p21-activated kinase (PAK) regulates cytoskeletal reorganization and directional migration in human neutrophils

Neutrophils serve as a first line of defense in innate immunity owing in part to their ability to rapidly migrate towards chemotactic factors derived from invading pathogens. As a migratory function, neutrophil chemotaxis is regulated by the Rho family of small GTPases. However, the mechanisms by which Rho GTPases orchestrate cytoskeletal dynamics in migrating neutrophils remain ill-defined. In this study, we characterized the role of p21-activated kinase (PAK) downstream of Rho GTPases in cytoskeletal remodeling and chemotactic processes of human neutrophils. We found that PAK activation occurred upon stimulation of neutrophils with f-Met-Leu-Phe (fMLP), and PAK accumulated at the actin-rich leading edge of stimulated neutrophils, suggesting a role for PAK in Rac-dependent actin remodeling. Treatment with the pharmacological PAK inhibitor, PF3758309, abrogated the integrity of RhoA-mediated actomyosin contractility and surface adhesion. Moreover, inhibition of PAK activity impaired neutrophil morphological polarization and directional migration under a gradient of fMLP, and was associated with dysregulated Ca2+ signaling. These results suggest that PAK serves as an important effector of Rho-family GTPases in neutrophil cytoskeletal reorganization, and plays a key role in driving efficient directional migration of human neutrophils

Data Analysis Challenges for the Einstein Telescope

The Einstein Telescope is a proposed third generation gravitational wave detector that will operate in the region of 1 Hz to a few kHz. As well as the inspiral of compact binaries composed of neutron stars or black holes, the lower frequency cut-off of the detector will open the window to a number of new sources. These will include the end stage of inspirals, plus merger and ringdown of intermediate mass black holes, where the masses of the component bodies are on the order of a few hundred solar masses. There is also the possibility of observing intermediate mass ratio inspirals, where a stellar mass compact object inspirals into a black hole which is a few hundred to a few thousand times more massive. In this article, we investigate some of the data analysis challenges for the Einstein Telescope such as the effects of increased source number, the need for more accurate waveform models and the some of the computational issues that a data analysis strategy might face.Comment: 18 pages, Invited review for Einstein Telescope special edition of GR

The Challenges in Gravitational Wave Astronomy for Space-Based Detectors

The Gravitational Wave (GW) universe contains a wealth of sources which, with the proper treatment, will open up the universe as never before. By observing massive black hole binaries to high redshifts, we should begin to explore the formation process of seed black holes and track galactic evolution to the present day. Observations of extreme mass ratio inspirals will allow us to explore galactic centers in the local universe, as well as providing tests of General Relativity and constraining the value of Hubble's constant. The detection of compact binaries in our own galaxy may allow us to model stellar evolution in the Milky Way. Finally, the detection of cosmic (super)strings and a stochastic background would help us to constrain cosmological models. However, all of this depends on our ability to not only resolve sources and carry out parameter estimation, but also on our ability to define an optimal data analysis strategy. In this presentation, I will examine the challenges that lie ahead in GW astronomy for the ESA L3 Cosmic Vision mission, eLISA.Comment: 12 pages. Plenary presentation to appear in the Proceedings of the Sant Cugat Forum on Astrophysics, Sant Cugat, April 22-25, 201

Community structures of actively growing bacteria shift along a north-south transect in the western North Pacific

Bacterial community structures and their activities in the ocean are tightly coupled with organic matter fluxes and thus control ocean biogeochemical cycles. Bromodeoxyuridine (BrdU), halogenated nucleoside and thymidine analogue, has been recently used to monitor actively growing bacteria (AGB) in natural environments. We labelled DNA of proliferating cells in seawater bacterial assemblages with BrdU and determined community structures of the bacteria that were possible key species in mediating biochemical reactions in the ocean. Surface seawater samples were collected along a north-south transect in the North Pacific in October 2003 and subjected to BrdU magnetic beads immunocapture and PCR-DGGE (BUMP-DGGE) analysis. Change of BrdU-incorporated community structures reflected the change of water masses along a north-south transect from subarctic to subtropical gyres in the North Pacific. We identified 25 bands referred to AGB as BrdU-incorporated phylotypes, belonging to Alphaproteobacteria (5 bands), Betaproteobacteria (1 band), Gammaproteobacteria (4 bands), Cytophaga-Flavobacterium-Bacteroides (CFB) group bacteria (5 bands), Gram-positive bacteria (6 bands), and Cyanobacteria (4 bands). BrdU-incorporated phylotypes belonging to Vibrionales, Alteromonadales and Gram-positive bacteria appeared only at sampling stations in a subtropical gyre, while those belonging to Roseobacter-related bacteria and CFB group bacteria appeared at the stations in both subarctic and subtropical gyres. Our result revealed phylogenetic affiliation of AGB and their dynamic change along with north-south environmental gradients in open oceans. Different species of AGB utilize different amount and kinds of substrates, which can affect the change of organic matter fluxes along transect

Globalization intentions in tension: The case of Singapore

10.1177/0020872810371202International Social Work535671-68

Supervisors' Perceptions of the Performance of Cooperative Education Employees Working in Federal Agencies

Through cooperative education programs, many public agencies employ college students part time or intermittently and groom them for future full-time employment The combination of winnowing and nurturing that occurs in these programs is believed to produce higher performing employees This study tests this hypothesis by comparing Federal supervisors' perceptions of the performance of co-op employees with those recruited from other sources Data come from the 1992 Merit Principles Survey, US Merit Systems Protection Board The results indicate that co-op employees perform at high levels, but they do not outperform other employees as a whole Next, we compare the performance ratings of Federal workers from seven other recruitment sources to see if any source is superior Some interesting findings emerge Of course, performance ratings are an incomplete indicator of an employee's value to the organization These ratings merely reflect supervisors' perceptions, and while high performance is important, agencies may wish to promote other goals in their recruitment and retention efforts such as workforce diversityYeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline

Measurement of the polarisation of W bosons produced with large transverse momentum in pp collisions at sqrt(s) = 7 TeV with the ATLAS experiment

This paper describes an analysis of the angular distribution of W->enu and W->munu decays, using data from pp collisions at sqrt(s) = 7 TeV recorded with the ATLAS detector at the LHC in 2010, corresponding to an integrated luminosity of about 35 pb^-1. Using the decay lepton transverse momentum and the missing transverse energy, the W decay angular distribution projected onto the transverse plane is obtained and analysed in terms of helicity fractions f0, fL and fR over two ranges of W transverse momentum (ptw): 35 < ptw < 50 GeV and ptw > 50 GeV. Good agreement is found with theoretical predictions. For ptw > 50 GeV, the values of f0 and fL-fR, averaged over charge and lepton flavour, are measured to be : f0 = 0.127 +/- 0.030 +/- 0.108 and fL-fR = 0.252 +/- 0.017 +/- 0.030, where the first uncertainties are statistical, and the second include all systematic effects.Comment: 19 pages plus author list (34 pages total), 9 figures, 11 tables, revised author list, matches European Journal of Physics C versio

Observation of a new chi_b state in radiative transitions to Upsilon(1S) and Upsilon(2S) at ATLAS

The chi_b(nP) quarkonium states are produced in proton-proton collisions at the Large Hadron Collider (LHC) at sqrt(s) = 7 TeV and recorded by the ATLAS detector. Using a data sample corresponding to an integrated luminosity of 4.4 fb^-1, these states are reconstructed through their radiative decays to Upsilon(1S,2S) with Upsilon->mu+mu-. In addition to the mass peaks corresponding to the decay modes chi_b(1P,2P)->Upsilon(1S)gamma, a new structure centered at a mass of 10.530+/-0.005 (stat.)+/-0.009 (syst.) GeV is also observed, in both the Upsilon(1S)gamma and Upsilon(2S)gamma decay modes. This is interpreted as the chi_b(3P) system.Comment: 5 pages plus author list (18 pages total), 2 figures, 1 table, corrected author list, matches final version in Physical Review Letter

Search for displaced vertices arising from decays of new heavy particles in 7 TeV pp collisions at ATLAS

We present the results of a search for new, heavy particles that decay at a significant distance from their production point into a final state containing charged hadrons in association with a high-momentum muon. The search is conducted in a pp-collision data sample with a center-of-mass energy of 7 TeV and an integrated luminosity of 33 pb^-1 collected in 2010 by the ATLAS detector operating at the Large Hadron Collider. Production of such particles is expected in various scenarios of physics beyond the standard model. We observe no signal and place limits on the production cross-section of supersymmetric particles in an R-parity-violating scenario as a function of the neutralino lifetime. Limits are presented for different squark and neutralino masses, enabling extension of the limits to a variety of other models.Comment: 8 pages plus author list (20 pages total), 8 figures, 1 table, final version to appear in Physics Letters