8 research outputs found
Towards a Clinically Relevant Lentiviral Transduction Protocol for Primary Human CD34+ Hematopoietic Stem/Progenitor Cells
- Author
- A Aiuti
- Allison Arndt
- AR Schroder
- C Baum
- C Donaldson
- CE Dunbar
- CI Lee
- DG Miller
- E Montini
- E Prus
- E Vigna
- F Di Nunzio
- F Mazurier
- FD Robart
- FR Santoni de Sio
- G Mostoslavsky
- G Pawelec
- H Glimm
- H Mikkers
- IR Lemischka
- J Barquinero
- J Wilpshaar
- JL Spees
- JS Dando
- K Pike-Overzet
- L Ailles
- L Armstrong
- L Gammaitoni
- L Tuschong
- M Bokhoven
- M Cavazzana-Calvo
- M De Palma
- M Tesio
- Maureen Boyd
- MG Ott
- MH Wu
- Michelle Millington
- MP McCormack
- MS Even
- N Rosenthal
- O Christ
- O Cohen-Haguenauer
- O Tiscornia G Singer
- OS Kustikova
- P Kurre
- R Zufferey
- RE Champlin
- RE Sutto
- RW Storms
- S Barrette
- S Charrier
- SF Williams
- SH Bokman
- SK Nayak
- SO Peter
- Sylvie Shen
- T Dull
- Tanya Applegate
- TY Roe
- U Modlich
- VN Kim
- W Piacibello
- William Giannobile
- WW Ward
- X Li
- X Wu
- Y Cui
- Z Li
- Publication venue
- Public Library of Science
- Publication date
- 01/01/2009
- Field of study
Background: Hematopoietic stem cells (HSC), in particular mobilized peripheral blood stem cells, represent an attractive target for cell and gene therapy. Efficient gene delivery into these target cells without compromising self-renewal and multipotency is crucial for the success of gene therapy. We investigated factors involved in the ex vivo transduction of CD34 + HSCs in order to develop a clinically relevant transduction protocol for gene delivery. Specifically sought was a protocol that allows for efficient transduction with minimal ex vivo manipulation without serum or other reagents of animal origin. Methodology/Principal Findings: Using commercially available G-CSF mobilized peripheral blood (PB) CD34 + cells as the most clinically relevant target, we systematically examined factors including the use of serum, cytokine combinations, prestimulation time, multiplicity of infection (MOI), transduction duration and the use of spinoculation and/or retronectin. A self-inactivating lentiviral vector (SIN-LV) carrying enhanced green fluorescent protein (GFP) was used as the gene delivery vehicle. HSCs were monitored for transduction efficiency, surface marker expression and cellular function. We were able to demonstrate that efficient gene transduction can be achieved with minimal ex vivo manipulation while maintaining the cellular function of transduced HSCs without serum or other reagents of animal origin. Conclusions/Significance: This study helps to better define factors relevant towards developing a standard clinical protocol for the delivery of SIN-LV into CD34 + cells
Peripheral Delivery of a CNS Targeted, Metalo-Protease Reduces Aβ Toxicity in a Mouse Model of Alzheimer's Disease
- Author
- A Pfeifer
- AC Cuello
- AC Cuello
- Anthony Adame
- Ashley Bush
- B Spencer
- B Spencer
- BJ Spencer
- Brian Spencer
- CS Hong
- DM Walsh
- DN Arvanitis
- E Masliah
- E Pham
- E Rockenstein
- E Rockenstein
- E Rockenstein
- Edward Rockenstein
- Eliezer Masliah
- F Huang
- FM LaFerla
- G Tiscornia
- GK Gouras
- GM Shankar
- H Kanemitsu
- HF Poon
- Inder M. Verma
- J Ray
- J Tian
- JB Rose
- JC Connelly
- JW Ashford
- K Iijima-Ando
- L Mucke
- L Yang
- M Yamamoto
- MA Leissring
- MA Shipp
- MH Mohajeri
- ML Hemming
- N Iwata
- N Iwata
- N Iwata
- O Singer
- O Wirths
- PJ Crouch
- PN Lacor
- PR Hof
- R Terry
- RA Marr
- RA Marr
- Rewati Potkar
- Robert A. Marr
- Ryan Gindi
- S Howell
- S Li
- Sarah Michael
- SM Huang
- T Saito
- T Tomiyama
- V Betts
- WJ Meilandt
- WM Pardridge
- Y Liu
- YS Eisele
- Publication venue
- Public Library of Science
- Publication date
- 01/01/2011
- Field of study
Alzheimer's disease (AD), an incurable, progressive neurodegenerative disorder, is the most common form of dementia. Therapeutic options have been elusive due to the inability to deliver proteins across the blood-brain barrier (BBB). In order to improve the therapeutic potential for AD, we utilized a promising new approach for delivery of proteins across the BBB. We generated a lentivirus vector expressing the amyloid β-degrading enzyme, neprilysin, fused to the ApoB transport domain and delivered this by intra-peritoneal injection to amyloid protein precursor (APP) transgenic model of AD. Treated mice had reduced levels of Aβ, reduced plaques and increased synaptic density in the CNS. Furthermore, mice treated with the neprilysin targeting the CNS had a reversal of memory deficits. Thus, the addition of the ApoB transport domain to the secreted neprilysin generated a non-invasive therapeutic approach that may be a potential treatment in patients with AD
Late postprandial pancreatic secretion periods in conscious dogs, Effect of vagotomy
- Author
- Publication venue
- 'Informa UK Limited'
- Publication date
- Field of study
Lentiviral Transgenesis
- Author
- A Follenzi
- A Hofmann
- A Hofmann
- A Pfeifer
- A Pfeifer
- A Pfeifer
- A Pfeifer
- A Reynolds
- AJ Bridge
- AW Chan
- BB Scott
- BS Heale
- C Lois
- CB Whitelaw
- CD Novina
- D Boden
- DA Rubinson
- DS Schwarz
- G Tiscornia
- GJ Hannon
- H Miyoshi
- JW Gordon
- L Naldini
- MA Robbins
- MJ McGrew
- NS Lee
- O Singer
- R Jaenisch
- R Jaenisch
- R Jaenisch
- SP Goff
- T Dull
- TR Brummelkamp
- V Hornung
- Y Furumoto
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Nestin negatively regulates postsynaptic differentiation of the neuromuscular synapse
- Author
- A Asada
- AK Fu
- AV Gilyarov
- Bertha Dominguez
- BT Helfand
- C Weston
- CL Hyder
- CM Sahlgren
- CM Sahlgren
- CM Sahlgren
- CW Lee
- E Perlson
- F Chen
- Fred de Winter
- G Tiscornia
- GN Patrick
- GN Patrick
- H Flanagan-Steet
- H Herrmann
- H Kang
- H Wu
- HM Pallari
- I Izawa
- J Linnoila
- J Nazarian
- Jiefei Yang
- John E Eriksson
- JR Sanes
- K Michalczyk
- Kuo-Fen Lee
- L Carlsson
- L Shi
- M Hamann
- MB Omary
- MC An
- NO Ku
- NO Ku
- O Singer
- R Madhavan
- RJ Bloch
- RJ Bloch
- RK Sihag
- S Hombach-Klonisch
- S Kesavapany
- S Kim
- S Lin
- S Vaittinen
- S Wu
- Thomas W Gould
- W Lin
- X Yang
- X Yang
- XP Dong
- ZG Luo
- ZG Luo
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Somatic Genetics Empowers the Mouse for Modeling and Interrogating Developmental and Disease Processes
- Author
- A Bric
- A Keravala
- AD Miller
- AG Uren
- AJ Dupuy
- AJ Dupuy
- AJ Dupuy
- AJ Dupuy
- AM Bender
- AM Geurts
- AR Muotri
- B Vogelstein
- C Tros de Ilarduya
- CE Meacham
- D Hanahan
- D Nguyen
- D Xu
- DW Parsons
- E Heard
- EC Holland
- EC Holland
- F Barnabe-Heider
- F Doetsch
- G Liu
- G Luo
- G Tiscornia
- GJ Murphy
- Gregory A. Cox
- GS Vassiliou
- H Mikkers
- H Nakanishi
- H Zong
- HH Fiebig
- IH Park
- J Cadinanos
- J Hanna
- J Jonkers
- J Kool
- J Yu
- JB Bell
- JI Johnson
- K Horie
- K Rajewsky
- K Takahashi
- K Takahashi
- K Yusa
- KA Choate
- KM Kahlig
- L Cooley
- L Naldini
- L Naldini
- L Zender
- LD Shultz
- LD Wood
- LE Woodard
- LM Mir
- LS Collier
- LS Collier
- M Kumar
- M Meyerson
- M Wernig
- MA Brehm
- MJ Federspiel
- N Kleckner
- N Legrand
- NE Sharpless
- NG Coufal
- O Tsuji
- Q Liang
- R Beroukhim
- R Rad
- RA Pagliarini
- RJ Leary
- RM White
- S Ding
- S Jones
- S Jones
- S Orsulic
- S Wu
- SE Fischer
- Sean F. Landrette
- SK Saridey
- SM Wiesner
- SR Yant
- T Lee
- T Marumoto
- T Singer
- T Sjoblom
- T Xu
- TA Elick
- Tian Xu
- TK Starr
- VW Keng
- VW Keng
- W Wang
- W Wang
- X Huang
- Y Nakazawa
- Z Alipio
- Z Ivics
- Publication venue
- 'Public Library of Science (PLoS)'
- Publication date
- Field of study
Spezielle Pathologie des Gesichtsfeldes
- Author
- A Antonelli
- A Aracri
- A Arsumaroff
- A Barkan
- A Baserga
- A Baudouin
- A Bellora
- A Bencini
- A Bencini
- A Bencini
- A Betsch
- A Bielschowsky
- A Bielschowsky
- A Birch-Hirschfeld
- A Birch-Hirschfeld
- A Bostroem
- A Bozzoli
- A Busacca
- A Colrat
- A Crocco
- A Cucchia
- A Cucchia
- A Cuneo
- A Czellitzer
- A Escx
- A Favory
- A Favory
- A Favory
- A Favory
- A Feigenbaum
- A Feigenbaum
- A Feigenbaum
- A Feigenbaum
- A Fuchs
- A Fuchs
- A Gelb
- A Glasscheib
- A Glassschera
- A Gordon
- A Greenwood
- A Grönberg
- A Hagedoorn
- A Hagedoorn
- A Holden
- A Jess
- A Jess
- A Jess
- A Jess
- A Jess
- A Josefson
- A Josefson
- A Juba
- A Juba
- A Juba
- A Knapp
- A Knapp
- A Kreiker
- A König
- A Levy
- A Logu
- A Lundberg
- A Lundberg
- A Lundie
- A Lutz
- A Lutz
- A Lutz
- A Lutz
- A Lutz
- A Löwenstein
- A Löwenstein
- A Magitot
- A Magitot
- A Magitot
- A Magitot
- A Magitot
- A Magitot
- A Marin
- A Mccurry
- A Meisling
- A Meyer
- A Monbrun
- A Monbrun
- A Monbrun
- A Neuendorff
- A Nicolato
- A Nieden
- A Nieden
- A Nordenson
- A Passow
- A Patry
- A Paul
- A Pichler
- A Pichler
- A Pick
- A Pietrulla
- A Pillat
- A Pines
- A Reute
- A Rifat
- A Ros
- A Rouquier
- A Rugg-Gunn
- A Rusino
- A Saenger
- A Samoiloff
- A Samoiloff
- A Samoiloff
- A Samoiloff
- A Samoilow
- A Samoilow
- A Santonastaso
- A Santonastaso
- A Santonastaso
- A Sarbo
- A Sargnon
- A Sargnon
- A Sargnon
- A Sargnon
- A Sargnon
- A Schweinitz
- A Schöler
- A Seguini
- A Sinclair
- A Sinclair
- A Sorsby
- A Stargardt
- A Strumpell
- A Sézary
- A Terson
- A Tiscornia
- A Tiscornia
- A Tiscornia
- A Tiscornia
- A Tommasini-Mattiucci
- A Torkildsen
- A Tournay
- A Trettenero
- A Trettenero
- A Vogt
- A Vogt
- A Vogt
- A Volta
- A Wagenmann
- A Weiss
- A Wieczorek
- A Wieczorek
- A Wiener
- A Wiener
- A Zamenhof
- A Zollinger
- Abelsdorff
- Abelsdorff
- AD Purtscher
- Adam
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- Adrian -Matschke
- AL Turner
- Alexander
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- Aloxso , F.
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- Arruga H.
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- B Boros
- B Bramwell
- B Brou
- B Brou
- B Brou
- B Brouwer
- B Castello
- B Cushing
- B Daniels
- B Daniels
- B Fleischer
- B Key
- B Klien
- B Kozlowski
- B Nutta
- B Pommil
- B Ridell
- B Sachs
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- Die Bedeutung der Augenstörungen für die Diagnose der Hirn-und Rückenmarkkrankheiten. Berlin
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- G Kleijn
- G Lenz
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- T Birnbacier
- T Eddison
- T Fay
- T Henderson
- T Iitsuka
- T Inouye
- T Leber
- T Manniewicz
- T Martel
- T Oguchi
- T Oguchi
- T Oguchi
- T Shimada
- T Shimazu
- TB Holloway
- TB Throckmorton
- TD Allan
- TE Davies
- TE Sanders
- TE Schweinitz
- Teleky
- Terrien
- Terrien
- Terrien
- Terrien
- Terrien
- Terrien et Renard
- Terson
- Terson
- Terson
- Terson
- Tertsch
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- TH Buzzard
- TH Johnson
- TH Johnson
- TH Johnson
- TH Klingmann
- TH Leber
- TH Leber
- TH Leber
- TH Leber
- TH Leber
- TH Mohr
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- TH Oliver
- TH Sachs
- TH Sachs
- TH Sachs
- TH Schroeder
- TH Schwarz
- TH Treitel
- TH Treitel
- TH Treitel
- Thies
- Thies
- Thomasson , H.
- Thomasson , H.
- Thomsen
- TJ Putnam
- Toepolt
- Tojoda
- Tomilowa , F.
- Tommasinni Mattiucci , e, Bombelli
- Tornatola
- Traquair
- Traquair
- Traquair
- Treitel
- Treitel
- Triebmeyer
- Trossat
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- Tschiruowsxl
- Tschiruowsxl
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- Turner
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- Uhhtxoff
- Uhhtxoff
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- Uhthoff
- Uhthoff
- Uhthoff
- Uhthoff
- Uhthoff
- Uhthoff
- Uhthoff
- Uhthoff
- Uhthoff
- Uhthoff
- Uhthoff
- Uhthoff
- Ulrich
- Unger
- Urbane
- Urbanek
- v
- V Barletta
- V Benedek
- V Christiansen
- V Christiansen
- V Graefe
- V Graefe
- V Grönholm
- V Hippel
- V Hippel
- V Hippel
- V Hippel
- V Hippel
- V Hippel
- V Hippel
- V Hippel
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- V Leyden
- V Lint
- V Morax
- V Niessl
- V Petragnani
- V Petragnani
- V Rad
- V Reuss
- V Reuss
- V Reuss
- V Ruata
- V Saba
- V Saba
- V Speyr
- V Szily
- V Szily
- V Szily
- v. Benedek
- v. Bogatsch
- v. Eicken
- V. Franklhochwart Topolanski
- v. Graefe
- v. Graefe
- v. Graefe
- v. Graefe
- v. Grosz
- v. Hippel
- v. Hippel
- v. Hippel
- v. Marenholtz
- v. Monakow
- v. Schröder
- v. Sicherer
- V. Szily , sen
- Valière-Vialeix
- Valude
- Van Den Borgh
- Van Der Hoeve
- Van Der Hoeve
- Van Der Hoeve
- Van Der Hoeve
- Van Der Hoeve
- Van Der Hoeve
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- Van Schevensteenfils
- VD Hoeve
- Velhagen
- Vergleichende Blutdruckmessungen an den Gefäßen des Auges
- Verhoeff
- VH Sjögren
- Viereck
- Villard
- Villard
- Vincent et Hartmann
- Vinsonneau
- Vinsonneau
- Visser
- Viterbi
- Vogt
- Volkmann R v
- Vollert
- Vom Hofe
- W Arkin
- W Arkin
- W Binler
- W Braun
- W Craig
- W Cushing
- W Ebstein
- W Eilers
- W Erb
- W Erb
- W Gilbert
- W Grimminger
- W Harris
- W Harris
- W Holden
- W Krauss
- W Köhne
- W Lohlein
- W Ludwig
- W Löhlein
- W Löhlein
- W Mahoney
- W Mahoney
- W Nagel
- W Niemeyer
- W Niemeyer
- W Penfield
- W Rauh
- W Rohrschneider
- W Rönne
- W Scholz
- W Schön
- W Stood
- W Tron
- W Tschirkowsky
- W Tso
- W Tso
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhtiioff
- W Uhtiioff
- W Uhtiioff
- W Uhtiioff
- W Wegner
- W Zeeman
- W Zeeman
- W Zentmayer
- W Zetehlius
- WA Holden
- Wagenmann
- Wagenmann
- Wagenmann
- Wagenmann
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- Walko
- Warlfors
- WB Weidler
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- WE Fry
- WE Fry
- Weber
- Weeks
- Weiser
- Weiss
- Wells Soelberg
- Welton
- Welton
- Welton
- Wessely
- Wessely K.
- Wessely K.
- Wessely K.
- Wessely K.
- Westphal
- Wettendorfer
- WF Norris
- WH Crisp
- WH Melanowski
- Whitehead , L.
- WI Lillie
- WI Lillie
- WI Lillie
- WI Lillie
- WI Lillie
- Wick
- Widmark
- Widmark
- Wiegmann
- Wilbrand
- Wilbrand
- wilbrand
- Williams
- Windsor
- Winselmann
- Wissmann
- Wissmann
- WJ Adie
- WJ Adie
- WJ Hancock
- WL Benedict
- WL Lillie
- WL Lillie
- WL Lillier
- WM Feldman
- Wo1now
- Wolffberg
- Wood
- Woods , W.
- WPC Zeeman
- WR Brain
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- WR Parker
- Wright
- WS Symf
- WT Lister
- Wutso
- Wuttig
- Wölfflin
- Xilo
- Y Cao
- Y Hiriel
- Y Hiriel
- Y Kuwahara
- Y Yoshida
- Z Toth
- Zanotti
- Zeeman
- Zimmermann
- Zirm
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/1944
- Field of study
Lentiviral Vector-Mediated Gene Transfer and RNA Silencing Technology in Neuronal Dysfunctions
- Author
- A Ascherio
- A Bahi
- A Bahi
- A Bahi
- A Bahi
- A Bahi
- A Bahi
- A Bahi
- A Bahi
- A Bahi
- A Bahi
- A Benchoua
- A Benchoua
- A Bilang-Bleuel
- A Bjorklund
- A Burton
- A Caplen
- A Compston
- A Pfeifer
- A Svejgaard
- A Ulusoy
- A Ventura
- AD Ebert
- AE Doyle
- AK Dillon
- AK Halladay
- AP Lieberman
- B Cannella
- B Dass
- B Georgievska
- B Kornek
- C Frank
- C Haute Van den
- C Lavedan
- C LoBianco
- C LoBianco
- C LoBianco
- C Raoul
- C Rosenblad
- C Wersinger
- C Wersinger
- C Winkler
- C Zhao
- CC Chao
- D Charvin
- D Kim
- D Kirik
- D Wu
- D Zala
- D Zala
- DA Hafler
- DA Rubinson
- DC Bragg
- DE Comings
- DL Choi-Lundberg
- DM Dykxhoorn
- DW Black
- E Dowd
- E Nestler
- E Régulier
- EA Nollen
- ED Nelson
- EE Capowski
- EG Willcutt
- EJ Sonuga-Barke
- EW Doss-Pepe
- F Boyer
- F Boyer
- F Weber
- FX Castellanos
- G Bates
- G Cole
- G Frisullo
- G Laviola
- G Porras
- G Rosati
- G Tiscornia
- GJ Hannon
- GP Christophil
- GS Ralph
- H Fukui
- H Jiao
- H Kameda
- H Runne
- H Xia
- J Chen
- J Hewett
- J Jakobsson
- J Janas
- J Jin
- J Szulc
- JC Bensadoun
- JC Dodart
- Jean-Luc Dreyer
- JF Gusella
- JH Kordower
- JH Noseworthy
- JH Richardson
- JL McBride
- JL Ridet
- JM Anaya
- JM Swanson
- JR Crittenden
- JW Hewett
- K Kieburtz
- K Kustedjo
- KL Lim
- KL Richard
- KM Smith
- L Cui
- L Michna
- LJ Ozelius
- LP deAlmeida
- M Azzouz
- M Brizard
- M David
- M Debouverie
- M Dulcan
- M Polymeropoulos
- M Scherr
- M Sierant
- MA Carmell
- MF Beal
- MK Sapru
- MS Brenz-Verca
- N Déglon
- N Kock
- N Popovic
- N Takasugi
- NJ Caplen
- O Singer
- P Aebischer
- P Gonzalez-Alegre
- P Gonzalez-Alegre
- P Gonzalez-Alegre
- P Zhao
- PI Hanson
- PT Massa
- PT Massa
- PT Massa
- R Palacios
- R Spolski
- R Vassar
- RD Oades
- RD Oades
- RE Goodchild
- RG Perez
- S Alves
- S Fahn
- S Gascón
- S Imarisio
- S Mueller-Steiner
- S Palfi
- S Ramaswamy
- S Vergo
- S Wrzesinski
- SA Stewart
- SC Kao
- SD Buckingham
- SJ Gobin
- SM Elbashir
- SQ Harper
- SS El-Amouri
- T Deierborg
- T Dittgen
- T Kitada
- T Kunath
- T Roman
- T Sagvolden
- TE Robinson
- TM Fountaine
- TNM Alerte
- TV Naismith
- V Perrin
- W Adriani
- W Adriani
- WTJ Hendriks
- X Sun
- XM Peng
- Y Imai
- Y Yan
- Y Yue
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study