14 research outputs found
Development of novel delivery systems for nose-to-brain drug delivery
The blood brain barrier (BBB) poses a significant hurdle to brain drug delivery. However, the location of the olfactory mucosa, within the nasal cavity, is a viable target site for direct nose-to-brain (N2B) delivery, thereby bypassing the BBB. To exploit this target site innovative nasal formulations are required for targeting and increasing residency within the olfactory mucosa. We developed and characterised three formulation systems for N2B delivery, (i) thermoresponsive mucoadhesion nasal gels sprays; (ii) mesoporous silica nanoparticles and (iii) nasal pMDI devices. We developed an optimal mucoadhesive formulation system incorporating amantadine as a model, water-soluble anti-Parkinson’s drug using carboxymethy cellulose and chitosan as mucoadhesives. Formulations demonstrated droplet sizes of < 130mm and stability over 8-weeks when stored at refrigeration conditions with no significant cellular toxicity against olfactory bulb (OBGF400) and nasal epithelial (RPMI 2650) cells. Mesoporous silica nanoparticles (MSNP) were prepared (~220nm) and demonstrated cellular uptake into OBGF400 within 2-hours of incubation with minimal toxicity. MSNP were loaded with two novel phytochemicals known to possess CNS activity, curcumin and chrysin, with loading efficiencies of ~12% confirmed through TGA, DSC and HPLC-UV analysis. Furthermore, a pH dependant release profile was identified with curcumin with greater release at nasal cavity pH 5.5 compared to pH 7.4. Furthermore, successful incorporation of MSNP into nasal gels was demonstrated through rheological studies with a decrease in Tsol-gel. A pilot study was conducted to assess the feasibility of modified existing pulmonary pMDI to deliver diazepam intranasally, targeting the olfactory mucosa. Diazepam was formulated with HFA134a and using ethanol as a co-solvent, and demonstrated stability in formulation over 3 months. Deposition studies within a nasal cast model demonstrated 5-6% deposition onto the olfactory mucosa under optimal administration conditions in the absence of any nozzle attachments. Our studies have provided a basis for the development to innovative intranasal formulation systems potentially capable of targeting the olfactory mucosa for both water soluble and poorly soluble drugs
Development and Evaluation of a Novel Intranasal Spray for the Delivery of Amantadine
The aim of this study was to develop and characterise an intranasal delivery system for amantadine (AMT). Optimal formulations (F) consisted of a thermosensitive polymer Pluronic® 127 (P127) and either carboxy methyl cellulose (CMC) or chitosan (CS) which demonstrated gel-transition at nasal cavity temperatures (34 °C ± 1 °C). Rheologically, the loss tangent (Tan δ) confirmed a three-stage gelation phenomena at 34 °C ± 1 °C and non-Newtonian behaviour. Storage of FCMC and FCS at 4 °C for 8 weeks resulted in repeatable release profiles at 34 °C when sampled, with a Fickian mechanism earlier on but moving towards anomalous transport by week 8. Polymers (P127, CMC and CS) demonstrated no significant cellular toxicity to human nasal epithelial cells up to 4 mg/mL and up to 1 mM for AMT (IC50: 4.5 mM ± 0.05 mM). FCMC and FCS demonstrated slower release across an in-vitro human nasal airway model (43-44 % vs 79 % ± 4.58 % for AMT). Using a human nasal cast model, deposition into the olfactory regions (potential nose-to-brain) was demonstrated upon nozzle insertion (5 mm) whereas tilting of the head forward (15°) resulted in greater deposition in the bulk of the nasal cavity
Phytochemical-loaded mesoporous silica nanoparticles for nose-to-brain olfactory drug delivery
Central nervous system (CNS) drug delivery is often hampered due to the insidious nature of the blood-brain barrier (BBB). Nose-to-brain delivery via olfactory pathways have become a target of attention for drug delivery due to bypassing of the BBB. The antioxidant properties of phytochemicals make them promising as CNS active agents but possess poor water solubility and limited BBB penetration. The primary aim of this study was the development of mesoporous silica nanoparticles (MSNs) loaded with the poorly water-soluble phytochemicals curcumin and chrysin which could be utilised for nose-to-brain delivery. We formulated spherical MSNP using a templating approach resulting in ∼220nm particles with a high surface porosity. Curcumin and chrysin were successfully loaded into MSNP and confirmed through Fourier transformation infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and HPLC approaches with a loading of 11-14% for curcumin and chrysin. Release was pH dependant with curcumin demonstrating increased chemical stability at a lower pH (5.5) with a release of 53.2%±2.2% over 24h and 9.4±0.6% for chrysin. MSNP were demonstrated to be non-toxic to olfactory neuroblastoma cells OBGF400, with chrysin (100μM) demonstrating a decrease in cell viability to 58.2±8.5% and curcumin an IC50 of 33±0.18μM. Furthermore confocal microscopy demonstrated nanoparticles of <500nm were able to accumulate within cells with FITC-loaded MSNP showing membrane localised and cytoplasmic accumulation following a 2h incubation. MSNP are useful carriers for poorly soluble phytochemicals and provide a novel vehicle to target and deliver drugs into the CNS and bypass the BBB through olfactory drug delivery
Physio-Pharmacological aspects of Guda Basti
Amongst all the Shodhan and Shaman treatments of Ayurveda; Basti is one which can be used in all types of diseases and conditions. With proper combination of different drug it acts as anabolic as well as catabolic therapeutics, that’s why it is called as half treatment. Basti is a medication given through rectum which is a part of G.I Tract hence enema is equated with Basti. When administration volume of enema exceeds 80-100ml as in Basti there is a possibility of migration of drugs in to the ascending colon and even up to the ileum. Hence pervading of Basti solution is most important consideration. G.I. tract from cecum to rectum is very much differing in anatomical as well as physiological point of view. So plant origin drugs given by this route shows different Physio-Pharmacological properties. Basti doesn’t act by virtue of its drug mixture alone, beside drug mixture; left lateral position of patient, time of Basti administration related with food, mixture of Madhu and Saindhav play an important role. The pervading attributes for Niruha and Anuvasan are different. On the basis of this attributes Physio-Pharmacological aspects can be classified under Absorption and Excretion. The main aim of this article is to collect all references of Basti/Rectal root of drug administration from modern as well as Ayurvedic point of view and to explore the exact mechanism of action. In future Niruha Basti may be used as beneficial method for removal of the body waste products and Anuvasan Basti more nearer regarding the new nutritional root
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Overcoming Parkinson’s disease: direct nose-to-brain delivery of amantadine
Treatments for Parkinson’s disease face significant challenges in delivery to the due to the presence of blood brain barrier (BBB). The aim of this study was to develop and characterise an in-situ thermoresponsive polymer based gel system for possible exploitation of the intranasal olfactory route where the BBB is absent. Reaching the olfactory region in the nasal cavity and mucociliary clearance are the main hurdles from formulation point of view. Thermo-sensitive and pH sensitive polymers seem to be the answer to these problems. Formulation made with these polymers along with mucoadhesives will be liquid at room temperature and will turn into a viscous gel when comes in contact with the body either due to change in pH or temperature. We have successfully prepared the gels using cold method using amantadine, with Pluronic F127 as a thermoreversible polymer and carboxymethylcelluose (CMS) as a mucoadhesive polymer. Rheological characterisation confirmed the sol-to-gel phenomenon at desired temperatures. CMC increased the gelation temperature with increase in concentration, whereas amantadine lowered the gelation temperature with increase in concentration. After preliminary studies, 16 % of Pluronic F127 found to be a suitable concentration for the formulation to change from sol-to-gel at ambient nasal temperatures. Molecular, gene expression and drug transport studies using the nasal epithelial cell lines RPMI 2650 are also under investigation along with the development of an in-vitro olfactory-epithelial cell culture model to study axonal transport. Unlike the animal studies this model will be faster to use, efficient and also better representation of human olfactory system
Phytochemical-loaded mesoporous silica nanoparticles for nose-to-brain olfactory drug delivery
Central nervous system (CNS) drug delivery is often hampered due to the insidious nature of the blood-brain barrier (BBB). Nose-to-brain delivery via olfactory pathways have become a target of attention for drug delivery due to bypassing of the BBB. The antioxidant properties of phytochemicals make them promising as CNS active agents but possess poor water solubility and limited BBB penetration. The primary aim of this study was the development of mesoporous silica nanoparticles (MSNs) loaded with the poorly water-soluble phytochemicals curcumin and chrysin which could be utilised for nose-to-brain delivery. We formulated spherical MSNP using a templating approach resulting in ∼220nm particles with a high surface porosity. Curcumin and chrysin were successfully loaded into MSNP and confirmed through Fourier transformation infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and HPLC approaches with a loading of 11-14% for curcumin and chrysin. Release was pH dependant with curcumin demonstrating increased chemical stability at a lower pH (5.5) with a release of 53.2%±2.2% over 24h and 9.4±0.6% for chrysin. MSNP were demonstrated to be non-toxic to olfactory neuroblastoma cells OBGF400, with chrysin (100μM) demonstrating a decrease in cell viability to 58.2±8.5% and curcumin an IC50 of 33±0.18μM. Furthermore confocal microscopy demonstrated nanoparticles of <500nm were able to accumulate within cells with FITC-loaded MSNP showing membrane localised and cytoplasmic accumulation following a 2h incubation. MSNP are useful carriers for poorly soluble phytochemicals and provide a novel vehicle to target and deliver drugs into the CNS and bypass the BBB through olfactory drug delivery