LA GESTIÓN DE PROYECTOS DENTRO DEL PROCESO DE DESARROLLO SOFTWARE

ResumenEste artículo contiene los resultados del estudio de algunos elementos de la ingeniería del software(un componente conceptual, base de la ingeniería, entre los cuales están los estándares, las buenas practicas, metodologías, técnicas y herramientas), desde el punto de vista de la gestión de proyectos. Los elementos de estudio seleccionados incluyen estándares sobre el proceso de desarrollo (IEEE Std 1074, IEEE/EIA 12207), prácticas de ingeniería del software (CMM) y metodologías de desarrollo software (Proceso Unificado, METRICA V3 y Programación Extrema). Los factores de la gestión de proyectos incluidos en el análisis son: la estimación, planificación, seguimiento y control del proyecto, gestión de configuración, gestión de calidad y gestión de personal.Palabras ClaveGestión de proyectos, Ingeniería del software, Proceso dedesarrollo softwar

Is employment status in adults over 25 years old associated with nonmedical prescription opioid and stimulant use?

Purpose: Nonmedical use of prescription opioid and stimulants (NMUPO and NMUPS, respectively) has declined in recent years, but remains an important public health problem. Evidence regarding their relationships with employment status remains unclear. We determined the relationship between employment status and NMUPO and NMUPS. Methods: We analyzed a cross-sectional, nationally representative, weighted sample of 58,486 adults, ages 26 years and older, using combined 2011–2013 data from the National Survey on Drug Use and Health (NSDUH). We fit two crude and two adjusted multivariable logistic regression models to assess the relationship between our two different outcomes of interest: (1) past-year NMUPO and (2) past-year NMUPS, and our exposure of interest: employment status, categorized as (1) full time, (2) part time, (3) unemployed, and (4) not in the workforce. Our adjusted models featured the following covariates: sex, race, age, marital status, and psychological distress, and other nonmedical use. Results: Prevalence of NMUPO was higher than NMUPS (3.48 vs. 0.72%). Unemployed participants had the highest odds of NMUPO [aOR 1.45, 95% CI (1.15–1.82)], while those not in the workforce had the highest odds of NMUPS [aOR 1.71, 95% CI (1.22–2.37)]. Additionally, part-time and unemployed individuals had increased odds of NMUPS [aORs, 95% CI 1.59 (1.09–2.31) and 1.67 (1.11–2.37) respectively], while those not in the workforce had decreased odds of NMUPO [aOR 0.82, 95% CI (0.68–0.99)] relative to full-time participants. Conclusions: There is a need for adult prevention and deterrence programs that target nonmedical prescription drug use, especially among those unemployed or not in the workforce

Vitamin C enhances NF-κB-driven epigenomic reprogramming and boosts the immunogenic properties of dendritic cells

Dendritic cells (DCs), the most potent antigen-presenting cells, are necessary for effective activation of naïve T cells. DCs' immunological properties are modulated in response to various stimuli. Active DNA demethylation is crucial for DC differentiation and function. Vitamin C, a known cofactor of ten-eleven translocation (TET) enzymes, drives active demethylation. Vitamin C has recently emerged as a promising adjuvant for several types of cancer; however, its effects on human immune cells are poorly understood. In this study, we investigate the epigenomic and transcriptomic reprogramming orchestrated by vitamin C in monocyte-derived DC differentiation and maturation. Vitamin C triggers extensive demethylation at NF-κB/p65 binding sites, together with concordant upregulation of antigen-presentation and immune response-related genes during DC maturation. p65 interacts with TET2 and mediates the aforementioned vitamin C-mediated changes, as demonstrated by pharmacological inhibition. Moreover, vitamin C increases TNFβ production in DCs through NF-κB, in concordance with the upregulation of its coding gene and the demethylation of adjacent CpGs. Finally, vitamin C enhances DC's ability to stimulate the proliferation of autologous antigen-specific T cells. We propose that vitamin C could potentially improve monocyte-derived DC-based cell therapies

Neck circumference and clustered cardiovascular risk factors in children and adolescents: Cross-sectional study.

OBJECTIVE: Early detection of cardiovascular disease (CVD) risk factors, such as obesity, is crucial to prevent adverse long-term effects on individuals' health. Therefore, the aims were: (1) to explore the robustness of neck circumference (NC) as a predictor of CVD and examine its association with numerous anthropometric and body composition indices and (2) to release sex and age-specific NC cut-off values to classify youths as overweight/obese. DESIGN: Cross-sectional study. SETTING: 23 primary schools and 17 secondary schools from Spain. PARTICIPANTS: 2198 students (1060 girls), grades 1-4 and 7-10. MEASURES: Pubertal development, anthropometric and body composition indices, systolic and diastolic blood pressure (SBP and DBP, respectively), cardiorespiratory fitness, blood sampling triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), glucose and inflammatory markers. Homoeostasis model assessment (HOMA-IR) and cluster of CVD risk factors were calculated. RESULTS: NC was negatively associated with maximum oxygen consumption (R2=0.231, P<0.001 for boys; R2=0.018, P<0.001 for girls) and adiponectin (R2=0.049, P<0.001 for boys; R2=0.036, P<0.001 for girls); and positively associated with SBP, DBP, TC/HDL-c, TG, HOMA, complement factors C-3 and C-4, leptin and clustered CVD risk factor in both sexes (R2 from 0.035 to 0.353, P<0.01 for boys; R2 from 0.024 to 0.215, P<0.001 for girls). Moreover, NC was positively associated with serum C reactive protein and LDL-c only in boys (R2 from 0.013 to 0.055, P<0.05). CONCLUSION: NC is a simple, low-cost and practical screening tool of excess of upper body obesity and CVD risk factors in children and adolescents. Paediatricians can easily use it as a screening tool for overweight/obesity in children and adolescents. For this purpose, sex and age-specific thresholds to classify children and adolescents as normal weight or overweight/obese are provided.This work was supported by the DEP 2010 21662 C04 00 (DEP 2010 21662 C04 01: DEP 2010 21662 C04 02: DEP 2010 21662 C04 03: DEP 2010 21662 C04 04) grant from the National Plan for Research: Development and Innovation (R+D+i) MICINN

Prescription opioid use disorder and heroin use among 12-34 year-olds in the United States from 2002 to 2014

Trend analyses of prescription opioids in the U.S. indicate use, especially use of prescription opioids stronger than morphine, has more than doubled among adults since the early 1990's (Frenk, Porter, & Paulozzi, 2015). Prescription opioids, like Oxycontin®, are effective pharmacological treatments for acute and chronic pain (Fitzcharles and Shir, 2009 ; Gallagher and Rosenthal, 2008). When used as indicated, these medications can be an important component of pain management. However, their high abuse potential presents concerns regarding their nonmedical use, which can be defined as ‘use of a prescription opioid that was not prescribed, or taken for the experience or feeling it caused’ (SAMHSA, 2014). In the United States, nonmedical use of prescription opioids (NMPO) is increasingly recognized as a serious public health problem among adults (Blanco et al., 2007; Han et al., 2015 ; Huang et al., 2006). Nonmedical prescription drug use, specifically nonmedical use of prescription opioids, is also a growing problem in other countries such as Canada (Fischer et al., 2014 ; Fischer et al., 2013) and Australia (Degenhardt et al., 2006 ; Rintoul et al., 2011)

Proyecto Como en casa

El presente proyecto consiste en el desarrollo de un emprendimiento digital que ofrece comida casera y permite que amas de casa, con gran talento para cocinar, puedan conectarse con un grupo importante de clientes. Asimismo, se muestra la viabilidad de su implementación en base a la investigación que realizamos en los distritos de Lima Moderna. Esto nos permitió detectar que los jóvenes desean una alternativa distinta a la que ofrecen los aplicativos de delivery, en su mayoría comida procesada y de esta forma poder obtener una comida casera y balanceada. Además, se identificó un segmento de amas de casa que tienen el deseo de incrementar sus ingresos, que han sido afectadas en el aspecto económico por la pandemia y que además tienen grandes habilidades culinarias que pueden aprovechar. Para la implementación del proyecto previamente se realizó un análisis del mercado potencial y se elaboraron experimentos para validar las partes del modelo de negocio: canales, segmentos de clientes, relación con los clientes, actividades clave, compañeros claves, estructura de costos y las fuentes de ingreso. Por otro lado, se elaboró un Plan de marketing, Plan de fidelización y un Plan Financiero. Los cuales tienen como finalidad reafirmar la viabilidad del proyecto. Como resultado, luego de una inversión inicial de 15 475 soles en el primer año de operaciones se obtendrá una utilidad neta de 5 600 soles, 65.000 soles para el segundo año y 144 000 soles para el tercer año.This project consists of the development of a digital entrepreneurship that offers homemade food and allows housewives, with great cooking talent, to connect with an important group of clients. Likewise, the feasibility of its implementation is shown based on the research we carried out in the districts of Modern Lima. This allowed us to detect that young people want a different alternative to the one offered by delivery applications, mostly processed food, and in this way to be able to obtain a homemade and balanced meal. In addition, a segment of housewives was identified who have the desire to increase their income, who have been affected economically by the pandemic, and who also have great culinary skills that they can take advantage of. For the implementation of the project, an analysis of the potential market was previously carried out and experiments were developed to validate the parts of the business model: channels, customer segments, relationship with customers, key activities, key partners, cost structure, and sources of entry. On the other hand, a Marketing Plan, a Loyalty Plan, and a Financial Plan were drawn up. Which are intended to reaffirm the viability of the project. As a result, after an initial investment of 11,993 soles in the first year of operations, a net profit of 5,600 soles will be obtained, 65,000 soles for the second year, and 144,000 soles for the third year.Trabajo de investigació

Comparação de pH e matéria seca em três processos de silagem com forragem de gergelim (Sesamum indicum) nos trópicos secos.

El objetivo de este estudio fue comparar el pH y la materia seca (MS) en tres procesos de ensilaje con planta de ajonjolí en climas secos. Los experimentos se realizaron en la Facultad de Medicina Veterinaria y Zootecnia No. 1 de la Universidad Autónoma de Guerrero. En el experimento 1, se ensiló forraje de maíz combinado con forraje de ajonjolí (T1: 100:00, T2: 75:25, T3: 50:50, T4: 25:75 y T5: 00:100% respectivamente). En el experimento 2 se ensiló forraje de ajonjolí mezclado con melaza (T1:0, T2:4, T3:8 y T4:12% respectivamente) En el experimento 3 se ensiló planta de ajonjolí sola (T1), con melaza 8% (T2), planta presecada sola (T3) y planta presecada con melaza 8% (T4). Se realizaron síes microsilos por tratamiento (6 réplicas). Los diseños experimentales fueron completamente al azar. En el experimento 1, el pH más bajo fue para el T1 con un valor de 3.83, en el experimento 2 fue para el T4 con un valor de 3.94 y en el experimento 3 fue para el T2 con un valor de 4.00. Para la MS, el contenido fue mayor para el T1 con 28.55%, en el experimento 2 fue para el T4 con 27.63% y en el experimento 3 fue para el T3 con 36.00%. Todos los experimentos mostraron diferencia estadística (p&lt; 0.0001) entre tratamientos. Sin embargo, los valores para pH y MS son aceptables para prácticamente todos los tratamientos, en acuerdo con otras investigaciones. Por lo tanto, se concluye que al ensilar forraje de ajonjolí mezclado con forraje de maíz, melaza o planta presercada se logra mejorar la fermentación ácida y se incrementa el contenido de materia seca. Sin embargo, es necesario realizar evaluaciones posteriores de las propiedades físico-químicas y determinar su valor nutritivo.The objective of this study was to compare the pH and dry matter (DM) in three silage processes with sesame plants in dry climates. The experiments were carried out at the Faculty of Veterinary Medicine and Zootechnics No. 1 of the Autonomous University of Guerrero. In experiment 1, corn forage combined with sesame forage was ensiled (T1: 100:00, T2: 75:25, T3: 50:50, T4: 25:75 and T5: 00:100% respectively). In experiment 2 sesame forage mixed with molasses (T1:0, T2:4, T3:8 and T4:12% respectively) was ensiled. In experiment 3 sesame plant alone (T1) was ensiled with 8% molasses ( T2), pre-dried plant alone (T3) and pre-dried plant with 8% molasses (T4). Six microsilos per treatment (6 replicates) were made. The experimental designs were completely randomized. In experiment 1, the lowest pH was for T1 with a value of 3.83, in experiment 2 it was for T4 with a value of 3.94 and in experiment 3 it was for T2 with a value of 4.00. For DM, the content was higher for T1 with 28.55%, in experiment 2 it was for T4 with 27.63% and in experiment 3 it was for T3 with 36.00%. All the experiments showed statistical difference (p&lt; 0.0001) between treatments. However, the values for pH and DM are acceptable for practically all treatments, in agreement with other investigations. Therefore, it is concluded that ensiling sesame forage mixed with corn forage, molasses or presercated plant improves acid fermentation and increases the dry matter content. However, it is necessary to carry out further evaluations of the physical-chemical properties and determine its nutritive value.O objetivo deste estudo foi comparar o pH e a matéria seca (MS) em três processos de silagem com plantas de gergelim em climas secos. Os experimentos foram realizados na Faculdade de Medicina Veterinária e Zootecnia nº 1 da Universidade Autônoma de Guerrero. No experimento 1, a forragem de milho combinada com a forragem de gergelim foi ensilada (T1: 100:00, T2: 75:25, T3: 50:50, T4: 25:75 e T5: 00:100% respectivamente). No experimento 2 a forragem de gergelim foi ensilada misturada com melaço (T1:0, T2:4, T3:8 e T4:12% respectivamente) - planta seca sozinha (T3) e planta pré-seca com 8% de melaço (T4). Seis microsilos por tratamento (6 réplicas) foram feitos. Os delineamentos experimentais foram inteiramente casualizados. No experimento 1, o menor pH foi para T1 com valor de 3,83, no experimento 2 foi para T4 com valor de 3,94 e no experimento 3 foi para T2 com valor de 4,00. Para MS o teor foi maior para T1 com 28,55%, no experimento 2 foi para T4 com 27,63% e no experimento 3 foi para T3 com 36,00%. Todos os experimentos apresentaram diferença estatística (p&lt; 0,0001) entre os tratamentos. No entanto, os valores de pH e MS são aceitáveis ​​para praticamente todos os tratamentos, concordando com outras investigações. Portanto, conclui-se que a ensilagem de forragem de gergelim misturada com forragem de milho, melaço ou planta preservada melhora a fermentação ácida e aumenta o teor de matéria seca. No entanto, é necessário realizar mais avaliações das propriedades físico-químicas e determinar seu valor nutritivo

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Impact of the first wave of the SARS-CoV-2 pandemic on the outcome of neurosurgical patients: A nationwide study in Spain

Objective To assess the effect of the first wave of the SARS-CoV-2 pandemic on the outcome of neurosurgical patients in Spain. Settings The initial flood of COVID-19 patients overwhelmed an unprepared healthcare system. Different measures were taken to deal with this overburden. The effect of these measures on neurosurgical patients, as well as the effect of COVID-19 itself, has not been thoroughly studied. Participants This was a multicentre, nationwide, observational retrospective study of patients who underwent any neurosurgical operation from March to July 2020. Interventions An exploratory factorial analysis was performed to select the most relevant variables of the sample. Primary and secondary outcome measures Univariate and multivariate analyses were performed to identify independent predictors of mortality and postoperative SARS-CoV-2 infection. Results Sixteen hospitals registered 1677 operated patients. The overall mortality was 6.4%, and 2.9% (44 patients) suffered a perioperative SARS-CoV-2 infection. Of those infections, 24 were diagnosed postoperatively. Age (OR 1.05), perioperative SARS-CoV-2 infection (OR 4.7), community COVID-19 incidence (cases/10 5 people/week) (OR 1.006), postoperative neurological worsening (OR 5.9), postoperative need for airway support (OR 5.38), ASA grade =3 (OR 2.5) and preoperative GCS 3-8 (OR 2.82) were independently associated with mortality. For SARS-CoV-2 postoperative infection, screening swab test <72 hours preoperatively (OR 0.76), community COVID-19 incidence (cases/10 5 people/week) (OR 1.011), preoperative cognitive impairment (OR 2.784), postoperative sepsis (OR 3.807) and an absence of postoperative complications (OR 0.188) were independently associated. Conclusions Perioperative SARS-CoV-2 infection in neurosurgical patients was associated with an increase in mortality by almost fivefold. Community COVID-19 incidence (cases/10 5 people/week) was a statistically independent predictor of mortality. Trial registration number CEIM 20/217

Dendritic Cell-Mediated Cross-Priming by a Bispecific Neutralizing Antibody Boosts Cytotoxic T Cell Responses and Protects Mice against SARS-CoV-2

17 p.-4 fig.Administration of neutralizing antibodies (nAbs) has proved to be effective by providing immediate protection against SARS-CoV-2. However, dual strategies combining virus neutralization and immune response stimulation to enhance specific cytotoxic T cell responses, such as dendritic cell (DC) cross-priming, represent a promising field but have not yet been explored. Here, a broadly nAb, TNT, are first generated by grafting an anti-RBD biparatopic tandem nanobody onto a trimerbody scaffold. Cryo-EM data show that the TNT structure allows simultaneous binding to all six RBD epitopes, demonstrating a high-avidity neutralizing interaction. Then, by C-terminal fusion of an anti-DNGR-1 scFv to TNT, the bispecific trimerbody TNTDNGR-1 is generated to target neutralized virions to type 1 conventional DCs (cDC1s) and promote T cell cross-priming. Therapeutic administration of TNTDNGR-1, but not TNT, protects K18-hACE2 mice from a lethal SARS-CoV-2 infection, boosting virus-specific humoral responses and CD8+ T cell responses. These results further strengthen the central role of interactions with immune cells in the virus-neutralizing antibody activity and demonstrate the therapeutic potential of the Fc-free strategy that can be used advantageously to provide both immediate and long-term protection against SARS-CoV-2 and other viral infections.SARS-CoV-2 B.1.351 and B.1.167.2 viruses used in this study were obtained through the European Virus Archive Global (EVA-GLOBAL) project that has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 653316. SARS-CoV-2 B.1 (MAD6 isolate) was kindly provided by José M. Honrubia and Luis Enjuanes (CNB-CSIC, Madrid, Spain). The authors thank Centro de Investigación en Sanidad Animal (CISA)-Instituto Nacional de Investigaciones Agrarias (INIA-CSIC) (Valdeolmos, Madrid, Spain) for the BSL-3 facilities. Research in LA-V laboratory was funded by the BBVA Foundation (Ayudas Fundación BBVA a Equipos de Investigación Científica SARS-CoV-2 y COVID-19); the MCIN/AEI/10.13039/501100011033 (PID2020-117323RB-I00 and PDC2021-121711-I00), partially supported by the European Regional Development Fund (ERDF); the Carlos III Health Institute (ISCIII) (DTS20/00089), partially supported by the ERDF, the Spanish Association Against Cancer (AECC 19084); the CRIS Cancer Foundation (FCRIS-IFI-2018 and FCRIS-2021-0090), the Fundación Caixa-Health Research (HR21-00761 project IL7R_LungCan), and the Comunidad de Madrid (P2022/BMD-7225 NEXT_GEN_CART_MAD-CM). Work in the DS laboratory was funded by the CNIC; the European Union's Horizon 2020 research and innovation program under grant agreement ERC-2016-Consolidator Grant 725091; MCIN/AEI/10.13039/501100011033 (PID2019-108157RB); Comunidad de Madrid (B2017/BMD-3733 Immunothercan-CM); Atresmedia (Constantes y Vitales prize); Fondo Solidario Juntos (Banco Santander); and “La Caixa” Foundation (LCF/PR/HR20/00075). The CNIC was supported by the ISCIII, the MCIN and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (CEX2020-001041-S funded by MCIN/AEI/10.13039/501100011033). Research in RD laboratory was supported by the ISCIII (PI2100989) and CIBERINFEC; the European Commission Horizon 2020 Framework Programme (grant numbers 731868 project VIRUSCAN FETPROACT-2016, and 101046084 project EPIC-CROWN-2); and the Fundación Caixa-Health Research (grant number HR18-00469 project StopEbola). Research in CNB-CSIC laboratory was funded by Fondo Supera COVID-19 (Crue Universidades-Banco Santander) grant, CIBERINFEC, and Spanish Research Council (CSIC) grant 202120E079 (to J.G.-A.), CSIC grant 2020E84 (to M.E.), MCIN/AEI/10.13039/501100011033 (PID2020-114481RB-I00 to J.G-A. and M.E.), and by the European Commission-NextGenerationEU, through CSIC's Global Health Platform (PTI Salud Global) to J.G.-A. and M.E. Work in the CIB-CSIC laboratory was supported by MCIN/AEI/10.13039/501100011033 (PID2019-104544GB-I00 and 2023AEP105 to CA, and PID2020-113225GB-I00 to F.J.B.). Cryo-EM data were collected at the Maryland Center for Advanced Molecular Analyses which was supported by MPOWER (The University of Maryland Strategic Partnership). I.H.-M. receives the support of a fellowship from la Caixa Foundation (ID 100010434, fellowship code: LCF/BQ/IN17/11620074) and from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 71367. L.R.-P. was supported by a predoctoral fellowship from the Immunology Chair, Universidad Francisco de Vitoria/Merck.Peer reviewe