Mechanical Wastewater Facility Challenges in the Canadian Arctic

Abstract The consistent performance of wastewater treatment in the far north of Canada, in general, remains an elusive objective, and a frustration for engineers, communities, senior governments, and regulators. Lagoon systems suffer from performance inconsistencies, and a significant scientific effort has been underway by the Government of Nunavut to study and predict the performance of lagoon systems. It has been pointed out that those systems which are technologically simple, and engineered for sufficient capacity tend to perform well, however lagoon systems are ultimately at the mercy of the natural environment, which is extreme in the far north. Mechanical systems do offer the opportunity to reduce the influence of the natural environment, however a multitude of other factors affect the design, construction, operation and maintenance of mechanical systems in the far north. As an opportunity to mitigate the challenges associated with mechanical wastewater systems, a synopsis of the community mechanical treatment facilities in the north has been compiled. Lessons learned from the challenges with mechanical wastewater systems in the far north have been catalogued as a legacy document to future project stakeholders. This compilation is a first attempt to provide documentation to serve as a reference for improving the development, execution, and operation of future mechanical wastewater treatment projects, where this technical option is deemed appropriate

Free Radical Scavenging and In vitro Cytotoxic Activity of Bugnay (Antidesma bunius) Leaves Extract against A549 Human Lung Adenocarcinoma and HCT-116 Human Colorectal Cancer Cell Lines

Cancer is one of the significant causes of mortality worldwide. Studies on antineoplastic drugs focused on natural products have revealed several mechanisms to inhibit cancer cells. Bugnay (Antidesma bunius) leaves showed potentials due to its activity observed against brine shrimp and breast cancer cells. However, there is still limited knowledge about its activity against other human cancer cells. This study focused on determining the phytochemical compounds in A. bunius leaves extract, the free radical scavenging activity of the extract using the Diphenylpicrylhydrazyl (DPPH) method, and in vitro cytotoxic activity against two cancer cell lines, namely HCT-116 human colorectal and A549 human lung adenocarcinoma cancer cell lines by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemicals identified were unsaturated lactones, flavonoids, phenolics, diterpenes, saponins, tannins, carbohydrates, and reducing sugars. The extract showed significant free radical scavenging activity and a direct correlation of activity with concentration levels. It also exhibited cytotoxic activity against HCT-116 human colorectal and A549 human lung adenocarcinoma. Hence, A. bunius leaves have the potential to be a source of antioxidant and antineoplastic compounds. This warrant further isolation of the compounds for chemotherapeutic purposes.Keywords: Antidesma bunius, Bugnay, Cancer, Cytotoxicity, Radica