Arthropods of the Limarí River basin (Coquimbo Region, Chile): taxonomic composition in agricultural ecosystems

The Limarí valley, located in the Coquimbo Region of Chile, is an important agricultural area that is immersed in the transverse valleys of the Norte Chico. In recent decades, the continuous expansion of agriculture towards dry land zones has favored the migration and establishment of potential pests, such as arthropods, that may affect crops or be zoonotic agents. Based on the limited knowledge we have about the arthropod group present in the Limarí basin, our objective is to describe the taxonomic composition of the assemblage of economically important arthropods inhabiting this basin of the semiarid region of Chile. After reviewing historical data, specimen collections, and the specialized literature, a total of 414 arthropod species were recorded. Of the total number of species recorded, 92.5% were insects, the most diverse taxon, with 11 orders. Arachnids, in turn, were represented only by Acari with 31 species. The most widely represented orders of insects were Coleoptera, Hemiptera, and Lepidoptera. Within Coleoptera the most species-rich families were, in decreasing order of importance, Curculionidae, Coccinellidae, Cerambycidae, Scarabaeidae, Chrysomelidae (Bruchinae), Ptinidae, and Bostrichidae; within Hemiptera these were Aphididae, Diaspididae, Coccidae, Pseudococcidae, Pentatomidae and Rhopalidae; and within Lepidoptera they were Noctuidae and Tortricidae. We hope this study serves as a starting point for identifying the most diverse arthropod groups and developing pest monitoring and control programs. Highlights: A large percentage of phytophagous species, mainly belonging to Acari, Lepidoptera, Hemiptera and Coleoptera, were registered in the Limarí basin. Some families of agricultural importance (Aleyrodidae, Aphididae, Coccidae, Diaspididae, Margarodidae, Pseudococcidae), were observed in large agricultural crops in the basin (e.g., vines, oranges, mandarins, lemon trees, avocado trees, walnuts, olive trees, vegetable crops). A smaller fraction corresponded to the group of predators and parasitoids, mainly represented by Coleoptera (Coccinellidae), Neuroptera (Chrysopidae) and Hymenoptera (Braconidae, Encyrtidae, Ichneumonidae, Platygastridae, Signiphoridae). The richness and spatial records of arthropods were mostly concentrated between the city of Ovalle and the estuary of Punitaqui - the areas with most intense agricultural activity in the Limarí basin.The Limarí valley, located in the Coquimbo Region of Chile, is an important agricultural area that is immersed in the transverse valleys of the Norte Chico. In recent decades, the continuous expansion of agriculture towards dry land zones has favored the migration and establishment of potential pests, such as arthropods, that may affect crops or be zoonotic agents. Based on the limited knowledge we have about the arthropod group present in the Limarí basin, our objective is to describe the taxonomic composition of the assemblage of economically important arthropods inhabiting this basin of the semiarid region of Chile. After reviewing historical data, specimen collections, and the specialized literature, a total of 414 arthropod species were recorded. Of the total number of species recorded, 92.5% were insects, the most diverse taxon, with 11 orders. Arachnids, in turn, were represented only by Acari with 31 species. The most widely represented orders of insects were Coleoptera, Hemiptera, and Lepidoptera. Within Coleoptera the most species-rich families were, in decreasing order of importance, Curculionidae, Coccinellidae, Cerambycidae, Scarabaeidae, Chrysomelidae (Bruchinae), Ptinidae, and Bostrichidae; within Hemiptera these were Aphididae, Diaspididae, Coccidae, Pseudococcidae, Pentatomidae and Rhopalidae; and within Lepidoptera they were Noctuidae and Tortricidae. We hope this study serves as a starting point for identifying the most diverse arthropod groups and developing pest monitoring and control programs. Highlights: A large percentage of phytophagous species, mainly belonging to Acari, Lepidoptera, Hemiptera and Coleoptera, were registered in the Limarí basin. Some families of agricultural importance (Aleyrodidae, Aphididae, Coccidae, Diaspididae, Margarodidae, Pseudococcidae), were observed in large agricultural crops in the basin (e.g., vines, oranges, mandarins, lemon trees, avocado trees, walnuts, olive trees, vegetable crops). A smaller fraction corresponded to the group of predators and parasitoids, mainly represented by Coleoptera (Coccinellidae), Neuroptera (Chrysopidae) and Hymenoptera (Braconidae, Encyrtidae, Ichneumonidae, Platygastridae, Signiphoridae). The richness and spatial records of arthropods were mostly concentrated between the city of Ovalle and the estuary of Punitaqui - the areas with most intense agricultural activity in the Limarí basin

Influence of IFN-gamma and its receptors in human breast cancer

<p>Abstract</p> <p>Background</p> <p>Interferons are a group of proteins that trigger multiple responses including prevention of viral replication, inhibition of cell growth, and modulation of cell differentiation. In different mammary carcinoma cell lines IFNγ induces growth arrest at mid-G1. At the present there are no <it>in vivo </it>studies in human breast. The aim of this study was to investigate the expression patterns of IFNγ and its two receptors (IFNγ-Rα and IFNγ-Rβ) by Western blot and immunohistochemistry, in order to elucidate its role in the different types of human breast cancer (<it>in situ </it>and infiltrative).</p> <p>Methods</p> <p>Immunohistochemical and semiquantitative study of IFNγ, its receptors types (IFNγ-Rα and IFNγ-Rβ), cell proliferation (proliferating cell nuclear antigen, also named PCNA), and apoptosis (TUNEL method) was carried between the three breast groups (fibrocystic lesions, <it>in situ</it> tumors and infiltrating tumors).</p> <p>Results</p> <p>In the three groups of patients, IFNγ and IFNγ-Rα immunoreactions appeared in the cytoplasm while IFNγ-Rβ also was found in the nucleus. The optical density to IFNγ was higher in <it>in situ </it>carcinoma than in benign and infiltrating tumors. When we observed IFNγ-Rα, the optical density was lower in infiltrating carcinoma than in benign and <it>in situ </it>tumors (the higher density). To IFNγ-Rβ, the optical density was similar in the three group samples. In tumor samples PCNA and TUNEL index was significantly higher; than in benign diseases. PCNA index increased with the malignance. No significant differences were found between cancer types to TUNEL. IFNγ could be a potential therapeutic tool in breast cancer. However, tumor cells are able to escape from the control of this cytokine in the early tumor stages; this is probably due to a decreased expression of IFNγ, or also to an alteration of either its receptors or some transduction elements.</p> <p>Conclusion</p> <p>We conclude that the decrease in the % positive samples that expressed IFNγ and IFNγ-Rα together with the nuclear localization of IFNγ-Rβ, could be a tumoral cell response, although perhaps insufficient to inhibit the uncontrolled cell proliferation. Perhaps, IFNγ might be unable to activate p21 to stop the cell cycle, suggesting a possible participation in breast cancer development.</p

The interplay between ionized gas and massive stars in the HII galaxy IIZw70: integral field spectroscopy with PMAS

We performed an integral field spectroscopic study for the HII galaxy IIZw70 in order to investigate the interplay between its ionized interstellar medium (ISM) and the massive star formation (SF). Observations were taken in the optical spectral range (3700-6800 A) with the Potsdam Multi-Aperture Spectrophotometer (PMAS) attached to the 3.5 m telescope at CAHA. We created and analysed maps of spatially distributed emission-lines, continuum emission and properties of the ionized ISM (e.g. physical-chemical conditions, dust extinction, kinematics). We investigated the relation of these properties to the spatial distribution and evolutionary stage of the massive stars. For the first time we have detected the presence of Wolf-Rayet (WR) stars in this galaxy. The peak of the ionized gas emission coincides with the location of the WR bump. The region of the galaxy with lower dust extinction corresponds to the region that shows the lowest values of velocity dispersion and radial velocity. The overall picture suggests that the ISM of this region is being disrupted via photoionization and stellar winds, leading to a spatial decoupling between gas+stars and dust clouds. The bulk of dust appears to be located at the boundaries of the region occupied by the probable ionizing cluster. We also found that this region is associated to the nebular emission in HeII4686 and to the intensity maximum of most emission lines. This indicates that the hard ionizing radiation responsible for the HeII4686 nebular emission can be related to the youngest stars. Within $\sim$ 0.4 x 0.3 kpc^2 in the central burst, we derived O/H using direct determinations of Te[OIII]. We found abundances in the range 12+log(O/H)=7.65-8.05, yielding an error-weighted mean of 12+log(O/H)=7.86 $\pm$0.05.Comment: 10 pages, 10 figures, accepted for publication in A&A, minor changes adde

Cardiotrophin-1 promotes a high survival rate in rabbits with lethal fulminant hepatitis of viral origin

Rabbit hemorrhagic disease virus (RHDV) causes lethal fulminant hepatitis closely resembling acute liver failure (ALF) in humans. In this study, we investigated whether cardiotrophin-1 (CT-1), a cytokine with hepatoprotective properties, could attenuate liver damage and prolong survival in virus-induced ALF. Twenty-four rabbits were infected with 2 × 10(4) hemagglutination units of RHDV. Twelve received five doses of CT-1 (100 μg/kg) starting at 12 h postinfection (hpi) (the first three doses every 6 h and then two additional doses at 48 and 72 hpi), while the rest received saline. The animals were analyzed for survival, serum biochemistry, and viral load. Another cohort (n = 22) was infected and treated similarly, but animals were sacrificed at 30 and 36 hpi to analyze liver histology, viral load, and the expression of factors implicated in liver damage and repair. All infected rabbits that received saline died by 60 hpi, while 67% of the CT-1-treated animals survived until the end of the study. Treated animals showed improved liver function and histology, while the viral loads were similar. In the livers of CT-1-treated rabbits we observed reduction of oxidative stress, diminished PARP1/2 and JNK activation, and decreased inflammatory reaction, as reflected by reduced expression of tumor necrosis factor alpha, interleukin-1β, Toll-like receptor 4, VCAM-1, and MMP-9. In addition, CT-1-treated rabbits exhibited marked upregulation of TIMP-1 and increased expression of cytoprotective and proregenerative growth factors, including platelet-derived growth factor B, epidermal growth factor, platelet-derived growth factor receptor β, and c-Met. In conclusion, in a lethal form of acute viral hepatitis, CT-1 increases animal survival by attenuating inflammation and activating cytoprotective mechanisms, thus representing a promising therapy for ALF of viral origin

Experiencias interdisciplinarias en alimentación y daños a la salud en las fronteras sur y norte de México

Los problemas de salud y alimentación en diferentes contextos como las fronteras sur y norte de México exige de trabajo interdisciplinar, con lenguajes y bases comunes para los grupos académicos. El objetivo de esta ponencia es reflexionar sobre la experiencia del grupo Seminario de “Alimentación y Daños a la Salud” (ADAS), el cual busca consolidar una propuesta de colaboración interinstitucional para el abordaje interdisciplinario de los problemas de salud relacionados con la alimentación y vulnerabilidad social en la frontera sur y norte de México.Facultad de Humanidades y Ciencias de la Educació

Experiencias interdisciplinarias en alimentación y daños a la salud en las fronteras sur y norte de México

Los problemas de salud y alimentación en diferentes contextos como las fronteras sur y norte de México exige de trabajo interdisciplinar, con lenguajes y bases comunes para los grupos académicos. El objetivo de esta ponencia es reflexionar sobre la experiencia del grupo Seminario de “Alimentación y Daños a la Salud” (ADAS), el cual busca consolidar una propuesta de colaboración interinstitucional para el abordaje interdisciplinario de los problemas de salud relacionados con la alimentación y vulnerabilidad social en la frontera sur y norte de México.Facultad de Humanidades y Ciencias de la Educació

Therapeutic impact of cytoreductive surgery and irradiation of posterior fossa ependymoma in the molecular era: a retrospective multicohort analysis

PURPOSE: Posterior fossa ependymoma comprises two distinct molecular variants termed EPN_PFA and EPN_PFB that have a distinct biology and natural history. The therapeutic value of cytoreductive surgery and radiation therapy for posterior fossa ependymoma after accounting for molecular subgroup is not known. METHODS: Four independent nonoverlapping retrospective cohorts of posterior fossa ependymomas (n = 820) were profiled using genome-wide methylation arrays. Risk stratification models were designed based on known clinical and newly described molecular biomarkers identified by multivariable Cox proportional hazards analyses. RESULTS: Molecular subgroup is a powerful independent predictor of outcome even when accounting for age or treatment regimen. Incompletely resected EPN_PFA ependymomas have a dismal prognosis, with a 5-year progression-free survival ranging from 26.1% to 56.8% across all four cohorts. Although first-line (adjuvant) radiation is clearly beneficial for completely resected EPN_PFA, a substantial proportion of patients with EPN_PFB can be cured with surgery alone, and patients with relapsed EPN_PFB can often be treated successfully with delayed external-beam irradiation. CONCLUSION: The most impactful biomarker for posterior fossa ependymoma is molecular subgroup affiliation, independent of other demographic or treatment variables. However, both EPN_PFA and EPN_PFB still benefit from increased extent of resection, with the survival rates being particularly poor for subtotally resected EPN_PFA, even with adjuvant radiation therapy. Patients with EPN_PFB who undergo gross total resection are at lower risk for relapse and should be considered for inclusion in a randomized clinical trial of observation alone with radiation reserved for those who experience recurrence

Therapeutic Impact of Cytoreductive Surgery and Irradiation of Posterior Fossa Ependymoma in the Molecular Era: A Retrospective Multicohort Analysis

Posterior fossa ependymoma comprises two distinct molecular variants termed EPN_PFA and EPN_PFB that have a distinct biology and natural history. The therapeutic value of cytoreductive surgery and radiation therapy for posterior fossa ependymoma after accounting for molecular subgroup is not known

Microvesicles in vascular homeostasis and diseases. Position Paper of the European Society of Cardiology (ESC) Working Group on Atherosclerosis and Vascular Biology

Microvesicles are members of the family of extracellular vesicles shed from the plasma membrane of activated or apoptotic cells. Microvesicles were initially characterised by their pro-coagulant activity and described as "microparticles". There is mounting evidence revealing a role for microvesicles in intercellular communication, with particular relevance to hemostasis and vascular biology. Coupled with this, the potential of microvesicles as meaningful biomarkers is under intense investigation. This Position Paper will summarise the current knowledge on the mechanisms of formation and composition of microvesicles of endothelial, platelet, red blood cell and leukocyte origin. This paper will also review and discuss the different methods used for their analysis and quantification, will underline the potential biological roles of these vesicles with respect to vascular homeostasis and thrombosis and define important themes for future research