análisis de la comunicación y los ges-tos de autocontacto en los comerciantes del Centro Comercial “Siete Colinas” en la ciudad de Guaranda en el período octubre 2018-febrero 2019”. Se diseñó como obje-tivo general determinar la influencia de la comunicación y gestos de autocontacto en el comportamiento de los comerciantes y consumidores a través de conceptualizar las variables involucradas, identificar el tipo de comunicación que se aplica en el proceso de comercialización y así mejorar el mismo. La conceptualización de la temática deja ver la importancia que tiene el tema en el diario vivir de los comerciantes, quienes no conocen la influencia de una buena comunicación sin exceder en gestos de autocontacto y una efectiva comunicación gestual para fidelizar a los clientes sobre la oferta de servicios y productos. Para conocer dichas deficiencias y fortalezas se utilizó una metodología de investigación aplicada, no experimental y bibliográfica apoyada por la aplicación de encuestas a los dueños de los locales, de quienes se obtuvo información relevante para emitir como conclusión que la comunicación gestual es parte importante en el proceso de compra y venta entre comerciantes y clientes, puesto que determina en la mayoría de los casos la confianza que se genera entre ellos para efectivizar la misma y así fidelizar a los clientes

Distinct roles of Arabidopsis ORC1 proteins in DNA replication and heterochromatic H3K27me1 deposition

13 Pág.Most cellular proteins involved in genome replication are conserved in all eukaryotic lineages including yeast, plants and animals. However, the mechanisms controlling their availability during the cell cycle are less well defined. Here we show that the Arabidopsis genome encodes for two ORC1 proteins highly similar in amino acid sequence and that have partially overlapping expression domains but with distinct functions. The ancestral ORC1b gene, present before the partial duplication of the Arabidopsis genome, has retained the canonical function in DNA replication. ORC1b is expressed in both proliferating and endoreplicating cells, accumulates during G1 and is rapidly degraded upon S-phase entry through the ubiquitin-proteasome pathway. In contrast, the duplicated ORC1a gene has acquired a specialized function in heterochromatin biology. ORC1a is required for efficient deposition of the heterochromatic H3K27me1 mark by the ATXR5/6 histone methyltransferases. The distinct roles of the two ORC1 proteins may be a feature common to other organisms with duplicated ORC1 genes and a major difference with animal cells.This research was supported by grants BFU2015-68396-R, RTI2018-094793-B-I00 (Ministerio de Ciencia e Innovacion) and AdG_833617 (European Research Council) to C.G., grant LABEX: ANR-10-LABX-0036-NETRNA and ANR-19-CE13-0032-01 to P.G. and S.N., and by institutional grants from Banco de Santander and Fundación Ramón Areces to the CBMSO.Peer reviewe

An outbreak of hantavirus pulmonary syndrome, Chile, 1997

Fil: Toro, Jorge. Ministry of Health; Chile.Fil: Vega, Jeanette D. Pan American Health Organization; Chile.Fil: Khan, Ali S. Centers for Disease Control and Prevention; Estados Unidos.Fil: Mills, James N. Centers for Disease Control and Prevention; Estados Unidos.Fil: Padula, Paula. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Terry, William. Centers for Disease Control and Prevention; Estados Unidos.Fil: Yadón, Zaida. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Valderrama, Rosa. Aysen Region XI Health Service; Chile.Fil: Ellis, Barbara A. Centers for Disease Control and Prevention; Estados Unidos.Fil: Pavletic, Carlos. Ministry of Health; Chile.Fil: Cerda, Rodrigo. Pan American Health Organization; Chile.Fil: Zaki, Sherif. Centers for Disease Control and Prevention; Estados Unidos.Fil: Wun-Ju, Shieh. Centers for Disease Control and Prevention; Estados Unidos.Fil: Meyer, Richard. Centers for Disease Control and Prevention; Estados Unidos.Fil: Tapia, Mauricio. Coyhaique Regional Hospital; Chile.Fil: Mansilla, Carlos. Coyhaique Regional Hospital; Chile.Fil: Baro, Michel. Llanchipal Health Services; Chile.Fil: Vergara, Jose A. Llanchipal Health Services; Chile.Fil: Concha, Marisol. Ministry of Health; Chile.Fil: Calderón, Gladys. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas Dr. Julio Maiztegui; Argentina.Fil: Enria, Delia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas Dr. Julio Maiztegui; Argentina.Fil: Peters, C.J. Centers for Disease Control and Prevention; Estados Unidos.Fil: Ksiazek, Thomas G. Centers for Disease Control and Prevention; Estados Unidos.An outbreak of 25 cases of Andes virus-associated hantavirus pulmonary syndrome (HPS) was recognized in southern Chile from July 1997 through January 1998. In addition to the HPS patients, three persons with mild hantaviral disease and one person with asymptomatic acute infection were identified. Epidemiologic studies suggested person-to-person transmission in two of three family clusters. Ecologic studies showed very high densities of several species of sigmodontine rodents in the area

An outbreak of hantavirus pulmonary syndrome, Chile, 1997

Fil: Toro, Jorge. Ministry of Health; Chile.Fil: Vega, Jeanette D. Pan American Health Organization; Chile.Fil: Khan, Ali S. Centers for Disease Control and Prevention; Estados Unidos.Fil: Mills, James N. Centers for Disease Control and Prevention; Estados Unidos.Fil: Padula, Paula. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Terry, William. Centers for Disease Control and Prevention; Estados Unidos.Fil: Yadón, Zaida. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Valderrama, Rosa. Aysen Region XI Health Service; Chile.Fil: Ellis, Barbara A. Centers for Disease Control and Prevention; Estados Unidos.Fil: Pavletic, Carlos. Ministry of Health; Chile.Fil: Cerda, Rodrigo. Pan American Health Organization; Chile.Fil: Zaki, Sherif. Centers for Disease Control and Prevention; Estados Unidos.Fil: Wun-Ju, Shieh. Centers for Disease Control and Prevention; Estados Unidos.Fil: Meyer, Richard. Centers for Disease Control and Prevention; Estados Unidos.Fil: Tapia, Mauricio. Coyhaique Regional Hospital; Chile.Fil: Mansilla, Carlos. Coyhaique Regional Hospital; Chile.Fil: Baro, Michel. Llanchipal Health Services; Chile.Fil: Vergara, Jose A. Llanchipal Health Services; Chile.Fil: Concha, Marisol. Ministry of Health; Chile.Fil: Calderón, Gladys. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas Dr. Julio Maiztegui; Argentina.Fil: Enria, Delia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas Dr. Julio Maiztegui; Argentina.Fil: Peters, C.J. Centers for Disease Control and Prevention; Estados Unidos.Fil: Ksiazek, Thomas G. Centers for Disease Control and Prevention; Estados Unidos.An outbreak of 25 cases of Andes virus-associated hantavirus pulmonary syndrome (HPS) was recognized in southern Chile from July 1997 through January 1998. In addition to the HPS patients, three persons with mild hantaviral disease and one person with asymptomatic acute infection were identified. Epidemiologic studies suggested person-to-person transmission in two of three family clusters. Ecologic studies showed very high densities of several species of sigmodontine rodents in the area

Azimuthal anisotropy of charged jet production in root s(NN)=2.76 TeV Pb-Pb collisions

We present measurements of the azimuthal dependence of charged jet production in central and semi-central root s(NN) = 2.76 TeV Pb-Pb collisions with respect to the second harmonic event plane, quantified as nu(ch)(2) (jet). Jet finding is performed employing the anti-k(T) algorithm with a resolution parameter R = 0.2 using charged tracks from the ALICE tracking system. The contribution of the azimuthal anisotropy of the underlying event is taken into account event-by-event. The remaining (statistical) region-to-region fluctuations are removed on an ensemble basis by unfolding the jet spectra for different event plane orientations independently. Significant non-zero nu(ch)(2) (jet) is observed in semi-central collisions (30-50% centrality) for 20 <p(T)(ch) (jet) <90 GeV/c. The azimuthal dependence of the charged jet production is similar to the dependence observed for jets comprising both charged and neutral fragments, and compatible with measurements of the nu(2) of single charged particles at high p(T). Good agreement between the data and predictions from JEWEL, an event generator simulating parton shower evolution in the presence of a dense QCD medium, is found in semi-central collisions. (C) 2015 CERN for the benefit of the ALICE Collaboration. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Peer reviewe

Production of He-4 and (4) in Pb-Pb collisions at root(NN)-N-S=2.76 TeV at the LHC

Results on the production of He-4 and (4) nuclei in Pb-Pb collisions at root(NN)-N-S = 2.76 TeV in the rapidity range vertical bar y vertical bar <1, using the ALICE detector, are presented in this paper. The rapidity densities corresponding to 0-10% central events are found to be dN/dy4(He) = (0.8 +/- 0.4 (stat) +/- 0.3 (syst)) x 10(-6) and dN/dy4 = (1.1 +/- 0.4 (stat) +/- 0.2 (syst)) x 10(-6), respectively. This is in agreement with the statistical thermal model expectation assuming the same chemical freeze-out temperature (T-chem = 156 MeV) as for light hadrons. The measured ratio of (4)/He-4 is 1.4 +/- 0.8 (stat) +/- 0.5 (syst). (C) 2018 Published by Elsevier B.V.Peer reviewe