Cochleates derived from Vibrio cholerae O1 proteoliposomes : The impact of structure transformation on mucosal immunisation

Cochleates are phospholipid-calcium precipitates derived from the interaction of anionic lipid vesicles with divalent cations. Proteoliposomes from bacteria may also be used as a source of negatively charged components, to induce calcium-cochleate formation. In this study, proteoliposomes from V. cholerae O1 (PLc) (sized 160.7±1.6 nm) were transformed into larger (16.3±4.6 µm) cochleate-like structures (named Adjuvant Finlay Cochleate 2, AFCo2) and evaluated by electron microscopy (EM). Measurements from transmission EM (TEM) showed the structures had a similar size to that previously reported using light microscopy, while observations from scanning electron microscopy (SEM) indicated that the structures were multilayered and of cochleate-like formation. The edges of the AFCo2 structures appeared to have spaces that allowed penetration of negative stain or Ovalbumin labeled with Texas Red (OVA-TR) observed by epi-fluorescence microscopy. In addition, freeze fracture electron microscopy confirmed that the AFCo2 structures consisted of multiple overlapping layers, which corresponds to previous descriptions of cochleates. TEM also showed that small vesicles co-existed with the larger cochleate structures, and in vitro treatment with a calcium chelator caused the AFCo2 to unfold and reassemble into small proteoliposome-like structures. Using OVA as a model antigen, we demonstrated the potential loading capacity of a heterologous antigen and in vivo studies showed that with simple admixing and administration via intragastric and intranasal routes AFCo2 provided enhanced adjuvant properties compared with PLc

A large, massive, rotating disk around an isolated young stellar object

We present multi-wavelengths observations and a radiative transfer model of a newly discovered massive circumstellar disk of gas and dust which is one of the largest disks known today. Seen almost edge-on, the disk is resolved in high-resolution near-infrared (NIR) images and appears as a dark lane of high opacity intersecting a bipolar reflection nebula. Based on molecular line observations we estimate the distance to the object to be 3.5 kpc. This leads to a size for the dark lane of ~10500 AU but due to shadowing effects the true disk size could be smaller. In Spitzer/IRAC 3.6 micron images the elongated shape of the bipolar reflection nebula is still preserved and the bulk of the flux seems to come from disk regions that can be detected due to the slight inclination of the disk. At longer IRAC wavelengths, the flux is mainly coming from the central regions penetrating directly through the dust lane. Interferometric observations of the dust continuum emission at millimeter wavelengths with the SMA confirm this finding as the peak of the unresolved mm-emission coincides perfectly with the peak of the Spitzer/IRAC 5.8 micron flux and the center of the dark lane seen in the NIR images. Simultaneously acquired CO data reveal a molecular outflow along the northern part of the reflection nebula which seems to be the outflow cavity. An elongated gaseous disk component is also detected and shows signs of rotation. The emission is perpendicular to the molecular outflow and thus parallel to but even more extended than the dark lane in the NIR images. Based on the dust continuum and the CO observations we estimate a disk mass of up to a few solar masses depending on the underlying assumptions. Whether the disk-like structure is an actual accretion disk or rather a larger-scale flattened envelope or pseudodisk is difficult to discriminate with the current dataset (abridged).Comment: Accepted for publication in ApJ, 29 pages preprint style incl. 10 Figure

Returning home: heritage work among the Stl'atl'imx of the Lower Lillooet River Valley

This article focusses on heritage practices in the tensioned landscape of the Stl’atl’imx (pronounced Stat-lee-um) people of the Lower Lillooet River Valley, British Columbia, Canada. Displaced from their traditional territories and cultural traditions through the colonial encounter, they are enacting, challenging and remaking their heritage as part of their long term goal to reclaim their land and return ‘home’. I draw on three examples of their heritage work: graveyard cleaning, the shifting ‘official’/‘unofficial’ heritage of a wagon road, and marshalling of the mountain named Nsvq’ts (pronounced In-SHUCK-ch) in order to illustrate how the past is strategically mobilised in order to substantiate positions in the present. While this paper focusses on heritage in an Indigenous and postcolonial context, I contend that the dynamics of heritage practices outlined here are applicable to all heritage practices

Vaccinating adolescents against SARS-CoV-2 in England: a risk-benefit analysis.

OBJECTIVE: To offer a quantitative risk-benefit analysis of two doses of SARS-CoV-2 vaccination among adolescents in England. SETTING: England. DESIGN: Following the risk-benefit analysis methodology carried out by the US Centers for Disease Control, we calculated historical rates of hospital admission, Intensive Care Unit admission and death for ascertained SARS-CoV-2 cases in children aged 12-17 in England. We then used these rates alongside a range of estimates for incidence of long COVID, vaccine efficacy and vaccine-induced myocarditis, to estimate hospital and Intensive Care Unit admissions, deaths and cases of long COVID over a period of 16 weeks under assumptions of high and low case incidence. PARTICIPANTS: All 12-17 year olds with a record of confirmed SARS-CoV-2 infection in England between 1 July 2020 and 31 March 2021 using national linked electronic health records, accessed through the British Heart Foundation Data Science Centre. MAIN OUTCOME MEASURES: Hospitalisations, Intensive Care Unit admissions, deaths and cases of long COVID averted by vaccinating all 12-17 year olds in England over a 16-week period under different estimates of future case incidence. RESULTS: At high future case incidence of 1000/100,000 population/week over 16 weeks, vaccination could avert 4430 hospital admissions and 36 deaths over 16 weeks. At the low incidence of 50/100,000/week, vaccination could avert 70 hospital admissions and two deaths over 16 weeks. The benefit of vaccination in terms of hospitalisations in adolescents outweighs risks unless case rates are sustainably very low (below 30/100,000 teenagers/week). Benefit of vaccination exists at any case rate for the outcomes of death and long COVID, since neither have been associated with vaccination to date. CONCLUSIONS: Given the current (as at 15 September 2021) high case rates (680/100,000 population/week in 10-19 year olds) in England, our findings support vaccination of adolescents against SARS-CoV2

Smoothly-Rising Star Formation Histories During the Reionization Epoch

Cosmological hydrodynamic simulations robustly predict that high-redshift galaxy star formation histories (SFHs) are smoothly-rising and vary with mass only by a scale factor. We use our latest simulations to test whether this scenario can account for recent observations at z>=6 from WFC3/IR, NICMOS, and IRAC. Our simulations broadly reproduce the observed ultraviolet (UV) luminosity functions and stellar mass densities and their evolution at z=6-8, all of which are nontrivial tests of the mean SFH. In agreement with observations, simulated galaxies possess blue UV continua owing to young ages (50-150 Myr), low metallicities (0.1-0.5 Zsun), and low dust columns (E(B-V) <= 0.05). Observations imply a near-unity slope in the stellar mass--star formation rate relation at all z=6-8, confirming the prediction that SFH shapes are invariant. Current surveys detect the majority of galaxies with stellar masses exceeding 10^9 Msun and few galaxies less massive than 10^{8.5} Msun, implying that they probe no more than the brightest 30% of the complete star formation and stellar mass densities at z>=6. Finally, we demonstrate that there is no conflict between smoothly-rising SFHs and recent clustering observations. This is because momentum-driven outflows suppress star formation in low-mass halos, leading to overall occupancies of 0.2-0.4 even though the star formation duty cycle is one. This leads to many interesting predictions at z>=4, among them that (1) optically-selected and UV-selected samples largely overlap; (2) few galaxies exhibit significantly suppressed specific star formation rates; and (3) occupancy is constant or increasing with decreasing luminosity. These predictions are in tentative agreement with current observations, but further analysis of existing and upcoming data sets is required in order to test them more thoroughly. (abridged)Comment: 25 pages, 14 figures, accepted to MNRA

X Chromosome Inactivation during Drosophila Spermatogenesis

Genes with male- and testis-enriched expression are under-represented on the Drosophila melanogaster X chromosome. There is also an excess of retrotransposed genes, many of which are expressed in testis, that have “escaped” the X chromosome and moved to the autosomes. It has been proposed that inactivation of the X chromosome during spermatogenesis contributes to these patterns: genes with a beneficial function late in spermatogenesis should be selectively favored to be autosomal in order to avoid inactivation. However, conclusive evidence for X inactivation in the male germline has been lacking. To test for such inactivation, we used a transgenic construct in which expression of a lacZ reporter gene was driven by the promoter sequence of the autosomal, testis-specific ocnus gene. Autosomal insertions of this transgene showed the expected pattern of male- and testis-specific expression. X-linked insertions, in contrast, showed only very low levels of reporter gene expression. Thus, we find that X linkage inhibits the activity of a testis-specific promoter. We obtained the same result using a vector in which the transgene was flanked by chromosomal insulator sequences. These results are consistent with global inactivation of the X chromosome in the male germline and support a selective explanation for X chromosome avoidance of genes with beneficial effects late in spermatogenesis

Star-forming cores embedded in a massive cold clump: Fragmentation, collapse and energetic outflows

The fate of massive cold clumps, their internal structure and collapse need to be characterised to understand the initial conditions for the formation of high-mass stars, stellar systems, and the origin of associations and clusters. We explore the onset of star formation in the 75 M_sun SMM1 clump in the region ISOSS J18364-0221 using infrared and (sub-)millimetre observations including interferometry. This contracting clump has fragmented into two compact cores SMM1 North and South of 0.05 pc radius, having masses of 15 and 10 M_sun, and luminosities of 20 and 180 L_sun. SMM1 South harbours a source traced at 24 and 70um, drives an energetic molecular outflow, and appears supersonically turbulent at the core centre. SMM1 North has no infrared counterparts and shows lower levels of turbulence, but also drives an outflow. Both outflows appear collimated and parsec-scale near-infrared features probably trace the outflow-powering jets. We derived mass outflow rates of at least 4E-5 M_sun/yr and outflow timescales of less than 1E4 yr. Our HCN(1-0) modelling for SMM1 South yielded an infall velocity of 0.14 km/s and an estimated mass infall rate of 3E-5 M_sun/yr. Both cores may harbour seeds of intermediate- or high-mass stars. We compare the derived core properties with recent simulations of massive core collapse. They are consistent with the very early stages dominated by accretion luminosity.Comment: Accepted for publication in ApJ, 14 pages, 7 figure

Rectal Prolapse: Review According to the Personal Experience

The aim of treatment of rectal prolapse is to control the prolapse, restore continence, and prevent constipation or impaired evacuation. Faced with a multitude of options, the choice of an optimal treatment is difficult. It is best tailored to patient and surgeon. Numerous procedures have been described and are generally categorized into perineal or abdominal approaches. In general, an abdominal procedure has associated with lower recurrence and better functional outcome than perineal procedures. The widespread success of laparoscopic surgery has led to the development of laparoscopic procedures in the treatment of complete rectal prolapse. In Korea, there has been a trend toward offering perineal procedures because of the high incidence of rectal prolapse in young males and its being a lesser procedure. Delorme-Thiersch procedure has appeal as a lesser procedure for patients of any age or risk category, especially for elderly low-risk patients, patients with constipation or evacuation difficulties, young males, and patients with symptomatic hemorrhoids or mucosal prolapse. Laparoscopic suture rectopexy is recommended for either low-risk female patients or patients who are concerned with postoperative aggravation of their incontinence