2,523 research outputs found
Holographic memory using beam steering
A method, apparatus, and system provide the ability for storing holograms at high speed. A single laser diode emits a collimated laser beam to both write to and read from a photorefractice crystal. One or more liquid crystal beam steering spatial light modulators (BSSLMs) steer a reference beam, split from the collimated laser beam, at high speed to the photorefractive crystal
Modeling and Simulation as Boundary Objects to Facilitate Interdisciplinary Research
Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/150614/1/sres2564.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150614/2/sres2564_am.pd
Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy
Development of single-molecule localization microscopy techniques has allowed nanometre scale localization accuracy inside cells, permitting the resolution of ultra-fine cell structure and the elucidation of crucial molecular mechanisms. Application of these methodologies to understanding processes underlying DNA replication and repair has been limited to defined in vitro biochemical analysis and prokaryotic cells. In order to expand these techniques to eukaryotic systems, we have further developed a photo-activated localization microscopy-based method to directly visualize DNA-associated proteins in unfixed eukaryotic cells. We demonstrate that motion blurring of fluorescence due to protein diffusivity can be used to selectively image the DNA-bound population of proteins. We designed and tested a simple methodology and show that it can be used to detect changes in DNA binding of a replicative helicase subunit, Mcm4, and the replication sliding clamp, PCNA, between different stages of the cell cycle and between distinct genetic backgrounds
Probing the Balance of AGN and Star-Forming Activity in the Local Universe with ChaMP
The combination of the SDSS and the Chandra Multiwavelength Project (ChaMP)
currently offers the largest and most homogeneously selected sample of nearby
galaxies for investigating the relation between X-ray nuclear emission, nebular
line-emission, black hole masses, and properties of the associated stellar
populations. We present here novel constraints that both X-ray luminosity Lx
and X-ray spectral energy distribution bring to the galaxy evolutionary
sequence H II -> Seyfert/Transition Object -> LINER -> Passive suggested by
optical data. In particular, we show that both Lx and Gamma, the slope of the
power-law that best fits the 0.5 - 8 keV spectra, are consistent with a clear
decline in the accretion power along the sequence, corresponding to a softening
of their spectra. This implies that, at z ~ 0, or at low luminosity AGN levels,
there is an anti-correlation between Gamma and L/Ledd, opposite to the trend
exhibited by high z AGN (quasars). The turning point in the Gamma -L/Ledd LLAGN
+ quasars relation occurs near Gamma ~ 1.5 and L/Ledd ~ 0.01. Interestingly,
this is identical to what stellar mass X-ray binaries exhibit, indicating that
we have probably found the first empirical evidence for an intrinsic switch in
the accretion mode, from advection-dominated flows to standard (disk/corona)
accretion modes in supermassive black hole accretors, similar to what has been
seen and proposed to happen in stellar mass black hole systems. The
anti-correlation we find between Gamma and L/Ledd may instead indicate that
stronger accretion correlates with greater absorption. Therefore the trend for
softer spectra toward more luminous, high redshift, and strongly accreting
AGN/quasars could simply be the result of strong selection biases reflected in
the dearth of type 2 quasar detections.Comment: 23 pages, 8 figures, 1 long (3 page) table, to appear in Ap
Wideband 67-116 GHz cryogenic receiver development for ALMA Band 2
The Atacama Large Millimeter/sub-millimeter Array (ALMA) is already
revolutionising our understanding of the Universe. However, ALMA is not yet
equipped with all of its originally planned receiver bands, which will allow it
to observe over the full range of frequencies from 35-950 GHz accessible
through the Earth's atmosphere. In particular Band 2 (67-90 GHz) has not yet
been approved for construction. Recent technological developments in cryogenic
monolithic microwave integrated circuit (MMIC) high electron mobility
transistor (HEMT) amplifier and orthomode transducer (OMT) design provide an
opportunity to extend the originally planned on-sky bandwidth, combining ALMA
Bands 2 and 3 into one receiver cartridge covering 67-116 GHz.
The IF band definition for the ALMA project took place two decades ago, when
8 GHz of on-sky bandwidth per polarisation channel was an ambitious goal. The
new receiver design we present here allows the opportunity to expand ALMA's
wideband capabilities, anticipating future upgrades across the entire
observatory. Expanding ALMA's instantaneous bandwidth is a high priority, and
provides a number of observational advantages, including lower noise in
continuum observations, the ability to probe larger portions of an astronomical
spectrum for, e.g., widely spaced molecular transitions, and the ability to
scan efficiently in frequency space to perform surveys where the redshift or
chemical complexity of the object is not known a priori. Wider IF bandwidth
also reduces uncertainties in calibration and continuum subtraction that might
otherwise compromise science objectives.
Here we provide an overview of the component development and overall design
for this wideband 67-116 GHz cryogenic receiver cartridge, designed to operate
from the Band 2 receiver cartridge slot in the current ALMA front end receiver
cryostat.Comment: 8 pages, proceedings from the 8th ESA Workshop on Millimetre-Wave
Technology and Applications
(https://atpi.eventsair.com/QuickEventWebsitePortal/millimetre-wave/mm-wave
Human urocortin II, a selective agonist for the type 2 corticotropinreleasing factor receptor, decreases feeding and drinking in the rat
ABSTRACT Corticotropin-releasing factor (CRF) has been hypothesized to modulate consummatory behavior through the Type 2 CRF (CRF 2 ) receptor. However, behavioral functions subserved by the CRF 2 receptor remain poorly understood. Recently, human urocortin II (hUcn II), a selective CRF 2 receptor agonist, was identified. To study the effects of this neuropeptide on ingestive behavior, we examined the effects of centrally infused hUcn II (i.c.v. 0, 0.01, 0.1, 1.0, 10.0 g) on the microstructure of nosepoke responding for food and water in nondeprived, male rats. Malaise-inducing properties of the peptide were monitored using conditioned taste aversion (CTA) testing. To identify potential sites of action, central induction of Fos protein expression was examined. hUcn II dose dependently reduced the quantity and duration of responding for food and water at doses lower (0.01-1.0 g) than that forming a CTA (10 g). Effects were most evident during hours 4 to 6 of the dark cycle. Meal pattern analysis showed that hUcn II potently (0.1 g) increased the satiating value of food. Rats ate and drank smaller and shorter meals without changing meal frequency. Rats also ate more slowly. hUcn II induced Fos in regions involved in visceral sensory processing and autonomic/neuroendocrine regulation and resembling those activated by appetite suppressants. hUcn II is a promising neuropeptide for investigating the role of the CRF 2 receptor in ingestive behavior. Corticotropin-releasing factor (CRF) is hypothesized to mediate behavioral, autonomic, endocrine, and immunological responses to stres
Improving the delivery and efficiency of fungus-impregnated cloths for control of adult Aedes aegypti using a synthetic attractive lure
Abstract Background Entomopathogenic fungi are highly promising agents for controlling Aedes aegypti mosquitoes. Deploying fungus-impregnated black cloths in PET traps efficiently reduced Ae. aegypti female survival rates under intra-domicile conditions. With the aim of further increasing the effectiveness of the traps, the addition of attractive lures to fungus-impregnated traps was evaluated. Methods Black cloths were suspended inside 2 l plastic bottles called “PET traps”. These traps were placed in rooms simulating human residences. The first experiments evaluated the attraction of mosquitoes to PET traps with black cloths covered in adhesive film with and without synthetic lures (AtrAedes™). Traps were left in the test rooms for either 24 or 48 h. The attractiveness of the lures over time was also evaluated. The efficiency of PET traps with fungus-impregnated black cloths associated with lures was compared to that of traps without lures. Results The highest percentage of captured mosquitoes (31 and 66%) were observed in PET traps with black cloths covered in adhesive film + attractive lure maintained in test rooms for 24 h and 48 h, respectively. Black cloths covered in adhesive film captured 17 or 36% of the mosquitoes at 24 h and 48 h, respectively. The attractiveness of the lures fell gradually over time, capturing 37% after 5 days on the bench and 22% of the mosquitoes after 30 days exposure to ambient conditions. Associating attractive synthetic lures with black cloths impregnated with M. anisopliae placed in test rooms for 120 h reduced mean survival to 32%, whilst black cloths impregnated with M. anisopliae without lures resulted in a 48% survival rate. Using Beauveria bassiana in the traps resulted in a 52% reduction in mosquito survival, whilst combining Beauveria and AtrAedes resulted in a 36% survival rate. PET traps impregnated with fungus + AtrAedes resulted in similar reductions in survival when left in the rooms for 24, 48, 72 or 120 h. Conclusions AtrAedes increased attractiveness of PET traps with black cloths under intra-domicile conditions and when associated with M. anisopliae or B. bassiana, significantly reduced Aedes survival. This strategy will reduce the number of PET traps necessary per household
First narrow-band search for continuous gravitational waves from known pulsars in advanced detector data
Spinning neutron stars asymmetric with respect to their rotation axis are potential sources of
continuous gravitational waves for ground-based interferometric detectors. In the case of known pulsars a
fully coherent search, based on matched filtering, which uses the position and rotational parameters
obtained from electromagnetic observations, can be carried out. Matched filtering maximizes the signalto-
noise (SNR) ratio, but a large sensitivity loss is expected in case of even a very small mismatch
between the assumed and the true signal parameters. For this reason, narrow-band analysis methods have
been developed, allowing a fully coherent search for gravitational waves from known pulsars over a
fraction of a hertz and several spin-down values. In this paper we describe a narrow-band search of
11 pulsars using data from Advanced LIGO’s first observing run. Although we have found several initial
outliers, further studies show no significant evidence for the presence of a gravitational wave signal.
Finally, we have placed upper limits on the signal strain amplitude lower than the spin-down limit for 5 of
the 11 targets over the bands searched; in the case of J1813-1749 the spin-down limit has been beaten for
the first time. For an additional 3 targets, the median upper limit across the search bands is below the
spin-down limit. This is the most sensitive narrow-band search for continuous gravitational waves carried
out so far
Compressed representation of a partially defined integer function over multiple arguments
In OLAP (OnLine Analitical Processing) data are analysed in an n-dimensional cube. The cube may be represented as a partially defined function over n arguments. Considering that often the function is not defined everywhere, we ask: is there a known way of representing the function or the points in which it is defined, in a more compact manner than the trivial one
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