VPPA weld model evaluation

NASA uses the Variable Polarity Plasma Arc Welding (VPPAW) process extensively for fabrication of Space Shuttle External Tanks. This welding process has been in use at NASA since the late 1970's but the physics of the process have never been satisfactorily modeled and understood. In an attempt to advance the level of understanding of VPPAW, Dr. Arthur C. Nunes, Jr., (NASA) has developed a mathematical model of the process. The work described in this report evaluated and used two versions (level-0 and level-1) of Dr. Nunes' model, and a model derived by the University of Alabama at Huntsville (UAH) from Dr. Nunes' level-1 model. Two series of VPPAW experiments were done, using over 400 different combinations of welding parameters. Observations were made of VPPAW process behavior as a function of specific welding parameter changes. Data from these weld experiments was used to evaluate and suggest improvements to Dr. Nunes' model. Experimental data and correlations with the model were used to develop a multi-variable control algorithm for use with a future VPPAW controller. This algorithm is designed to control weld widths (both on the crown and root of the weld) based upon the weld parameters, base metal properties, and real-time observation of the crown width. The algorithm exhibited accuracy comparable to that of the weld width measurements for both aluminum and mild steel welds

Teaching Livestock Producers to Use Handheld Computers

Mandatory animal ID is a well-discussed topic among livestock producers. A national ID program will probably require animal records to be maintained in electronic format. A 2-day intensive training was held to help cattle producers become proficient in using a handheld computer for animal record keeping. On an end-of-meeting evaluation, 99% of the participants felt a handheld computer would make record keeping tasks easier. A follow-up survey was mailed 1 year after the program generated a 70% response rate. After one year, only 57% of the participants felt that the handheld computer made their record-keeping task easier

Chemotactic Migration of Clustered Central Nervous System Progenitor Cells

Clustering of central nervous system (CNS) cells is often utilized for cell growth and characterization, as well as investigated for tissue regeneration and disease progression. Collective CNS cell migration, however, has been largely unstudied. Cell cluster formation and migration play a critical part of modeling in vivo conditions and in development of therapies. Three distinct CNS cell types, medulloblastoma (MB), medulloblastoma-derived glial progenitor cells (MGPC), and retinal progenitor cells (RPC), were investigated for cluster formation, upregulation of CXCR4, the receptor for Stromal-Derived Growth Factor (SDF-1), and Connexin 43 expression, a gap junction hemichannel. A microfluidic platform was used to examine the the migration of clusters and single cells in response to controlled concentration gradients of SDF-1. All cell types illustrated self-clustering, as well as upregulated CXCR4 surface expression and increased Connexin 43 expression upon ligand stimulation. Further, RPC clusters exhibited collective, chemotactic migration along SDF-1 concentration gradients, while MB clusters illustrated inconsistent collective migration, and MGPCs clusters did not exhibit adhesion-based migration

Comparison of honey bee queens overwintered individually and in groups

Productivity of honey bee queens, as measured by area of sealed worker brood and net weight of colonies, was generally higher with queens overwintered in 2-frame nuclei, than with queens overwintered in a group. Poor acceptance and supercedure of group overwintered queens suggest that this method of storage is not yet acceptable for commercial use. Survival of the nucleus queens was low in outdoor 2-frame units during the winter, but improved with an indoor system. Overwintering queens indoors in 2-frame nuclei and outdoors in 3-5 frame nuclei with supplemental feeding of carbohydrate in late winter should provide a source of queens which could partially fulfill market demands in spring

In vitro formation of neuroclusters in microfluidic devices and cell migration as a function of stromal-derived growth factor 1 gradients

Central nervous system (CNS) cells cultured in vitro as neuroclusters are useful models of tissue regeneration and disease progression. However, the role of cluster formation and collective migration of these neuroclusters to external stimuli has been largely unstudied in vitro. Here, 3 distinct CNS cell types, medulloblastoma (MB), medulloblastoma-derived glial progenitor cells (MGPC), and retinal progenitor cells (RPC), were examined with respect to cluster formation and migration in response to Stromal-Derived Growth Factor (SDF-1). A microfluidic platform was used to distinguish collective migration of neuroclusters from that of individual cells in response to controlled concentration profiles of SDF-1. Cell lines were also compared with respect to expression of CXCR4, the receptor for SDF-1, and the gap junction protein Connexin 43 (Cx43). All cell types spontaneously formed clusters and expressed both CXCR4 and Cx43. RPC clusters exhibited collective chemotactic migration (i.e. movement as clusters) along SDF-1 concentration gradients. MGPCs clusters did not exhibit adhesion-based migration, and migration of MB clusters was inconsistent. This study demonstrates how controlled microenvironments can be used to examine the formation and collective migration of CNS-derived neuroclusters in varied cell populations

Off-target capture data, endosymbiont genes and morphology reveal a relict lineage that is sister to all other singing cicadas

Phylogenetic asymmetry is common throughout the tree of life and results from contrasting patterns of speciation and extinction in the paired descendant lineages of ancestral nodes. On the depauperate side of a node, we find extant ´relict´ taxa that sit atop long, unbranched lineages. Here, we show that a tiny, pale green, inconspicuous and poorly known cicada in the genus Derotettix, endemic to degraded salt-plain habitats in arid regions of central Argentina, is a relict lineage that is sister to all other modern cicadas. Nuclear and mitochondrial phylogenies of cicadas inferred from probe-based genomic hybrid capture data of both target and non-target loci and a morphological cladogram support this hypothesis. We strengthen this conclusion with genomic data from one of the cicada nutritional bacterial endosymbionts, Sulcia, an ancient and obligate endosymbiont of the larger plant-sucking bugs (Auchenorrhyncha) and an important source of maternally inherited phylogenetic data. We establish Derotettiginae subfam. nov. as a new, monogeneric, fifth cicada subfamily, and compile existing and new data on the distribution, ecology and diet of Derotettix. Our consideration of the palaeoenvironmental literature and host-plant phylogenetics allows us to predict what might have led to the relict status of Derotettix over 100 Myr of habitat change in South America.Fil: Simon, Chris. University of Connecticut; Estados UnidosFil: Gordon, Eric R. L.. University of Connecticut; Estados UnidosFil: Moulds, M.S.. Australian Museum Research Institute; AustraliaFil: Cole, Jeffrey A.. Pasadena City College; Estados UnidosFil: Haji, Diler. University of Connecticut; Estados UnidosFil: Lemmon, Alan R.. Florida State University; Estados UnidosFil: Lemmon, Emily Moriarty. Florida State University; Estados UnidosFil: Kortyna, Michelle. Florida State University; Estados UnidosFil: Nazario, Katherine. University of Connecticut; Estados UnidosFil: Wade, Elizabeth J.. Curry College. Department of Natural Sciences and Mathematics; Estados Unidos. University of Connecticut; Estados UnidosFil: Meister, Russell C.. University of Connecticut; Estados UnidosFil: Goemans, Geert. University of Connecticut; Estados UnidosFil: Chiswell, Stephen M.. National Institute of Water and Atmospheric Research; Nueva ZelandaFil: Pessacq, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; ArgentinaFil: Veloso, Claudio. Universidad de Chile; ChileFil: McCutcheon, John P.. University of Montana; Estados UnidosFil: Lukasik, Piotr. University of Montana; Estados Unidos. Swedish Museum of Natural History. Department of Bioinformatics and Genetics; Sueci

A near IR imaging survey of intermediate and high-mass young stellar outflow candidates

We have carried out a near-infrared imaging survey of luminous young stellar outflow candidates using the United Kingdom Infrared Telescope. Observations were obtained in the broad band K (2.2 mu) and through narrow band filters at the wavelengths of H_2 v=1--0 S(1) (2.1218 mu) and Br gamma (2.166 mu) lines. Fifty regions were imaged with a field of view of 2.2 X 2.2 arcmin^2. Several young embedded clusters are unveiled in our near-infrared images. 76% of the objects exhibit H_2 emission and 50% or more of the objects exhibit aligned H_2 emission features suggesting collimated outflows, many of which are new detections. These observations suggest that disk accretion is probably the leading mechanism in the formation of stars, at least up to late O spectral types. The young stellar objects responsible for many of these outflows are positively identified in our images based on their locations with respect to the outflow lobes, 2MASS colours and association with MSX, IRAS, millimetre and radio sources. The close association of molecular outflows detected in CO with the H_2 emission features produced by shock excitation by jets from the young stellar objects suggests that the outflows from these objects are jet-driven. Towards strong radio emitting sources, H_2 jets were either not detected or were weak when detected, implying that most of the accretion happens in the pre-UCHII phase; accretion and outflows are probably weak when the YSO has advanced to its UCHII stage.Comment: 64 pages, 53 figures, Accepted for publication in the MNRA

An automatic analysis framework for FDOPA PET neuroimaging

In this study we evaluate the performance of a fully automated analytical framework for FDOPA PET neuroimaging data, and its sensitivity to demographic and experimental variables and processing parameters. An instance of XNAT imaging platform was used to store the King's College London institutional brain FDOPA PET imaging archive, alongside individual demographics and clinical information. By re-engineering the historical Matlab-based scripts for FDOPA PET analysis, a fully automated analysis pipeline for imaging processing and data quantification was implemented in Python and integrated in XNAT. The final data repository includes 892 FDOPA PET scans organized from 23 different studies. We found good reproducibility of the data analysis by the automated pipeline (in the striatum for the Kicer: for the controls ICC = 0.71, for the psychotic patients ICC = 0.88). From the demographic and experimental variables assessed, gender was found to most influence striatal dopamine synthesis capacity (F = 10.7, p < 0.001), with women showing greater dopamine synthesis capacity than men. Our automated analysis pipeline represents a valid resourse for standardised and robust quantification of dopamine synthesis capacity using FDOPA PET data. Combining information from different neuroimaging studies has allowed us to test it comprehensively and to validate its replicability and reproducibility performances on a large sample size