249 research outputs found
Purification and characterisation of the yeast plasma membrane ATP binding cassette transporter Pdr11p.
The ATP binding cassette (ABC) transporters Pdr11p and its paralog Aus1p are expressed under anaerobic growth conditions at the plasma membrane of the yeast Saccharomyces cerevisiae and are required for sterol uptake. However, the precise mechanism by which these ABC transporters facilitate sterol movement is unknown. In this study, an overexpression and purification procedure was developed with the aim to characterise the Pdr11p transporter. Engineering of Pdr11p variants fused at the C terminus with green fluorescent protein (Pdr11p-GFP) and containing a FLAG tag at the N terminus facilitated expression analysis and one-step purification, respectively. The detergent-solubilised and purified protein displayed a stable ATPase activity with a broad pH optimum near 7.4. Mutagenesis of the conserved lysine to methionine (K788M) in the Walker A motif abolished ATP hydrolysis. Remarkably, and in contrast to Aus1p, ATPase activity of Pdr11p was insensitive to orthovanadate and not specifically stimulated by phosphatidylserine upon reconstitution into liposomes. Our results highlight distinct differences between Pdr11p and Aus1p and create an experimental basis for further biochemical studies of both ABC transporters to elucidate their function
Einstein energy associated with the Friedmann -Robertson -Walker metric
Following Einstein's definition of Lagrangian density and gravitational field
energy density (Einstein, A., Ann. Phys. Lpz., 49, 806 (1916); Einstein, A.,
Phys. Z., 19, 115 (1918); Pauli, W., {\it Theory of Relativity}, B.I.
Publications, Mumbai, 1963, Trans. by G. Field), Tolman derived a general
formula for the total matter plus gravitational field energy () of an
arbitrary system (Tolman, R.C., Phys. Rev., 35(8), 875 (1930); Tolman, R.C.,
{\it Relativity, Thermodynamics & Cosmology}, Clarendon Press, Oxford, 1962));
Xulu, S.S., arXiv:hep-th/0308070 (2003)). For a static isolated system, in
quasi-Cartesian coordinates, this formula leads to the well known result , where is the
determinant of the metric tensor and is the energy momentum tensor of
the {\em matter}. Though in the literature, this is known as "Tolman Mass", it
must be realized that this is essentially "Einstein Mass" because the
underlying pseudo-tensor here is due to Einstein. In fact, Landau -Lifshitz
obtained the same expression for the "inertial mass" of a static isolated
system without using any pseudo-tensor at all and which points to physical
significance and correctness of Einstein Mass (Landau, L.D., and Lifshitz,
E.M., {\it The Classical Theory of Fields}, Pergamon Press, Oxford, 2th ed.,
1962)! For the first time we apply this general formula to find an expression
for for the Friedmann- Robertson -Walker (FRW) metric by using the same
quasi-Cartesian basis. As we analyze this new result, physically, a spatially
flat model having no cosmological constant is suggested. Eventually, it is seen
that conservation of is honoured only in the a static limit.Comment: By mistake a marginally different earlier version was loaded, now the
journal version is uploade
Energetics of the Einstein-Rosen spacetime
A study covering some aspects of the Einstein--Rosen metric is presented. The
electric and magnetic parts of the Weyl tensor are calculated. It is shown that
there are no purely magnetic E--R spacetimes, and also that a purely electric
E--R spacetime is necessarily static. The geodesics equations are found and
circular ones are analyzed in detail. The super--Poynting and the
``Lagrangian'' Poynting vectors are calculated and their expressions are found
for two specific examples. It is shown that for a pulse--type solution, both
expressions describe an inward radially directed flow of energy, far behind the
wave front. The physical significance of such an effect is discussed.Comment: 19 pages Latex.References added and updated.To appear in
Int.J.Theor.Phy
MADERA: A standardized Pan-Amazonian dataset for tropical timber species
We compiled and presented a dataset for all timber species reported in the Amazon region from all nine South American Amazonian countries. This was based on official information from every country, as well as from two substantial scientific references. We verified the standard taxonomic names from each individual source, using the Taxonomic Name Resolution Service (TNRS) and considered all Amazonian tree species with diameter at breast height (DBH) ≥10 cm. We also obtained estimates of the current population size for most species from a published approach based on data from 1900 tree inventory plots (1-ha each) distributed across the Amazon region and part from the Amazon Tree Diversity Network (ATDN). We then identified the hyperdominant timber species. In addition, we overlapped our timber species list with data for species that are used for commercial purposes, according to the International Tropical Timber Organization (ITTO), the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) and the International Union for Conservation of Nature (IUCN) taxa assessment and Red List categories. Finally, we also included IUCN Red List categories based on combined deforestation, and climate change scenarios for these species. Our final Amazonian timber species dataset contains 1112 unique species records, which belong to 337 genera and 72 families from the lowland Amazonian rainforest, with associated information related to population, conservation, and trade status of each species. The authors of this research expect that the information provided will be useful to strengthen the public forestry policies of the Amazon countries, inform ecological studies, as well for forest management purposes. The data are released under the Creative Commons Attribution 4.0 International license
Minimum mass-radius ratio for charged gravitational objects
We rigorously prove that for compact charged general relativistic objects
there is a lower bound for the mass-radius ratio. This result follows from the
same Buchdahl type inequality for charged objects, which has been extensively
used for the proof of the existence of an upper bound for the mass-radius
ratio. The effect of the vacuum energy (a cosmological constant) on the minimum
mass is also taken into account. Several bounds on the total charge, mass and
the vacuum energy for compact charged objects are obtained from the study of
the Ricci scalar invariants. The total energy (including the gravitational one)
and the stability of the objects with minimum mass-radius ratio is also
considered, leading to a representation of the mass and radius of the charged
objects with minimum mass-radius ratio in terms of the charge and vacuum energy
only.Comment: 19 pages, accepted by GRG, references corrected and adde
Stability and collapse of localized solutions of the controlled three-dimensional Gross-Pitaevskii equation
On the basis of recent investigations, a newly developed analytical procedure
is used for constructing a wide class of localized solutions of the controlled
three-dimensional (3D) Gross-Pitaevskii equation (GPE) that governs the
dynamics of Bose-Einstein condensates (BECs). The controlled 3D GPE is
decomposed into a two-dimensional (2D) linear Schr\"{o}dinger equation and a
one-dimensional (1D) nonlinear Schr\"{o}dinger equation, constrained by a
variational condition for the controlling potential. Then, the above class of
localized solutions are constructed as the product of the solutions of the
transverse and longitudinal equations. On the basis of these exact 3D
analytical solutions, a stability analysis is carried out, focusing our
attention on the physical conditions for having collapsing or non-collapsing
solutions.Comment: 21 pages, 14 figure
Quantitative importance of staminodes for female reproductive success in Parnassia palustris under contrasting environmental conditions.
The five sterile stamens, or staminodes, in Parnassia palustris act both as false and as true nectaries. They attract pollinators with their conspicuous, but non-rewarding tips, and also produce nectar at the base. We removed staminodes experimentally and compared pollinator visitation rate and duration and seed set in flowers with and without staminodes in two different populations. We also examined the relative importance of the staminode size to other plant traits. Finally, we bagged, emasculated, and supplementary cross-pollinated flowers to determine the pollination strategy and whether reproduction was limited by pollen availability. Flowers in both populations were highly dependent on pollinator visitation for maximum seed set. In one population pollinators primarily cross-pollinated flowers, whereas in the other the pollinators facilitated self-pollination. The staminodes caused increased pollinator visitation rate and duration to flowers in both populations. The staminodes increased female reproductive success, but only when pollen availability constrained female reproduction. Simple linear regression indicated a strong selection on staminode size, multiple regression suggested that selection on staminode size was mainly caused by correlation with other traits that affected female fitness. [ABSTRACT FROM AUTHOR
Anisotropic flow of charged hadrons, pions and (anti-)protons measured at high transverse momentum in Pb-Pb collisions at TeV
The elliptic, , triangular, , and quadrangular, , azimuthal
anisotropic flow coefficients are measured for unidentified charged particles,
pions and (anti-)protons in Pb-Pb collisions at TeV
with the ALICE detector at the Large Hadron Collider. Results obtained with the
event plane and four-particle cumulant methods are reported for the
pseudo-rapidity range at different collision centralities and as a
function of transverse momentum, , out to GeV/.
The observed non-zero elliptic and triangular flow depends only weakly on
transverse momentum for GeV/. The small dependence
of the difference between elliptic flow results obtained from the event plane
and four-particle cumulant methods suggests a common origin of flow
fluctuations up to GeV/. The magnitude of the (anti-)proton
elliptic and triangular flow is larger than that of pions out to at least
GeV/ indicating that the particle type dependence persists out
to high .Comment: 16 pages, 5 captioned figures, authors from page 11, published
version, figures at http://aliceinfo.cern.ch/ArtSubmission/node/186
Centrality dependence of charged particle production at large transverse momentum in Pb-Pb collisions at TeV
The inclusive transverse momentum () distributions of primary
charged particles are measured in the pseudo-rapidity range as a
function of event centrality in Pb-Pb collisions at
TeV with ALICE at the LHC. The data are presented in the range
GeV/ for nine centrality intervals from 70-80% to 0-5%.
The Pb-Pb spectra are presented in terms of the nuclear modification factor
using a pp reference spectrum measured at the same collision
energy. We observe that the suppression of high- particles strongly
depends on event centrality. In central collisions (0-5%) the yield is most
suppressed with at -7 GeV/. Above
GeV/, there is a significant rise in the nuclear modification
factor, which reaches for GeV/. In
peripheral collisions (70-80%), the suppression is weaker with almost independently of . The measured nuclear
modification factors are compared to other measurements and model calculations.Comment: 17 pages, 4 captioned figures, 2 tables, authors from page 12,
published version, figures at
http://aliceinfo.cern.ch/ArtSubmission/node/284
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