166 research outputs found
Thermodynamics of Surfactants, Block Copolymers and Their Mixtures in Water: The Role of the Isothermal Calorimetry
The thermodynamics of conventional surfactants, block copolymers and their mixtures in water was described to the light of the enthalpy function. The two methodologies, i.e. the vanât Hoff approach and the isothermal calorimetry, used to determine the enthalpy of micellization of pure surfactants and block copolymers were described. The vanât Hoff method was critically discussed. The aqueous copolymer+surfactant mixtures were analyzed by means of the isothermal titration calorimetry and the enthalpy of transfer of the copolymer from the water to the aqueous surfactant solutions. Thermodynamic models were presented to show the procedure to extract straightforward molecular insights from the bulk properties
A See-Saw model for fermion masses and mixings
We present a supersymmetric see-saw model giving rise to the most
general neutrino mass matrix compatible with Tri-Bimaximal mixing. We adopt the
flavour symmetry, broken by suitable vacuum expectation values
of a small number of flavon fields. We show that the vacuum alignment is a
natural solution of the most general superpotential allowed by the flavour
symmetry, without introducing any soft breaking terms. In the charged lepton
sector, mass hierarchies are controlled by the spontaneous breaking of the
flavour symmetry caused by the vevs of one doublet and one triplet flavon
fields instead of using the Froggatt-Nielsen U(1) mechanism. The next to
leading order corrections to both charged lepton mass matrix and flavon vevs
generate corrections to the mixing angles as large as .
Applied to the quark sector, the symmetry group can give a
leading order proportional to the identity as well as a matrix with
coefficients in the Cabibbo submatrix. Higher order
corrections produce non vanishing entries in the other entries which
are generically of .Comment: 30 pages, 3 figures, minor changes to match the published versio
A Simplest A4 Model for Tri-Bimaximal Neutrino Mixing
We present a see-saw model for Tri-Bimaximal mixing which is based on a
very economical flavour symmetry and field content and still possesses all the
good features of models. In particular the charged lepton mass
hierarchies are determined by the flavour symmetry itself
without invoking a Froggatt-Nielsen U(1) symmetry. Tri-Bimaximal mixing is
exact in leading order while all the mixing angles receive corrections of the
same order in next-to-the-leading approximation. As a consequence the predicted
value of is within the sensitivity of the experiments which will
take data in the near future. The light neutrino spectrum, typical of
see-saw models, with its phenomenological implications, also including
leptoproduction, is studied in detail.Comment: 20 pages, 2 figure
Self-assembled magnetic theranostic nanoparticles for highly sensitive MRI of minicircle DNA delivery
National Basic Research Program of China (973 Program) [2010CB732600, 2010CB732604]; National Natural Science Foundation of China [51203177]; Chinese Academy of Sciences; Guangdong Innovation Research Team Fund for Low-Cost Healthcare TechnologiesAs a versatile gene vector, minicircle DNA (mcDNA) has a great potential for gene therapy. However, some serious challenges remain, such as to effectively deliver mcDNA into targeted cells/tissues and to non-invasively monitor the delivery of the mcDNA. Superparamagnetic iron oxide (SPIO) nanoparticles have been extensively used for both drug/gene delivery and diagnosis. In this study, an MRI visible gene delivery system was developed with a core of SPIO nanocrystals and a shell of biodegradable stearic acid-modified low molecular weight polyethyleneimine (Stearic-LWPEI) via self-assembly. The Stearic-LWPEI-SPIO nanoparticles possess a controlled clustering structure, narrow size distribution and ultrasensitive imaging capacity. Furthermore, the nanoparticle can effectively bind with mcDNA and protect it from enzymatic degradation. In conclusion, the nanoparticle shows synergistic advantages in the effective transfection of mcDNA and non-invasive MRI of gene delivery
Long Non-Coding RNA XLOC_006753 Promotes the Development of Multidrug Resistance in Gastric Cancer Cells Through the PI3K/AKT/mTOR Signaling Pathway
Background/Aims: The development of multidrug resistance (MDR), which results in disease recurrence and metastasis, is a crucial obstacle to successful chemotherapy for patients with gastric cancer (GC). Long non-coding RNAs (lncRNAs) have been found to play various roles in cancer. This study aimed to investigate the effect of XLOC_006753 on the development of MDR in GC cells. Methods: The expression levels of XLOC_006753 in GC patients and MDR GC cell lines (SGC-7901/5-FU and SGC-7901/DDP cell line) were assessed by qRT-PCR. Statistical analyses were conducted to determine the relationship between XLOC_006753 expression and clinical features and to assess the prognostic value of XLOC_006753 for overall survival and progression-free survival. Then, a CCK-8 assay was used to detect cell proliferation ability and chemosensitivity. Flow cytometry was used to detect cell cycle and cell apoptosis. A wound-healing assay and transwell assay were used to detect cell migration. The expression of markers for MDR, G1/S transition, epithelialâmesenchymal transition (EMT) and PI3K/ AKT/mTOR signaling pathway were examined by western blot. Results: XLOC_006753 was highly expressed in GC patients and MDR GC cell lines (SGC-7901/5-FU and SGC-7901/DDP cell lines), and its high expression was positively associated with metastasis, TNM stage, tumor size, and poor survival in GC patients. Moreover, XLOC_006753 was an independent prognostic biomarker of overall survival and progression-free survival for gastric cancer patients. Knocking down XLOC_006753 in the two MDR GC cell lines significantly inhibited cell proliferation, cell viability, cell cycle G1/S transition, and migration. XLOC_006753 knockdown also promoted apoptosis. Furthermore, western blots showed that XLOC_006753 knockdown decreased some markers of MDR, G1/S transition, and EMT expression, while increasing caspase9 expression and inhibiting the PI3K/AKT/mTOR signaling pathway in SGC-7901/5-FU and SGC-7901/DDP cells. Conclusion: High expression of XLOC_006753 promoted the development of MDR, which was activated by the PI3K/AKT/mTOR pathway in GC cells
Neutrino physics from cosmological observations
We review the current status of neutrino cosmology, focusing mainly on the
question of the absolute values of neutrino masses and the possibility of a
cosmological neutrino lepton asymmetry.Comment: 7 pages, 3 figures, Invited talk at the XXth Internat. Conf. on
Neutrino Physics and Astrophysics (Neutrino 2002), Munich, Germany, May
25-30, 200
Theta_13: phenomenology, present status and prospect
The leptonic mixing angle theta_13 is currently a high-priority topic in the
field of neutrino physics, with five experiments under way, searching for
neutrino oscillations induced by this angle. We review the phenomenology of
theta_13 and discuss the information from present global oscillation data. A
description of the upcoming reactor and accelerator experiments searching for a
non-zero value of theta_13 is given, and we evaluate the sensitivity reach
within the next few years.Comment: Topical review, 55 pages, 23 figures, v2: various minor improvements,
references added, new section 6, matches version to appear in J. Phys.
Global patient outcomes after elective surgery: prospective cohort study in 27 low-, middle- and high-income countries.
BACKGROUND: As global initiatives increase patient access to surgical treatments, there remains a need to understand the adverse effects of surgery and define appropriate levels of perioperative care. METHODS: We designed a prospective international 7-day cohort study of outcomes following elective adult inpatient surgery in 27 countries. The primary outcome was in-hospital complications. Secondary outcomes were death following a complication (failure to rescue) and death in hospital. Process measures were admission to critical care immediately after surgery or to treat a complication and duration of hospital stay. A single definition of critical care was used for all countries. RESULTS: A total of 474 hospitals in 19 high-, 7 middle- and 1 low-income country were included in the primary analysis. Data included 44Â 814 patients with a median hospital stay of 4 (range 2-7) days. A total of 7508 patients (16.8%) developed one or more postoperative complication and 207 died (0.5%). The overall mortality among patients who developed complications was 2.8%. Mortality following complications ranged from 2.4% for pulmonary embolism to 43.9% for cardiac arrest. A total of 4360 (9.7%) patients were admitted to a critical care unit as routine immediately after surgery, of whom 2198 (50.4%) developed a complication, with 105 (2.4%) deaths. A total of 1233 patients (16.4%) were admitted to a critical care unit to treat complications, with 119 (9.7%) deaths. Despite lower baseline risk, outcomes were similar in low- and middle-income compared with high-income countries. CONCLUSIONS: Poor patient outcomes are common after inpatient surgery. Global initiatives to increase access to surgical treatments should also address the need for safe perioperative care. STUDY REGISTRATION: ISRCTN5181700
Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018.
Over the past decade, the Nomenclature Committee on Cell Death (NCCD) has formulated guidelines for the definition and interpretation of cell death from morphological, biochemical, and functional perspectives. Since the field continues to expand and novel mechanisms that orchestrate multiple cell death pathways are unveiled, we propose an updated classification of cell death subroutines focusing on mechanistic and essential (as opposed to correlative and dispensable) aspects of the process. As we provide molecularly oriented definitions of terms including intrinsic apoptosis, extrinsic apoptosis, mitochondrial permeability transition (MPT)-driven necrosis, necroptosis, ferroptosis, pyroptosis, parthanatos, entotic cell death, NETotic cell death, lysosome-dependent cell death, autophagy-dependent cell death, immunogenic cell death, cellular senescence, and mitotic catastrophe, we discuss the utility of neologisms that refer to highly specialized instances of these processes. The mission of the NCCD is to provide a widely accepted nomenclature on cell death in support of the continued development of the field
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