Report of the Terrestrial Bodies Science Working Group. Volume 3: Venus

The science objectives of Pioneer Venus and future investigations of the planet are discussed. Concepts and payloads for proposed missions and the supporting research and technology required to obtain the desired measurements from space and Earth-based observations are examined, as well as mission priorities and schedules

Intra-molecular cross-linking of acidic residues for protein structure studies.

Intra-molecular cross-linking has been suggested as a method of obtaining distance constraints that would be useful in developing structural models of proteins. Recent work published on intra-molecular cross-linking for protein structural studies has employed commercially available primary amine selective reagents that can cross-link lysine residues to other lysine residues or the amino terminus. Previous work using these cross-linkers has shown that for several proteins of known structure, the number of cross-links that can be obtained experimentally may be small compared to what would be expected from the known structure, due to the relative reactivity, distribution, and solvent accessibility of the lysines in the protein sequence. To overcome these limitations we have investigated the use of cross-linking reagents that can react with other reactive sidechains in proteins. We used 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) to activate the carboxylic acid containing residues, aspartic acid (D), glutamic acid (E), and the carboxy terminus (O), for cross-linking reactions. Once activated, the DEO sidechains can react to form 'zero-length' cross-links with nearby primary amine containing resides, lysines (K) and the amino terminus (X), via the formation of a new amide bond. We also show that the EDC-activated DEO sidechains can be cross-linked to each other using dihydrazides, two hydrazide moieties connected by an alkyl cross-linker ann of variable length. Using these reagents, we have found three new 'zero-length' cross-links in ubiquitin consistent with its known structure (M1-E16, M1-E18, and K63-E64). Using the dihydrazide cross-linkers, we have identified 2 new cross-links (D21-D32 and E24-D32) unambiguously. Using a library of dihydrazide cross-linkers with varying arm length, we have shown that there is a minimum arm length required for the DEO-DEO cross-links of 5.8 angstroms. These results show that additional structural information can be obtained by exploiting new cross-linker chemistry, increasing the probability that the protein target of choice will yield sufficient distance constraints to develop a structural model

Optimizing an emperical scoring function for transmembrane protein structure determination.

We examine the problem of transmembrane protein structure determination. Like many other questions that arise in biological research, this problem cannot be addressed by traditional laboratory experimentation alone. An approach that integrates experiment and computation is required. We investigate a procedure which states the transmembrane protein structure determination problem as a bound constrained optimization problem using a special empirical scoring function, called Bundler, as the objective function. In this paper, we describe the optimization problem and some of its mathematical properties. We compare and contrast results obtained using two different derivative free optimization algorithms

The ATLAS3D project - XXIX : The new look of early-type galaxies and surrounding fields disclosed by extremely deep optical images

Date of Acceptance: 25/09/2014Galactic archaeology based on star counts is instrumental to reconstruct the past mass assembly of Local Group galaxies. The development of new observing techniques and data reduction, coupled with the use of sensitive large field of view cameras, now allows us to pursue this technique in more distant galaxies exploiting their diffuse low surface brightness (LSB) light. As part of the ATLAS3D project, we have obtained with the MegaCam camera at the Canada-France-Hawaii Telescope extremely deep, multiband images of nearby early-type galaxies (ETGs). We present here a catalogue of 92 galaxies from the ATLAS3D sample, which are located in low- to medium-density environments. The observing strategy and data reduction pipeline, which achieve a gain of several magnitudes in the limiting surface brightness with respect to classical imaging surveys, are presented. The size and depth of the survey are compared to other recent deep imaging projects. The paper highlights the capability of LSB-optimized surveys at detecting new prominent structures that change the apparent morphology of galaxies. The intrinsic limitations of deep imaging observations are also discussed, among those, the contamination of the stellar haloes of galaxies by extended ghost reflections, and the cirrus emission from Galactic dust. The detection and systematic census of fine structures that trace the present and past mass assembly of ETGs are one of the prime goals of the project. We provide specific examples of each type of observed structures - tidal tails, stellar streams and shells - and explain how they were identified and classified. We give an overview of the initial results. The detailed statistical analysis will be presented in future papers.Peer reviewedFinal Accepted Versio

Composite star formation histories of early-type galaxies from minor mergers: prospects for WFC3

The star formation history of nearby early-type galaxies is investigated via numerical modelling. Idealized hydrodynamical N-body simulations with a star formation prescription are used to study the minor merger process between a giant galaxy (host) and a less massive spiral galaxy (satellite) with reasonable assumptions for the ages and metallicities of the merger progenitors. We find that the evolution of the star formation rate is extended over several dynamical times and shows peaks which correspond to pericentre passages of the satellite. The newly formed stars are mainly located in the central part of the satellite remnant while the older stars of the initial disk are deposited at larger radii in shell-like structures. After the final plunge of the satellite, star formation in the central part of the remnant can continue for several Gyrs depending on the star formation efficiency. Although the mass fraction in new stars is small, we find that the half-mass radius differs from the half-light radius in the V and H bands. Moreover synthetic 2D images in J, H, NUV, Hb and V bands, using the characteristic filters of the Wide Field Camera 3 (WFC3) on the Hubble Space Telescope (HST), reveal that residual star formation induced by gas-rich minor mergers can be clearly observed during and after the final plunge, especially in the NUV band, for interacting systems at (z<0.023) over moderate numbers of orbits (~2 orbits correspond to typical exposure times of ~3600 sec). This suggests that WFC3 has the potential to resolve these substructures, characterize plausible past merger episodes, and give clues to the formation of early-type galaxies.Comment: accepted for publication in MNRAS - moderate changes from the first versio

Ambiguous loss and incomplete abduction narratives in Kosovo

Ten mothers of men and boys who were abducted and listed as missing during the war in Kosovo in 1998/1999 were interviewed in Kosovo in the spring of 2012. Although the missing are presumed dead by the authorities, the mothers continue to live in a state of emotional ambiguity where a presumption of death is balanced with the hope of being reunited. In the absence of absolute proof, finding the remains of their loved ones becomes a major preoccupation. Using a social phenomenological approach, this study explored the social and political complexities existing within the life-world of these women. The findings suggest that they live in a continual state of psychological distress, and even when remains are returned, the unknown elements of the narrative of their abduction and murder only add to their distress and force many into self-imposed emotional exile away from community and close family

Optical line profiles of the Helix planetary nebula (NGC 7293) to large radii

New, very long (25'), cuts of spatially resolved profiles of the Halpha and [N II] optical emission lines have been obtained over the face of the Helix planetary nebula, NGC 7293. These directions were chosen to supplement previous similar, though shorter, cuts as well as crossing interesting phenomena in this nebular envelope. In particular one new cut crosses the extremes of the proposed CO J=2-1 emitting outer "torus" shown by Huggins and his co-workers to be nearly orthogonal to its inner counterpart. The second new cut crosses the extensive outer filamentary arcs on either side of the bright nebular core. It is shown that NGC 7293 is composed of multiple bipolar outflows along different axes. Hubble-type outflows over a dynamical timescale of 11,000 years are shown to be occurring for all the phenomena from the smallest He II emitting core out to the largest outer filamentary structure. All must then have been ejected over a short timescale but with a range of ejection velocitiesComment: 14 pages, 7 figures, MNRAS in pres

Introduction to special section on the Phoenix Mission: Landing Site Characterization Experiments, Mission Overviews, and Expected Science

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/94752/1/jgre2486.pd

Chemical crosslinking and mass spectrometry studies of the structure and dynamics of membrane proteins and receptors.

Membrane proteins make up a diverse and important subset of proteins for which structural information is limited. In this study, chemical cross-linking and mass spectrometry were used to explore the structure of the G-protein-coupled photoreceptor bovine rhodopsin in the dark-state conformation. All experiments were performed in rod outer segment membranes using amino acid 'handles' in the native protein sequence and thus minimizing perturbations to the native protein structure. Cysteine and lysine residues were covalently cross-linked using commercially available reagents with a range of linker arm lengths. Following chemical digestion of cross-linked protein, cross-linked peptides were identified by accurate mass measurement using liquid chromatography-fourier transform mass spectrometry and an automated data analysis pipeline. Assignments were confirmed and, if necessary, resolved, by tandem MS. The relative reactivity of lysine residues participating in cross-links was evaluated by labeling with NHS-esters. A distinct pattern of cross-link formation within the C-terminal domain, and between loop I and the C-terminal domain, emerged. Theoretical distances based on cross-linking were compared to inter-atomic distances determined from the energy-minimized X-ray crystal structure and Monte Carlo conformational search procedures. In general, the observed cross-links can be explained by re-positioning participating side-chains without significantly altering backbone structure. One exception, between C3 16 and K325, requires backbone motion to bring the reactive atoms into sufficient proximity for cross-linking. Evidence from other studies suggests that residues around K325 for a region of high backbone mobility. These findings show that cross-linking studies can provide insight into the structural dynamics of membrane proteins in their native environment