Quantification of aluminum-induced changes in wheat root architecture by X-ray microcomputed tomography

Root architectural traits are of fundamental importance for plant performance, especially under unfavorable soil conditions. This study examined the effect of aluminum (Al) toxicity in different growing media (nutrient solutions and soil) on root architecture of two wheat (Triticum aestivum L.) cultivars with different Al tolerances. Seedlings were grown in acidic and limed soil and in two contrasting nutrient solutions. Root systems of soil-grown plants were scanned using x-ray microcomputed tomography (µCT) while that of nutrient solution–grown plants were assesses using WinRhizo, 3 and 5 days after planting (DAP), respectively. Aluminum caused significant reduction of all examined root traits (number of seminal roots, root length, length of the longest seminal root, root surface area, and root volume). Growth in acidic soil caused significant reduction in root length, length of the longest seminal root, and root surface area at 5 DAP. Soil-grown plants produced a larger root system compared to plants grown in nutrient solutions. Aluminum toxicity–induced differences of root traits were also found between different nutrient solutions. Beside the well-known reduction of root length, Al toxicity had a profound effect on other root architectural traits. X-ray µCT has revealed root architectural changes under specific conditions of acidic, Al-toxic soil. Differences obtained in Al-induced effects on root architecture between different nutrient solutions as well as between different growing systems emphasize the need for further study of root architecture, especially under specific conditions of Al toxicity in acidic soils

Exploring AGN Activity over Cosmic Time with the SKA

In this Chapter we present the motivation for undertaking both a wide and deep survey with the SKA in the context of studying AGN activity across cosmic time. With an rms down to 1 $\mu$Jy/beam at 1 GHz over 1,000 - 5,000 deg$^2$ in 1 year (wide tier band 1/2) and an rms down to 200 nJy/beam over 10 - 30 deg$^2$ in 2000 hours (deep tier band 1/2), these surveys will directly detect faint radio-loud and radio-quiet AGN (down to a 1 GHz radio luminosity of about $2\times10^{23}$ W/Hz at $z=6$). For the first time, this will enable us to conduct detailed studies of the cosmic evolution of radio AGN activity to the cosmic dawn ($z\gtrsim6$), covering all environmental densities.Comment: 17 pages, 7 figures, to appear as part of 'Continuum Science' in Proceedings 'Advancing Astrophysics with the SKA (AASKA14)

Star-forming galaxies versus low- and high-excitation radio AGN in the VLA-COSMOS 3GHz Large Project

We study the composition of the faint radio population selected from the VLA-COSMOS 3GHz Large Project, a radio continuum survey performed at 10 cm wavelength. The survey covers the full 2 square degree COSMOS field with mean $rms\sim2.3$ $\mu$Jy/beam, cataloging 10,899 source components above $5\times rms$. By combining these radio data with UltraVISTA, optical, near-infrared, and Spitzer/IRAC mid-infrared data, as well as X-ray data from the Chandra Legacy, and Chandra COSMOS surveys, we gain insight into the emission mechanisms within our radio sources out to redshifts of $z\sim5$. From these emission characteristics we classify our souces as star forming galaxies or AGN. Using their multi-wavelength properties we further separate the AGN into sub-samples dominated by radiatively efficient and inefficient AGN, often referred to as high- and low-excitation emission line AGN. We compare our method with other results based on fitting of the sources' spectral energy distributions using both galaxy and AGN spectral models, and those based on the infrared-radio correlation. We study the fractional contributions of these sub-populations down to radio flux levels of $\sim$10 $\mu$Jy. We find that at 3 GHz flux densities above $\sim$400 $\mu$Jy quiescent, red galaxies, consistent with the low-excitation radio AGN class constitute the dominant fraction. Below densities of $\sim$200 $\mu$Jy star-forming galaxies begin to constitute the largest fraction, followed by the low-excitation, and X-ray- and IR-identified high-excitation radio AGN.Comment: 7 pages, 3 figures, The many facets of extragalactic radio surveys: towards new scientific challenges, Bologna 20-23 October 201

MicroRNA399 is a long-distance signal for the regulation of plant phosphate homeostasis

The presence of microRNA species in plant phloem sap suggests potential signaling roles by long-distance regulation of gene expression. Proof for such a role for a phloem-mobile microRNA is lacking. Here we show that phosphate (Pi) starvation-induced microRNA399 (miR399) is present in the phloem sap of two diverse plant species, rapeseed and pumpkin, and levels are strongly and specifically increased in phloem sap during Pi deprivation. By performing micro-grafting experiments using Arabidopsis, we further show that chimeric plants constitutively over-expressing miR399 in the shoot accumulate mature miR399 species to very high levels in their wild-type roots, while corresponding primary transcripts are virtually absent in roots, demonstrating shoot-to-root transport. The chimeric plants exhibit (i) down-regulation of the miR399 target transcript (PHO2), which encodes a critical component for maintenance of Pi homeostasis, in the wild-type root, and (ii) Pi accumulation in the shoot, which is the phenotype of pho2 mutants, miR399 over-expressers or chimeric plants with a genetic knock-out of PHO2 in the root. Hence the transported miR399 molecules retain biological activity. This is a demonstration of systemic control of a biological process, i.e. maintenance of plant Pi homeostasis, by a phloem-mobile microRNA