Construcción de un servidor geográfico web, como herramienta de visualización de un observatorio urbano, utilizando tecnologías open source

El presente trabajo de Tesis tiene como finalidad mostrar los resultados obtenidos al aplicar tecnologías Open Source como MapServer en la creación de un Servidor geográfico web para el Observatorio Metropolitano de Puebla como herramienta de visualización de resultados de los indicadores urbanos implementados por la Red Nacional de Observatorios Urbanos constituida por la SEDESOL y, que a su vez, dan respuesta a los objetivos del desarrollo del Milenio, establecidos por la ONU

Gestión Ambiental en los Campos de Golf de Los Cabos, B.C.S.

La gestión ambiental es un instrumento empresarial de reciente aplicación que enfatiza en la minimización de los impacto ambientales e incentiva la producción limpia a través de la mejora de los procesos y la integración de tecnologías amigables con la naturaleza. Este artículo presenta los resultados sobre la gestión ambiental en los campos de golf de Los Cabos, Baja California Sur (BCS) desde una perspectiva empresarial. Su relevancia radica en que esta región cuenta con ambientes naturales únicos cuyos recursos hídricos son limitados, por lo que una eficiente gestión de éstos podría contribuir a la sustentabilidad del destino turístico. Se trata de una investigación que respondió a argumentos y criterios de origen cualitativo, se recopiló información de siete campos de golf a través de instrumentos de evaluación, entrevistas a profundidad y observación directa de tres categorías: a) monitoreo y control operacional, b) gestión ambiental organizacional y, c) sistemas de gestión ambiental (SGA) y su certificación. Los principales resultados muestran fortalezas respecto a la conservación de los suelos, el manejo de residuos y la protección de la biodiversidad, no obstante existen debilidades en la gestión de los recursos hídricos, la energía, las emisiones atmosféricas y el ruido. La gestión ambiental resulta ser apenas un comienzo voluntario entre los campos de golf de Los Cabos, lo que requiere de un mayor esfuerzo por parte de los empresarios como una posibilidad sustentable para el sector

A monoclonal antibody-based immunoassay to measure the antibody response against the repeat region of the circumsporozoite protein of Plasmodium falciparum

Background: The malaria vaccine candidate RTS, S/AS01 (GSK Vaccines) induces high IgG concentration against the circumsporozoite protein (CSP) of Plasmodium falciparum. In human vaccine recipients circulating anti-CSP antibody concentrations are associated with protection against infection but appear not to be the correlate of protection. However, in a humanized mouse model of malaria infection prophylactic administration of a human monoclonal antibody (MAL1C), derived from a RTS, S/AS01-immunized volunteer, directed against the CSP repeat region, conveyed full protection in a dose-dependent manner suggesting that antibodies alone are able to prevent P. falciparum infection when present in sufficiently high concentrations. A competition ELISA was developed to measure the presence of MAL1C-like antibodies in polyclonal sera from RTS, S/AS01 vaccine recipients and study their possible contribution to protection against infection. Results: MAL1C-like antibodies present in polyclonal vaccine-induced sera were evaluated for their ability to compete with biotinylated monoclonal antibody MAL1C for binding sites on the capture antigen consisting of the recombinant protein encompassing 32 NANP repeats of CSP (R32LR). Serum samples were taken at different time points from participants in two RTS, S/AS01 vaccine studies (NCT01366534 and NCT01857869). Vaccine-induced protection status of the study participants was determined based on the outcome of experimental challenge with infected mosquito bites after vaccination. Optimal conditions were established to reliably detect MAL1C-like antibodies in polyclonal sera. Polyclonal anti-CSP antibodies and MAL1C-like antibody content were measured in 276 serum samples from RTS, S/AS01 vaccine recipients using the standard ELISA and MAL-1C competition ELISA, respectively. A strong correlation was observed between the results from these assays. However, no correlation was found between the results of either assay and protection against infection. Conclusions: The competition ELISA to measure MAL1C-like antibodies in polyclonal sera from RTS, S/AS01 vaccine recipients was robust and reliable but did not reveal the elusive correlate of protection

Introducing EbolaCheck: potential for point-of-need infectious disease diagnosis

The 2013–2015 Ebolavirus disease humanitarian crisis has spurred the development of laboratory-free, point-of-care nucleic acid testing solutions. EbolaCheck is an international consortium of public health, academic and biotechnology industry stakeholders aiming to deliver clinical molecular diagnostic standard-of-care testing suitable for the West African milieu within 12 months. In this article, the current status of the EbolaCheck platform is discussed in the context of the current regulatory framework. Presented here are future goals to achieve differential diagnosis of hemorrhagic fever disease from <5-μl of whole blood samples or mucosal biofluids, in a single tube process, under 40 min and with minimal operator training requirements

A tutela de urgência sob a ótica da arbitragem internacional: o poder do árbitro para proferir medidas acautelatórias.

TCC(graduação) - Universidade Federal de Santa Catarina. Centro de Ciências Jurídicas. Direito.A presente monografia trata do cabimento das tutelas de urgência no curso do procedimento arbitral internacional. O objetivo proposto é confirmar a hipótese provisória de que o árbitro ou tribunal arbitral tem o poder para conceder tutelas de urgência, uma vez que a competência para conceder medidas acautelatórias é ampla para a concessão de quaisquer medidas e que sejam preenchidos os requisitos para sua concessão. No referente à metodologia científica foi utilizado o método indutivo como método de procedimento monográfico e como técnica de pesquisa, foi utilizada a bibliográfica e documental. As fontes de pesquisa utilizadas foram: a legislação em matéria de arbitragem (nacional e estrangeira), a normativa internacional, sentenças arbitrais, doutrina e finalmente, jurisprudência. Também foram incluídos os regulamentos de câmaras de arbitragem. Os resultados revelam que a existência do poder arbitral não deve presumir-se e ao contrário, o mesmo encontra fundamento no consentimento expresso ou implícito das partes. O tema ganhou ainda maior atenção após a recente incorporação em vários regulamentos de arbitragem do chamado “árbitro emergencial”. Justamente, espera-se que o uso e importância das tutelas de urgência aumentem significativamente com a recente adoção pelas principais instituições de arbitragem do chamado “árbitro de emergência”. Este trabalho contribui para a compreensão de questões relativas ao poder arbitral, em matéria de tutelas de urgência no curso do processo arbitral internacional, seu fundamento e seus limites

Un nuevo testimonio documental sobre el intercambio cultural en el siglo XVI: el libro de tributos de San Pablo Teocaltitlan, 1574

Artículo de investigación científica sobre el registro de un padrón de tributarios de una parcialidad de san Pablo Teopan en la segunda mitad del siglo XV

The current landscape of nucleic acid tests for filovirus detection.

Nucleic acid testing (NAT) for pathogenic filoviruses plays a key role in surveillance and to control the spread of infection. As they share clinical features with other pathogens, the initial spread of these viruses can be misdiagnosed. Tests that can identify a pathogen in the initial stages of infection are essential to control outbreaks. Since the Ebola virus disease (EVD) outbreak in 2014-2016 several tests have been developed that are faster than previous tests and more suited for field use. Furthermore, the ability to test for a range of pathogens simultaneously has been expanded to improve clinical pathway management of febrile syndromes. This review provides an overview of these novel diagnostic tests

The Influence of Genotype and Environment on Small RNA Profiles in Grapevine Berry

Understanding the molecular mechanisms involved in the interaction between the genetic composition and the environment is crucial for modern viticulture. We approached this issue by focusing on the small RNA transcriptome in grapevine berries of the two varieties Cabernet Sauvignon and Sangiovese, growing in adjacent vineyards in three different environments. Four different developmental stages were studied and a total of 48 libraries of small RNAs were produced and sequenced. Using a proximity-based pipeline, we determined the general landscape of small RNAs accumulation in grapevine berries. We also investigated the presence of known and novel miRNAs and analyzed their accumulation profile. The results showed that the distribution of small RNA-producing loci is variable between the two cultivars, and that the level of variation depends on the vineyard. Differently, the profile of miRNA accumulation mainly depends on the developmental stage. The vineyard in Riccione maximizes the differences between the varieties, promoting the production of more than 1000 specific small RNA loci and modulating their expression depending on the cultivar and the maturation stage. In total, 89 known vvi-miRNAs and 33 novel vvi-miRNA candidates were identified in our samples, many of them showing the accumulation profile modulated by at least one of the factors studied. The in silico prediction of miRNA targets suggests their involvement in berry development and in secondary metabolites accumulation such as anthocyanins and polyphenols