298 research outputs found
Low energy kinetic threshold in the growth of cubic boron nitride films
We report the growth of cubic boron nitride (cBN) films by magnetron sputtering on Si (100) substrates. The films are grown in the presence of negative substrate bias voltages and a nitrogen plasma produced by an electron cyclotron resonance source. We find evidence for a sharp low‐voltage threshold in the substrate bias (−105 V) beyond which the samples are predominantly cBN. The structural quality of the cBN films is optimized in a narrow range of voltages near this threshold. We discuss the important role of energetic ions in the formation of cBN in light of recent theoretical findings.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70509/2/APPLAB-64-14-1859-1.pd
Observation of near‐band‐gap luminescence from boron nitride films
We report results from cathodoluminescence spectroscopy of boron nitride films grown on Si(100) substrates by ion‐source‐assisted magnetron sputtering of a hexagonal BN target. Three main peaks are observed in the near‐band‐gap region for hexagonal boron nitride films at energies of 4.90, 5.31, and 5.50 eV. We also report deep‐level emission spectra of predominantly cubic boron nitride films which are correlated with sample growth conditions. In particular we show that the emission intensity, position, and linewidth are strongly dependent on the substrate bias voltage used during sample growth.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/69948/2/APPLAB-65-10-1251-1.pd
The origin of the legumes is a complex paleopolyploid phylogenomic tangle closely associated with the cretaceous-paleogene (K-Pg) mass extinction event
This is the final version. Available from Oxford University Press via the DOI in this record. The consequences of the Cretaceous-Paleogene (K-Pg) boundary (KPB) mass extinction for the evolution of plant diversity remain poorly understood, even though evolutionary turnover of plant lineages at the KPB is central to understanding assembly of the Cenozoic biota. The apparent concentration of whole genome duplication (WGD) events around the KPB may have played a role in survival and subsequent diversification of plant lineages. To gain new insights into the origins of Cenozoic biodiversity, we examine the origin and early evolution of the globally diverse legume family (Leguminosae or Fabaceae). Legumes are ecologically (co-)dominant across many vegetation types, and the fossil record suggests that they rose to such prominence after the KPB in parallel with several well-studied animal clades including Placentalia and Neoaves. Furthermore, multiple WGD events are hypothesized to have occurred early in legume evolution. Using a recently inferred phylogenomic framework, we investigate the placement of WGDs during early legume evolution using gene tree reconciliation methods, gene count data and phylogenetic supernetwork reconstruction. Using 20 fossil calibrations we estimate a revised timeline of legume evolution based on 36 nuclear genes selected as informative and evolving in an approximately clock-like fashion. To establish the timing of WGDs we also date duplication nodes in gene trees. Results suggest either a pan-legume WGD event on the stem lineage of the family, or an allopolyploid event involving (some of) the earliest lineages within the crown group, with additional nested WGDs subtending subfamilies Papilionoideae and Detarioideae. Gene tree reconciliation methods that do not account for allopolyploidy may be misleading in inferring an earlier WGD event at the time of divergence of the two parental lineages of the polyploid, suggesting that the allopolyploid scenario is more likely. We show that the crown age of the legumes dates to the Maastrichtian or early Paleocene and that, apart from the Detarioideae WGD, paleopolyploidy occurred close to the KPB. We conclude that the early evolution of the legumes followed a complex history, in which multiple auto- and/or allopolyploidy events coincided with rapid diversification and in association with the mass extinction event at the KPB, ultimately underpinning the evolutionary success of the Leguminosae in the Cenozoic.Swiss National Science FoundationUniversity of ZurichNatural Sciences and Engineering Research Council of CanadaNational Environment Research CouncilFonds de la Recherche Scientifique of Belgiu
The Nylon Scintillator Containment Vessels for the Borexino Solar Neutrino Experiment
Borexino is a solar neutrino experiment designed to observe the 0.86 MeV Be-7
neutrinos emitted in the pp cycle of the sun. Neutrinos will be detected by
their elastic scattering on electrons in 100 tons of liquid scintillator. The
neutrino event rate in the scintillator is expected to be low (~0.35 events per
day per ton), and the signals will be at energies below 1.5 MeV, where
background from natural radioactivity is prominent. Scintillation light
produced by the recoil electrons is observed by an array of 2240
photomultiplier tubes. Because of the intrinsic radioactive contaminants in
these PMTs, the liquid scintillator is shielded from them by a thick barrier of
buffer fluid. A spherical vessel made of thin nylon film contains the
scintillator, separating it from the surrounding buffer. The buffer region
itself is divided into two concentric shells by a second nylon vessel in order
to prevent inward diffusion of radon atoms. The radioactive background
requirements for Borexino are challenging to meet, especially for the
scintillator and these nylon vessels. Besides meeting requirements for low
radioactivity, the nylon vessels must also satisfy requirements for mechanical,
optical, and chemical properties. The present paper describes the research and
development, construction, and installation of the nylon vessels for the
Borexino experiment
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Measurement of geo-neutrinos from 1353 days of Borexino
We present a measurement of the geo--neutrino signal obtained from 1353 days
of data with the Borexino detector at Laboratori Nazionali del Gran Sasso in
Italy. With a fiducial exposure of (3.69 0.16) proton
year after all selection cuts and background subtraction, we detected
(14.3 4.4) geo-neutrino events assuming a fixed chondritic mass Th/U
ratio of 3.9. This corresponds to a geo-neutrino signal = (38.8
12.0) TNU with just a 6 probability for a null geo-neutrino
measurement. With U and Th left as free parameters in the fit, the relative
signals are = (10.6 12.7) TNU and =
(26.5 19.5) TNU. Borexino data alone are compatible with a mantle
geo--neutrino signal of (15.4 12.3) TNU, while a combined analysis with
the KamLAND data allows to extract a mantle signal of (14.1 8.1) TNU. Our
measurement of a reactor anti--neutrino signal =
84.5 TNU is in agreement with expectations in the presence of
neutrino oscillations.Comment: 9 pages, 6 figure
Borexino calibrations: Hardware, Methods, and Results
Borexino was the first experiment to detect solar neutrinos in real-time in
the sub-MeV region. In order to achieve high precision in the determination of
neutrino rates, the detector design includes an internal and an external
calibration system. This paper describes both calibration systems and the
calibration campaigns that were carried out in the period between 2008 and
2011. We discuss some of the results and show that the calibration procedures
preserved the radiopurity of the scintillator. The calibrations provided a
detailed understanding of the detector response and led to a significant
reduction of the systematic uncertainties in the Borexino measurements
The Borexino detector at the Laboratori Nazionali del Gran Sasso
Borexino, a large volume detector for low energy neutrino spectroscopy, is
currently running underground at the Laboratori Nazionali del Gran Sasso,
Italy. The main goal of the experiment is the real-time measurement of sub MeV
solar neutrinos, and particularly of the mono energetic (862 keV) Be7 electron
capture neutrinos, via neutrino-electron scattering in an ultra-pure liquid
scintillator. This paper is mostly devoted to the description of the detector
structure, the photomultipliers, the electronics, and the trigger and
calibration systems. The real performance of the detector, which always meets,
and sometimes exceeds, design expectations, is also shown. Some important
aspects of the Borexino project, i.e. the fluid handling plants, the
purification techniques and the filling procedures, are not covered in this
paper and are, or will be, published elsewhere (see Introduction and
Bibliography).Comment: 37 pages, 43 figures, to be submitted to NI
A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle
The TAR RNA of HIV was engineered as an siRNA delivery vehicle to develop a combinatorial therapeutic approach. The TAR backbone was found to be a versatile backbone for expressing siRNAs. Upon expression in human cells, pronounced and specific inhibition of reporter gene expression was observed with TARmiR. The resulting TARmiR construct retained its ability to bind Tat and mediate RNAi. TARmiR was able to inhibit HIV gene expression as a TAR decoy and by RNA interference when challenged with infectious proviral DNA. The implications of this dual function therapeutic would be discussed
The genomes of two key bumblebee species with primitive eusocial organization
Background: The shift from solitary to social behavior is one of the major evolutionary transitions. Primitively eusocial bumblebees are uniquely placed to illuminate the evolution of highly eusocial insect societies. Bumblebees are also invaluable natural and agricultural pollinators, and there is widespread concern over recent population declines in some species. High-quality genomic data will inform key aspects of bumblebee biology, including susceptibility to implicated population viability threats. Results: We report the high quality draft genome sequences of Bombus terrestris and Bombus impatiens, two ecologically dominant bumblebees and widely utilized study species. Comparing these new genomes to those of the highly eusocial honeybee Apis mellifera and other Hymenoptera, we identify deeply conserved similarities, as well as novelties key to the biology of these organisms. Some honeybee genome features thought to underpin advanced eusociality are also present in bumblebees, indicating an earlier evolution in the bee lineage. Xenobiotic detoxification and immune genes are similarly depauperate in bumblebees and honeybees, and multiple categories of genes linked to social organization, including development and behavior, show high conservation. Key differences identified include a bias in bumblebee chemoreception towards gustation from olfaction, and striking differences in microRNAs, potentially responsible for gene regulation underlying social and other traits. Conclusions: These two bumblebee genomes provide a foundation for post-genomic research on these key pollinators and insect societies. Overall, gene repertoires suggest that the route to advanced eusociality in bees was mediated by many small changes in many genes and processes, and not by notable expansion or depauperation
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