An HST optical-to-near-IR transmission spectrum of the hot Jupiter WASP-19b: detection of atmospheric water and likely absence of TiO

Copyright © 2013 Royal Astronomical SocietyWe measure the transmission spectrum of WASP-19b from three transits using low-resolution optical spectroscopy from the Hubble Space Telescope (HST) Space Telescope Imaging Spectrograph (STIS). The STIS spectra cover a wavelength range of 0.29–1.03 μm, with resolving power R = 500. The optical data are combined with archival near-infrared data from the HST Wide Field Camera 3 (WFC3) G141 grism, covering the wavelength range from 1.087 to 1.687 μm, with resolving power R = 130. We reach signal-to-noise levels between 3000 and 11 000 in 0.1 μm bins when measuring the transmission spectra from 0.53–1.687 μm. WASP-19 is known to be a very active star, with the optical stellar flux varying by a few per cent over time. We correct the transit light curves for the effects of stellar activity using ground-based activity monitoring with the Cerro Tololo Inter-American Observatory. While we were not able to construct a transmission spectrum using the blue optical data because of the presence of large occulted starspots, we were able to use the spot crossings to help constrain the mean stellar spot temperature. To search for predicted features in the hot-Jupiter atmosphere, in addition to the transmission spectrum we also define spectral indices for differential radius (ΔRP/R⋆) measurements to specifically search for the presence of TiO and alkali line features. Our measurements rule out TiO features predicted for a planet of WASP-19b's equilibrium temperature (2050 K) in the transmission spectrum at the 2.7–2.9σ confidence level, depending on atmospheric model formalism. The WFC3 transmission spectrum shows strong absorption features due to the presence of H2O, which is detected at the 4σ confidence level between 1.1 and 1.4 μm. The transmission spectra results indicate that WASP-19b is a planet with no or low levels of TiO and without a high C/O ratio. The lack of observable TiO features are possibly due to rainout, breakdown from stellar activity or the presence of other absorbers in the optical

Improved Adenovirus Vectors for Infection of Cardiovascular Tissues

To identify improved adenovirus vectors for cardiovascular gene therapy, a library of adenovirus vectors based on adenovirus serotype 5 (Ad5) but carrying fiber molecules of other human serotypes, was generated. This library was tested for efficiency of infection of human primary vascular endothelial cells (ECs) and smooth muscle cells (SMCs). Based on luciferase, LacZ, or green fluorescent protein (GFP) marker gene expression, several fiber chimeric vectors were identified that displayed improved infection of these cell types. One of the viruses that performed particularly well is an Ad5 carrying the fiber of Ad16 (Ad5.Fib16), a subgroup B virus. This virus showed, on average, 8- and 64-fold-increased luciferase activities on umbilical vein ECs and SMCs, respectively, compared to the parent vector. GFP and lacZ markers showed that approximately 3-fold (ECs) and 10-fold (SMCs) more cells were transduced. Experiments performed with both cultured SMCs and organ cultures derived from different vascular origins (saphenous vein, iliac artery, left interior mammary artery, and aorta) and from different species demonstrated that Ad5.Fib16 consistently displays improved infection in primates (humans and rhesus monkeys). SMCs of the same vessels of rodents and pigs were less infectable with Ad5.Fib16 than with Ad5. This suggests that either the receptor for human Ad16 is not conserved between different species or that differences in the expression levels of the putative receptor exist. In conclusion, our results show that an Ad5-based virus carrying the fiber of Ad16 is a potent vector for the transduction of primate cardiovascular cells and tissues