60 research outputs found
The Agrobacterium VirE3 effector protein: a potential plant transcriptional activator
During the infection of plants, Agrobacterium tumefaciens introduces several Virulence proteins including VirE2, VirF, VirD5 and VirE3 into plant cells in addition to the T-DNA. Here, we report that double mutation of virF and virE3 leads to strongly diminished tumor formation on tobacco, tomato and sunflower. The VirE3 protein is translated from a polycistronic mRNA containing the virE1, virE2 and virE3 genes, in Agrobacterium. The VirE3 protein has nuclear localization sequences, which suggests that it is transported into the plant cell nucleus upon translocation. Indeed we show here that VirE3 interacts in vitro with importin-α and that a VirE3âGFP fusion protein is localized in the nucleus. VirE3 also interacts with two other proteins, viz. pCsn5, a component of the COP9 signalosome and pBrp, a plant specific general transcription factor belonging to the TFIIB family. We found that VirE3 is able to induce transcription in yeast when bound to DNA through the GAL4-BD. Our data indicate that the translocated effector protein VirE3 is transported into the nucleus and there it may interact with the transcription factor pBrp to induce the expression of genes needed for tumor development
Social media in democratic transitions and consolidations: what can we learn from the case of Tunisia?
© 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group. The aim of this paper is to analyse the use of social media in the stages of uprising, democratic transition and democratic consolidation using the case study of Tunisia. While the impact of social media in uprisings has been widely documented in past research about the MENA region, Tunisia provides new evidence to the use of Internet in the processes of democratisation. Consequently, this research focuses in detail on the benefits but also the pitfalls of social media in transitions and consolidations. Data collection was based on interviews with Tunisian social media activists. The analysis is valuable to social media practitioners and researchers alike
Recommended from our members
The global history of the Balfour Declaration: declared nation By Maryanne A. Rhett. Abingdon: Routledge, 2016. Pp. xiv + 168. Hardback ÂŁ110.00, ISBN 978-1-138-11941-3.
- âŠ