Peptide immobilisation on porous silicon surface for metal ions detection

In this work, a Glycyl-Histidyl-Glycyl-Histidine (GlyHisGlyHis) peptide is covalently anchored to the porous silicon PSi surface using a multi-step reaction scheme compatible with the mild conditions required for preserving the probe activity. In a first step, alkene precursors are grafted onto the hydrogenated PSi surface using the hydrosilylation route, allowing for the formation of a carboxyl-terminated monolayer which is activated by reaction with N-hydroxysuccinimide in the presence of a peptide-coupling carbodiimide N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide and subsequently reacted with the amino linker of the peptide to form a covalent amide bond. Infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy are used to investigate the different steps of functionalization

Histoire médicale générale et particulière des maladies épidémiques, contagieuses et épizootiques, qui ont régné en Europe depuis les temps les plus reculés jusqu'a nos jours /

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Histoire médicale générale et particulière des maladies épidémiques : contagieuses et épizootiques : qui ont régné en Europe depuis les temps les plus reculés jusqu'a nos jours /

Library has v. 1-2.Bibliography: v. 4, p. 346-375.Mode of access: Internet.former call number: Ozana