ESTRUTURA DA VEGETAÇÃO CONDICIONADA POR FATORES TOPOGRÁFICOS EM FLORESTA DE ENCOSTA NO PARNA – TIJUCA, RJ

O presente trabalho objetivou comparar a estrutura da vegetação entre diferentes posições na encosta e situações de declividades. O divisor apresentou vegetação menos robusta, com maior densidade de indivíduos, e com largura e altura consideravelmente mais baixas em relação às outras posições na encosta. A imposição de declividade parece influenciar o crescimento e forma das árvores, que tendem a ser mais baixas e com troncos mais largos, além de menor densidade, do que nas áreas planas

Meningite Crônica, Hidrocefalia E Paraplegia Medular Em Histoplasmose Não-sistêmica

74651451

Meningite crônica, hidrocefalia e paraplegia medular em histoplasmose não-sistêmica

[No abstract available]sem informação746514515sem informaçãosem informaçã

Proteínas do citoesqueleto na parede folicular de ovários normais e císticos de porcas matrizes

The expression of cytoskeletal proteins was evaluated immunohistochemically in 36 normal ovaries sampled from 18 sows and 44 cystic ovaries sampled from of 22 sows, was evaluated. All sows had history of reproductive problems, such as infertility or subfertility. The immunohistochemically stained area (IHCSA) was quantified through image analysis to evaluate the expression of these proteins in the follicular wall of secondary, tertiary, and cystic follicles. Cytokeratins (CK) immunoreactivity was strong in the granulosa cell layer (GC) and mild in the theca interna (TI) and externa (TE) of the normal follicles. There was severe reduction of the reaction to CK in the GC in the cystic follicles, mainly in the luteinized cysts. The immunoreactivity for vimentin was higher in the GC from normal and cystic follicles in contrast with the other follicular structures. In the luteinized cysts, the IHCSA for vimentin was significantly higher in TI and in both observed cysts, the labeling was more accentuated in TE. Immunohistochemical detection of desmin and α-SMA was restricted to the TE, without differences between the normal and cystic follicles. The results of the current study show that the development of ovarian cysts in sows is associated to changes in the expression of the cytoskeletal proteins CK and vimentin.A expressão de proteínas do citoesqueleto foi avaliada por imuno-histoquímica em ovários normais e císticos de porcas matrizes. Amostras de 36 ovários normais (18 porcas) e de 44 císticos (22 porcas) foram avaliadas. Todas as matrizes apresentaram histórico de problemas reprodutivos, como infertilidade ou subfertilidade. As áreas coradas por imuno-histoquímica (IHCSA) foram quantificadas por avaliação de imagens avaliando a expressão dessas proteínas na parede folicular de folículos secundários, terciários e císticos. A imuno-reatividade para citoqueratina (CK) foi forte na camada de células da granulosa (GC) e discreta nas tecas interna (TI) e externa (TE) dos folículos normais. Houve redução acentuada da reação de CK na CG dos folículos císticos, principalmente nos cistos luteinizados. A reação para vimentina foi mais intensa na CG dos folículos normais e císticos em comparação com outras estruturas foliculares. Nos cistos luteinizados, a IHCSA para vimentina foi significativamente maior na TI e, em ambos os cistos observados, a marcação foi mais acentuada na TE. A marcação de desmina e actina alfa de músculo liso foi restrita a TE, sem diferenças entre os folículos normais e císticos. Os resultados do presente estudo mostram que o desenvolvimento de cistos ovarianos em porcas matrizes está associado a alterações na expressão das proteínas do citoesqueleto CK e vimentina.Facultad de Ciencias Veterinaria

Safflower Oil: An Integrated Assessment Of Phytochemistry, Antiulcerogenic Activity, And Rodent And Environmental Toxicity

Gastric ulcers are a significant medical problem and the development of complications lead to significant mortality rates worldwide. In Brazil, Carthamus tinctorius L., Asteraceae, seeds essential oil, the safflower oil, is currently used as a thermogenic compound and as treatment for problems related to the cardiovascular system. In this study, by Raman spectroscopy, it was shown that oleic and linoleic acids are the compounds present in higher concentrations in the safflower oil. We demonstrated that safflower oil (750 mg/kg, p.o.) decrease the ulcerogenic lesions in mice after the administration of hydrochloric acid-ethanol. The gastric ulcers induced by non-steroidal anti-inflammatory drug (NSAID) in mice treated with cholinomimetics were treated with four different doses of safflower oil, of which, the dose of 187.5 mg/kg (p.o.) showed significant antiulcerogenic properties (p < 0.01). Moreover, the safflower oil at doses of 187.5 mg/kg (i.d.) increased the pH levels, gastric volume (p < 0.01) and gastric mucus production (p < 0.001), and decreased the total gastric acid secretion (p < 0.001). The acute toxicity tests showed that safflower oil (5.000 mg/kg, p.o.) had no effect on mortality or any other physiological parameter. Ecotoxicological tests performed using Daphnia similis showed an EC50 at 223.17 mg/l, and therefore safflower oil can be considered “non-toxic” based on the directive 93/67/EEC on risk assessment for new notified substances by European legislation. These results indicate that the antiulcer activity of Safflower oil may be due to cytoprotective effects, which serve as support for new scientific studies related to this pathology.245538544ABNT, Ecotoxicologia aquatica: Toxicidade aguda-metodo de ensaio com (2004) Daphnia, 21p. , spp (Cladocera, Crustacea). NBR 12713, Rio de JaneiroAmandeep, K., Robin, S., Ramica, S., Sunil, K., Peptic ulcer: A review of etiology and pathogenesis (2012) Int. J. Clin. Pharm, 3, pp. 34-38Arakawa, T., Watanabe, T., Tanigawa, T., Tominaga, K., Fujiwara, Y., Morimoto, K., Quality of ulcer healing in gastrointestinal tract: Its pathophysiology and clinical relevance (2012) World J. Gastroenterol, 18, pp. 4811-4822Bae, C.S., Park, C.H., Cho, H.J., Han, H.J., Kang, S.S., Choi, S.H., Uhm, C.S., Therapeutic effects of safflower (Carthamus tinctorius L.) seed powder on osteoporosis (2002) Korean J. Electron. Microscopy, 32, pp. 285-290Baeten, V., Dardenne, P., Aparicio, R., Interpretation of Fourier transform Raman spectra of the unsaponifiable matter in a selection of edible oils (2001) J. Agric. Food Chem, 49, pp. 5098-5107Brito, A.R.M.S., (1994) Manual de ensaios toxicologicos in vivo, 116p. , 1a ed., Campinas, Editora UnicampCEC, (1996) Technical guidance document in support of commission directive 93/67/EEC on risk assessment for new notified substances, , Part II, environmental risk assessment. Commission of the European Communities, Luxembourg: Office for official publication of the European CommunitiesCorley, D.A., Kubo, A., Zhao, W., Quesenberry, C., Proton pump inhibitors and histamine-2 receptor antagonists are associated with hip fractures among at-risk patients (2010) Gastroenterol, 139, pp. 93-101Deplege, M., Pharmaceuticals: Reduce drug waste in the environment (2011) Nature, 36, p. 478Fent, K., Weston, A.A., Caminada, D., Ecotoxicology of human pharmaceuticals (2006) Aquat. Toxicol, 76, pp. 122-159George, P., Concerns regarding the safety and toxicity of medicinal plants-An overview (2011) J. Appl. Pharm. Sci, 1, pp. 40-44Gilbert, N., Drug-pollution law all washed up (2012) Nature, 491, pp. 503-504Isidori, M., Parrella, A., Pistillo, P., Temussi, F., Effects of ranitidine and its photoderivatives in the aquatic environment (2009) Environ. Intern, 35, pp. 821-825Jain, K.S., Shah, A.K., Bariwal, J., Shelke, S.M., Kale, A.P., Jagtap, J.R., Bhosale, A.V., Recent advances in proton pump inhibitors and management of acid-peptic disorders (2007) Bioorg. Med. Chem, 15, pp. 1181-1205Kim, S.K., Cha, J.Y., Jeong, S.J., Chung, C.H., Choi, Y.R., Cho, Y.S., Properties of the chemical composition of safflower (Carthamus tinctorius L.) sprout (2000) Korean J. Life Sci, 10, pp. 68-73Madanick, R.D., Proton pump inhibitor side effects and drug interactions: Much ado about nothing? Cleve (2011) Clin. J. Med, 78, pp. 39-49Malfertheiner, P., Chan, F.K.L., McColl, K.E.L., Peptic ulcer disease (2009) Lancet, 374, pp. 1449-1461Mizui, T., Doteuchi, M., Effect of polyamines on acidified ethanol-induced gastric lesion in rats (1983) Jpn. J. Pharmacol, 33, pp. 939-945Olfert, D., Cross, B.M., McWilliam, A.A., (1993) Guide to the Care and Use of Experimental Animal, 213p. , Canadian Council on Animal Care, OntarioPintao, A.M., Da Silva, I.F., A verdade sobre o acafrao (2008) Workshop Plantas Medicinais e Fitoterapeuticas nos Tropicos. IICT/CCCM, 29, pp. 30, 31. , de Outubro, 1-19Rainsford, K.D., The effects of aspirin and other non-steroid anti-inflammatory/analgesic drugs on gastro-intestinal mucus glycoprotein biosynthesis in vivo: Relationship to ulcerogenic actions (1978) Biochem. Pharmacol, 27, pp. 877-885Rudolphi, S., Becker, H.C., Schierholt, A., Witzke-Ehbrecht, S.V., Improved estimation of oil, linoleic and oleic acid and seed hull fractions in safflower by NIRS.J (2012) Am. Oil Chem. Soc, 89, pp. 363-369Santos, L.H.L.M., Araujo, A.N., Fachinia, A., Pena, A., Delerue-Matos, C., Montenegro, M.C.B.S.M., Ecotoxicological aspects related to the presence of pharmaceuticals in the aquatic environment (2010) J. Hazard Mater, 175, pp. 45-95Saraf, A., Saraf, S., Legal regulations of complementary and alternative medicines in different countries (2012) Pharmacogn. Rev, 6, pp. 154-160Shay, H., Komarov, S.A., Fels, S.S., Meranze, D., Gruenstein, M., Siplet, H., A simple method for the uniform production of gastric ulceration in the rat (1945) Gastroenterol, 5, pp. 43-61Silveira, F.L., Silveira, L., Jr., Villaverde, A.B., Pacheco, M.T.T., Pasqualucci, C.A., Use of dispersive Raman Spectroscopy in the determination of unsaturated fat in commercial edible oil-and fat-containing industrialized foods (2010) Instrum. Sci. Technol, 38, pp. 107-123Silveira, L., Silveira, F.L., Bodanese, B., Zangaro, R.A., Pacheco, M.T.T., Discriminating model for diagnosis of basal cell carcinoma and melanoma in vitro based on the Raman spectra of selected biochemicals (2012) J. Biomed. Opt, 17Stachowska, E., Dolegowska, B., Dziedziejk, V., Rybicka, M., Kaczmarczyh, M., Bober, J., Rac, M., Chlubek, D., Prostaglandin E2 (PGE2) and thromboxane A2 (TXA2) synthesis is regulated by conjugate linoleic acids (CLA) in human macrophages (2009) J. Physiol. Pharmacol, 60, pp. 77-85Sun, X.B., Matsumoto, T., Yamada, H., Effects of a polysaccharide fraction from the root of Bupleurum falcatum L on experimental gastric ulcer models in rats and mice (1991) J. Pharm. Pharmacol, 43, pp. 669-704Szelenyi, I., Thiemer, K., Distention ulcer as a model for testing of drugs for ulcerogenic side effects (1978) Arch. Toxicol, 41, pp. 99-105Takeuchi, H., Matsuo, T., Tokuyama, K., Shimomura, Y., Suzuki, M., Diet-induced thermogenesis is lower in rats fed a lard diet than in those fed a high oleic acid safflower oil diet, a safflower oil diet or a linseed oil diet (1995) J. Nutr, 125, pp. 920-925US EPA, (2002) Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms, 266p. , 5th ed. United States Environmental Protection Agency, EPA-821-R-02-012Webb, S., A data-based perspective on the environmental risk assessment of human pharmaceuticals I. collation of available ecotoxicity data and II. aquatic risk characterisation (2001) Pharmaceuticals in the environment sources, fate, effects and risks, pp. 175-230. , Kummerer K editor. 1st ed. Heidelberg, Germany: Springer-Verlag pWHO, (1997) Adverse Drug Reaction Database, , World Health Organization, Uppsala Sweden. 36. Institute of Safe Medication Practices Medication Safety Alert. June 4, V 01. 2 No. llWHO, (2002) Monographs on the Evaluation of Carcinogenic Risks to Humans, , (Some Traditional Herbal Medicines), World Health Organization Vol. 82, IARC Press Lyon, FranceZapata-Colindres, J.C., Zepeda-Gomez, S., Montano-Loza, A., Jesus-Villalobos, J.E., Valdovinos-Andraca, F., The association of Helicobacter pylori infection and nonsteroidal anti-inflammatory drugs in peptic ulcer disease (2006) Can. J. Gastroenterol, 20, pp. 277-28

Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water

The transport of live fish is a routine practice in aquaculture and constitutes a considerable source of stress to the animals. The addition of anesthetic to the water used for fish transport can prevent or mitigate the deleterious effects of transport stress. This study investigated the effects of the addition of eugenol (EUG) (1.5 or 3.0 mu L L-1) and essential oil of Lippia alba (EOL) (10 or 20 mu L L-1) on metabolic parameters (glycogen, lactate and total protein levels) in liver and muscle, acetylcholinesterase activity (AChE) in muscle and brain, and the levels of protein carbonyl (PC), thiobarbituric acid reactive substances (TBARS) and nonprotein thiol groups (NPSH) and activity of glutathione-S-transferase in the liver of silver catfish (Rhamdia quelen; Quoy and Gaimard, 1824) transported for four hours in plastic bags (loading density of 169.2 g L-1). The addition of various concentrations of EUG (1.5 or 3.0 mu L L-1) and EOL (10 or 20 mu L L-1) to the transport water is advisable for the transportation of silver catfish, since both concentrations of these substances increased the levels of NPSH antioxidant and decreased the TBARS levels in the liver. In addition, the lower liver levels of glycogen and lactate in these groups and lower AChE activity in the brain (EOL 10 or 20 mu L L-1) compared to the control group indicate that the energetic metabolism and neurotransmission were lower after administration of anesthetics, contributing to the maintenance of homeostasis and sedation status.Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS/PRONEX) [10/0016-8]; Conselho Nacional de Pesquisa e Desenvolvimento Cientifico (CNPq) [470964/2009-0]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES); CNPqinfo:eu-repo/semantics/publishedVersio

Search for a W' boson decaying to a bottom quark and a top quark in pp collisions at sqrt(s) = 7 TeV

Results are presented from a search for a W' boson using a dataset corresponding to 5.0 inverse femtobarns of integrated luminosity collected during 2011 by the CMS experiment at the LHC in pp collisions at sqrt(s)=7 TeV. The W' boson is modeled as a heavy W boson, but different scenarios for the couplings to fermions are considered, involving both left-handed and right-handed chiral projections of the fermions, as well as an arbitrary mixture of the two. The search is performed in the decay channel W' to t b, leading to a final state signature with a single lepton (e, mu), missing transverse energy, and jets, at least one of which is tagged as a b-jet. A W' boson that couples to fermions with the same coupling constant as the W, but to the right-handed rather than left-handed chiral projections, is excluded for masses below 1.85 TeV at the 95% confidence level. For the first time using LHC data, constraints on the W' gauge coupling for a set of left- and right-handed coupling combinations have been placed. These results represent a significant improvement over previously published limits.Comment: Submitted to Physics Letters B. Replaced with version publishe