Health promotion as a subject of the educational curriculum in the Catalan context

OBJETIVO. Comprender la manera en que se ha integrado la salud en el currículum educativo catalán y si ésta ha sido reconocida como una competencia más, contribuyendo con ello al logro de los objetivos de la nueva política europea para la promoción de la salud y el bienestar, denominada “Salud 2020”. Reflexionar acerca del papel pedagógico del profesional de enfermería y proponer alternativas de mejora a la situación actual. MATERIALES Y MÉTODO. Se llevó a cabo un ejercicio interpretativo intencional y contextual articulándose el proceso en varias etapas: delimitación de las áreas de conocimiento curriculares a analizar en las etapas de escolarización obligatoria, redactado de los resultados y análisis de los datos surgidos. Como método analítico se utilizó el denominado análisis de contenido. RESULTADOS. En la etapa de Primaria se observan competencias relacionadas con la salud en los ámbitos de conocimiento del medio; educación física; educación en valores y ámbito digital. En la etapa de Secundaria se observan competencias relacionadas con la salud en los ámbitos cientificotecnológico; social; educación física; cultura y valores; ámbito digital. CONCLUSIONES. la promoción de la salud no se proyecta como una materia más en la que se imparten contenidos de salud, sino como un conjunto de contenidos incluidos transversalmente en algunas de las áreas de conocimiento, lo que supondría una barrera para el conveniente desarrollo de los contenidos de salud. Se propone una nueva materia de promoción de salud, con contenidos adaptados a cada etapa educativa. El profesional de enfermería se erige como idóneo para adquirir la responsabilidad y liderazgo y poner en marcha dicha materiaOBJECTIVE. Understand how health has been integrated into the Catalan educational curriculum and whether it has been recognised as an additional competence, thereby contributing to the achievement of the objectives of the new European policy for the promotion of health and well-being, called "Health 2020". To reflect on the pedagogical role of the nursing professional and to propose alternatives for improvement to the current situation. MATERIALS AND METHODS. An intentional and contextual interpretative process was carried out, articulating the process in several stages: delimitation of the areas of curricular knowledge to be analyzed in the stages of compulsory schooling, writing up the results and data analysis. Content analysis was used as the analytical method. RESULTS. In the Primary Education stage, health-related competencies are observed in the fields of environmental knowledge, physical education, education in moral values and the digital field. At the Secondary level, competencies related to health are observed in the scientific-technological, social, physical education, culture and moral values and digital fields. CONCLUSIONS. Health promotion is not projected as just another subject in which health content is taught, but as a set of contents included transversally in some of the areas of knowledge, which would represent a barrier to the convenient development of health content. A new health promotion subject is proposed, with content adapted to each stage of education. The nursing professional becomes the most suitable choice to acquire responsibility and leadership and to implement this subjec

Detoxification of azo dyes by a novel pH-versatile, salt-resistant laccase from Streptomyces ipomoea

A newly identified extracellular laccase produced by Streptomyces ipomoea CECT 3341 (SilA) was cloned and overexpressed, and its physicochemical characteristics assessed together with its capability to decolorize and detoxify an azotype dye. Molecular analysis of the deduced sequence revealed that SilA contains a TAT-type signal peptide at the N-terminus and only two cupredoxine domains; this is consistent with reports describing two other Streptomyces laccases but contrasts with most laccases, which contain three cupredoxine domains. The heterologous expression and purification of SilA revealed that the homodimer is the only active form of the enzyme. Its stability at high pH and temperature, together with its resistance to high concentrations of NaCl and to typical laccase inhibitors such as sodium azide confirmed the unique properties of this novel laccase. The range of substrates that SilA is able to oxidize was found to be pH-dependent; at alkaline pH, SilA oxidized a wide range of phenolic compounds, including the syringyl and guayacil moieties derived from lignin. The oxidative potential of this enzyme to use phenolic compounds as natural redox mediators was shown through the coordinated action of SilA and acetosyringone (as mediator), which resulted in the complete detoxification of the azo-type dye Orange II.This work was financially supported by the Spanish Ministry of Science (CICYT Project CTQ2004-0344/PPQ to MEA and BFU 2006-00972/BMC to JMD). We thank the Autonomous Government of Madrid and the University of Alcalá for the fellowship awarded to R.M

Delphi Study about the educational skills by the School Nursing Professional

Objetivo: definir al profesional de Enfermería promotor de salud en el entorno escolar (PEPSEE), así como su perfil competencial pedagógico: actitudes, habilidades y conocimientos necesarios para llevar a cabo una acción de calidad y efectiva en dicho entorno. Método: se planteó una investigación de tipo cualitativa, de corte interpretativo y diseño mixto. En la recogida de datos se combinó la revisión documental con el método Delphi como técnica basada en cuestionarios a expertos, cuyo desarrollo quedó establecido en tres rondas. Hubo diferentes momentos de análisis en función de la tipología de los cuestionarios que se aplicaron, combinándose el análisis de contenido con el análisis estadístico descriptivo. Por último, se efectuó un proceso de relación y comparación de los datos obtenidos, procediendo al redactado de los resultados. Resultados: se extrajeron 11 rasgos definitorios para el PEPSEE delimitados en tres categorías: rol educador, visión integral del educando y enfoque integrador de la acción, y 81 elementos competenciales delimitados en nueve categorías: conocimiento base para la enseñanza de salud en la escuela, competencia interpersonal y de trabajo en equipo, habilidades de mediación pedagógica de salud, comunicación educativa, actitud docente competente, valores profesionales inherentes a la dimensión ética, investigación pedagógica en promoción de salud, competencia digital e innovación pedagógica, coordinación y liderazgo educativo. Conclusiones: el profesional de Enfermería promotor de salud en el entorno escolar es un profesional con identidad propia que debe ser reconocido social y profesionalmente como garante de una formación en salud de calidad

PRPF8-mediated dysregulation of hBrr2 helicase disrupts human spliceosome kinetics and 5\ub4-splice-site selection causing tissue-specific defects

\ua9 The Author(s) 2024.The carboxy-terminus of the spliceosomal protein PRPF8, which regulates the RNA helicase Brr2, is a hotspot for mutations causing retinitis pigmentosa-type 13, with unclear role in human splicing and tissue-specificity mechanism. We used patient induced pluripotent stem cells-derived cells, carrying the heterozygous PRPF8 c.6926 A > C (p.H2309P) mutation to demonstrate retinal-specific endophenotypes comprising photoreceptor loss, apical-basal polarity and ciliary defects. Comprehensive molecular, transcriptomic, and proteomic analyses revealed a role of the PRPF8/Brr2 regulation in 5’-splice site (5’SS) selection by spliceosomes, for which disruption impaired alternative splicing and weak/suboptimal 5’SS selection, and enhanced cryptic splicing, predominantly in ciliary and retinal-specific transcripts. Altered splicing efficiency, nuclear speckles organisation, and PRPF8 interaction with U6 snRNA, caused accumulation of active spliceosomes and poly(A)+ mRNAs in unique splicing clusters located at the nuclear periphery of photoreceptors. Collectively these elucidate the role of PRPF8/Brr2 regulatory mechanisms in splicing and the molecular basis of retinal disease, informing therapeutic approaches

Single hadron response measurement and calorimeter jet energy scale uncertainty with the ATLAS detector at the LHC

The uncertainty on the calorimeter energy response to jets of particles is derived for the ATLAS experiment at the Large Hadron Collider (LHC). First, the calorimeter response to single isolated charged hadrons is measured and compared to the Monte Carlo simulation using proton-proton collisions at centre-of-mass energies of sqrt(s) = 900 GeV and 7 TeV collected during 2009 and 2010. Then, using the decay of K_s and Lambda particles, the calorimeter response to specific types of particles (positively and negatively charged pions, protons, and anti-protons) is measured and compared to the Monte Carlo predictions. Finally, the jet energy scale uncertainty is determined by propagating the response uncertainty for single charged and neutral particles to jets. The response uncertainty is 2-5% for central isolated hadrons and 1-3% for the final calorimeter jet energy scale.Comment: 24 pages plus author list (36 pages total), 23 figures, 1 table, submitted to European Physical Journal

Jet energy measurement with the ATLAS detector in proton-proton collisions at root s=7 TeV

The jet energy scale and its systematic uncertainty are determined for jets measured with the ATLAS detector at the LHC in proton-proton collision data at a centre-of-mass energy of √s = 7TeV corresponding to an integrated luminosity of 38 pb-1. Jets are reconstructed with the anti-kt algorithm with distance parameters R=0. 4 or R=0. 6. Jet energy and angle corrections are determined from Monte Carlo simulations to calibrate jets with transverse momenta pT≥20 GeV and pseudorapidities {pipe}η{pipe}<4. 5. The jet energy systematic uncertainty is estimated using the single isolated hadron response measured in situ and in test-beams, exploiting the transverse momentum balance between central and forward jets in events with dijet topologies and studying systematic variations in Monte Carlo simulations. The jet energy uncertainty is less than 2. 5 % in the central calorimeter region ({pipe}η{pipe}<0. 8) for jets with 60≤pT<800 GeV, and is maximally 14 % for pT<30 GeV in the most forward region 3. 2≤{pipe}η{pipe}<4. 5. The jet energy is validated for jet transverse momenta up to 1 TeV to the level of a few percent using several in situ techniques by comparing a well-known reference such as the recoiling photon pT, the sum of the transverse momenta of tracks associated to the jet, or a system of low-pT jets recoiling against a high-pT jet. More sophisticated jet calibration schemes are presented based on calorimeter cell energy density weighting or hadronic properties of jets, aiming for an improved jet energy resolution and a reduced flavour dependence of the jet response. The systematic uncertainty of the jet energy determined from a combination of in situ techniques is consistent with the one derived from single hadron response measurements over a wide kinematic range. The nominal corrections and uncertainties are derived for isolated jets in an inclusive sample of high-pT jets. Special cases such as event topologies with close-by jets, or selections of samples with an enhanced content of jets originating from light quarks, heavy quarks or gluons are also discussed and the corresponding uncertainties are determined. © 2013 CERN for the benefit of the ATLAS collaboration

Multiplex RNA-based detection of clinically relevant MET alterations in advanced non-small cell lung cancer

We studied MET alterations in 474 advanced non-small-cell lung cancer (NSCLC) patients by nCounter, an RNA-based technique. We identified 3% with MET Δex14 mRNA and 3.5% with very-high MET mRNA expression, a surrogate of MET amplification. MET alterations identified by nCounter correlated with clinical benefit from MET inhibitors. Quantitative mRNA-based techniques can improve the selection of patients for MET-targeted therapies. MET inhibitors have shown activity in non-small-cell lung cancer patients (NSCLC) with MET amplification and exon 14 skipping (METΔex14). However, patient stratification is imperfect, and thus, response rates have varied widely. Here, we studied MET alterations in 474 advanced NSCLC patients by nCounter, an RNA-based technique, together with next-generation sequencing (NGS), fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), and reverse transcriptase polymerase chain reaction (RT-PCR), exploring correlation with clinical benefit. Of the 474 samples analyzed, 422 (89%) yielded valid results by nCounter, which identified 13 patients (3%) with MET Δex14 and 15 patients (3.5%) with very-high MET mRNA expression. These two subgroups were mutually exclusive, displayed distinct phenotypes and did not generally coexist with other drivers. For MET Δex14, 3/8 (37.5%) samples positive by nCounter tested negative by NGS. Regarding patients with very-high MET mRNA, 92% had MET amplification by FISH and/or NGS. However, FISH failed to identify three patients (30%) with very-high MET RNA expression, among which one received MET tyrosine kinase inhibitor treatment deriving clinical benefit. Our results indicate that quantitative mRNA-based techniques can improve the selection of patients for MET-targeted therapies

She\u27s So Bubbly

We introduce the Automatic Learning for the Rapid Classification of Events (ALeRCE) broker, an astronomical alert broker designed to provide a rapid and self-consistent classification of large etendue telescope alert streams, such as that provided by the Zwicky Transient Facility (ZTF) and, in the future, the Vera C. Rubin Observatory Legacy Survey of Space and Time (LSST). ALeRCE is a Chilean-led broker run by an interdisciplinary team of astronomers and engineers working to become intermediaries between survey and follow-up facilities. ALeRCE uses a pipeline that includes the real-time ingestion, aggregation, cross-matching, machine-learning (ML) classification, and visualization of the ZTF alert stream. We use two classifiers: a stamp-based classifier, designed for rapid classification, and a light curve–based classifier, which uses the multiband flux evolution to achieve a more refined classification. We describe in detail our pipeline, data products, tools, and services, which are made public for the community (see https://alerce.science). Since we began operating our real-time ML classification of the ZTF alert stream in early 2019, we have grown a large community of active users around the globe. We describe our results to date, including the real-time processing of 1.5 × 10⁸ alerts, the stamp classification of 3.4 × 10⁷ objects, the light-curve classification of 1.1 × 10⁶ objects, the report of 6162 supernova candidates, and different experiments using LSST-like alert streams. Finally, we discuss the challenges ahead in going from a single stream of alerts such as ZTF to a multistream ecosystem dominated by LSST